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Hplc Analysis Of Flavonoids In Apocynumvenetum Tea And Its Antioxidation In Vitro, Zhang Jing, Ren Xiaoli, Li Xixi, Yi Sha, Li Chong, Zhao Xin
Hplc Analysis Of Flavonoids In Apocynumvenetum Tea And Its Antioxidation In Vitro, Zhang Jing, Ren Xiaoli, Li Xixi, Yi Sha, Li Chong, Zhao Xin
Food and Machinery
In this study, the contents of rutin and isoquercetin in flavonoids of Apocynumvenetum tea was determined by high performance liquid chromatography (HPLC), and the antioxidant effect in vitro was evaluated. A good separation of rutin and isoquercetin from flavonoids of Apocynumvenetum tea was achieved by vacuum heating reflux combined with macroporous adsorption. Rutin and isoquercetin were separated effectively in the extract of Apocynum venetum teaflavonoids. The results showed good linear relationship, the range of rutin was 0.453 1~9.062 5 μg (r=0.999 55) and that of isoquercetin ranged from 0.258 3 to 5.166 5 μg (r=0.999 65). The recoveries of rutin …