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Development Of Serological Procedures For Rapid And Reliable Detection Of Wheat Streak Mosaic Virus In A Single Wheat Curl Mite, T. Mahmood, Gary L. Hein, Roy C. French
Development Of Serological Procedures For Rapid And Reliable Detection Of Wheat Streak Mosaic Virus In A Single Wheat Curl Mite, T. Mahmood, Gary L. Hein, Roy C. French
Panhandle Research and Extension Center
Wheat streak mosaic virus (WSMV) is transmitted by the wheat curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed to detect the presence of WSMV in single WCM. Virus-specific immunofluorescent microscopy detected WSMV near the anterior end of viruliferous WCM. With dot-immunobinding assay, WSMV was detected in WCM fed on WSMV-infected wheat (Triticum aestivum) but not in WCM maintained on healthy plants. Both immunofluorescent and dot-immunobinding assays were sufficiently sensitive to detect WSMV in individual WCM, providing a means to determine the percentage of viruliferous WCM in field collections.