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Full-Text Articles in Agriculture
Effect Of Cry1f Maize On The Behavior Of Susceptible And Resistant Spodoptera Frugiperda And Ostrinia Nubilalis, Ana María Vélez, Analiza P. Alves, Erin E. Blankenship, Blair Siegfried
Effect Of Cry1f Maize On The Behavior Of Susceptible And Resistant Spodoptera Frugiperda And Ostrinia Nubilalis, Ana María Vélez, Analiza P. Alves, Erin E. Blankenship, Blair Siegfried
Department of Entomology: Faculty Publications
Understanding the behavior of pests targeted with Bacillus thuringiensis Berliner (Bt) crops is important to define resistance management strategies. Particularly the study of larval movement between plants is important to determine the feasibility of refuge configurations. Exposure to Bt maize, Zea mays L. (Poaceae), has been suggested to increase larval movement in lepidopteran species but few studies have examined the potential for resistance to interact with behavioral responses to Bt toxins. Choice and no-choice experiments were conducted with Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) and Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) to determine whether Cry1F resistance influences neonate movement. Leaf discs …
Characterization Of Resistance To The Cry1f Toxin From Bacillus Thuringiensis In Resistant Fall Armyworm, Spodoptera Frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) From Puerto Rico, Ana Maria Velez
Department of Entomology: Dissertations, Theses, and Student Research
Transgenic corn expressing Cry1F protein from Bacillus thuringiensis Berliner has been registered for Spodoptera frugiperda control since 2003. Unexpected damage to Cry1F corn was reported in 2006 in Puerto Rico and Cry1F resistance in S. frugiperda was documented. The inheritance of Cry1F resistance was characterized in a S. frugiperda resistant strain from Puerto Rico which displayed >387-fold resistance to Cry1F. Concentration-response bioassays of reciprocal crosses of resistant and susceptible parental populations indicated that resistance is recessive and autosomal. Bioassays of the backcross of the F1 generation crossed with the resistant parental strain suggest that a single locus is responsible …
Mortality Impact Of Mon863 Transgenic Maize Roots On Western Corn Rootworm Larvae In The Field, T. L. Clark, D. L. Frank, B. W. French, Lance J. Meinke, D. Moellenbeck, T. T. Vaughn, B. E. Hibbard
Mortality Impact Of Mon863 Transgenic Maize Roots On Western Corn Rootworm Larvae In The Field, T. L. Clark, D. L. Frank, B. W. French, Lance J. Meinke, D. Moellenbeck, T. T. Vaughn, B. E. Hibbard
Department of Entomology: Faculty Publications
Mortality of western corn rootworm (Diabrotica virgifera virgifera LeConte) due to feeding on MON863 transgenic maize (Zea mays L.) expressing the Cry3Bb1 protein was evaluated at three Missouri sites in both 2003 and 2004 and at one site each in South Dakota, Nebraska, and Iowa in 2004. To do this, survivorship relative to survivorship on isoline maize (i.e., the same genetic background, but without Cry3Bb1) was evaluated. Comparisons were made using low (1650–2500 eggs/m) and high (3300–3500 eggs/m) western corn rootworm egg densities. Significantly fewer beetles were recovered from MON863 than from isoline maize. Emergence from MON863 as …
Larval Survival And Plant Injury Of Cry1ab-Susceptible, -Resistant, And -Heterozygous Genotypes Of The Sugarcane Borer On Transgenic Corn Containing Single Or Pyramided Bt Genes, David S. Wangila, B. Rogers Leonard, Yaoyu Bai, Graham P. Head, Fangneng Huang
Larval Survival And Plant Injury Of Cry1ab-Susceptible, -Resistant, And -Heterozygous Genotypes Of The Sugarcane Borer On Transgenic Corn Containing Single Or Pyramided Bt Genes, David S. Wangila, B. Rogers Leonard, Yaoyu Bai, Graham P. Head, Fangneng Huang
Department of Entomology: Faculty Publications
Transgenic corn (Zea mays L.) products expressing multiple Bt proteins targeting a same group of insect pests have become commercially available in the U.S. The first commercialized pyramided Bt corn technologies for managing lepidopteran pests include Genuity® VT Triple Pro™ and Genuity® SmartStax™. In this study, larval survival and plant injury of Cry1Ab-susceptible (Cry1Ab-SS), -resistant (Cry1Ab-RR), and -heterozygous (Cry1Ab-RS) genotypes of the sugarcane borer, Diatraea saccharalis F., on five commercial corn hybrids were evaluated in a leaf tissue bioassay and two greenhouse trials during 2010–2011. The five hybrids included two non-Bt corn and three Bt corn hybrids representing three …
Susceptibility Of Cry1ab Maize-Resistant And -Susceptible Strains Of Sugarcane Borer (Lepidoptera: Crambidae) To Four Individual Cry Proteins, Liping Zhang, Fangneng Huang, B. Rogers Leonard, Mao Chen, Thomas Clark, Yu Cheng Zhu, David S. Wangila, Fei Yang, Ying Niu
Susceptibility Of Cry1ab Maize-Resistant And -Susceptible Strains Of Sugarcane Borer (Lepidoptera: Crambidae) To Four Individual Cry Proteins, Liping Zhang, Fangneng Huang, B. Rogers Leonard, Mao Chen, Thomas Clark, Yu Cheng Zhu, David S. Wangila, Fei Yang, Ying Niu
Department of Entomology: Faculty Publications
Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F2 families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac, and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) …
Pollen Interception By Linyphiid Spiders In A Corn Agroecosystem: Implications For Dietary Diversification And Risk-Assessment, Julie A. Peterson, Susan Romero, James D. Harwood
Pollen Interception By Linyphiid Spiders In A Corn Agroecosystem: Implications For Dietary Diversification And Risk-Assessment, Julie A. Peterson, Susan Romero, James D. Harwood
West Central Research and Extension Center, North Platte
Dietary diversification, including consumption of plant tissues such as pollen, can enhance the fecundity of generalist predators, resulting in improved control of pest prey. Supplemental pollen feeding has been observed in many natural enemies, including sheet-web spiders (Araneae: Linyphiidae), which represent a major component of food webs in agroecosystems. Their horizontal, ground-based webs have the potential to intercept pollen grains during anthesis of crop plants, providing the opportunity for consumption of pollen to occur. In laboratory feeding trials, Frontinella communis and Tennesseellum formicum (Araneae: Linyphiidae) readily fed on pollen grains dusted on their webs, with 82 and 92% of spiders …
Quantification Of Bt-Endotoxin Exposure Pathways In Carabid Food Webs Across Multiple Transgenic Events, Julie A. Peterson, John J. Obrycki, James D. Harwood
Quantification Of Bt-Endotoxin Exposure Pathways In Carabid Food Webs Across Multiple Transgenic Events, Julie A. Peterson, John J. Obrycki, James D. Harwood
West Central Research and Extension Center, North Platte
Despite the reported specificity of Bacillus thuringiensis proteins against target pests, a number of studies have indicated that the uptake of Bt-endotoxins from bioengineered crops could have negative effects on natural enemies. It is therefore essential to quantify exposure pathways in non-target arthropod food webs across multiple transgenic events. Adult ground beetles (Coleoptera: Carabidae) were collected from transgenic corn fields expressing lepidopteran-specific Cry1Ab, coleopteran-specific Cry3Bb1, and both Cry1Ab and Cry3Bb1 (stacked event), as well as a non-transgenic isoline. Carabid gut-contents were screened for Cry1Ab Bt-endotoxin using enzyme-linked immunosorbent assay. Significant numbers of carabids tested positive for Cry1Ab from the lepidopteran-specific …
Oviposition By European Corn Borer (Lepidoptera: Crambidae) In Response To Various Transgenic Corn Events, Richard L. Hellmich, L. S. Higgins, John F. Witkowski, J. E. Campbell, L. C. Lewis
Oviposition By European Corn Borer (Lepidoptera: Crambidae) In Response To Various Transgenic Corn Events, Richard L. Hellmich, L. S. Higgins, John F. Witkowski, J. E. Campbell, L. C. Lewis
Haskell Agricultural Laboratory (Northeast Research and Extension Center)
Oviposition preference by European corn borers, Ostrinia nubilalis (Hübner), for or against transgenic corn would influence amounts of refuge required for resistance management. The objective of this research was to determine if various Bacillus thuringiensis (Bt) corn transgenic events influence O. nubilalis oviposition. All commercially available events (currently 5) were evaluated, plus 1 experimental event. Results from 3 independent studies are reported, including 3 field-cage experiments with vegetative corn, 2 field-cage experiments with reproductive corn, and 2 field experiments with natural O. nubilalis on reproductive corn. In each case, Bt corn hybrids are compared with their near isogenic hybrids by …