Life Sciences Commons^™

Quantifying The Respiratory Plasticity Of Common Fishes Of The Indian River Lagoon, Logan Exton

Honors Theses

Increasing water temperatures resulting from global climate change introduce new energetic demands for marine organisms. Higher energy input will be required to cope with a subsequently higher metabolic rate, affecting all aspects of an individual’s life and therefore their survival. Because estuaries act as a link between rivers and oceans, they and their inhabitants are considered to be the most threatened by climate change. Therefore, it is crucial to understand how these organisms will respond to increased stressors due to climate change. Checkered pufferfish (Sphoeroides testudineus) are among the most common teleost fish in the Indian River Lagoon, …

Thermoregulation In Nocturnal Volant Endotherms Occupying Hot Deserts, William A. Talbot

Biology ETDs

ABSTRACT

The study of thermoregulation is of growing concern in this era of rapid climate change. Earlier studies such as those pioneered by Scholander and Bartholomew directed focus toward the study of endotherm survival in conditions of cold weather adversity. As techniques evolved in the measurement of parameters quantifying thermoregulation, metabolism and energy allocation, the standardization procedures has increased the comparability of data from diverse taxa.

We can now search historical records and current research for explanations to the changes in the distribution, migration, extirpation or survival of animal and plant populations through time. It is increasingly common to observe …

Relating Metabolic Phenotypes To Movement Behavior In Brook Trout, Jacob E. Bowman

All NMU Master's Theses

Brook trout movement-related life history strategies vary considerably and range from individuals that stay within the same 100 meters their entire life to individuals that are potamodromous or anadromous. Potential drivers of movement life histories have been the subject of much research in fish, with genetic subpopulation explanations often failing to explain the phenomenon. Metabolic phenotypes have been suggested as a possible driver for expression of different movement life histories. I investigated if metabolic phenotypes are related to movement strategies within a population of brook trout (Salvelinus fontinalis) in Unnamed Creek, a tributary to the Rock River, Alger …

Metabolic Rate, Critical Oxygen Partial Pressure, And Oxygen Supply Capacity Of Farfantepenaeus Duorarum At Their Lower Thermal Limit, Alexandra L. Burns

USF Tampa Graduate Theses and Dissertations

Temperature and environmental oxygen availability affect oxygen supply and demand in ectotherms, which are hypothesized to control the geographic limits of many marine species. The oxygen supply capacity (α) is calculated from commonly measured metabolic traits, including the standard metabolic rate (SMR) and critical oxygen partial pressure at SMR (Pcrit). It may be used to estimate the metabolic capacity and aerobic scope across changes in temperature and oxygen partial pressures as α reflects adaptations of the cardiorespiratory system to meet maximum energy demands at a given oxygen partial pressure (PO2). In this study, α was measured for the Tampa Bay …

Effect Of Hyposaline Stress On Metabolic Rate Of The Invasive Mussel Mytilus Galloprovincialis, Hayley C. Hart

Honors Thesis

Climate change is predicted to decrease ocean salinity as the polar ice caps melt and the occurrence of precipitation events increases. Decreased ocean salinity (hyposalinity) may negatively impact marine invertebrates, especially marine mussels, as they are osmoconformers and their internal osmolarity depends on the solute concentration of their environment. Hyposalinity stress may influence cell function and alter mussel physiology. To compensate for the change in the environment, mussels may expend more energy to restore internal osmolarity, which can be assessed by quantifying metabolic rate. Thus, the purpose of our research was to examine the physiological response of Mytilus galloprovincialis, …

Living On The Edge: Thermophysiology Of The Southern Flying Squirrel At Its Northern Range Margin, Vanessa R. Hensley

Electronic Theses and Dissertations

Climate change has the potential to upset entire ecological systems, making predictive models of the utmost importance. The incorporation of physiological parameters into predictive models not only bolsters their accuracy but also provides a mechanistic explanation for ecological changes already observed and those yet to come. North American flying squirrels, for example, have already experienced dramatic range shifts northward over recent decades, with climate change being the suspected driver. While other studies have focused on warming winter temperatures, I explored the hypothesis that rising summer temperatures were driving the observed range shifts. Unable to find a reliable population of the …

Cold Tolerance, Temperature Mediated Discontinuous Gas Exchange, And Emergence Of The Blue Orchard Mason Bee (Osmia Lignaria), Logan Kral

All Master's Theses

The relationship between low temperatures, emergence and supercooling point of Osmia lignaria were the subject of this study. One hundred sixty-eight bees were subjected to 5 pre-wintering temperature treatments (two constant temperature controls - 22^oC, 14^oC, one of which with and one without a 12h photoperiod, and three 12h:12h thermoperiod treatments – 14:10^oC, 14:5^oC, and 14:0^oC) and were then evaluated in terms of emergence time and post-emergence vigor. An additional 70 bees were tested for metabolic rate and discontinuous gas exchange in response to test temperature conditions. An additional sample …

Physiological Response Of The Giant Acorn Barnacle, Balanus Nubilus, To Air Exposure, Emily Jane Resner

Master's Theses

The giant acorn barnacle, Balanus nubilus, is a resident of the subtidal and low intertidal rocky shoreline on the Pacific Coast of North America (Alaska to Baja California). B. nubilusis notable for having the largest muscle fibers in the animal kingdom; fiber diameters that can exceed 3mm in adults! At such extreme sizes these muscle cells may be at risk for insufficient oxygen delivery to mitochondria owing to low SA:V ratios and long intracellular diffusion distances. Oxygen limitation to these muscles may be further exacerbated during low tide air exposure (emersion) or environmental hypoxia events, which are increasing …

Escaping The Arrhenius Tyranny: Metabolic Compensation During Exposure To High Temperature In Daphnia, Bret L. Coggins

Electronic Theses and Dissertations

Poikilothermic organisms experience trade-offs by differential physiological demands generated by temperature extremes. Many such organisms exhibit acclimatory effects, adjusting their metabolism and physiology to recently experienced temperatures. One such acclimatory effect is metabolic compensation, the deceleration of biological rates below Arrhenius expectations. Daphnia magna is eurythermal, and if acclimated to mildly stressful temperatures first, survives longer in lethal temperatures. This study examined the effect of ambient temperature (5°C-37°C) and acclimation history (lifetime at 10°C or 25°C) on the oxygen consumption rates of 8 genotypes of Daphnia with high or low acute temperature tolerance. There were decelerations of respiratory rates across …

Comparison Of Thermal Tolerance And Standard Metabolic Rate Of Two Great Lakes Invasive Fish Species, Ken G. Drouillard, David A. Feary Dr, Xin Sun, Jessica A. O'Neil, Todd Leadley, Tim B. Johnson Dr

Great Lakes Institute for Environmental Research Publications

Round goby (Neogobius melanostomus) and western tubenose goby (Proterorhinus semilunaris) invaded the Laurentian Great Lakes at approximately the same time and area yet have shown substantial differences in their post-invasion success with more rapid establishment and development of much larger abundances of round goby populations throughout the invaded habitat. In this study, we compared differences in physiological performance (thermal tolerance and standard metabolic rate) between round and tubenose goby collected from the Huron-Erie corridor. Tubenose goby were observed to have lower thermal tolerance but exhibited similar standard metabolic rate across environmental temperatures compared to round goby. …

Effects Of Winter And Summer Thermal Variability Regimes On Growth And Metabolism In Brook Trout (Salvelinus Fontinalis), Spenser Chicoine

All NMU Master's Theses

Brook trout (Salvelinus fontinalis) is a cold-water species with a narrow range of thermal tolerance. Climate change projections show that in addition to increases in average temperatures worldwide, daily thermal variability is increasing. Such conditions could prove challenging for brook trout and other ectotherms that are adapted to small temperature ranges. This research investigates how the growth and metabolic capacities of brook trout respond to long-term exposure to thermal variation. Groups of fish were reared under three thermal regimes: constant baily temperature, a 4°C daily range, and an 8°C daily range in a controlled lab setting. This was …

Physiological Ecology Of Four Endemic Alabama Species And The Exotic Asiatic Weatherfish, Misgurnus Anguillicaudatus (Cantor, 1842), Lindsay M. White, Mark E. Meade, Benjamin A. Staton

Southeastern Fishes Council Proceedings

The occurrence of Asiatic Weatherfish, Misgurnus anguillicaudatus, in Alabama, a state known for its rich biodiversity, has generated concern among conservation managers. The current study used respirometry techniques to investigate the effects of increasing temperature on four native southeastern fishes (one cyprinid, two percids, and one elassomid) and the non-native M. anguillicaudatus. A minimum of five individuals of each species were used, and three experimental temperatures were chosen to represent spring and summer averages of northeast Alabama streams (15, 20, and 25°C). Overall, mean standard metabolic rates (SMRs) for M. anguillicaudatus were low (97.01, 127.75, and 158.50 mg …

Accuracy And Precision Of Quantitative 31p-Mrs Measurements Of Human Skeletal Muscle Mitochondrial Function, Gwenael Layec, J. R. Gifford, Joel D. Trinity, Corey R. Hart, Ryan S. Garten, Song-Young Park, Yann Le Fur, Eun-Kee Jeong, Russell S. Richardson

Health and Kinesiology Faculty Publications

Although theoretically sound, the accuracy and precision of 31P-magnetic resonance spectroscopy (31P-MRS) approaches to quantitatively estimate mitochondrial capacity are not well documented. Therefore, employing four differing models of respiratory control [linear, kinetic, and multipoint adenosine diphosphate (ADP) and phosphorylation potential], this study sought to determine the accuracy and precision of 31P-MRS assessments of peak mitochondrial adenosine-triphosphate (ATP) synthesis rate utilizing directly measured peak respiration (State 3) in permeabilized skeletal muscle fibers. In 23 subjects of different fitness levels, 31P-MRS during a 24-s maximal isometric knee extension and high-resolution respirometry in muscle fibers from the vastus lateralis was performed. Although significantly …

Top Predators Negate The Effect Of Mesopredators On Prey Physiology, Maria Palacios, Shaun S. Killen, Lauren E. Nadler, James R. White, Mark I. Mccormick

Marine & Environmental Sciences Faculty Articles

1. Predation theory and empirical evidence suggest that top predators benefit the survival of resource prey through the suppression of mesopredators. However, whether such behavioural suppression can also affect the physiology of resource prey has yet to be examined.
2. Using a three‐tier reef fish food web and intermittent‐flow respirometry, our study examined changes in the metabolic rate of resource prey exposed to combinations of mesopredator and top predator cues.
3. Under experimental conditions, the mesopredator (dottyback, Pseudochromis fuscus ) continuously foraged and attacked resource prey (juveniles of the damselfish Pomacentrus amboinensis ) triggering an increase in prey O2 uptake by 38 ± …

Effects Of Salinity And Ph Change On The Physiology Of An Estuarine Fish Species, Fundulus Heteroclitus Heteroclitus, Shauna M. Tietze

Electronic Theses and Dissertations

Sapelo Island, Georgia is a pristine environment characterized by many variable salt marsh habitats. Throughout the island, salinity ranges from freshwater to full strength seawater and acidity levels range from low pH (pH < 4.4) to above neutral (pH > 8). Fundulus heteroclitus (Mummichog) is a resident organism of the salt marsh environment that has evolved coping strategies to withstand fluctuations in temperature, salinity and pH. Field and laboratory studies have shown F. heteroclitus to exhibit broad tolerance of individual environmental stressors; however, there is limited information on their ability to handle multiple stressors simultaneously. The goal of this study was to determine if exposure to low …

Streamwise Vortices Destabilize Swimming Bluegill Sunfish (Lepomis Macrochirus), Anabela Maia, Alex P. Sheltzer, Eric D. Tytell

Faculty Research & Creative Activity

In their natural environment, fish must swim stably through unsteady flows and vortices, including vertical vortices, typically shed by posts in a flow, horizontal cross-flow vortices, often produced by a step or a waterfall in a stream, and streamwise vortices, where the axis of rotation is aligned with the direction of the flow. Streamwise vortices are commonly shed by bluff bodies in streams and by ships' propellers and axial turbines, but we know little about their effects on fish. Here, we describe how bluegill sunfish use more energy and are destabilized more often in flow with strong streamwise vorticity. The …

Empirical Modelling Of Solid-Blocking Effect In A Blazka Respirometer For Gag, A Large Demersal Reef Fish, Richard J. Kline, Daryl C. Parkyn, Debra J. Murie

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

The effect of solid-body blocking in a large (257 l) Blazka-style respirometer was examined in gag Mycteroperca microlepis. Gag ranging from 376-690 mm total length were tested at velocities from 10 to 91 cm s-1 . The solid blocking effect (SBE) due to fish size, swimming speed, and water temperature was investigated by direct measurement of flow velocity at the area of the fish’s maximum girth as compared to measurements at the rear (center) of the swimming chamber. Three models were compared as corrections for the SBE: the standard Bell and Terhune (BT), a novel modification of the Bell and …

Supplemental Materials For Largemouth Bass Respirometry Research: Data Archived In Support Of Open-Access Publication, Jan-Michael Hessenauer, Jason Vokoun

Department of Natural Resources and the Environment Articles

Fisheries induced evolution (FIE) is hypothesized to cause changes in the life history, physiology and behavior of exploited fish stocks. Most studies evaluating FIE have occurred in exploited marine stocks which offer little opportunity for replication and few unexploited stocks exist for comparison. Inland recreational fisheries, however, offer the opportunity to replicate experiments in multiple systems, and while rare, unexploited populations exist for comparison. We measured the resting metabolic rate of four populations of largemouth bass, two exploited and two unexploited, to evaluate whether patterns of resting metabolic rates conformed to expectations for fisheries induced evolution. This document contains the …