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Full-Text Articles in Animal Sciences
Applications Of Transposable Elements In Fish For Transgenesis And Functional Genomics, Perry B. Hackett, Karl J. Clark, Stephen C. Ekker, Jeffrey J. Essner
Applications Of Transposable Elements In Fish For Transgenesis And Functional Genomics, Perry B. Hackett, Karl J. Clark, Stephen C. Ekker, Jeffrey J. Essner
Jeffrey J. Essner
Transgenic fish were first made more than 30 years ago. Since then a variety of methods and const ructs have been tested for introducing genetic sequences into fish for scientitlc investigations as well as commercial purposes. Here we review transposable clements and t heir applications in fish. Transposons can be used to deliver genes to chromosomes to confer new traits or as insertional agents and traps to uncover the functions and expression patterns of natural genes in chromosomes. Two DNA transposons have been characterized for transposon-based gene transfer and insertional mutagenesis. The t1rst is d1e Sleeping Beauty transposon system that …
Predicting The Growth Of Salmonella Typhimurium On Beef By Using The Temperature Function Integration Technique, James S. Dickson, G. R. Siragusa, J. E. Wray Jr.
Predicting The Growth Of Salmonella Typhimurium On Beef By Using The Temperature Function Integration Technique, James S. Dickson, G. R. Siragusa, J. E. Wray Jr.
James S. Dickson
Lag and generation times for the growth of Salmonella typhimurium on sterile lean beef were modeled as functions of cooling time under various carcass-chilling scenarios. Gompertz growth models were fit to the log10 colony counts over time at each of six temperatures in the range of 15 to 40 degrees C. Lag and generation times were defined as the points at which the second and first derivatives, respectively, of each growth curve attained a maximum. Generation time and lag time parameters were modeled as functions of temperature by use of exponential-decay models. The models were applied to typical beef carcass-cooling …
Use Of A Bacteriocin Produced By Pediococcus Acidilactici To Inhibit Listeria Monocytogenes Associated With Fresh Meat, J. W. Nielsen, James S. Dickson, J. D. Crouse
Use Of A Bacteriocin Produced By Pediococcus Acidilactici To Inhibit Listeria Monocytogenes Associated With Fresh Meat, J. W. Nielsen, James S. Dickson, J. D. Crouse
James S. Dickson
A bacteriocin produced by Pediococcus acidilactici had an inhibitory and bactericidal effect on Listeria monocytogenes associated with fresh meat. MICs were significantly lower than minimum killing concentrations. When meat was inoculated with L. monocytogenes, the bacteriocin reduced the number of attached bacteria in 2 min by 0.5 to 2.2 log cycles depending upon bacteriocin concentration. Meat treated initially with the bacteriocin resulted in attachment of 1.0 to 2.5 log cycles fewer bacteria than that attained with the control. The bacteriocin, after 28 days of refrigerated storage on meat surfaces, was stable and exhibited an inhibitory effect on L. monocytogenes.
Cell Surface Charge Characteristics And Their Relationship To Bacterial Attachment To Meat Surfaces, James S. Dickson, M. Koohmaraie
Cell Surface Charge Characteristics And Their Relationship To Bacterial Attachment To Meat Surfaces, James S. Dickson, M. Koohmaraie
James S. Dickson
Cell surface charge and hydrophobicity of Bacillus subtilis, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Serratia marcescens, Staphylococcus aureus, and Staphylococcus epidermidis were determined by hydrocarbon adherence, hydrophobic interaction, and electrostatic interaction chromatography. Surface charge and hydrophobicity were compared with the initial attachment values and rates of attachment of the bacteria to meat surfaces. There was a linear correlation between the relative negative charge on the bacterial cell surface and initial attachment to lean beef muscle (r2 = 0.885) and fat tissue (r2 = 0.777). Hydrophobicity correlated well with attachment to fat tissue only. The relative hydrophobicity of each bacterium …