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Full-Text Articles in Agriculture
Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot
Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot
Department of Agronomy and Horticulture: Faculty Publications
Large DNA insert libraries in binary T-DNA vectors can assist in the isolation of the gene(s) under-lying a quantitative trait locus (QTL). Binary vectors facilitate the transfer of large-insert DNA fragments containing a QTL from E. coli to Agrobacterium sp. and then to plants. We constructed two soybean large-insert libraries from cv. Forrest in the pCLD04541 (V41) binary vector after partial digestion of genomic high-molecular-weight DNA with BamHI or HindIII. The libraries contain 76,800 clones with an average insert size of 125 kb, and therefore represent 9.5-fold haploid genome equivalents. Colony hybridization using a chloroplast-specific probe infers that …
Before You Say Yes: A Planning Guide For Speakers, Charles A. Francis, Heidi Carter, Cris Carusi, James W. King
Before You Say Yes: A Planning Guide For Speakers, Charles A. Francis, Heidi Carter, Cris Carusi, James W. King
Department of Agronomy and Horticulture: Faculty Publications
We need guidelines to help us decide whether to accept invitations to speak, whether to a class on campus or a special interest group outside. As educators and workshop organizers, we could also use suggestions on how to approach potential speakers. This article describes a single-page format that can be used to guide the planning process. Essential elements include contact information, location and organization of the activity, audience, learning goals, expected content, conclusions, and evaluation. Use of this planning sheet can give organization to an often haphazard process of planning, and enhance the potential of achieving the learning goals of …
Crop Quality And Utilization: A Twelve-Hour In Vitro Procedure For Sorghum Grain Feed Quality Assessment, Jeffrey F. Pedersen, Todd Milton, R. A. Mass
Crop Quality And Utilization: A Twelve-Hour In Vitro Procedure For Sorghum Grain Feed Quality Assessment, Jeffrey F. Pedersen, Todd Milton, R. A. Mass
Department of Agronomy and Horticulture: Faculty Publications
Improvedmethods for assessing cereal crop feed value are a prerequisite for the genetic improvement of sorghum [Sorghum bicolor (L.) Moench] feed value. Rate of starch digestion is now commonly believed to be the limiting factor in sorghum utilization by cattle (Bos taurus). However, techniques to assess this trait are not useful to sorghum breeders because of high labor inputs, lab error associated with starch measurement, and need for high numbers of replications. The objective of this study was to develop a simple technique capable of identifying differences in digestion between sorghum and corn (Zea mays L.) …
Ethylene Production, Cluster Root Formation, And Localization Of Iron(Iii) Reducing Capacity In Fe Deficient Squash Roots, Brian M. Waters, Dale G. Blevins
Ethylene Production, Cluster Root Formation, And Localization Of Iron(Iii) Reducing Capacity In Fe Deficient Squash Roots, Brian M. Waters, Dale G. Blevins
Department of Agronomy and Horticulture: Faculty Publications
Dicots and non-graminaceous monocots have the ability to increase root iron(III) reducing capacity in response to iron (Fe) deficiency stress. In squash (Cucurbita pepo L.) seedlings, Fe(III) reducing capacity was quantified during early vegetative growth. When plants were grown in Fe-free solution, the Fe(III) reducing capacity was greatly elevated, reached peak activity on day 4, then declined through day 6. Root ethylene production exhibited a temporal pattern that closely matched that of Fe(III) reducing capacity through day 6. On the 7th day of Fe deficiency, cluster root morphology developed, which coincided with a sharp increase in the root Fe(III) …