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Articles 1 - 25 of 25
Full-Text Articles in Life Sciences
Agro-Ecological Effects Of Sewage Sludge Application To Orchard Grass (Dactylis Glomerata) Fields, G. E. Merzlaya, R. A. Afanasiev, M. E. Shibaeva, I. A. Arkhipchenko
Agro-Ecological Effects Of Sewage Sludge Application To Orchard Grass (Dactylis Glomerata) Fields, G. E. Merzlaya, R. A. Afanasiev, M. E. Shibaeva, I. A. Arkhipchenko
IGC Proceedings (1997-2023)
Municipal sewage sludge can have a great fertilisation effect. However, its application is limited by high amounts of heavy metals, which have adverse effects on soil biota and plants (Ladonin et al., 2002). Our work was aimed at investigating the sludge effect on a soil-plant system.
Extracellular Dnases Facilitate Antagonism And Coexistence In Bacterial Competitor-Sensing Interference Competition, Aoi Ogawa, Christophe Golé, Maria Bermudez, Odrine Habarugira, Gabrielle Joslin, Taylor Mccain, Autumn Mineo, Jennifer Wise, Julie Xiong, Katherine Yan, Jan A.C. Vriezen
Extracellular Dnases Facilitate Antagonism And Coexistence In Bacterial Competitor-Sensing Interference Competition, Aoi Ogawa, Christophe Golé, Maria Bermudez, Odrine Habarugira, Gabrielle Joslin, Taylor Mccain, Autumn Mineo, Jennifer Wise, Julie Xiong, Katherine Yan, Jan A.C. Vriezen
Biological Sciences: Faculty Publications
Over the last 4 decades, the rate of discovery of novel antibiotics has decreased drastically, ending the era of fortuitous antibiotic discovery. A better understanding of the biology of bacteriogenic toxins potentially helps to prospect for new antibiotics. To initiate this line of research, we quantified antagonists from two different sites at two different depths of soil and found the relative number of antagonists to correlate with the bacterial load and carbon-to-nitrogen (C/N) ratio of the soil. Consecutive studies show the importance of antagonist interactions between soil isolates and the lack of a predicted role for nutrient availability and, therefore, …
Effect Of Soil Chemistry On Microbial Biodiversity And Functionality In Grassland And Tilled Soils, C. Carrigg, S. Kavanagh, D. Fay, V. O. Flaherty
Effect Of Soil Chemistry On Microbial Biodiversity And Functionality In Grassland And Tilled Soils, C. Carrigg, S. Kavanagh, D. Fay, V. O. Flaherty
IGC Proceedings (1997-2023)
Microorganisms are excellent indicators of soil health, because of their rapid response to environmental change. Traditional microbiology is ineffective for the study of soil, as < 1% of microorganisms are currently culturable (Torsvik et al., 1996). Nucleic acid based methods, however, allow rapid detection of organisms, or particular genes, directly from soil samples. This work investigated, using polymerase chain reaction (PCR)-based approaches, the relationship between key chemical properties and bacterial biodiversity in grassland and tilled soils, with particular emphasis on the abundance and diversity of ammonium oxidisers.
Microbial Labilization And Diversification Of Pyrogenic Dissolved Organic Matter, Aleksandar I. Goranov, Andrew S. Wozniak, Kyle W. Bostick, Andrew R. Zimmerman, Siddhartha Mitra, Patrick G. Hatcher
Microbial Labilization And Diversification Of Pyrogenic Dissolved Organic Matter, Aleksandar I. Goranov, Andrew S. Wozniak, Kyle W. Bostick, Andrew R. Zimmerman, Siddhartha Mitra, Patrick G. Hatcher
Chemistry & Biochemistry Faculty Publications
With the increased occurrence of wildfires around the world, interest in the chemistry of pyrogenic organic matter (pyOM) and its fate in the environment has increased. Upon leaching from soils by rain events, significant amounts of dissolved pyOM (pyDOM) enter the aquatic environment and interact with microbial communities that are essential for cycling organic matter within the different biogeochemical cycles. To evaluate the biodegradability of pyDOM, aqueous extracts of laboratory-produced biochars were incubated with soil microbes, and the molecular changes to the composition of pyDOM were probed using ultrahigh-resolution mass spectrometry (Fourier transform–ion cyclotron resonance–mass spectrometry). Given that solar irradiation …
Impacts Of Soil Management On Microbial Assemblages Involved In Nitrogen Transformations In Agroecosystems In Tennessee, Usa, Jialin Hu
Doctoral Dissertations
Nutrient reduction, particularly with respect to nitrogen (N) losses, is an important goal for sustainably managed agroecosystems. Soil N-cycling microbial populations that modulate these processes are affected by agricultural management regimes. This research focused on the controls and dynamics of the major N-cycling microbial populations in high-input cotton field under agricultural management regimes and low-input native C4 forage grass systems under pasture management practices to determine the effects of management regimes on in situ seasonal dynamics of the functional microbes responsible for N fixation, nitrification, and denitrification processes. Molecular microbial ecology methods were combined with soil physicochemical properties and …
Evaluation Of Cool-Season Annuals Effect On Soil Health In Warm-Season Perennial Pastures In Southestern Us., Paola C. Muela Negrete
Evaluation Of Cool-Season Annuals Effect On Soil Health In Warm-Season Perennial Pastures In Southestern Us., Paola C. Muela Negrete
LSU Master's Theses
Perennial grass crops represent approximately 8 million hectares of the land area of the humid lower southeastern United States. These forage crops receive high rates of fertilizer, especially nitrogen (N), and near monoculture remains have often been treated with repeated applications of herbicides. Pasture management is crucial to improve soil properties in pasturelands. Common pasture management practices include introducing cool-season multispecies in warm-season pasture systems and forage harvest frequency of pasture systems. It is known that cool-season multispecies in warm-season pasture systems ensure cattle feeding during winter season and have beneficial effects on soil microbial biomass, soil organic matter (SOM), …
The Effect Of Fallow Season Cover Crops On Nutrient Cycling And Soil Health In Row Crop Production In The Mid-South, Kritsanee Iamjud
The Effect Of Fallow Season Cover Crops On Nutrient Cycling And Soil Health In Row Crop Production In The Mid-South, Kritsanee Iamjud
LSU Doctoral Dissertations
The reliance on conventional cropping has profoundly impacted agricultural sustainability and resulted in soil degradation. Winter cover crops can preserve the soil surface, recycle soil nutrients, and improve soil health. Hence, the role of cover crops in Louisiana row-crop production was examined in small-scale plots and large-scale on-farm studies at three sites in Northeast Louisiana from 2017 to 2020. Additionally, mixed cover crops degradation and nutrient release were evaluated at Macon Ridge and Dean Lee sites to determine the optimum time of nutrient availability after cover crop termination. The small-scale results showed that integrating cover crops for corn (Zea …
Soil Health Management Enhances Microbial Nitrogen Cycling Capacity And Activity, Jianlin Hu, Virginia L. Jin, Julie Y.M. Konkel, Sean M. Schaeffer, Liesel G. Schneider, Jennifer M. Debruyn
Soil Health Management Enhances Microbial Nitrogen Cycling Capacity And Activity, Jianlin Hu, Virginia L. Jin, Julie Y.M. Konkel, Sean M. Schaeffer, Liesel G. Schneider, Jennifer M. Debruyn
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Soil microbial transformations of nitrogen (N) can be affected by soil health management practices. Here, we report in situ seasonal dynamics of the population size (gene copy abundances) and functional activity (transcript copy abundances) of five bacterial genes involved in soil N cycling (ammonia-oxidizing bacteria [AOB] amoA, nifH, nirK, nirS, and nosZ) in a long-term continuous cotton production system under different management practices (cover crops, tillage, and inorganic N fertilization). Hairy vetch (Vicia villosa Roth), a leguminous cover crop, most effectively promoted the expression of N cycle genes, which persisted after cover crop termination throughout the growing season. Moreover, we …
Biolability Of Fresh And Photodegraded Pyrogenic Dissolved Organic Matter From Laboratory-Prepared Chars, K. W. Bostick, A. R. Zimmerman, A. I. Goranov, S. Mitra, P. G. Hatcher, A. S. Wozniak
Biolability Of Fresh And Photodegraded Pyrogenic Dissolved Organic Matter From Laboratory-Prepared Chars, K. W. Bostick, A. R. Zimmerman, A. I. Goranov, S. Mitra, P. G. Hatcher, A. S. Wozniak
Chemistry & Biochemistry Faculty Publications
Pyrogenic dissolved organic matter (pyDOM) is known to be an important biogeochemical constituent of aquatic ecosystems and the carbon cycle. While recent studies have examined how pyDOM production, composition, and photolability varies with parent pyrogenic solid material type, we lack an understanding of potential microbial mineralization and transformation of pyDOM in the biogeosphere. Thus, leachates of oak, charred at 400 °C and 650 °C, as well as their photodegraded counterparts were incubated with a soil‐extracted microbial consortium over 96 days. During the incubation, significantly more carbon was biomineralized from the lower versus higher temperature char leachate (45% vs. 37% lost, …
Massard Prairie Restoration And Soil Microbiome Succession, Jeffrey M. Shaver, Emily S. Bellis, Chizuko Iwaki, Jake Qualls, Jay Randolph, Jeremiah Smith
Massard Prairie Restoration And Soil Microbiome Succession, Jeffrey M. Shaver, Emily S. Bellis, Chizuko Iwaki, Jake Qualls, Jay Randolph, Jeremiah Smith
Journal of the Arkansas Academy of Science
We have initially sequenced soil microbial DNA from 4 restored and 4 virgin tallgrass prairie soil samples from Ben Geren Park and Massard Prairie (Fort Smith, AR), respectively. As expected, the soil microbiomes are distinct, with several lineages of nitrogen-fixing bacteria more common in virgin tall grass prairie. However, we predict that as restoration of tallgrass prairie in Ben Geren Park progresses, the soil microbiome of restored prairie will more closely mirror those of the virgin prairie.
Complementary Metagenomic Approaches Improve Reconstruction Of Microbial Diversity In A Forest Soil, L. V. Alteio, F. Schulz, R. Seshadri, N. Varghese, W. Rodriguez-Reillo, E. Ryan, D. Goudeau, S. A. Eichorst, R. R. Malmstrom, R. M. Bowers, Laura A. Katz, J. L. Blanchard, T. Woyke
Complementary Metagenomic Approaches Improve Reconstruction Of Microbial Diversity In A Forest Soil, L. V. Alteio, F. Schulz, R. Seshadri, N. Varghese, W. Rodriguez-Reillo, E. Ryan, D. Goudeau, S. A. Eichorst, R. R. Malmstrom, R. M. Bowers, Laura A. Katz, J. L. Blanchard, T. Woyke
Biological Sciences: Faculty Publications
Soil ecosystems harbor diverse microorganisms and yet remain only partially characterized as neither single-cell sequencing nor whole-community sequencing offers a complete picture of these complex communities. Thus, the genetic and metabolic potential of this “uncultivated majority” remains underexplored. To address these challenges, we applied a pooled-cell-sorting-based mini-metagenomics approach and compared the results to bulk metagenomics. Informatic binning of these data produced 200 mini-metagenome assembled genomes (sorted-MAGs) and 29 bulk metagenome assembled genomes (MAGs). The sorted and bulk MAGs increased the known phylogenetic diversity of soil taxa by 7.2% with respect to the Joint Genome Institute IMG/M database and showed clade-specific …
Changes In Soil Microbiology Under Conventional And No-Till Production During Crop Rotation, C. J. Hsiao, G. F. Sassenrath, L. Zeglin, G. Hettiarachchi, C. Rice
Changes In Soil Microbiology Under Conventional And No-Till Production During Crop Rotation, C. J. Hsiao, G. F. Sassenrath, L. Zeglin, G. Hettiarachchi, C. Rice
Kansas Agricultural Experiment Station Research Reports
Soil microbial activity is important for crop production. Soil microbes are involved in nutrient and water cycling within the soil, and interact with crop plants to provide the basic nutrient and water resources needed for crop production. Claypan soils have unique physical characteristics that impact soil biology. This study explored the temporal changes in soil microbiology in a claypan soil under conventional and no-till production during a crop rotation of corn/winter wheat/soybean/fallow commonly planted in southeast Kansas. We found soil microbial activity changed more in the top two inches of soil than in the lower soil layers. Wheat resulted in …
Microbial And Environmental Drivers Of Soil Respiration Differ Along Montane To Urban Transitions, Kerri Ann Russell
Microbial And Environmental Drivers Of Soil Respiration Differ Along Montane To Urban Transitions, Kerri Ann Russell
Theses and Dissertations
In natural ecosystems, like deciduous and coniferous forests, soil CO2 flux or soil respiration is highly variable and influenced by multiple factors including temperature, precipitation, dissolved soil organic carbon (DOC), dissolved organic matter (DOM), and bacterial and fungal biomass and diversity. However, as the human population continues to grow rapidly, so too do urbanized landscapes with unknown consequences to soil respiration. To determine the extent urbanization influences seasonal shifts in microorganisms and environmental drivers alter soil respiration, we evaluated bacterial and fungal communities, soil physiochemical characteristics, and respiration in forested and urbanizing ecosystems in three watersheds across northern Utah, USA. …
Analysis Of The Soil Health Of Pastures Over-Seeded With Winter Annuals, Kathleen M. Bridges
Analysis Of The Soil Health Of Pastures Over-Seeded With Winter Annuals, Kathleen M. Bridges
LSU Doctoral Dissertations
Utilizing winter annuals in livestock pastures reduces feed costs of hay, and provides additional vegetative cover that is beneficial to the soil. However, how this practice affects the soil microbial communities has not been well studied. Two sites were included in this study. Both were commercial cattle grazing operations that over-seeded a mixture of grasses, legumes, and brassicas into pastures dominated by bahiagrass (Paspalum notatum). Soil health was assessed using a suite of physical, chemical, and biological properties. Soil physical assessments included bulk density and aggregate stability. Soil chemical assessments included macronutrients, pH, soil organic matter (SOM), total …
Key Components Of Healthy Soils And Their Role In Crop Production, C. J. Hsiao, Gretchen Sassenrath, Charles Rice, L. Zeglin, G. Hettiarachchi
Key Components Of Healthy Soils And Their Role In Crop Production, C. J. Hsiao, Gretchen Sassenrath, Charles Rice, L. Zeglin, G. Hettiarachchi
Kansas Agricultural Experiment Station Research Reports
Soil health is a confusing term that means different things to different people. To a crop producer, healthy soils are critical for good crop growth and yield. Some soil properties include soil texture, such as the relative percentage of sand, silt and clay; the water content; nutrient levels; organic carbon content; the microbial community; and microbial activity. These properties are determinants of soil health. Our research confirmed that changes in soil management affect the composition and activity of soil microorganisms in surface soils. Greater concentrations of microbial biomass and arbuscular mycorrhizal fungus (AMF) in the no-till agricultural system indicated healthier …
Reduced Snow Cover Alters Root-Microbe Interactions And Decreases Nitrification Rates In A Northern Hardwood Forest, Patrick O. Sorensen, Pamela H. Templer, Lynn M. Christenson, Jorge Durán, Timothy J. Fahey, Melany C. Fisk, Peter M. Groffman, Jennifer L. Morse, Adrien C. Finzi
Reduced Snow Cover Alters Root-Microbe Interactions And Decreases Nitrification Rates In A Northern Hardwood Forest, Patrick O. Sorensen, Pamela H. Templer, Lynn M. Christenson, Jorge Durán, Timothy J. Fahey, Melany C. Fisk, Peter M. Groffman, Jennifer L. Morse, Adrien C. Finzi
Environmental Science and Management Faculty Publications and Presentations
Snow cover is projected to decline during the next century in many ecosystems that currently experience a seasonal snowpack. Because snow insulates soils from frigid winter air temperatures, soils are expected to become colder and experience more winter soil freeze-thaw cycles as snow cover continues to decline. Tree roots are adversely affected by snowpack reduction, but whether loss of snow will affect root-microbe interactions remains largely unknown. The objective of this study was to distinguish and attribute direct (e.g., winter snow-and/ or soil frost-mediated) vs. indirect (e.g., root-mediated) effects of winter climate change on microbial biomass, the potential activity of …
Prokaryotic Diversity In The Rhizosphere Of Organic, Intensive, And Transitional Coffee Farms In Brazil, Adam Caldwell, Livia Silva, Cynthia Da Silva, Cleber Ouverney
Prokaryotic Diversity In The Rhizosphere Of Organic, Intensive, And Transitional Coffee Farms In Brazil, Adam Caldwell, Livia Silva, Cynthia Da Silva, Cleber Ouverney
Faculty Publications, Biological Sciences
Despite a continuous rise in consumption of coffee over the past 60 years and recent studies showing positive benefits linked to human health, intensive coffee farming practices have been associated with environmental damage, risks to human health, and reductions in biodiversity. In contrast, organic farming has become an increasingly popular alternative, with both environmental and health benefits. This study aimed to characterize and determine the differences in the prokaryotic soil microbiology of three Brazilian coffee farms: one practicing intensive farming, one practicing organic farming, and one undergoing a transition from intensive to organic practices. Soil samples were collected from 20 …
Soil Denitrification Fluxes From Three Northeastern North American Forests Across A Range Of Nitrogen Deposition, Jennifer Morse, Jorge Durán, Fred Beall, Eric Enanga, Irena Creed, Ivan Fernandez, Peter Groffman
Soil Denitrification Fluxes From Three Northeastern North American Forests Across A Range Of Nitrogen Deposition, Jennifer Morse, Jorge Durán, Fred Beall, Eric Enanga, Irena Creed, Ivan Fernandez, Peter Groffman
Ivan J. Fernandez
In northern forests, large amounts of missing N that dominate N balances at scales ranging from small watersheds to large regional drainage basins may be related to N-gas production by soil microbes. We measured denitrification rates in forest soils in northeastern North America along a N deposition gradient to determine whether N-gas fluxes were a significant fate for atmospheric N inputs and whether denitrification rates were correlated with N availability, soil O2 status, or forest type. We quantified N2 and N2O fluxes in the laboratory with an intact-core method and monitored soil O2, temperature and moisture in three forests differing …
Microbial Diversity Of A Mediterranean Soil And Its Changes After Biotransformed Dry Olive Residue Amendment, José A. Siles, Caio T.C.C Rachid, Inmaculada Sampedro, Inmaculada García-Romera, James M. Tiedje
Microbial Diversity Of A Mediterranean Soil And Its Changes After Biotransformed Dry Olive Residue Amendment, José A. Siles, Caio T.C.C Rachid, Inmaculada Sampedro, Inmaculada García-Romera, James M. Tiedje
Dartmouth Scholarship
The Mediterranean basin has been identified as a biodiversity hotspot, about whose soil microbial diversity little is known. Intensive land use and aggressive management practices are degrading the soil, with a consequent loss of fertility. The use of organic amendments such as dry olive residue (DOR), a waste produced by a two-phase olive-oil extraction system, has been proposed as an effective way to improve soil properties. However, before its application to soil, DOR needs a pre-treatment, such as by a ligninolytic fungal transformation, e.g. Coriolopsis floccosa. The present study aimed to describe the bacterial and fungal diversity in a …
Cellulose- And Xylan-Degrading Thermophilic Anaerobic Bacteria From Biocompost, M. V. Sizova, J. A. Izquierdo, N. S. Panikov, L. R. Lynd
Cellulose- And Xylan-Degrading Thermophilic Anaerobic Bacteria From Biocompost, M. V. Sizova, J. A. Izquierdo, N. S. Panikov, L. R. Lynd
Dartmouth Scholarship
Nine thermophilic cellulolytic clostridial isolates and four other noncellulolytic bacterial isolates were isolated from self-heated biocompost via preliminary enrichment culture on microcrystalline cellulose. All cellulolytic isolates grew vigorously on cellulose, with the formation of either ethanol and acetate or acetate and formate as principal fermentation products as well as lactate and glycerol as minor products. In addition, two out of nine cellulolytic strains were able to utilize xylan and pretreated wood with roughly the same efficiency as for cellulose. The major products of xylan fermentation were acetate and formate, with minor contributions of lactate and ethanol. Phylogenetic analyses of 16S …
Diversity Of Bacteria And Glycosyl Hydrolase Family 48 Genes In Cellulolytic Consortia Enriched From Thermophilic Biocompost, Javier A. Izquierdo, Maria V. Sizova, Lee R. Lynd
Diversity Of Bacteria And Glycosyl Hydrolase Family 48 Genes In Cellulolytic Consortia Enriched From Thermophilic Biocompost, Javier A. Izquierdo, Maria V. Sizova, Lee R. Lynd
Dartmouth Scholarship
The enrichment from nature of novel microbial communities with high cellulolytic activity is useful in the identification of novel organisms and novel functions that enhance the fundamental understanding of microbial cellulose degradation. In this work we identify predominant organisms in three cellulolytic enrichment cultures with thermophilic compost as an inoculum. Community structure based on 16S rRNA gene clone libraries featured extensive representation of clostridia from cluster III, with one cluster sharing as little as 73% sequence similarity with the closest known relative. In all, 14 new GHF48 gene sequences were added to the known diversity of 35 genes from cultured …
Consequences Of Vegetation Change On The Dynamics Of Labile Organic Matter And Soil Nitrogen Cycling In A Semiarid Ecosystem, Toby D. Hooker
Consequences Of Vegetation Change On The Dynamics Of Labile Organic Matter And Soil Nitrogen Cycling In A Semiarid Ecosystem, Toby D. Hooker
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Sagebrush-dominated ecosystems are being transformed by wildfire, rangeland improvement techniques, and exotic plant invasions. These disturbances have substantial effects on the composition and structure of native vegetation, but the effects on ecosystem C and N dynamics are poorly understood. To examine whether differences in dominant vegetation affect the quantity and quality of plant organic matter inputs to soil, ecosystem C and N pools and rates of plant turnover were compared among historically grazed Wyoming big sagebrush, introduced perennial crested wheatgrass, and invasive annual cheatgrass communities. Since low soil moisture during the summer may inhibit the microbial colonization of plant detrital …
The Soil Acarine Fauna Of Selected Agroecosystems Within The York Shire, Derek Juan Swarts
The Soil Acarine Fauna Of Selected Agroecosystems Within The York Shire, Derek Juan Swarts
Theses : Honours
The practice of broadacre, dryland farming is known to affect the physico-chemical structure of the soil but our knowledge or its impact on the biological structure is rudimentary. The soil acarine (mite) communities which inhabit these soils display a variety of responses to this agricultural practice. With the increasing focus on developing ecologically sustainable agricultural techniques, an understanding of the soil acari becomes increasingly important. This study investigated the impact of two agricultural practices (designated here us Conventional and Non-Conventional) upon the abundance and diversity of the soil acari. Conventional agricultural practice involved broad scale chemical application to weeds and …
The Effects Of 2,4-Dichlorophenoxyacetic Acid On The Diazotrophic Soil Microbial Community, Denise M. Brown
The Effects Of 2,4-Dichlorophenoxyacetic Acid On The Diazotrophic Soil Microbial Community, Denise M. Brown
Master's Theses
This study measures the effects of 2,4- dichlorophenoxyacetic acid (2,4-D) on growth and nitrogen fixation capabilities of the native microbial soil community during eight field trials conducted over an eight month period. After application of 2,4-D, soil samples were analyzed for enumeration of diazotrophic and general microbial communities, and for nitrogenase activity as measured by the acetylene reduction assay (ARA). Statistical analyses were conducted using Minitab. Results show that 2,4-D significantly stimulated growth of diazotrophic microbes in 87.5% of the trials and was found to have a stimulatory growth effect on the general microbes in 62.5% of the trials. A …
So You Want To Be A Soil Microbiologist?, Mark S. Coyne
So You Want To Be A Soil Microbiologist?, Mark S. Coyne
Plant and Soil Sciences Faculty Book Gallery
A tongue-in-cheek look at soil microbiology.