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TÜBİTAK

2009

PCR

Articles 1 - 2 of 2

Full-Text Articles in Agriculture

Molecular Characterization And Genetic Diversity Analysis Of Citrus Cultivars By Rapd Markers, M. N. R. Baig, Sapna Grewal, Santosh Dhillon Jan 2009

Molecular Characterization And Genetic Diversity Analysis Of Citrus Cultivars By Rapd Markers, M. N. R. Baig, Sapna Grewal, Santosh Dhillon

Turkish Journal of Agriculture and Forestry

Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers; of which 231 were polymorphic and some species or cultivar specific RAPD markers. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated Jatti-Khatti from all major clusters at a similarity coefficient of 0.61. The average genetic similarity value observed across all the genotypes was 0.63, with the 2 sweet orange cultivars, Jaffa …


Putative Agents Of Fig Mosaic Disease In Turkey, Kadri̇ye Çağlayan, Vicente Medina, Mona Gazel, Çi̇ğdem U. Serçe, Luis Serrano, Angeles Achon, Soner Soylu, Oğuzhan Çalişkan, Mustafa Gümüş Jan 2009

Putative Agents Of Fig Mosaic Disease In Turkey, Kadri̇ye Çağlayan, Vicente Medina, Mona Gazel, Çi̇ğdem U. Serçe, Luis Serrano, Angeles Achon, Soner Soylu, Oğuzhan Çalişkan, Mustafa Gümüş

Turkish Journal of Agriculture and Forestry

Leaves from 58 fig tree accessions showing different severity of mosaic and necrotic symptoms were collected in 2 different regions of Turkey and analysed by transmission electron microscope (TEM) and RT-PCR to determine putative causal agents of fig mosaic disease (FMD). Ultrastructural studies showed that double membrane-bound bodies (DMBs) were mainly observed in the parenchyma and mesophyll cells of infected leaves of the cultivars Göklop and Morgüz and some seedlings showing only mosaic symptoms. These bodies were surrounded by a fibrillar matrix in most of the infected cells. Long, flexuous rod-shaped virus-like particles (LFPs) were also observed in vascular tissues …