Animal Sciences Commons^™

A Non-Invasive Assay For Monitoring Stress Responses: A Comparison Between Wild And Captive-Reared Rainbowfish (Melanoteania Duboulayi), Amina Zuberi, Sinan Ali, Culum Brown

Aquaculture Collection

The stress response of wild and captive reared rainbowfish (Melanoteania duboulayi) following chasing by a simulated predator was examined. Cortisol release rate was monitored using a flow through system by measuring water borne hormone levels. Tests using known cortisol concentrations revealed that the technique yielded 95% of the cortisol present in the water. Cortisol release rates increased several fold in both populations after being chased but peaked at different time periods. Wild fish showed a typical stress response with release rate rising to (2.29±0.22 ng g⁻¹ h⁻¹) 2 h after exposure followed by rapid recovery. The captive-reared …

Detection Of Two Pathogens Of High Importance To The National Poultry Improvement Plan: Salmonella Spp. And Mycoplasma Spp., Robin Levi Jarquin

Graduate Theses and Dissertations

In 1935, the National Poultry Improvement Plan (NPIP) was created to control Salmonella gallinarum and Salmonella pullorum . These two pathogens were devastating economically for poultry producers. Through cooperative efforts using vaccination and strict biosecurity, these two pathogens were eradicated from the United States. Currently, the NPIP program is targeting two other poultry pathogens, Salmonella enterica serovar Enteritidis and Mycoplasma . In the broiler industry it targets 2 specific Mycoplasma species (synoviae, gallisepticum). Vaccinations for these bacteria are available, but are not fully effective at controlling all strains and serovars. Thus, constant monitoring systems and strict biosecurity measures are necessary …

Protein Utilization And Requirements In Broiler Breeders, Ricardo David Ekmay

Graduate Theses and Dissertations

Advances in the genetics of poultry have created unintended consequences to the broiler breeder industry. Excessive weight has been shown to have many negative effects, including double hierarchies, multiple ovulations, and fatty liver. In an effort to curb excessive body weight gain, both the protein requirements and feeding strategies of broiler breeders are being revisited. A series of studies were conducted to determine the protein and amino acid requirements of broiler breeders during the production phase and to determine the interrelationship between protein turnover and egg formation. In the first trial, a 2x3x3 factorial study was conducted to determine the …

African Elephants Change Gaits When Walking Downhill, Robert H.I. Dale, Robert Warren, Brock Ward, Eric Noble

Scholarship and Professional Work - LAS

Abstract from the International Elephant and Rhino Conservation and Research Symposium, Rotterdam, NL, October 10-14, 2011.

Heifer Estrous Cycle Stage Impacts Luteinizing And Growth Hormone Response To Kisspeptin, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Barbara P. Steele, James L. Sartin

Animal Science Publications and Other Works

The reproductive neuropeptide, kisspeptin (KP), may have a role in regulation of growth hormone (GH) secretion. Expression of KP and its receptor (Kiss1r) are developmentally and hormonally regulated. This study was conducted to determine the effects of KP on plasma concentrations of luteinizing hormone (LH) and GH in pubertal heifers during different functional reproductive stages. Heifers received a single injection of KP (100 pmole / kg) or saline intravenously during proestrus, estrus, and diestrus, and serial blood samples were collected for 3 hours to determine the response of both LH and GH after treatment. Mean concentrations of LH were increased …

Growing South Dakota (Fall 2011), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

Page] 2 Adapting for the Future: South Dakota 4-H Efforts Move Forward
[Page] 5 Regional Transitions: Eight Extension Centers to Open in October
[Page] 6 College News [Page] 8 Making a Difference with Research: Ag Experiment Station Aids Industry & Economy
[Page] 10 Pig Potential: South Dakota's Swine Industry Offers Economic Opportunity [Page] 13 New Department Debuts: Natural Resource Disciplines Join Forces
[Page] 14 Autumn in the AIr: Grab Your Blue and Gold and Visit Campus
[Page] 16 iGrow Evolves: Usability And Content Are Being Enhanced
[Page] 18 Collection Effort: Despite Budget Cuts, Private Funds Allowed for Growth [Page] 21 …

Heifer Estrous Cycle Stage Impacts Luteinizing And Growth Hormone Response To Kisspeptin, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Barbara P. Steele, James L. Sartin

Brian K Whitlock, PhD, DVM, DACT

The reproductive neuropeptide, kisspeptin (KP), may have a role in regulation of growth hormone (GH) secretion. Expression of KP and its receptor (Kiss1r) are developmentally and hormonally regulated. This study was conducted to determine the effects of KP on plasma concentrations of luteinizing hormone (LH) and GH in pubertal heifers during different functional reproductive stages. Heifers received a single injection of KP (100 pmole / kg) or saline intravenously during proestrus, estrus, and diestrus, and serial blood samples were collected for 3 hours to determine the response of both LH and GH after treatment. Mean concentrations of LH were increased …

Surveillance Of Selected Diseases In Free-Ranging Elk (Cervus Elaphus Nelsoni) In Nebraska, 1995-2009, Michael A. Cover, Scott E. Hygnstrom, David W. Oates, Kit M. Hams, Kurt C. Vercauteren

Great Plains Research: A Journal of Natural and Social Sciences

Sera samples were collected from 21 free-ranging, captured female elk (Cervus elaphus nelsoni) in 1995- 96, and tissue and sera samples were collected from 415 hunter-harvested elk from 1995 to 2006 and tested for selected diseases. Titers for Anaplasma marginale were detected in 81 of 436 (19%) elk. Occurrence of antibodies to anaplasmosis increased from 4 to 40 elk from 2002 to 2006. Titers for bovine viral diarrhea virus (BVDV) were detected in 18 of 346 (5%) samples. Titers for Leptospira interrogans serovars were detected in 21 of 289 (7%) of samples from 1995 to 2004. Titers for …

Effect Of Kisspeptin On Regulation Of Growth Hormone, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Herris S. Maxwell, Barbara P. Steele, James L. Sartin

Animal Science Publications and Other Works

Kisspeptin ( KP ), a neuroendocrine regulator of reproduction, is hypothesized to be an integrator of metabolism and hormones critical to the regulation of reproduction. Lactation is associated with enhanced growth hormone (GH) responsiveness and reduced fertility. Our study was designed to determine the effects of lactation on KP-stimulated GH and luteinizing hormone (LH) secretion. Five non-lactating and five lactating dairy cows were used in the study. Experiments were conducted with lactating cows at weeks 1, 5 and 11 after parturition. The experimental treatments ( saline and KP [100 and 400 pmol / kg body weight]) were given intravenously and …

Effect Of Kisspeptin On Regulation Of Growth Hormone, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Herris S. Maxwell, Barbara P. Steele, James L. Sartin

Brian K Whitlock, PhD, DVM, DACT

Kisspeptin ( KP ), a neuroendocrine regulator of reproduction, is hypothesized to be an integrator of metabolism and hormones critical to the regulation of reproduction. Lactation is associated with enhanced growth hormone (GH) responsiveness and reduced fertility. Our study was designed to determine the effects of lactation on KP-stimulated GH and luteinizing hormone (LH) secretion. Five non-lactating and five lactating dairy cows were used in the study. Experiments were conducted with lactating cows at weeks 1, 5 and 11 after parturition. The experimental treatments ( saline and KP [100 and 400 pmol / kg body weight]) were given intravenously and …

Efecto Del Almacenamiento De Huevo Incubable Con La Cámara De Aire Hacia Abajo, Sobre La Incubabilidad, Según La Edad De Las Reproductoras, Para Tres Días De Rotación, Marco Guío, Jorge Briñez, Jorge Amado, Andrés Martínez

Revista Ciencia Animal

Actualmente, las líneas de conformación han reducido presión sobre la producción de huevos por parte de las abuelas y reproductoras pesadas, mientras se han concentrado en el rendimiento del pollo de engorde, evidenciándose mejores conversiones alimenticias en ambos sexos. Este contexto expone el hecho de que, en Colombia, las empresas más exitosas, que se encuentran entre el 5% y el 25% de productividad del país, con dificultad podrán superar los resultados históricos. Se hace necesario analizar todas las oportunidades de mejora en el manejo que contribuyan a maximizar los rendimientos zootécnicos de las aves, para obtener la mayor cantidad posible …

The Efficacy Of Three Types Of Analgesic Drugs In Reducing Pain In The Rainbow Trout, Oncorhynchus Mykiss, Jessica J. Mettam, Lois J. Oulton, Catherine R. Mccrohan, Lynne U. Sneddon

Veterinary Science and Medicine Collection

Recent research has shown the possibility of pain perception in fish; therefore, the use of analgesia or “painkillers” should be considered for invasive procedures. However, there is relatively little information on the effectiveness of analgesic drugs nor on the appropriate dose for fish. This study assessed the efficacy of three types of drug: an opioid, buprenorphine, a non-steroidal anti-inflammatory drug (NSAID), carprofen and a local anaesthetic, lidocaine. Each drug was tested at three doses on rainbow trout that were noxiously stimulated and the most effective dose was also given to fish experiencing no pain to investigate side-effects. Ventilation rate and …

Spiking Patterns And Their Functional Implications In The Antennal Lobe Of The Tobacco Hornworm Manduca Sexta, Hong Lei, Carolina E. Reisenman, Caroline H. Wilson, Prasad Gabbur, John G. Hildebrand

Health Sciences and Kinesiology Faculty Articles

Bursting as well as tonic firing patterns have been described in various sensory systems. In the olfactory system, spontaneous bursts have been observed in neurons distributed across several synaptic levels, from the periphery, to the olfactory bulb (OB) and to the olfactory cortex. Several in vitro studies indicate that spontaneous firing patterns may be viewed as “fingerprints” of different types of neurons that exhibit distinct functions in the OB. It is still not known, however, if and how neuronal burstiness is correlated with the coding of natural olfactory stimuli. We thus conducted an in vivo study to probe this question …

The Use Of Chloramines To Eradicate Quagga Mussel Larvae, Trea Lacroix, Kumud Acharya

Undergraduate Research Opportunities Program (UROP)

Quagga Mussels, Dressenia bugensis, are a growing problem in the western United States, particularly in their ability to infest underwater infrastructures and clog water intake pipes and screens of power and treatment plants. Chlorine has been found to be the most effective chemical to get rid of veligers (planktonic larval form of quagga mussels) in the pipes. However, chlorine leaves a residue called trihalomethane, which is a carcinogen at higher concentrations. The purpose of this project is to test the effectiveness of an alternate chemical, chloramines (chlorine and ammonia), which leaves behind little to no residual trihalomethane. Upon experimentation with …

The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, Brian K. Whitlock, Joseph A. Daniel, Maria Hes, Barbara P. Steele, James L. Sartin, S. Oishi, N. Fujii

Animal Science Publications and Other Works

Kisspeptin receptor (Kiss1r) agonists with increased half-life and similar efficacy to kisspeptin (KP) in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test the effect of a Kiss1r agonist (FTM080) on luteinizing hormone (LH) in vivo. Sheep were treated with FTM080 (500 pmol/kg BW) or sterile water in a 2-ml bolus via the jugular cannula. Serial blood samples were collected every 15-min before (1 hr) and after (1 hr) treatment. Intravenous (IV) injection of FTM080 increased (P < 0.05) LH plasma concentrations through the 45-min sample following treatment. Moreover, the area under the curve of LH in the period from 0 to 60-min following FTM080 treatment was also increased (P < 0.05). These data provide evidence to suggest that FTM080 stimulates the gonadotropic axis of ruminants in vivo.

The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, Brian K. Whitlock, Joseph A. Daniel, Maria Hes, Barbara P. Steele, James L. Sartin, S. Oishi, N. Fujii

Brian K Whitlock, PhD, DVM, DACT

Kisspeptin receptor (Kiss1r) agonists with increased half-life and similar efficacy to kisspeptin (KP) in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test the effect of a Kiss1r agonist (FTM080) on luteinizing hormone (LH) in vivo. Sheep were treated with FTM080 (500 pmol/kg BW) or sterile water in a 2-ml bolus via the jugular cannula. Serial blood samples were collected every 15-min before (1 hr) and after (1 hr) treatment. Intravenous (IV) injection of FTM080 increased (P < 0.05) LH plasma concentrations through the 45-min sample following treatment. Moreover, the area under the curve of LH in the period from 0 to 60-min following FTM080 treatment was also increased (P < 0.05). These data provide evidence to suggest that FTM080 stimulates the gonadotropic axis of ruminants in vivo.

Variation In Parenting Style In A Captive Group Of Western Lowlands Gorillas, Ana E. Fernandez

Theses and Dissertations - UTB/UTPA

Parenting styles were examined in a group of western lowland gorillas to determine the relationship between parenting style and multiple births with a single individual. Another interest in this study was on varying ways that motherhood can be approached, with partial attention to the relationship between parenting style and social rank. Observational data was collected by means of focal animal sampling and used to analyze contact behaviors in mother-infant relationship. Results indicated that parenting style stayed consistent within the same mother, but different between mothers.

Phenotypic Divergence Despite High Levels Of Gene Flow In Galapagos Lava Lizards (Microlophus Albemarlensis), Mark Jordan, H. Snell, H. Snell, W. Jordan

Mark A. Jordan

The extent of evolutionary divergence of phenotypes between habitats is predominantly the result of the balance of differential natural selection and gene flow. Lava lizards (Microlophus albemarlensis) on the small island of Plaza Sur in the Galápagos archipelago inhabit contrasting habitats: dense vegetation on the western end of the island thins rapidly in a transitional area, before becoming absent on the eastern half. Associated with these habitats are phenotypic differences in traits linked to predator avoidance (increased wariness, sprint speed, and endurance in lizards from the sparsely vegetated habitat). This population provides an opportunity to test the hypothesis that reduced …

Historical Fragmentation And Genetic Drift In Populations Of Galápagos Lava Lizards (Microlophus Albemarlensis Complex), Mark Jordan, H. Snell

Mark A. Jordan

No abstract provided.

Volume 8, Number 1 (Spring/Summer 2011), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Planting a Garden of Hope for Cancer Patients
• In Smith County, a Community Reinvents Itself
• Advancing Tennessee Through Strategic Extension

Multistate Mark-Recapture Analysis Reveals No Effect Of Blood Sampling On Survival And Recapture Of Eastern Kingbirds (Tyrannus Tyrannus), Lucas J. Redmond, Michael T. Murphy

Biology Faculty Publications and Presentations

The experimentally supported and prevailing opinion is that blood sampling has few to no long-term effects on survival of birds when conducted properly, and blood sampling has become a vital addition to the toolbox of many ornithologists. However, many of the studies that concluded that blood sampling had negligible effects on birds used approaches that did not account for temporary emigration and probability of capture. To date, the only study to have done so found that blood sampling had a strong negative effect on survival. We conducted a mark–recapture analysis of 8 years of banding and bleeding data on Eastern …

Results Of A Preliminary Shoreline Shellfish Sanitary Survey Near Banjul, Gambia Conducted On 18 June 2011, Kanyi Babanding, Michael A. Rice

Michael A Rice

A preliminary shoreline survey was conducted along the Bund Road (frequently referred to as Bond Road) levee and the Banjul-Serrekunda Highway in the northern Tanbi Wetlands oyster harvesting area in the metropolitan Banjul area. In part this was designed as a training exercise for Gambian officials will be assuming responsibility for future shoreline surveys in the Gambia. Key identified areas of concern include the Banjul fish landing area on Bund Road utilized by a number of artisanal fishers; an over-water toilet facility for fishers near the fish landing site; evidence of illegal waste and rubbish dumping along Bund Road; an …

Mitigation Of Bovine Respiratory Disease Complex: Interactions Of Physiology, Immunology, Nutrition And Management, John Thomas Richeson

Graduate Theses and Dissertations

A series of experiments were conducted to determine the effects of on-arrival vs. delayed respiratory vaccination and exposure to persistently infected bovine viral diarrhea virus challenge on health, gain performance, and physiological and immunological measurements in newly received stocker cattle. Two experiments evaluated timing (d 0 vs. 14) of respiratory, clostridial, or both vaccinations in newly received stocker calves during stress-induced immunosuppression. In Exp. 1, calves receiving 14-d delayed vaccination of a pentavalent modified-live respiratory virus (MLV) vaccine had greater ADG during the 42-d receiving period and antibody titers against infectious bovine rhinotracheitis on d 42; however, morbidity rate did …

A Mark–Recapture Technique For Monitoring Feral Swine Populations, Matthew M. Reidy, Tyler A. Campbell, David G. Hewitt

USDA Wildlife Services: Staff Publications

Techniques to monitor populations of feral swine (Sus scrofa) relative to damage control activities are needed on rangelands. Our objectives were to describe and assess a mark–recapture technique using tetracycline hydrochloride (TH) for monitoring feral swine populations. We established bait stations at study sites in southern and central Texas. During 1 d, we replaced normal soured corn bait with bait containing TH and counted the number of feral swine that consumed bait with observers. We conducted feral swine removal using box-style traps and helicopters, at which time we collected teeth for TH analysis. In southern Texas, we estimated …

Regulation Of Immediate Early And Epithelial-Mesenchymal Transition Genes Expression By Adipocytokines In The Female Reproductive Tract, Zhufeng Yang

Department of Animal Science: Dissertations, Theses, and Student Research

Individuals who are overweight or obese are at increased risk for cancer. However, the mechanistic link between obesity and cancer is poorly defined. Adipose tissue produces hormones and pro-inflammatory cytokines with mitogenic properties. Many of these hormones and cytokines are altered in obese individuals and may lead to disruption of the normal balance between cell proliferation, differentiation, and apoptosis. Thus, the objective of this study was to determine how adipocyte-derived factors regulate the expression of genes that contribute to cell proliferation and migration. To compare immediate early gene (cell proliferation) and epithelial-mesenchymal transition (cell migration) gene expression between untreated and …

A Prevalence Study Of Southeast Origin Sale Barn Beef Cattle, Comingled In Warren County, Kentucky, Persistently Infected With Bovine Viral Diarrhea Virus, Including The Effects Of Season And Body Weight, Sarah Elizabeth Thomas

Masters Theses & Specialist Projects

Bovine viral diarrhea (BVD) is an economically important disease of cattle. Calves persistently infected (PI) with the bovine viral diarrhea virus (BVDV) are a powerful agent for spread of the virus. A total of 24,423 southeast origin beef cattle comingled at three Warren County, Kentucky locations were tested from November 2007 to June 2010 for PI BVDV. A total of 97 head tested positive for PI BVDV, giving an average overall prevalence of 0.397%.
Calves tested were subdivided into categories for additional calculations of dependence. A total of 8,910 were categorized by weight range upon testing (300-399 lbs, 400-499 lbs, …

Estimation Of The Number Of Days Required To Determine Usual Antioxidant Intakes And Assessment Of The Prevalence Of Nutrient Inadequacy Among College Students, Catherine G. Davis

Master's Theses

High intake of antioxidant rich foods has been shown to decrease risk factors of chronic disease. Young adulthood may be crucial in establishing healthy lifestyles including adequate nutrient consumption.

The present study was designed 1) to estimate usual nutrient intakes, 2) to calculate the number of days required to estimate usual antioxidant intake, and 3) to assess intake adequacy from diet and diet + supplement sources by using the Estimated Average Requirement (EAR). The USDA Flavonoid and Proanthocyanidin databases, food consumption data, and dietary supplement use data from 60 students aged 18-25 years at the University of Connecticut were utilized. …

Growing South Dakota (Spring 2011), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Classroom to Careers: An Optimistic Outlook Awaits Ag & Biological Sciences Graduates
[Page] 5 New Opportunities: Intro Ag Courses To Be Offered in Rapid City
[Page] 6 Budget Reality: Funding Cuts Prompt Structure and Staff Changes
[Page] 7 Gearing Up for DakotaFest: Replicated SDSU Campus, Educational Forums and Fundraising Auction Planned
[Page] 8 Continuing a Legacy: &1 Million Livestock Judging Endowment Being Established
[Page] 10 4-H Fun: Unique 4-H Activities Spark Student Interest in Science
[Page] 12 Innovative Outreach: BBQ Boot Camp Connect Consumers to Meat Industry
[Page] 13 College News
[Page] 14 Relevant Research: New, More Efficient …

Evolution Of A Major Drug Metabolizing Enzyme Defect In The Domestic Cat And Other Felidae: Phylogenetic Timing And The Role Of Hypercarnivory, Binu Shrestha, J. Michael Reed, Philip T. Starks, Gretchen E. Kaufman, Jared V. Goldstone, Melody E. Roelke, Stephen J. O'Brien, Klaus-Peter Koepfli, Laurence Frank, Michael H. Court

Biology Faculty Articles

The domestic cat (Felis catus) shows remarkable sensitivity to the adverse effects of phenolic drugs, including acetaminophen and aspirin, as well as structurally-related toxicants found in the diet and environment. This idiosyncrasy results from pseudogenization of the gene encoding UDP-glucuronosyltransferase (UGT) 1A6, the major species-conserved phenol detoxification enzyme. Here, we established the phylogenetic timing of disruptive UGT1A6 mutations and explored the hypothesis that gene inactivation in cats was enabled by minimal exposure to plant-derived toxicants. Fixation of the UGT1A6 pseudogene was estimated to have occurred between 35 and 11 million years ago with all extant Felidae having dysfunctional …

Assessment Of Pathways For The Introduction And Spread Of Mycobacterium Bovis In The United States, Katie Portacci, Jason Lombard, Lauren Abrahamsen, Eric Bush, Charles Fossler, Robert Harris, Kamina Johnson, Ryan S. Miller, Dianna Mitchell, Randy Pritchard, Steven Sweeney, Todd Weaver

Other Publications in Zoonotics and Wildlife Disease

Bovine tuberculosis (TB) was responsible for more losses among U.S. farm animals in the early 20th century than all other infectious diseases combined. The Cooperative State-Federal Tuberculosis Eradication Program (established in 1917 and administered by APHIS, State animal health agencies, and U.S. livestock producers) has nearly eradicated bovine TB from the nation’s livestock population. However, despite the many accomplishments of the program, bovine TB remains a serious and costly disease of livestock in the United States. In 1992, VS conducted an assessment to identify pathways for the introduction and spread of bovine TB, in order to develop the most effective …