Animal Sciences Commons^™

Breed Differences In Placental Development During Late Gestation Between Chinese Meishan And White Crossbred Gilts In Response To Intrauterine Crowding, Jeremy R. Miles, Jeffrey L. Vallet

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The objective of this study was to evaluate placental development during late gestation (day 100) between Chinese Meishan (CM; n = 7) and White crossbred (WC; n = 5) gilts following intrauterine crowding induced by unilaterally hysterectomy-ovariectomy. Gross placental morphology and areolae density as well as histological morphology (i.e., folded bilayer and placental stroma) were analyzed using computer-assisted morphometry for placentas of the smallest and largest fetuses within each litter. There was a breed by fetal size interaction (P < 0.01) for areolae density in which placentas for large CM fetuses had greater areolae density compared to small CM fetuses, but the density of areolae was greater for CM fetuses compared to WC fetuses, irrespective of fetal size. The width of the folded bilayer was greater (P < 0.01) in placentas for WC gilts compared to CM gilts, irrespective of fetal size. Placentas for small fetuses had greater (P < 0.01) folded bilayer width compared to large fetuses, irrespective of breed. The placental stromal width was greater (P < 0.01) in placentas for large fetuses compared to small, irrespective of breed. The difference between stromal width in placentas between divergent-sized littermates, however, was greater (P = 0.05) in WC gilts compared to CM gilts, indicating there was a limited response to intrauterine crowding in CM gilts. These results indicate there is an altered placental development during late gestation in CM compared to WC gilts, thus, there are likely different mechanisms for responding to intrauterine crowding between breeds.

The Heritability Of Pampiniform Plexus Vessel Size And Varicocoele In Boars, Tasha R. Gruhot, Lea A. Rempel, Matthew L. Spangler, Stephen D. Kachman, Benny E. Mote

Department of Animal Science: Faculty Publications

Ultrasonography was used to capture a coronal–sagittal image of the veins of the pampiniform plexus (PP) and the testicular artery of 327 maternal‐line boars at approximately 6 months of age at the University of Nebraska—Lincoln. Varicocoele was diagnosed by two methods. Method 1 diagnosed varicocoele when the average vessel area on one side of the scrotum was 1.5 times larger than the average vessel area on the other side of the scrotum. Method 2 diagnosed varicocoele when the average vessel area on one side of the scrotum of a boar was 1.5 times larger than the average vessel on the …

The Impact Of Truncating Data On The Predictive Ability For Single-Step Genomic Best Linear Unbiased Prediction, Jeremy T. Howard, Thomas A. Rathje, Caitlyn E. Bruns, Danielle F. Wilson-Wells, Stephen D. Kachman, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Simulated and swine industry data sets were utilized to assess the impact of removing older data on the predictive ability of selection candidate estimated breeding values (EBV) when using single-step genomic best linear unbiased prediction (ssGBLUP). Simulated data included thirty replicates designed to mimic the structure of swine data sets. For the simulated data, varying amounts of data were truncated based on the number of ancestral generations back from the selection candidates. The swine data sets consisted of phenotypic and genotypic records for three traits across two breeds on animals born from 2003 to 2017. Phenotypes and genotypes were iteratively …

Fecal Microbiota Transplantation Is Associated With Reduced Morbidity And Mortality In Porcine Circovirus Associated Disease, Megan C. Niederwerder, Laura A. Constance, Raymond R. R. Rowland, Waseem Abbas, Samodha C. Fernando, Megan L. Potter, Maureen A. Sheahan, Thomas E. Burkey, Richard A. Hesse, Ada G. Cino-Ozuna

Department of Animal Science: Faculty Publications

Porcine circovirus associated disease (PCVAD) is a term used to describe the multifactorial disease syndromes caused by porcine circovirus type 2 (PCV-2), which can be reproduced in an experimental setting through the co-infection of pigs with PCV-2 and porcine reproductive and respiratory syndrome virus (PRRSV). The resulting PCVAD-affected pigs represent a subpopulation within the co-infected group. In co-infection studies, the presence of increased microbiome diversity is linked to a reduction in clinical signs. In this study, fecal microbiota transplantation (FMT) was investigated as a means to prevent PCVAD in pigs co-infected with PRRSV and PCV-2d. The sources of the FMT …

Genomics Symposium: Using Genomic Approaches To Uncover Sources Of Variation In Age At Puberty And Reproductive Longevity In Sows, Hiruni R. Wijesena, Clay A. Lents, Jean-Jack Riethoven, Melanie D. Trenhaile-Grannemann, Jennifer F. Thorson, Brittney N. Keel, Phillip S. Miller, Matt Spangler, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Genetic variants associated with traits such as age at puberty and litter size could provide insight into the underlying genetic sources of variation impacting sow reproductive longevity and productivity. Genomewide characterization and gene expression profiling were used using gilts from the University of Nebraska–Lincoln swine resource population (n = 1,644) to identify genetic variants associated with age at puberty and litter size traits. From all reproductive traits studied, the largest fraction of phenotypic variation explained by the Porcine SNP60 BeadArray was for age at puberty (27.3%). In an evaluation data set, the predictive ability of all SNP from highranked 1-Mb …

Development Of An Objective Feet And Leg Conformation Evaluation Method Using Digital Imagery In Swine, J. D. Stock, J. A. Calderon Diaz, C. E. Abell, T. J. Baas, M. F. Rothschild, B. E. Mote, K. J. Stalder

Department of Animal Science: Faculty Publications

Background:The objectives of this study were to create an objective measurement method of joint angles for knee, hock, front and rear pasterns and a rear stance position in swine using digital imaging technology and to assess the repeatability of the objective measurement process. Methods and Findings: Forty-five multiparous sows (average parity 6.7 ± 2.5; parity range 5 to 14) from two commercial farms (n=21 farm 1 and n=24 farm 2) were used. Sows were moved to a pen where digital images of the profile and rear stance were captured. On average, 5.2 (± 2.6) profile and 2.6 (± 1.0) …

Genomic Analysis Of Sow Reproductive Traits: Identification Of Selective Sweeps, Major Genes, And Genotype By Diet Interactions, Melanie D. Trenhaile

Department of Animal Science: Dissertations, Theses, and Student Research

Reproductive traits, such as litter size and reproductive longevity, are economically important. However, selection for these traits is difficult due to low heritability, polygenic nature, sex-limited expression, and expression late in life. Marker-assisted selection may provide an alternative to increase genetic progress.

Nebraska Index Line (NIL) has been selected for litter size related traits since 1981. It is one of the main contributing lines to the UNL reproductive longevity resource population (n > 1,500), which was genotyped for 60,000 SNPs, phenotyped for age at puberty (AP), lifetime number of parities (LTNP), litter size traits, and other reproductive traits, and fed either …

Evaluation Of Reduced Subsets Of Single Nucleotide Polymorphisms For The Prediction Of Age At Puberty In Sows, Katherine L. Lucot, Matthew L. Spangler, Melanie D. Trenhaile, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Genomic information could be used efficiently to improve traits that are expensive to measure, sex limited or expressed late in life. This study analyzed the phenotypic variation explained by major SNPs and windows for age at puberty in gilts, an indicator of reproductive longevity. A genome-wide association study using 56,424 SNPs explained 25.2% of the phenotypic variation in age at puberty in a training set (n = 820). All SNPs from the top 10% of 1-Mb windows explained 33.5% of the phenotypic variance compared to 47.1% explained by the most informative markers (n = 261). In an evaluation population, consisting …

Genome-Wide Analysis Of Tnf-Alpha Response In Pigs Challenged With Porcine Circovirus 2b, C. A. Kreikemeier, T. B., K. L. Lucot, Thomas E. Burkey, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with a role in activating adaptive immunity to viral infections. By inhibiting the capacity of plasmacytoid dendritic cells to produce interferon-α and TNF-α, porcine circovirus 2 (PCV₂) limits the maturation of myeloid dendritic cells and impairs their ability to recognize viral and bacterial antigens. Previously, we reported QTL for viremia and immune response in PCV₂- infected pigs. In this study, we analyzed phenotypic and genetic relationships between TNFα protein levels, a potential indicator of predisposition to PCV₂ co-infection, and PCV₂ susceptibility. Following experimental challenge with …

Effect Of Lysozyme Or Antibiotics On Faecal Zoonotic Pathogens In Nursery Pigs, J E. Wells, E. D. Berry, N Kalchayanand, L A. Rampel, M Kim, W. T. Oliver

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aims: The objective of this study was to determine the effect of lysozyme and antibiotics on zoonotic pathogen shedding in faeces from nursery pigs housed without and with an indirect disease challenge. Methods and Results: Two replicates of approximately 650 pigs each were weaned and randomly assigned to one of 24 pens in either a nursery room that had been fully disinfected or a nursery room left unclean. Pigs were randomly assigned to control diet (Control), control diet + antibiotics (Antibiotic; chlortetracycline and tiamulin), or control diet + lysozyme (Lysozyme; 100 mg kg ^-1 diet). Rectal swab samples were collected …

Effect Of Hydrogen Peroxide In The Scald Tank On The Microbial Count Of Pork Skin, A. S. De Mello Jr., R. O. Roca

Department of Animal Science: Faculty Publications

The objective of this study was to investigate the effect of hydrogen peroxide (H₂O₂) (50% v/v) on pork skin microbial populations. Forty-eight crossbred hogs were analyzed after dehairing and 30 during chilling. Three different concentrations of hydrogen peroxide were added to the scalding water (0.01, 0.05 and 0.1% of the total capacity of the scald tank). In treatment I no addition of H₂O₂ was applied while, in treatment II, H₂O₂ was added at 0 min and, in treatment III, it was added at 0, 30 and 60 min. Both treatments …

Ec75-219 Nebraska Swine Report, William Ahlschwede, T. E. Socha, Alfonso Torres-Medina, A. J. Lewis, P. J. Cunningham, Dwane R. Zimmerman, E. R. Peo Jr., Larry K. Mcmullen, Bobby D. Moser, D. L. Ferguson, Phillip H. Grabouski, Murray Danielson, Charles W. Francis, L. F. Elliott, J. A. Deshazer, Roger W. Mandigo, W. J. Goldner, R. D. Fritschen, Gary Zoubek

University of Nebraska-Lincoln Extension: Historical Materials

This 1975 Nebraska Swine Report was prepared by the staff in Animal Science and cooperating departments for use in the Extension and Teaching programs at the University of Nebraska-Lincoln. Authors from the following areas contributed to this publication: Swine Nutrition, swine diseases, pathology, economics, engineering, swine breeding, meats, agronomy, and diagnostic laboratory. It covers the following areas: breeding, disease control, feeding, nutrition, economics, housing and meats.