Life Sciences Commons^™

Genome-Wide Association Studies Revealed Dartseq Loci Associated With Seed Traits In Turkish Common Bean Germplasm, Muhammad Azhar Nadeem, Faheem Shahzad Baloch

Turkish Journal of Agriculture and Forestry

Seed traits are very important agronomic traits that contribute significantly into crop yield and are considered useful indicators to monitor the inheritance of quantitative traits influenced by genotype and environmental conditions. To investıgate genomic regions useful for breeding high-yielding and highly nutritious common bean (Phaseolus vulgaris L.) cultivars, a mini core collection of Turkish common bean germplasm was screened under five environments and two locations for seed traits (seed width, seed yield/plant (SYP), and hundred seed weight). The results of analysis of variance (ANOVA) across the environments revealed significant effects of genotypes and genotype by environment interaction (G × E) …

The Biosynthesis Of Non-Protein Sulfur Amino Acid In Developing Seeds Of Common Bean, Zixuan Lu

Electronic Thesis and Dissertation Repository

The protein quality of common bean (Phaseolus vulgaris) is associated with the level of dietary essential sulphur amino acids – methionine and cysteine. Extra sulphur that cannot be stored in the protein pool accumulates as the non-protein amino acid S-methylcysteine (S-methylCys) and its dipeptide γ-glutamyl-S-methylcysteine (γ-Glu-S-methylCys). Previous studies have indicated that S-methylhomoglutathione (S-methylhGSH) is present in the developing seed of common bean. It is hypothesized that S-methylhGSH is the key intermediate in the biosynthetic pathway of the γ-Glu-S-methylCys that leads to …

Unlocking The Genomic Regions Associated With Seed Protein Contents In Turkishcommon Bean Germplasm Through Genome-Wide Association Study, Faheem Shahzad Baloch, Muhammad Azhar Nadeem

Turkish Journal of Agriculture and Forestry

Human body needs sufficient quantity of protein on daily basis for the normal functioning. Dietary protein in sufficient quantity is becoming a key issue for huge population of the world particularly in developing countries. Present investigation aimed to explore the phenotypic variation and to investigate the genomic regions associated with seed protein contents in Turkish common bean germplasm collected from 19 provinces. Field experiments were conducted according to augmented block design at two locations (Bolu and Sivas) for two consecutive years (2017 and 2018). Analysis of variance revealed highly significant environmental effects (p < 0.05) on seed protein contents within environments, while genotype by environment interaction revealed nonsignificant effects. Overall mean protein contents were 36.36%, while Balikesir-3 and Hakkari-38 yielded minimum (31.64%) and maximum (40.70%) protein contents. Among 19 provinces, accessions from Hakkari and Erzincan provinces disclosed maximum and minimum mean protein contents respectively. Scatter plot between seed protein contents and plant height divided the accessions according to their protein contents and plant height. A total of eight stable accessions were selected and can be recommended for future breeding activities. The implemented constellation plot separated the studied germplasm into two populations on the basis of their protein contents. Genotyping by sequencing resulted 7900 DArTseq markers was used for marker-trait association and a total of 11 markers showed significant association for protein contents. During this study, DArT-3365703 marker was identified in both locations (Sivas and Bolu) during 2017 and might be used for future common bean breeding. Physical map revealed the presence narrow regions between identified markers on Pv02 and Pv05. Therefore, these regions should be considered for future breeding activities. We are confident that results of present study will be helpful for marker-assisted breeding of common bean.

Genetic Diversity Of Pinto And Fresh Bean (Phaseolus Vulgaris L.) Germplasm Collected From Erzincan Province Of Turkey By Inter-Primer Binding Site (Ipbs) Retrotransposon Markers, Hali̇l İbrahi̇m Öztürk, Ati̇lla Dursun, Arash Hosseinpour, Kami̇l Hali̇loğlu

Turkish Journal of Agriculture and Forestry

In this study, genetic diversity among 71 bean genotypes (41 pinto beans and 30 fresh bean genotypes) and 4 commercial cultivars (Aleyna, Gina, Perolar, and Serra) commonly grown at Erzincan province in Turkey were investigated. The iPBS (inter-primer binding site) markers were used to investigate the genetic diversity of the genotypes. Polymorphism ratio was identified as 100% in all primers. Twenty-seven primers were studied and number of alleles in primers varied between 13?69 (with an average value of 37.14). According to cluster analysis based on iPBS data, genotypes were divided into 2 groups. Polymorphic information content (PIC) values varied between …

El Cultivo De Fríjol (Phaseolus Vulgaris) Como Alternativa De Producción Para La Comunidad En El Municipio De San Pablo De Borbur, Boyacá, Efrén Duván Gutiérrez Hernández

Ingeniería Agronómica

En el municipio de San Pablo de Borbur, el fríjol (Phaseolus vulgaris) es un cultivo poco implementado por los agricultores, generando una baja oferta del producto en el mercado local y un escaso conocimiento de su manejo, llevando a la aparición de problemas de plagas y enfermedades en este. Debido a este panorama, se implementó un cultivo de fríjol (P. vulgaris) variedad Calima, con el objetivo de mostrar a la comunidad las actividades técnicas para un excelente manejo del cultivo y como componente agronómico del proyecto productivo en zona de origen. El proyecto productivo se ejecutó de manera exitosa, debido …

Molecular Cytogenetic Characterization Of Common Bean (Phaseolus Vulgaris L.) Accessions, Gülsemi̇n Savaş Tuna, Gülru Yücel, Tansel Kaygisiz Aşçioğul, Duygu Ateş, Dursun Eşi̇yok, Muhammed Bahatti̇n Tanyolaç, Meti̇n Tuna

Turkish Journal of Agriculture and Forestry

With an annual global production of approximately 25 million tons, the common bean (Phaseolus vulgaris L), a member of the genus Phaseolus, is one of the major protein sources used as food for humans. In this study, it was aimed to investigate the genome size of the common bean genetic resource collection (154 common bean accessions) in Turkey by flow cytometry (FCM) and determine whether geographical variables affected the genome size. In addition, the number and distribution of 5S and 45S ribosomal DNA loci were designated by performing a fluorescence in situ hybridization (FISH) analysis in some of the accessions. …

El Frijol (Phaseolus Vulgaris) Variedad Diacol Calima Como Opción De Asocio En El Cultivo De Naranja En El Municipio De Lejanias Meta, Luis Antonio Serrato Delgado

Ingeniería Agronómica

El corregimiento de Cacayal, está ubicado en el municipio de Lejanias Meta considerado como uno de los municipios pioneros en la producción agrícola y pecuaria, considerada la capital frutícola de la abundancia. La ejecución del proyecto productivo en la zona de origen consistió en establecer un sistema de producción 5.000 m2 frijol Diacol Calima (Phaseolus .vulgaris), en asocio con un cultivo de naranja variedad tangelo, como alternativa de aprovechamiento del terreno; esta alternativa estuvo ligada a la ejecución de componentes de seguimiento impartidos por parte del programa de ingeniería agronómica de la Universidad de La Salle del campus universitario Utopía. …

Single And Multi-Trait Gwas Identify Genetic Factors Associated With Production Traits In Common Bean Under Abiotic Stress Environments, Atena Oladzad, Timothy G. Perch, Juan Carlos Rosas, Samira Mafi Moghaddam, James Beaver, Steve E. Beebe, Jimmy Burridge, Celestina Nhagupana Jochua, Magalhaes Amade Miguel, Phillip N. Miklas, Bodo Raatz, Jeffery W. White, Jonathan Lynch, Phillip E. Mcclean

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The genetic improvement of economically important production traits of dry bean (Phaseolus vulgaris L.), for geographic regions where production is threatened by drought and high temperature stress, is challenging because of the complex genetic nature of these traits. Large scale SNP data sets for the two major gene pools of bean, Andean and Middle American, were developed by mapping multiple pools of genotype-by-sequencing reads and identifying over 200k SNPs for each gene pool against the most recent assembly of the P. vulgaris genome sequence. Moderately sized Bean Abiotic Stress Evaluation (BASE) panels, consisting of genotypes appropriate for production in …

Establecimiento De Un Sistema De Producción De 2.500 M2 De Habichuela (Phaseolus Vulgaris) Para El Mejoramiento Socioeconómica Del Municipio De Algeciras-Huila, Juan Felipe Ovalle Muñoz

Ingeniería Agronómica

Debido al aumento del área sembrada de café (Coffea arabica) y a la reducción de cultivos de frutales y transitorios en el municipio de Algeciras, se realizó el siguiente proyecto productivo, el cual tuvo cuatro componentes. En el componente agronómico, se realizó la implementación de un cultivo de habichuela (Phaseolus vulgaris) variedad Blue Lake como método demostrativo de la producción tecnificada, reduciendo los costos de producción mediante un manejo integrado de plagas, enfermedades y arvenses, buscando la disminución de las aplicaciones de agroquímicos. Entretanto el componente investigativo constó de un ensayo en el cual se hizo uso de desechos orgánicos …

Gge Biplot Analysis Of Yield Stabi Lity For Andean Dry Bean Accessions Grown Under Different Abiotic Stress Regimes In Tanzania, E. J. Mndolwa, S. N. Msolla, T. G. Porch, Phillip N. Miklas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Large seeded Andean dry beans (Phaseolus vulgaris L.) are most preferred in Africa. However, low soil fertility and increasing drought stress conditions due to climate change are among the challenges faced by farmers in this region. The purpose of this study was to identify Andean genotypes with yield stability and to identify the best environments for selection of this trait. GGE-Biplot analysis was used to examine 183 Andean bean genotypes in Tanzania. Results indicated significant environment (E), genotype (G) and genotype x environment (GE) effects for seed yield. The environment explained 46%, genotype 20%, and GE 34% of the total …

Generation And Validation Of Genetic Markers For The Selection Of Carioca Dry Bean Genotypes With The Slow-Darkening Seed Coat Trait, Renata C. Alvares, Robert Stonehouse, Thiago L.P.O. Souza, Patrícia G.S. Melo, Phillip N. Miklas, Kirstin E. Bett, Leonardo C. Melo, Luana A. Rodrigues, Lorena L. Souza, Helton S. Pereira

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Slow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepe´rola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Nota´vel and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd- 1158 and PVM02TC116 and with a …

The Role Of Genotype And Production Environment In Determining The Cooking Time Of Dry Beans (Phaseolus Vulgaris L.), Karen A. Cichy, Jason A. Wiesinger, Matthew Berry, Susan Nchimbi‐Msolla, Deidre Fourie, Timothy G. Perch, Daniel Ambechew, Phillip N. Miklas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Dry bean (Phaseolus vulgaris L.) is a nutrient‐dense food rich in proteins and minerals. Although a dietary staple in numerous regions, including Eastern and Southern Africa, greater utilization is limited by its long cooking time as compared with other staple foods. A fivefold genetic variability for cooking time has been identified for P. vulgaris, and to effectively incorporate the cooking time trait into bean breeding programs, knowledge of how genotypes behave across diverse environments is essential. Fourteen bean genotypes selected from market classes important to global consumers (yellow, cranberry, light red kidney, red mottled, and brown) were grown …

Studies On The Physicochemical Characterization Of Flours And Protein Hydrolysates From Common Beans, Hollman Andres Motta Romero

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Pulses represent a rich source of nutrition and have several potential health benefits such as the reduction of the risk of developing cardiovascular diseases, obesity, and diabetes. Beans from the species Phaseolus vulgaris and Cicer arietinum are important crops raised in some regions of the United States, including Western Nebraska. Nevertheless, the traditional preparation procedures involved in the cooking of these beans are tedious and time-consuming, thus leading to the underutilization of these resources. Therefore, the present work focused on evaluating alternative utilizations of these beans in order to improve their economic value.

One alternative to improve the consumption of …

Midwest Vegetable Trial Report For 2017, Elizabeth Maynard, Brad R. Bergefurd, Wenjing Guan, Petrus Langenhoven

Purdue Fruit and Vegetable Research Reports

This is a compilation of 19 research trial reports from six land-grant universities in the Midwestern United States. Crops include green beans, cantaloupe, cucumber, ornamental corn, pepper, potato, pumpkin, butternut squash, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed specific growing practices: plant spacing for sweet corn.

Midwest Vegetable Trial Report For 2017, Elizabeth Maynard, Brad R. Bergefurd, Wenjing Guan, Petrus Langenhoven

Midwest Vegetable Trial Reports

This is a compilation of 19 research trial reports from six land-grant universities in the Midwestern United States. Crops include green beans, cantaloupe, cucumber, ornamental corn, pepper, potato, pumpkin, butternut squash, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed specific growing practices: plant spacing for sweet corn.

The Genetic Improvement Of Protein Quality In Common Bean (Phaseolus Vulgaris L.), Rosa Cecilia Viscarra Torrico

Electronic Thesis and Dissertation Repository

Abstract

The common bean (Phaseolus vulgaris L.) has a high seed protein content, between 20 and 30%. The protein quality in common is considered low because of the suboptimal levels of methionine and cysteine in the seed. Phaseolin, the main seed storage protein, accounts for 30-50% of the total seed protein content. Phaseolin only contains about 0.5 to 0.80% methionine. The suggested nutritional requirements for methionine-cysteine in the human diet are between 2.5 and 2.6 %. Previous studies on the germplasm SMARC1N-PN1 showed that deficiency in phaseolin and lectins leads to increased methionine-cysteine up to 2.6% in the bean …

Population Structure And Phenotypic Variation Of Sclerotinia Sclerotiorum From Dry Bean (Phaseolus Vulgaris) In The United States, Zhian N. Kamvar, Bimal Sajeewa Amaradasa, Rachana Jhala, Serena Mccoy, James R. Steadman, Sydney Everhart

Department of Plant Pathology: Faculty Publications

The ascomycete pathogen Sclerotinia sclerotiorum is a necrotrophic pathogen on over 400 known host plants, and is the causal agent of white mold on dry bean. Currently, there are no known cultivars of dry bean with complete resistance to white mold. For more than 20 years, bean breeders have been using white mold screening nurseries (wmn) with natural populations of S. sclerotiorum to screen new cultivars for resistance. It is thus important to know if the genetic diversity in populations of S. sclerotiorum within these nurseries (a) reflect the genetic diversity of the populations in the surrounding region and (b) …

Control Of White Mold Of Dry Bean And Residual Activity Of Fungicides Applied By Chemigation, Thomas J.J. Miorini, Carlos G. Raetano, Sydney E. Everhart

Department of Plant Pathology: Faculty Publications

Sclerotinia sclerotiorum is a necrotrophic fungal pathogen that causes white mold of dry bean (Phaseolus vulgaris L.). Chemigation with fungicides is used for disease control, but effectiveness of this application method and impact of irrigation level on residual fungicide activity in the plant over time under field conditions has not been well characterized. To assess the best method of application and fungicide for disease control, we conducted field studies in three field sites in São Paulo State in Brazil. Contact fungicide, fluazinam, was applied via center pivot at three irrigation levels (2.5, 5.1, 10.1 mm) at the Itaí field …

Regeneration And Transformation Of Common Bean (Phaseolus Vulgaris L.), Iqbal Singh

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Common bean is the most important grain legume for direct human consumption and serves as main source of dietary protein for millions of people in developing world. Genetic transformation methods can serve as an important tool to complement traditional plant breeding methods for common bean improvement. Low transformation frequencies and unstable genetic integration associated with biolistic methods limits its use for routine transformation of common bean. In an attempt to develop Agrobacterium tumefaciens mediated transformation protocol for common bean, potential of primary leaves as an explant was analyzed following unsuccessful results with other tissues. Primary leaf explants were prepared from …

Growth, Sucrose Synthase, And Invertase Activities Of Developing Phaseolus Vulgaris L. Fruits, S. Sung, Wen-Jang Shieh, Donald Geiger, Clanton Black

Donald R. Geiger

Activities of the sucrose-cleaving enzymes, acid and neutral invertase and sucrose synthase, were measured in pods and seeds of developing snap bean (Phaseolus vulgaris L.) fruits, and compared with 14C-import, elongation and dry weight accumulation. During the first 10 d post-anthesis, pods elongated rapidly with pod dry weight increase lagging behind by several days. The temporal patterns of acid invertase activity and import coincided closely during the first part of pod development, consonant with a central role for this enzyme in converting imported sucrose during pod elongation and early dry weight accumulation. Later, sucrose synthase became the predominant enzyme of …

Generation Of Transgenic Sugar Beet (Beta Vulgarism L.) Overexpressing The Polygalacturonase Inhibiting Protein 1 Of Phaseolus Vulgaris (Pvpgip1) Through Agrobacterium-Mediated Transformation, Reza Mohammadzadeh, Mostafa Motallebi, Mohamadreza Zamani, Zahra Moghaddassi Jahromi, Peyman Norouzi, Manuel Benedetti, Giulia De Lorenzo

Turkish Journal of Agriculture and Forestry

Numerous diseases caused by fungal pathogens influence the annual production of sugar beet. In order to obtain a plant resistant to fungi, genetic transformation has been applied to the sugar beet. To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes (CWDEs); polygalacturonases (PGs) are pathogenicity factors produced at the earlier stages of a fungal infection that depolymerize the homogalacturonan. One of the strategies used by plants to limit the degradation of the cell wall polysaccharides by fungal CWDEs is the production of proteinaceous inhibitors. Against fungal, microbial, and insect PGs, plants produce cell wall-associated polygalacturonase-inhibiting proteins (PGIPs). …

Nanoceria Exposure To Kidney Beans (Phaseolus Vulgaris): Implications On Plant Physiology, Nutrition And Their Transfer To Next Trophic Level, Sanghamitra Majumdar

Open Access Theses & Dissertations

Previous studies investigating the effects of cerium oxide nanoparticles (nanoceria, nCeO2) on plants have primarily focused on the physiological and biochemical changes at early growth stages. Comprehensive information on the effects of nCeO2 through the entire life cycle of plants and the nutritional quality of the edible tissues is limited. No studies have been reported on the interactions between nCeO2 and common beans (Phaseolus vulgaris). Common beans are leguminous crops, which are societally important due to their nutritional benefits. The beans are rich in proteins and essential nutrients like folate, iron, zinc, molybdenum and magnesium, and are consumed worldwide. This …

Screening Of Common Bean (Phaseolus Vulgaris L.) Cultivars Against Root-Lesion Nematode Species, Mehmet Ali̇ Söğüt, Fatma Gül Göze, Tufan Önal, Zübeyi̇r Devran, Muhammet Tonguç

Turkish Journal of Agriculture and Forestry

In this study, screening of 15 Phaseolus vulgaris cultivars and genotypes (Zülbiye, Kınalı, Perla, Kwintus, Özayşe, Tokat Sırık, Musica, Şelale, Nadide, Gina, Serra, Karabağ, Funda, and Lepus) and 1 Pisum sativum cultivar (Araka) for host suitability to 4 root-lesion nematodes, Pratylenchus thornei, P. crenatus, P. neglectus, and P. penetrans, was carried out in 2010 and 2011 under controlled conditions. The experiment was set up in a completely randomized block design with 7 replicates. Approximately 1000 adults + juveniles + eggs of root-lesion nematodes in 10 mL of distilled water were inoculated around the base of each bean seedling. The experiments …

Phylogenetic Analysis Of Rhizoctonia Solani Ag-4 Isolates From Common Beans In Black Sea Coastal Region, Turkey, Based On Its-5.8s Rdna, Meli̇ke Çebi̇ Kiliçoğlu, İbrahi̇m Özkoç

Turkish Journal of Biology

In this study, 114 Rhizoctonia solani isolates were obtained from diseased common bean (Phaseolus vulgaris L.) plants from the Black Sea coastal region of Turkey. Genetic variation was determined among R. solani anastomosis group 4 (AG-4) subgroups (AG-4 HG-I and AG-4 HG-II). ITS-5.8 rDNA sequences of these isolates were aligned with other known R. solani sequences from GenBank, and distance and parsimony analysis were used to determine phylogenetic relationships. The R. solani AG-4 isolates were placed in 2 main lineages, corresponding to AG-4 HG-I and AG-4 HG-II. Based on phylogenetic analysis, genetic variations were shown in the AG-4 subgroups (especially …

Assessing Genetic Diversity In The Web Blight Pathogen Thanatephorus Cucumeris (Anamorph = Rhizoctonia Solani) Subgroups Ag-1-Ie And Ag-1-If With Molecular Markers, N. González, James R. Steadman, Robert Higgins, Kent M. Eskridge

Department of Plant Pathology: Faculty Publications

Web blight, an important foliar disease of dry beans in the Americas, is a challenge to manage. We studied genetic variation of 92 isolates of Rhizoctonia solani subgroups AG-1-IE and AG-1-IF using DNA fingerprinting methods and mycelial compatibility grouping. The isolates were collected over 13 years from bean fields in the Dominican Republic, Honduras and Puerto Rico. Cluster and AMOVA analysis of combined data from two universal rice primers and two internal sequence repeats revealed significant genetic variation among and within populations of both subgroups. Variation was influenced by geographic origin and sampling year for AG-1-IE isolates and geographic origin …

Phaseolus Beans: Impact On Glycaemic Response And Chronic Disease Risk In Human Subjects, Andrea M. Hutchins, Donna M. Winham, Sharon V. Thompson

Donna Winham

Consumption of Phaseolus vulgaris bean species such as pinto, black, navy or kidney may be beneficial in the prevention and treatment of chronic diseases. In particular, conditions that are promoted by increased glycaemic stress (hyperglycaemia and hyperinsulinaemia) including diabetes, CVD and cancer seem to be reduced in individuals who eat more of these beans. The present paper discusses the influence of P. vulgaris species on glycaemic response and the impact that relationship may have on the risk of developing diabetes, CVD and cancer.

The Effects Of Agitation Upon Plant Anatomy In Phaseolus Vulgaris, Craig Sutter, Nicholas Huron

Featured Research

The earthquake that struck Japan’s coast a month ago gained national attention for the damage caused by the tsunami that was caused by the earthquake. Japan’s coast is subject to multiple earthquakes each year with tremors and aftershocks following. The quakes are bound to have an effect on the growth and development of Camellia sinensis, the tea plant, which is one of the most important exports of Japan. Due to the time restraints of this project, C. sinensis were not tested, instead two week old Phaseolus vulgaris were used to show the effects of constant agitation which might mimic …

Impact Of Soil Drought Stress On Photochemical Efficiency Of Photosystem Ii And Antioxidant Enzyme Activities Of Phaseolus Vulgaris Cultivars, Rabi̇ye Terzi̇, Aykut Sağlam, Ni̇hal Kutlu, Hati̇ce Nar, Asim Kadioğlu

Turkish Journal of Botany

Common bean (Phaseolus vulgaris L.) cultivars (cvs) (Göynük 98, Karacaşehir 90, Şehirali 90, ES 855, and Yunus 90) were subjected to drought stress in order to assess the levels of drought tolerance through the analysis of growth parameters, leaf water potential (\psi_{leaf}), stomatal conductance (g_s), chlorophyll (chl) content, and lipid peroxidation. Significant differences were recorded among cultivars in most traits. Yunus 90 was identified as the most tolerant, and Karacaşehir 90 was the most sensitive. Furthermore, the changes in antioxidant enzyme activities, H_2O_2 content, and Photosystem II were investigated under drought stress, where Antioxidant enzyme activities increased while H_2O_2 contents …

Resistance Gene Analog Polymorphism (Rgap) Markers Co-Localize With Disease Resistance Genes And Qtl In Common Bean, Nedim Mutlu, Phillip Miklas, Dermot Coyne

Department of Agronomy and Horticulture: Faculty Publications

Resistance (R) genes containing nucleotide-binding site (NBS)-leucine rich repeats (LRR) are the most prevalent types of R gene in plants. The objective of this study was to develop PCR-based R-gene analog polymorphism (RGAP) markers for common bean (Phaseolus vulgaris L). Twenty degenerate primers were designed from the conserved kinase-1a (GVGKTT) and hydrophobic domains (GLPLAL) of known NBSLRR type R-genes and from EST databases. Sixty-six of the 100 primer combinations tested yielded polymorphism. Thirty-two RGAP markers were mapped in the BAT 93/Jalo EEP558 core mapping population for common bean. The markers mapped to 10 of 11 linkage groups with a …

Environmental Effects On Yield And Agronomic Traits Of Common Bean (Phaseolus Vulgaris L.), Tadesse Mebrahtu, Teklu Andebrhan, Ali Mohamed

Virginia Journal of Science

Common bean (Phaseolus vulgaris L.) demand is increasing with an alarming rate around the world, especially in Latin America, Africa, and Asia. Therefore, increase bean yield per hectare is the best way to meet the world demand rather than expansion of area under cultivation. The objectives of this experiment were to determine the genotypic variations for green bean and dry seed yield and magnitude of genotype x environment interaction effects on yield and yield components of common bean. Thirteen genotypes were planted during the 1992, 1994, and 1995 growing seasons. Genotypes were evaluated for green pod and seed …