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Full-Text Articles in Agriculture

The Development Of An Efficient Multipurpose Bean Pod Mottle Virus Viral Vector Set For Foreign Gene Expression And Rna Silencing, Chunquan Zhang, Jeffrey Bradshaw, Steven A. Whitham, John H. Hill Jan 2010

The Development Of An Efficient Multipurpose Bean Pod Mottle Virus Viral Vector Set For Foreign Gene Expression And Rna Silencing, Chunquan Zhang, Jeffrey Bradshaw, Steven A. Whitham, John H. Hill

Panhandle Research and Extension Center

Plant viral vectors are valuable tools for heterologous gene expression, and because of virus-induced gene silencing (VIGS), they also have important applications as reverse genetics tools for gene function studies. Viral vectors are especially useful for plants such as soybean (Glycine max) that are recalcitrant to transformation. Previously, two generations of bean pod mottle virus (BPMV; genus Comovirus) vectors have been developed for overexpressing and silencing genes in soybean. However, the design of the previous vectors imposes constraints that limit their utility. For example, VIGS target sequences must be expressed as fusion proteins in the same reading …


Symptoms, Distribution And Abundance Of The Stem-Boring Caterpillar, Blastobasis Repartella (Dietz), In Switchgrass, Jarrad R. Prasifka, Jeffrey Bradshaw, Arvid A. Boe, Dokyoung Lee, David Adamski, Michael E. Gray Jan 2010

Symptoms, Distribution And Abundance Of The Stem-Boring Caterpillar, Blastobasis Repartella (Dietz), In Switchgrass, Jarrad R. Prasifka, Jeffrey Bradshaw, Arvid A. Boe, Dokyoung Lee, David Adamski, Michael E. Gray

Panhandle Research and Extension Center

A potential pest of switchgrass, Panicum virgatum L., was first detected in South Dakota in 2004, where death of partially emerged leaves was noted in a small proportion of tillers. Similar “dead heart” symptoms were observed in switchgrass in Illinois during 2008 and adults of a stem-boring caterpillar were collected and identified as Blastobasis repartella (Dietz). In 2009, a survey of the central United States was used to estimate the distribution and abundance of this insect. In eight northern states, B. repartella was consistently found in both cultivated plots and natural stands of switchgrass. In four southern states, B. repartella …


First Report Of Field Populations Of Two Potential Aphid Pests Of The Bioenergy Crop Miscanthus × Giganteus, Jeffrey Bradshaw, Jarrad R. Prasifka, Kevin L. Steffey, Michael E. Gray Jan 2010

First Report Of Field Populations Of Two Potential Aphid Pests Of The Bioenergy Crop Miscanthus × Giganteus, Jeffrey Bradshaw, Jarrad R. Prasifka, Kevin L. Steffey, Michael E. Gray

Panhandle Research and Extension Center

Sipha flava (Forbes) (yellow sugarcane aphid) and Rhopalosiphum maidis (Fitch) (corn leaf aphid) (Hemiptera: Homoptera: Aphididae) are common aphids occurring throughout North America on many host plants, most of which are grasses (Blackman & Eastop 2006). Both aphids are pests of several important food crops, e.g., Sorghum bicolor (L.) Moench (sorghum), Saccharum officinarum L. (sugarcane), Triticum spp. (wheat), and Zea mays L. (corn) (Blackman & Eastop 2000). Additionally, both aphids are vectors of potyviruses and R. maidis is a vector of luteoviruses in these crops. Until now, to our knowledge, no natural infestations of these aphids have been reported on …


Descriptions Of Life-Stages Of Blastobasis Repartella (Lepidoptera: Gelechioidea: Coleophoridae: Blastobasinae) And Observations On Its Biology In Switchgrass, D. Adamski, P. J. Johnson, A. A. Boe, Jeffrey Bradshaw, Alan Pultyniewicz Jan 2010

Descriptions Of Life-Stages Of Blastobasis Repartella (Lepidoptera: Gelechioidea: Coleophoridae: Blastobasinae) And Observations On Its Biology In Switchgrass, D. Adamski, P. J. Johnson, A. A. Boe, Jeffrey Bradshaw, Alan Pultyniewicz

Panhandle Research and Extension Center

Blastobasis repartella (Dietz) is a borer in the proaxis and basal nodes and internodes of above ground stems of Panicum virgatum L. (Poaceae). The adult and immature stages are described herein, including diagnoses of the adult and larva, as this insect may be easily confused with a closely related grass-feeding congener, Blastobasis graminea Adamski, which is also known to occur in the United States. The biology of B. repartella is described. Figures of the adult, illustrations of the male and female genitalia, wing venation, the chaetotaxy of the larva (supplemented with scanning electron micrographs), and pupa are provided. Bassus difficilis …