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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
- Discipline
- Keyword
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- Phaseolus vulgaris (4)
- Cattle (3)
- Drought stress (3)
- Biofloc technology production system (2)
- Canis lupus (2)
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- Channel catfish (2)
- Mycobacterium avium subsp. paratuberculosis (2)
- Wolf (2)
- 2-methylisoborneol and geosmin off-flavors (1)
- Andean (1)
- BLAST (1)
- Basic Local Alignment Search Tool; ELISA (1)
- Biofloc technology (1)
- Bos taurus (1)
- Bovine (1)
- Cattle depredations (1)
- Common bean (1)
- Cooking time (1)
- Cover crops (1)
- Crop production (1)
- Cytotoxic T cells (1)
- Dry beans (1)
- Encounter risk (1)
- End‐use quality (1)
- Enzyme-linked immunosorbent assay; GBS (1)
- Field phenotyping (1)
- Flow cytometry (1)
- GPS collars (1)
- Gene Pool (1)
- Gene pool (1)
Articles 1 - 25 of 25
Full-Text Articles in Agriculture
Management Controls The Net Greenhouse Gas Outcomes Of Growing Bioenergy Feedstocks On Marginally Productive Croplands, Virginia L. Jin, Marty Schmer, Catherine E. Stewart, Robert B. Mitchell, Candiss O. Williams, Brian J. Wienhold, Gary Varvel, Ronald F. Follett, John Kimble, Kenneth P. Vogel
Management Controls The Net Greenhouse Gas Outcomes Of Growing Bioenergy Feedstocks On Marginally Productive Croplands, Virginia L. Jin, Marty Schmer, Catherine E. Stewart, Robert B. Mitchell, Candiss O. Williams, Brian J. Wienhold, Gary Varvel, Ronald F. Follett, John Kimble, Kenneth P. Vogel
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Bio-based energy is key to developing a globally sustainable low-carbon economy. Lignocellulosic feedstock production on marginally productive croplands is expected to provide substantial climate mitigation benefits, but long-term field research comparing greenhouse gas (GHG) outcomes during the production of annual versus perennial crop-based feedstocks is lacking. Here, we show that long-term (16 years) switchgrass (Panicum virgatum L.) systems mitigate GHG emissions during the feedstock production phase compared to GHG-neutral continuous corn (Zea mays L.) under conservation management on marginally productive cropland. Increased soil organic carbon was the major GHG sink in all feedstock systems, but net agronomic GHG outcomes …
Comparison Of Unused Water And Year-Old Used Water For Production Of Channel Catfish In The Biofloc Technology System, Bartholomew W. Green, Kevin K. Schrader, Steven D. Rawles, Carl D. Webster, Matthew E. Mcentire
Comparison Of Unused Water And Year-Old Used Water For Production Of Channel Catfish In The Biofloc Technology System, Bartholomew W. Green, Kevin K. Schrader, Steven D. Rawles, Carl D. Webster, Matthew E. Mcentire
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Since excreted feed nitrogen is bio-transformed efficiently in a fully functional mixotrophic biofloc technology production system, re-using this biofloc water over multiple production cycles should be beneficial. The present study, conducted in an outdoor biofloc technology production system, evaluated impacts on fish production characteristics and mineral status, common microbial off-flavors, and water quality dynamics for channel catfish (Ictalurus punctatus) reared in one-year-old waters with low or high total suspended solids used previously for two consecutive catfish biofloc studies or in unused (new) water. Total suspended solids were maintained at 300 to 400 mg/L in the unused and low …
Emergence Of Common Lambsquarters (Chenopodium Album L.) Is Influenced By The Landscape Position In Which Seeds Developed, Sharon K. Papiernik, Frank Forcella, Gary B. Amundson
Emergence Of Common Lambsquarters (Chenopodium Album L.) Is Influenced By The Landscape Position In Which Seeds Developed, Sharon K. Papiernik, Frank Forcella, Gary B. Amundson
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
In a 2-yr field study, we evaluated the emergence and early growth of Chenopodium album L. (common lambsquarters) seedlings as affected by the landscape position in which the seeds (i) developed, (ii) overwintered, and (iii) were planted. Results indicated that a higher proportion of seeds originating from lower slope positions emerged compared with seeds originating from the backslope or upper slope. The timing of emergence was the same for all seed source locations. There was no influence of overwintering location on weed emergence. Regardless of the seed source, we observed faster emergence and growth of C. album planted in the …
Single And Multi-Trait Gwas Identify Genetic Factors Associated With Production Traits In Common Bean Under Abiotic Stress Environments, Atena Oladzad, Timothy G. Perch, Juan Carlos Rosas, Samira Mafi Moghaddam, James Beaver, Steve E. Beebe, Jimmy Burridge, Celestina Nhagupana Jochua, Magalhaes Amade Miguel, Phillip N. Miklas, Bodo Raatz, Jeffery W. White, Jonathan Lynch, Phillip E. Mcclean
Single And Multi-Trait Gwas Identify Genetic Factors Associated With Production Traits In Common Bean Under Abiotic Stress Environments, Atena Oladzad, Timothy G. Perch, Juan Carlos Rosas, Samira Mafi Moghaddam, James Beaver, Steve E. Beebe, Jimmy Burridge, Celestina Nhagupana Jochua, Magalhaes Amade Miguel, Phillip N. Miklas, Bodo Raatz, Jeffery W. White, Jonathan Lynch, Phillip E. Mcclean
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The genetic improvement of economically important production traits of dry bean (Phaseolus vulgaris L.), for geographic regions where production is threatened by drought and high temperature stress, is challenging because of the complex genetic nature of these traits. Large scale SNP data sets for the two major gene pools of bean, Andean and Middle American, were developed by mapping multiple pools of genotype-by-sequencing reads and identifying over 200k SNPs for each gene pool against the most recent assembly of the P. vulgaris genome sequence. Moderately sized Bean Abiotic Stress Evaluation (BASE) panels, consisting of genotypes appropriate for production in …
Sugarcane Straw Blanket Management Effects On Plant Growth, Development, And Yield In Southeastern Brazil, Izaias P. Lisboa, Maurício R. Cherubin, Renato P. De Lima, Maria R. Gmach, Brian J. Wienhold, Marty R. Schmer, Virginia L. Jin, Elizio F.F. Junior, Henrique P. Guerra, Carlos C. Cerri, Carlos E.P. Cerri
Sugarcane Straw Blanket Management Effects On Plant Growth, Development, And Yield In Southeastern Brazil, Izaias P. Lisboa, Maurício R. Cherubin, Renato P. De Lima, Maria R. Gmach, Brian J. Wienhold, Marty R. Schmer, Virginia L. Jin, Elizio F.F. Junior, Henrique P. Guerra, Carlos C. Cerri, Carlos E.P. Cerri
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
In Brazilian sugarcane (Saccharum spp.) production systems, the practice of moving harvesting residue from row to inter-row positions (i.e., raking) has increased in response to producer concerns over the potential negative effects of sugarcane straw on crop establishment and stalk yield. Despite increasing adoption among sugarcane farmers, the impacts of straw raking practices on plant growth and yield remain unclear. A 2-yr experiment that included both dry and wet seasons was conducted at two sites in southeastern Brazil to evaluate straw management strategy effects on plant tillering, phytomass accumulation, plant nutritional status, and stalk yield. The experiments were established at …
Development Of Single Nucleotide Polymorphism Markers For The Wheat Curl Mite Resistance Gene Cmc4, Jixin Zhao, Nader R. Abdelsalam, Luaay Khalaf, Wen-Po Chuang, Lanfei Zhao, C. Michael Smith, Brett Carver, Guihua Bai
Development Of Single Nucleotide Polymorphism Markers For The Wheat Curl Mite Resistance Gene Cmc4, Jixin Zhao, Nader R. Abdelsalam, Luaay Khalaf, Wen-Po Chuang, Lanfei Zhao, C. Michael Smith, Brett Carver, Guihua Bai
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Wheat curl mite (Aceria tosichella Keifer) is an important wheat (Triticum aestivum L. em. Thell.) pest in many wheat-growing regions worldwide. Mite feeding damage not only directly affects wheat yield, but A. tosichella also transmits Wheat streak mosaic virus (WSMV). Wheat resistance to A. tosichella, therefore, helps control WSMV. OK05312 (PI 670019) is an advanced breeding line released from Oklahoma that shows a high level of A. tosichella resistance. To map the gene(s) conditioning wheat resistance to A. tosichella in OK05312, a genetic linkage map was constructed using single nucleotide polymorphism (SNP) markers derived from genotyping-by-sequencing (GBS) and a population …
Next-Generation Sequencing Identifies Polyunsaturated Fatty Acid Responsive Genes In The Juvenile Rat Cerebellum, Aaron A. Mehus, Aaron M. Dickey, Timothy P. L. Smith, Kathleen M. Yeater, Matthew Picklo
Next-Generation Sequencing Identifies Polyunsaturated Fatty Acid Responsive Genes In The Juvenile Rat Cerebellum, Aaron A. Mehus, Aaron M. Dickey, Timothy P. L. Smith, Kathleen M. Yeater, Matthew Picklo
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Dietary n-3 polyunsaturated fatty acids (PUFA) influence postnatal brain growth and development. However, little data exist regarding the impacts of dietary n-3 PUFA in juvenile animals post weaning, which is a time of rapid growth. We tested the hypothesis that depleting dietary n-3 PUFA would result in modifications to the cerebellar transcriptome of juvenile rats. To test this hypothesis, three week old male rats (an age that roughly corresponds to an 11 month old child in brain development) were fed diets containing either soybean oil (SO) providing 1.1% energy from α-linolenic acid (ALA; 18:3n-3; ALA-sufficient) or corn oil (CO) providing …
Effects Of Dietary Protein Content On Hybrid Tilapia (Oreochromis Aureus × O. Niloticus) Performance, Common Microbial Off-Flavor Compounds, And Water Quality Dynamics In An Outdoor Biofloc Technology Production System, Bartholomew W. Green, Steven D. Rawles, Kevin K. Schrader, T. Gibson Gaylord, Matthew E. Mcentire
Effects Of Dietary Protein Content On Hybrid Tilapia (Oreochromis Aureus × O. Niloticus) Performance, Common Microbial Off-Flavor Compounds, And Water Quality Dynamics In An Outdoor Biofloc Technology Production System, Bartholomew W. Green, Steven D. Rawles, Kevin K. Schrader, T. Gibson Gaylord, Matthew E. Mcentire
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Given tilapia grown in the biofloc technology production system can consume the biofloc, it should be possible to optimize formulated diet protein content to account for nutrition derived from consuming biofloc. The present study, conducted in an outdoor biofloc technology production system, evaluated impacts on fish production indices, common microbial off-flavors, and water quality dynamics for hybrid tilapia (Oreochromis aureus×O. niloticus) fed diets formulated to contain 22.5%, 27.7%, and 32.3% digestible protein (DP) and 6% lipid. Fingerlings (32.2 ± 10.1 g/fish) were stocked in tanks (18.6m2; 16.6m3) in May 2016 at 25/m2 (29/m3) and grown for 5 months to market …
A Long‑Term Precision Agriculture System Sustains Grain Profitability, Matt Yost, Newell R. Kitchen, Kenneth A. Sudduth, R. E. Massey, E. J. Sadler, S. T. Drummond, M. R. Volkman
A Long‑Term Precision Agriculture System Sustains Grain Profitability, Matt Yost, Newell R. Kitchen, Kenneth A. Sudduth, R. E. Massey, E. J. Sadler, S. T. Drummond, M. R. Volkman
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
After two decades of availability of grain yield-mapping technology, long-term trends in field-scale profitability for precision agriculture (PA) systems and conservation practices can now be assessed. Field-scale profitability of a conventional or ‘business-as-usual’ system with an annual corn (Zea mays L.)-soybean (Glycine max [L.]) rotation and annual tillage was assessed for 11 years on a 36 ha field in central Missouri during 1993 to 2003. Following this, a ‘precision agriculture system’ (PAS) with conservation practices was implemented for the next 11 years to address production, profit and environmental concerns. The PAS was multifaceted and temporally dynamic. It included no-till, cover …
Unmanned Aerial System And Satellite-Based High Resolution Imagery For High-Throughput Phenotyping In Dry Bean, Sindhu Sankaran, Juan José Quirós, Phillip N. Miklas
Unmanned Aerial System And Satellite-Based High Resolution Imagery For High-Throughput Phenotyping In Dry Bean, Sindhu Sankaran, Juan José Quirós, Phillip N. Miklas
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Dry bean breeding programs are crucial to improve the productivity and resistance to biotic and abiotic stress. Phenotyping is a key process in breeding that refers to crop trait evaluation. In recent years, high-throughput plant phenotyping methods are being developed to increase the accuracy and efficiency for crop trait evaluations. In this study, aerial imagery at different resolutions were evaluated to phenotype crop performance and phenological traits using genotypes from two breeding panels, Durango Diversity Panel (DDP) and Andean Diversity Panel (ADP). The unmanned aerial system (UAS) based multispectral and thermal data were collected for two seasons at multiple time …
Seedling Root Architecture And Its Relationship With Seed Yield Across Diverse Environments In Phaseolus Vulgaris, Christopher F. Strock, James Burridge, Anica S.F. Massas, James S. Beaver, Steve E. Beebe, Samuel A. Camilo, Deidre Fourie, Celestina Nhagupana Jochua, Magalhaes Amade Miguel, Phillip N. Miklas, Eninka Mndolwa, Susan Nchimbi-Msolla, Jose Polania, T. Porch, Juan C. Rosas, Jennifer J. Trapp, Jonathan P. Lynch
Seedling Root Architecture And Its Relationship With Seed Yield Across Diverse Environments In Phaseolus Vulgaris, Christopher F. Strock, James Burridge, Anica S.F. Massas, James S. Beaver, Steve E. Beebe, Samuel A. Camilo, Deidre Fourie, Celestina Nhagupana Jochua, Magalhaes Amade Miguel, Phillip N. Miklas, Eninka Mndolwa, Susan Nchimbi-Msolla, Jose Polania, T. Porch, Juan C. Rosas, Jennifer J. Trapp, Jonathan P. Lynch
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Seedling root phenotypes may have important impacts on fitness and are more easily measured than mature root phenotypes. We phenotyped the roots of 577 genotypes of common bean (Phaseolus vulgaris), representing the bulk of the genetic diversity for recent cultivars and landraces in this species. Root architectural phenotypes of seedlings germinated for nine days were compared to root architectural phenotypes in the field as well as seed yield across 51 environments with an array of abiotic stresses including drought, nutrient deficiency, and heat, as well as non-stress conditions. We observed repeatability ranging from 0.52–0.57 for measures of root phenotypes in …
Phenotypes Of Macrophages Present In The Intestine Are Impacted By Stage Of Disease In Cattle Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Caitlin J. Jenvey, Adrienne L. Shircliff, J. P. Bannantine, Judith R. Stabel
Phenotypes Of Macrophages Present In The Intestine Are Impacted By Stage Of Disease In Cattle Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Caitlin J. Jenvey, Adrienne L. Shircliff, J. P. Bannantine, Judith R. Stabel
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Macrophages play an important role in the host immune response to Mycobacterium avium subsp. paratuberculosis (MAP) infection, however, MAP is able to disrupt normal macro- phage functions to avoid destruction. It is unclear whether the phenotypes of macrophages present in the target tissue play a role in the inability to clear MAP infection. The aim of this study was to identify macrophage phenotypes (host defense or resolution and repair) present within the bovine ileum of naturally infected cattle, as well as to ascertain abundance of each macrophage phenotype present during different stages of MAP infection. Immunofluo- rescent (IF) labeling was …
Effects Of Solids Removal On Water Quality And Channel Catfish Production In A Biofloc Technology Production System, Bartholomew W. Green, Kevin K. Schrader, Matthew Mcentire
Effects Of Solids Removal On Water Quality And Channel Catfish Production In A Biofloc Technology Production System, Bartholomew W. Green, Kevin K. Schrader, Matthew Mcentire
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Total suspended solids control was evaluated in a channel catfish (Ictalurus punctatus) biofloc technology production system. Settling chamber flow rates were 0.9 (LO) or 2.9 (HI) L/min to reduce total suspended solids to 300 mg/L; solids were not removed from control tanks. Channel catfish yields (7.6–8.7 kg/m3) were not affected significantly, but control fish were skewed toward smaller size classes. Control treatment channel catfish tolerated 1,410 mg/L total suspended solids without adverse effects. LO- and HI-treatment fillet geosmin concentrations were high enough to be designated as off-flavor. Water quality results suggested that nitrification was affected by solids removal.
Predicting Spatial Risk Of Wolf-Cattle Encounters On Rugged And Extensive Grazing Lands, Patrick R. Clark, Joe Chigbrow, Douglas E. Johnson, John Williams, Larry L. Larson, Tyanne Roland
Predicting Spatial Risk Of Wolf-Cattle Encounters On Rugged And Extensive Grazing Lands, Patrick R. Clark, Joe Chigbrow, Douglas E. Johnson, John Williams, Larry L. Larson, Tyanne Roland
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Cattle grazing lands in the mountainous western United States are rugged, complex, and extensive. Terrain, vegetation, and other landscape features vary greatly across space. Risk of wolf-cattle encounters and potential for depredation loss certainly differ spatially as consequence of this variability. Yet, our understanding of this spatial risk is quite poor and this knowledge gap severely hampers our abilities to manage wolf-livestock interactions and mitigate conflicts. During 2009-2011, a research study was conducted at four study areas (USFS cattle grazing allotments) in western Idaho to evaluate and predict risk of wolf-cattle encounters. Each year, a random sample of 10 lactating …
Quantification Of Macrophages And Mycobacterium Avium Subsp. Paratuberculosis In Bovine Intestinal Tissue During Different Stages Of Johne’S Disease, Caitlin J. Jenvey, Jesse M. Hostetter, Adrienne L. Shircliff, John Bannantine, Judith R. Stabel
Quantification Of Macrophages And Mycobacterium Avium Subsp. Paratuberculosis In Bovine Intestinal Tissue During Different Stages Of Johne’S Disease, Caitlin J. Jenvey, Jesse M. Hostetter, Adrienne L. Shircliff, John Bannantine, Judith R. Stabel
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Johne’s disease is an enteric disease caused by the intracellular pathogen Mycobacterium avium subsp. paratuberculosis (MAP). Upon ingestion of MAP, it is translocated across the intestinal epithelium and may be killed by intestinal macrophages, or depending on the bacterial burden and immunological status of the animal, MAP may thwart innate defense mechanisms and persist within the macrophage. This study aimed to determine the numbers of macrophages and MAP present in bovine midileal tissue during different stages of infection. Immunofluorescent (IF) labeling was performed on frozen bovine midileal intestinal tissue collected from 28 Holstein dairy cows. The number of macrophages in …
Identification Of Sero-Diagnostic Antigens For The Early Diagnosis Of Johne’S Disease Using Map Protein Microarrays, Lingling Li, J. P. Bannantine, Joseph J. Campo, Arlo Randall, Yrjo T. Grohn, Megan A. Schilling, Robab Katani, Jessica Radzio-Basu, Laurel Easterling, Vivek Kapur
Identification Of Sero-Diagnostic Antigens For The Early Diagnosis Of Johne’S Disease Using Map Protein Microarrays, Lingling Li, J. P. Bannantine, Joseph J. Campo, Arlo Randall, Yrjo T. Grohn, Megan A. Schilling, Robab Katani, Jessica Radzio-Basu, Laurel Easterling, Vivek Kapur
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Considerable effort has been directed toward controlling Johne’s disease (JD), a chronic granulomatous intestinal inflammatory disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) in cattle and other ruminants. However, progress in controlling the spread of MAP infection has been impeded by the lack of reliable diagnostic tests that can identify animals early in the infection process and help break the transmission chain. To identify reliable antigens for early diagnosis of MAP infection, we constructed a MAP protein array with 868 purified recombinant MAP proteins, and screened a total of 180 well-characterized serum samples from cows assigned to 4 groups based …
Characterization Of Ethanol Extracted Cell Wall Components Of Mycobacterium Avium Subsp. Paratuberculosis, John P. Bannantinea, Ashutosh Wadhwa, Judith R. Stabel, Shigetoshi Eda
Characterization Of Ethanol Extracted Cell Wall Components Of Mycobacterium Avium Subsp. Paratuberculosis, John P. Bannantinea, Ashutosh Wadhwa, Judith R. Stabel, Shigetoshi Eda
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Antigens extracted using ethanol (EtOH) and incorporated in the EtOH vortex ELISA (EVELISA) test have previously shown high specificity and sensitivity for detecting Mycobacterium avium subspecies paratuberculosis (Map) and M. bovis infections in cattle. The objective of this study is to define the components present in the EtOH extract. We show that this extract is composed of lipid, carbohydrate, and proteins on the surface of the bacilli, and that EtOH removes the outer layer structure of Map which comprise these elements. To identify proteins, polyclonal antibodies to the EtOH prep were produced and used to screen a Map genomic expression …
Irrigated Pinto Bean Crop Stress And Yield Assessment Using Ground Based Low Altitude Remote Sensing Technology, Rakesh Ranjan, Abhilash K. Chandel, Lav R. Khot, Haitham Y. Bahlol, Jianfeng Zhou, Rick A. Boydston, Phillip N. Miklas
Irrigated Pinto Bean Crop Stress And Yield Assessment Using Ground Based Low Altitude Remote Sensing Technology, Rakesh Ranjan, Abhilash K. Chandel, Lav R. Khot, Haitham Y. Bahlol, Jianfeng Zhou, Rick A. Boydston, Phillip N. Miklas
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The pinto bean is one of widely consumed legume crop that constitutes over 42% of the U.S dry bean production. However, limited studies have been conducted in past to assess its quantitative and qualitative yield potentials. Emerging remote sensing technologies can help in such assessment. Therefore, this study evaluates the role of ground-based multispectral imagery derived vegetation indices (VIs) for irrigated the pinto bean stress and yield assessments. Studied were eight cultivars of the pinto bean grown under conventional and strip tillage treatments and irrigated at 52% and 100% of required evapotranspiration. Imagery data was acquired using a five-band multispectral …
Gge Biplot Analysis Of Yield Stabi Lity For Andean Dry Bean Accessions Grown Under Different Abiotic Stress Regimes In Tanzania, E. J. Mndolwa, S. N. Msolla, T. G. Porch, Phillip N. Miklas
Gge Biplot Analysis Of Yield Stabi Lity For Andean Dry Bean Accessions Grown Under Different Abiotic Stress Regimes In Tanzania, E. J. Mndolwa, S. N. Msolla, T. G. Porch, Phillip N. Miklas
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Large seeded Andean dry beans (Phaseolus vulgaris L.) are most preferred in Africa. However, low soil fertility and increasing drought stress conditions due to climate change are among the challenges faced by farmers in this region. The purpose of this study was to identify Andean genotypes with yield stability and to identify the best environments for selection of this trait. GGE-Biplot analysis was used to examine 183 Andean bean genotypes in Tanzania. Results indicated significant environment (E), genotype (G) and genotype x environment (GE) effects for seed yield. The environment explained 46%, genotype 20%, and GE 34% of the total …
Generation And Validation Of Genetic Markers For The Selection Of Carioca Dry Bean Genotypes With The Slow-Darkening Seed Coat Trait, Renata C. Alvares, Robert Stonehouse, Thiago L.P.O. Souza, Patrícia G.S. Melo, Phillip N. Miklas, Kirstin E. Bett, Leonardo C. Melo, Luana A. Rodrigues, Lorena L. Souza, Helton S. Pereira
Generation And Validation Of Genetic Markers For The Selection Of Carioca Dry Bean Genotypes With The Slow-Darkening Seed Coat Trait, Renata C. Alvares, Robert Stonehouse, Thiago L.P.O. Souza, Patrícia G.S. Melo, Phillip N. Miklas, Kirstin E. Bett, Leonardo C. Melo, Luana A. Rodrigues, Lorena L. Souza, Helton S. Pereira
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Slow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepe´rola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Nota´vel and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd- 1158 and PVM02TC116 and with a …
Divergent Antigen-Specific Cellular Immune Responses During Asymptomatic Subclinical And Clinical States Of Disease In Cows Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Judith R. Stabel, J. P. Bannantine
Divergent Antigen-Specific Cellular Immune Responses During Asymptomatic Subclinical And Clinical States Of Disease In Cows Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Judith R. Stabel, J. P. Bannantine
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Infection of the host with Mycobacterium avium subsp. paratuberculosis results in chronic and progressive enteritis that traverses both subclinical and clinical stages. The mechanism(s) for the shift from an asymptomatic subclinical disease state to advanced clinical disease is not fully understood. In the present study, naturally infected dairy cattle were divided into subclinical and clinical infection groups, along with noninfected control cows of similar parity, to study host immune responses in different stages of infection. Both infection groups had higher levels of secretion of gamma interferon (IFN-), tumor necrosis factor-alpha (TNF-), and interleukin-2 (IL-2) than control cows, whereas only clinical …
Capacity To Elicit Cytotoxic Cd8 T Cell Activity Against Mycobacterium Avium Subsp. Paratuberculosis Is Retained In A Vaccine Candidate 35 Kda Peptide Modified For Expression In Mammalian Cells, Valentina Franceschi, Asmaa H. Mahmoud, Gaber S. Abdellrazeq, Giulia Tebaldi, Francesca Macchi, Luca Russo, Lindsay M. Fry, Mahmoud M. Elnaggar, John Bannantine, Kun-Taek Park, Victoria Hulubei, Sandro Cavirani, William C. Davis, Gaetano Donofrio
Capacity To Elicit Cytotoxic Cd8 T Cell Activity Against Mycobacterium Avium Subsp. Paratuberculosis Is Retained In A Vaccine Candidate 35 Kda Peptide Modified For Expression In Mammalian Cells, Valentina Franceschi, Asmaa H. Mahmoud, Gaber S. Abdellrazeq, Giulia Tebaldi, Francesca Macchi, Luca Russo, Lindsay M. Fry, Mahmoud M. Elnaggar, John Bannantine, Kun-Taek Park, Victoria Hulubei, Sandro Cavirani, William C. Davis, Gaetano Donofrio
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Studies focused on development of an attenuated vaccine against Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of paratuberculosis (Ptb) in cattle and other species, revealed that deletion of relA, a global gene regulator, abrogates the ability of Map to establish a persistent infection. In the absence of relA, cattle develop CD8 cytotoxic T cells (CTL) with the ability to kill intracellular bacteria. Analysis of the recall response to a relA mutant, Map/relA, with cells from a vaccinated steer demonstrated that a 35-kDa membrane peptide (MMP) is one of the targets of the response. This observation suggested that it might …
The Role Of Genotype And Production Environment In Determining The Cooking Time Of Dry Beans (Phaseolus Vulgaris L.), Karen A. Cichy, Jason A. Wiesinger, Matthew Berry, Susan Nchimbi‐Msolla, Deidre Fourie, Timothy G. Perch, Daniel Ambechew, Phillip N. Miklas
The Role Of Genotype And Production Environment In Determining The Cooking Time Of Dry Beans (Phaseolus Vulgaris L.), Karen A. Cichy, Jason A. Wiesinger, Matthew Berry, Susan Nchimbi‐Msolla, Deidre Fourie, Timothy G. Perch, Daniel Ambechew, Phillip N. Miklas
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Dry bean (Phaseolus vulgaris L.) is a nutrient‐dense food rich in proteins and minerals. Although a dietary staple in numerous regions, including Eastern and Southern Africa, greater utilization is limited by its long cooking time as compared with other staple foods. A fivefold genetic variability for cooking time has been identified for P. vulgaris, and to effectively incorporate the cooking time trait into bean breeding programs, knowledge of how genotypes behave across diverse environments is essential. Fourteen bean genotypes selected from market classes important to global consumers (yellow, cranberry, light red kidney, red mottled, and brown) were grown …
A Peptide-Based Vaccine For Mycobacterium Avium Subspecies Paratuberculosis, Gaber S. Abdellrazeq, Mahmoud M. Elnaggar, J. P. Bannantine, David A. Schneider, Cleverson D. Souza, Julianne Hwang, Asmaa H.A. Mahmoud, Victoria Hulubei, Lindsay M. Fry, Kun-Taek Park, William C. Davis
A Peptide-Based Vaccine For Mycobacterium Avium Subspecies Paratuberculosis, Gaber S. Abdellrazeq, Mahmoud M. Elnaggar, J. P. Bannantine, David A. Schneider, Cleverson D. Souza, Julianne Hwang, Asmaa H.A. Mahmoud, Victoria Hulubei, Lindsay M. Fry, Kun-Taek Park, William C. Davis
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Recent efforts to develop a live attenuated vaccine against Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of Johne’s disease (JD), revealed relA is important in Map virulence. Deletion of the relA gene impairs the ability of Map to establish a persistent infection. Analysis of the basis for this observation revealed infection with a relA deletion mutant (DrelA) elicits development of cytotoxic CD8 T cells (CTL) with the ability to kill intracellular bacteria. Further analysis of the recall response elicited by DrelA vaccination showed a 35 kDa membrane peptide (MMP) is one of the targets of the immune response, suggesting …
Wolves: A Primer For Ranchers, John Williams, Douglas E. Johnson, Patrick E. Clark, Larry L. Larson, Tyanne J. Roland
Wolves: A Primer For Ranchers, John Williams, Douglas E. Johnson, Patrick E. Clark, Larry L. Larson, Tyanne J. Roland
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Ranch management has become more complex since wolves were reintroduced into Idaho and Wyoming in 1996. In wolf areas, livestock have experienced increased death loss and greater stress. Increased livestock aggressiveness has been observed, especially toward working dogs, making handling livestock more difficult. Additionally ranchers have reported a loss of body condition, lower conception rates, increased time and expense for management. Our study was designed to investigate the effect of wolf presence on cattle behavior, landscape use patterns, and resource selection by comparing high wolf density areas against low wolf density areas. This study also generated baseline information on cattle …