Life Sciences Commons^™

Optimizing Crop Productivity And Fertility Practices In Intermediate Wheatgrass, Roberta Bianchin Rebesquini

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The intensive management associated with many annual crops often includes recurring tillage, fertilization, and pesticide applications, which contribute to environmental concerns such as water pollution and soil erosion. Intermediate wheatgrass (Thinopyrum intermedium), recognized under the trade name Kernza®, is a perennial grass that can be managed to produce grain and biomass while providing desired environmental benefits such as soil conservation and nutrient cycling. There has been limited research on best management practices and crop productivity for this alternative dual-use crop in Nebraska. A field experiment was conducted beginning in 2021 to assess nitrogen (N), phosphorus (P), and potassium (K) management …

Evaluating Drill Interseeded Cover Crop Establishment And Nitrogen Impact In Irrigated Corn, Victor De Sousa Ferreira

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The adoption of cover crops as a strategy to improve soil health and cropping systems sustainability is on the rise in the United States. PRE herbicides with soil residual activity are widely applied in corn production systems to prevent early season weed development, crop-weed competition, and yield loss. When preemergence herbicides are applied in the field, the active ingredients remain in the soil rhizosphere for a period of time, killing weed seedlings as they emerge. However, PRE herbicides can also impact the establishment of interseeded cover crops. Greenhouse bioassay was conducted to evaluate the preemergence herbicide carry-over potential to interseeded …

Macro And Micro-Nutrient Accumulation And Partitioning In Soybean Affected By Water And Nitrogen Supply, Ingrid Silva Setubal, Aderson Soares De Andrade Júnior, Silvestre Paulino Da Silva, Artenisa Cerqueira Rodrigues, Aurenívia Bonifácio, Evandro Henrique Figueiredo Moura Da Silva, Paulo Fernando De Melo Jorge Vieira, Rafael De Souza Miranda, Nicolas Cafaro La Menza, Henrique Antunes De Souza

West Central Research and Extension Center, North Platte

This study aimed to investigate the influence of water availability and nitrogen fertilization on plant growth, nutrient dynamics, and variables related to soybean crop yield. Trials were performed in Teresina, Piauí, Brazil, using randomized blocks in a split-split plot arrangement. The plots corresponded to water regimes (full and deficient), the split plots to N fertilization (0 and 1000 kg ha^-1 N-urea), and the split-split plots to harvest times of soybean plants (16, 23, 30, 37, 44, 58, 65, 79 and 86 days after emergence), with three replicates. In general, the accumulation and partitioning of nitrogen (N), phosphorus (P), potassium …

Exploring Gene Expression Patterns For Resilient Maize Lines Under Nitrogen Stress, Alice Guo

Honors Theses

Nitrogen plays a major role in the proper growth and development of maize and is therefore essential to crop production, being the most critical nutrient for achieving optimal yield. In previous field and greenhouse studies, we have found maize lines that differ in their resiliency to nitrogen stress. To identify the potential genomic regions associated with the differences in nitrogen deficiency resilience, Genome-Wide Association Studies (GWAS) were conducted. Based on a previous study, the most consistently resilient maize inbred lines within the Goodman-Buckler diversity panel have been identified as the lines A619 and A661, while the non-resilient inbred lines have …

Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen is an essential nutrient required for growth and development in plants. Insufficient nitrogen availability can reduce vegetative growth and grain yield. However, nitrogen is a costly input for farmers, is energy intensive to manufacture, and runoff of excess nitrogen fertilizer impacts water quality. Compared to its close relative, maize, sorghum has much greater resilience to nitrogen and water deficit, and heat stress, allowing sorghum to be grown with fewer inputs and on marginal land. Variation in total biomass accumulation and grain yield between sorghum accessions, as well as between nitrogen conditions, can be largely explained by differences in vegetative …

Economic And Input-Use Efficiency Of Nitrogen Management Techniques In Non-Irrigated Maize Production, Katie J. Bathke

Honors Theses

The efficiency of nitrogen (N) management has become a main concern in agricultural cropping systems for understanding the optimal N rate to help producers improve economically and reduce the exhaustion of natural resources environmentally. Nitrogen rates vary both temporally and spatially by the interactions of the soil environment and rainfall through a growing season. Thus, a site-specific approach can further optimize this variability with the understanding of the role soil variability plays in the N rate applied to a non-irrigated field. The objective of this study was to evaluate the various nitrogen treatments for their nutrient use efficiency and economic …

Nitrification Inhibitor, Nitrogen Source, And Herbicide Effects On Soil Nitrogen Transformations And Corn Yield, William Neels

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen fertilizer management continue to be challenging due to potential nitrogen losses under variable weather conditions. The objective of this was to evaluate the performance of nitrification inhibitors, nitrogen sources and herbicides on in-season nitrogen availability and agronomy indicators. A two site-year field experiment was conducted in silty clay loam soil in corn phase of the corn-soybean rotation at Central Nebraska. The treatments included three herbicide (no pre-emergence, Acuron, Resicore) and five nitrogen treatments: 1) control, 2) anhydrous ammonia, 3) anhydrous ammonia without nitrification inhibitor, 4) urea with nitrification inhibitors, and 5) urea without nitrification inhibitors. Results indicated that nitrogen …

Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan Mcpherson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Arbuscular mycorrhizal fungi (AMF) remain a vital obligate symbiont of nearly all plants. It is well established that the symbiosis between AMF and host plant improves plant nutrient acquisition, alleviates abiotic and biotic environmental stressors, defends against plant pathogens, and contributes to overall plant fitness and productivity through modification of the soil habitat. Modifications include increased soil aggregation and stability, carbon sequestration through provision of fungal wall precursors to soil organic matter (SOM) formation, and enhanced nutrient cycling in the mycorrhizosphere. The goal of this dissertation was to assess how AMF respond to nitrogen (N) fertilization regimes in maize cropping …

Post-Termination Effects Of Cover Crop Monocultures And Mixtures On Soil Inorganic Nitrogen And Microbial Communities On Two Organic Farms In Illinois, Eleanor E. Lucadamo, Ashley A. Holmes, Samuel E. Wortman, Anthony C. Yannarell

Department of Agronomy and Horticulture: Faculty Publications

Cover crops can continue to affect agricultural systems even after they have been terminated by influencing nitrogen dynamics and by altering soil microbial communities. These post-termination effects can influence soil fertility, weed pressure, and the dynamics of potential plant pathogens in the narrow window of time between cover crop termination and cash crop emergence. We evaluated the post-termination effects of 12 different spring-sown cover crop mixtures and monocultures on soil nitrogen and microbial communities on two different organic farms in Central Illinois (on Lawson silt loam soil) and Northern Illinois (on Virgil silt loam soil). In comparison to control plots …

Post-Termination Effects Of Cover Crop Monocultures And Mixtures On Soil Inorganic Nitrogen And Microbial Communities On Two Organic Farms In Illinois, Eleanor E. Lucadamo, Ashley A. Holmes, Samuel E. Wortman, Anthony C. Yannarell

Department of Agronomy and Horticulture: Faculty Publications

Cover crops can continue to affect agricultural systems even after they have been terminated by influencing nitrogen dynamics and by altering soil microbial communities. These post-termination effects can influence soil fertility, weed pressure, and the dynamics of potential plant pathogens in the narrow window of time between cover crop termination and cash crop emergence. We evaluated the post-termination effects of 12 different spring-sown cover crop mixtures and monocultures on soil nitrogen and microbial communities on two different organic farms in Central Illinois (on Lawson silt loam soil) and Northern Illinois (on Virgil silt loam soil). In comparison to control plots …

Ecosystem-Scale Biogeochemical Fluxes From Three Bioenergy Crop Candidates: How Energy Sorghum Compares To Maize And Miscanthus, Caitlin E. Moore, Adam C. Von Haden, Mark B. Burnham, Ilsa B. Kantola, Christy D. Gibson, Bethany J. Blakely, Evan C. Dracup, Michael D. Masters, Wendy H. Yang, Evan H. Delucia, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Perennial crops have been the focus of bioenergy research and development for their sustainability benefits associated with high soil carbon (C) and reduced nitrogen (N) requirements. However, perennial crops mature over several years and their sustainability benefits can be negated through land reversion. A photoperiod-sensitive energy sorghum (Sorghum bicolor) may provide an annual crop alternative more ecologically sustainable than maize (Zea mays) that can more easily integrate into crop rotations than perennials, such as miscanthus (Miscanthus × giganteus). This study presents an ecosystem-scale comparison of C, N, water and energy fluxes from energy sorghum, maize and miscanthus during a typical …

Insufficient Nitrogen Supply From Symbiotic Fixation Reduces Seasonal Crop Growth And Nitrogen Mobilization To Seed In Highly Productive Soybean Crops, Nicolas Cafaro La Menza, Juan Pablo Monzon, John L. Lindquist, Timothy J. Arkebauer, Johannes M.H. Knops, Murray Unkovich, James E. Specht, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen (N) supply can limit the yields of soybean [Glycine max (L.) Merr.] in highly productive environments. To explore the physiological mechanisms underlying this limitation, seasonal changes in N dynamics, aboveground dry matter (ADM) accumula- tion, leaf area index (LAI) and fraction of absorbed radiation (fAPAR) were compared in crops relying only on biological N2 fixation and available soil N (zero-N treatment) versus crops receiving N fertilizer (full-N treatment). Experiments were conducted in seven high-yield environments without water limitation, where crops received optimal management. In the zero-N treatment, biological N2 fixation was not sufficient to meet the N demand of …

Transforming Unmanned Aerial Vehicle (Uav) And Multispectral Sensor Into A Practical Decision Support System For Precision Nitrogen Management In Corn, Laura J. Thompson, Laila A. Puntel

Department of Agronomy and Horticulture: Faculty Publications

Determining the optimal nitrogen (N) rate in corn remains a critical issue, mainly due to unaccounted spatial (e.g., soil properties) and temporal (e.g., weather) variability. Unmanned aerial vehicles (UAVs) equipped with multispectral sensors may provide opportunities to improve N management by the timely informing of spatially variable, in-season N applications. Here, we developed a practical decision support system (DSS) to translate spatial field characteristics and normalized difference red edge (NDRE) values into an in-season N application recommendation. On-farm strip-trials were established at three sites over two years to compare farmer’s traditional N management to a split-application N management guided by …

Benchmarking Impact Of Nitrogen Inputs On Grain Yield And Environmental Performance Of Producer Fields In The Western Us Corn Belt, Fatima A.M. Tenorio, Eileen L. Mclellan, Alison J. Eagle, Kenneth G. Cassman, Daryl Andersen, Marie Krausnick, Russell Oaklund, John Thorburn, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Benchmarking crop yields against nitrogen (N) input levels can help provide opportunities to improve N ferti-lizer efficiency and reduce N losses on maize in the US Corn Belt by identifying fields most likely to benefit from improved N management practices. Here, we evaluated a large producer database that includes field-level data on yield and applied N inputs from 9280 irrigated and rainfed fields over a 7-year period (2009–2015) in Nebraska (USA). A spatial framework, based on technology extrapolation domains, was used to cluster each field into spatial units with similar climate and soil type that represent 1.3 million ha of …

Size And Density Of Upside-Down Jellyfish, Cassiopea Sp., And Their Impact On Benthic Fluxes In A Caribbean Lagoon, Chester B. Zarnoch, Noshin Hossain, Erika Fusco, Mary Alldred, Timothy J. Hoellein, Sophia Perdikaris

School of Global Integrative Studies: Faculty Publications

Anthropogenic disturbances may be increasing jellyfish populations globally. Epibenthic jellyfish are ideal organisms for studying this phenomenon due to their sessile lifestyle, broad geographic distribution, and prevalence in near-shore coastal environments. There are few studies, however, that have documented epibenthic jellyfish abundance and measured their impact on ecological processes in tropical ecosystems. In this study, the density and size of the upside-down jellyfish (Cassiopea spp.) were measured in Codrington Lagoon, Barbuda. A sediment core incubation study, with and without Cassiopea, also was performed to determine their impact on benthic oxygen and nutrient fluxes. Densities of Cassiopea were 24–168 …

Understanding Nitrogen Limitation In Soybean, Nicolas Cafaro La Menza

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Meeting soybean demand on existing cropland area for a global population of 9.7 billion people by the year 2050 requires narrowing the existing gap between average producer yield and yield potential. Soybean relies on two sources on nitrogen (N): biological N2 fixation and indigenous soil N supply. As soybean yield continues to increase, it seems critical to know if there is a yield level at which potential contribution of indigenous nitrogen sources and fixation becomes insufficient to meet crop N requirements for high yields, while still maintaining or increasing protein and oil concentration. This study evaluated N limitation across 29 …

Benchmarking On-Farm Maize Nitrogen Balance In The Western U.S. Corn Belt, Fatima Amor Tenorio

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A nitrogen (N) balance, calculated as the difference between N inputs and grain-N removal, provides an estimate of the potential N losses. We used N balance with other N-related metrics (partial factor productivity for N inputs, and yield-scaled N balance), to benchmark maize yields in relation with N input use in the US Corn Belt. We first used experimental data on grain-N concentration (GNC) to assess variation in this parameter due to biophysical and management factors. Subsequently, we used N balance and N-related metrics to benchmark yields in relation with N inputs in irrigated and rainfed fields in Nebraska using …

Effects Of Different Water And Nitrogen Regimens On Yield Of Winter Wheat Produced In Nebraska, Joseph Emory Davis

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Wheat is the 3rd most prominent crop in the USA and approximately 50% is exported annually. Nebraska wheat production is 11^th in the country, and it plays a major role in the state's agricultural economy, especially in western NE. Generally, wheat is grown under dryland conditions and the region grows much more wheat on unirrigated land than it does on irrigated. However, deficit irrigation has shown great value in producing high yielding wheat with much less water than needed for other crops. Finding new ways to leverage irrigation in wheat production may help address the need to produce food …

Metabolomics Of Sorghum Roots During Nitrogen Stress Reveals Compromised Metabolic Capacity For Salicylic Acid Biosynthesis, Amy M. Sheflin, Dawn Chiniquy, Chaohui Yuan, Emily Goren, Indrajit Kumar, Max Braud, Thomas Brutnell, Andrea L. Eveland, Susannah Tringe, Peng Liu, Stephen Kresovich, Ellen Marsh, Daniel P. Schachtman, Jessica E. Prenni

Nebraska Center for Biotechnology: Faculty and Staff Publications

Sorghum (Sorghum bicolor [L.] Moench) is the fifth most productive cereal crop worldwide with some hybrids having high biomass yield traits making it promising for sustainable, economical biofuel production. To maximize biofuel feedstock yields, a more complete understanding of metabolic responses to low nitrogen (N) will be useful for incorporation in crop improvement efforts. In this study, 10 diverse sorghum entries (including inbreds and hybrids) were field-grown under low and full N conditions and roots were sampled at two time points for metabolomics and 16S amplicon sequencing. Roots of plants grown under low N showed altered metabolic profiles at …

Approaches To Evaluating Grower Irrigation And Fertilizer Nitrogen Amount And Timing, Tsz Him Lo, Daran Rudnick, Charles Burr, Matt Stockton, Rodrigo Werle

West Central Research and Extension Center, North Platte

Diversity in the knowledge, mindset, strategies, and tools that growers use to manage irrigation and fertilizer nitrogen culminates in diversity in profitability and environmental impact among farms. As growers, academia, and industry strive together to tackle the technological and non-technological challenges impeding better irrigation and fertilizer nitrogen management, a science-based evaluation of grower input amount and timing becomes an important initial step in the process. Providing such valuable feedback to growers is a high-priority objective for the University of Nebraska-Lincoln Testing Ag Performance Solutions (UNL-TAPS) farm management competition. In this competition, each team of mostly growers made management decisions for …

Effects Of Nitrogen And Phosphorus Fertilizer And Topsoil Amendment On Native Plant Cover In Roadside Revegetation Projects, Heidi L. Hillhouse, Walter H. Schacht, Jonathan M. Soper, Carol E. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Establishing vegetation on roadsides following construction can be challenging, especially for relatively slow growing native species. Topsoil is generally removed during construction, and the surface soil following construction (“cut-slope soils”) is often compacted and low in nutrients, providing poor growing conditions for vegetation. Nebraska Department of Transportation (NDOT) protocols have historically called for nitrogen (N) and phosphorus (P) fertilization when planting roadside vegetation following construction, but these recommendations were developed for cool-season grass plantings and most current plantings use slower-establishing, native warmseason grasses that may benefit less than expected from current planting protocols. We evaluated the effects of nitrogen and …

Nutrients In The Nexus, Eric A. Davidson, Rachel L. Nifong, Richard B. Ferguson, Cheryl Palm, Deanna L. Osmond, Jill S. Baron

Department of Agronomy and Horticulture: Faculty Publications

Synthetic nitrogen (N) fertilizer has enabled modern agriculture to greatly improve human nutrition during the twentieth century, but it has also created unintended human health and environmental pollution challenges for the twentyfirst century. Averaged globally, about half of the fertilizer-N applied to farms is removed with the crops, while the other half remains in the soil or is lost from farmers’ fields, resulting in water and air pollution. As human population continues to grow and food security improves in the developing world, the dual development goals of producing more nutritious food with low pollution will require both technological and socioeconomic …

Grain Sorghum Leaf Reflectance And Nitrogen Status, Akwasi A. Abunyewa, Richard B. Ferguson, Charles S. Wortmann, Stephen C. Mason

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen deficiency is a common but readily managed constraint to grain yield. A quick and nondestructive detection of crop N status using remote sensing could be a means to increased N use efficiency. Research was conducted in a greenhouse in 2006 at the University of Nebraska-Lincoln to establish the relationship of spectral reflectance with N status in leaves of grain sorghum, to develop indices for interpretation of the results and to predict chlorophyll content. Nitrogen stress decreased chlorophyll meter reading and leaf N content, but increased leaf and canopy reflectance. The SPAD values were significantly increased by both water and …

Nitrogen And Water Effects On Canopy Sensor Measurements For Site-Specific Management Of Crops, Nicholas C. Ward

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Water and nitrogen (N) are undoubtedly the two largest agricultural inputs globally. Coupled with advances in site-specific management technology their integration into production agriculture will allow for the most efficient use these crop input resources. Active canopy sensors offer the ability to measure biophysical plant traits rapidly and make assessments about plant status. Specifically, optical sensor measurements of light reflectance assess plant N status allowing for in-season and on-the-go N recommendations and applications; while infrared thermometers (IRT) measurement of canopy temperature can be used a tool for irrigation management. To evaluate how these technologies work among different plant stress environments …

Combined Effects Of Phosphorus Nutrition And Elevated Carbon Dioxide Concentration On Chlorophyll Fluorescence, Photosynthesis, And Nutrient Efficiency Of Cotton, Shardendu K. Singh, Vangimalla R. Reddy

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

To examine the combined effects of phosphorus (P) nutrition and CO2 on photosynthesis, chlorophyll fluorescence (CF), and nutrient utilization and uptake, two controlled-environment experiments were conducted using 0.01, 0.05 and 0.20 mM external phosphate each at ambient and elevated CO2 (aCO2: 400 and eCO2: 800 mmol mol–1, respectively). The CF parameters were affected more by P nutrition than by CO2 treatment. Photoinhibition of photosystem II (PSII) was due to increased minimal CF (Fo') and decreased maximal CF (Fm'), and efficiency of energy harvesting (Fv'/Fm'). In addition, reduced electron transport rate (ETR), the quantum yield of PSII (FPSII) and CO2 assimilation …

Remote Estimation Of Nitrogen And Chlorophyll Contents In Maize At Leaf And Canopy Levels, Michael Schlemmer, Anatoly A. Gitelson, James S. Schepers, Richard B. Ferguson, Y. Peng, J. Shanahan, Donald Rundquist

Department of Agronomy and Horticulture: Faculty Publications

Leaf and canopy nitrogen (N) status relates strongly to leaf and canopy chlorophyll (Chl) content. Remote sensing is a tool that has the potential to assess N content at leaf, plant, field, regional and global scales. In this study, remote sensing techniques were applied to estimate N and Chl contents of irrigated maize (Zea mays L.) fertilized at five N rates. Leaf N and Chl contents were determined using the red-edge chlorophyll index with R2 of 0.74 and 0.94, respectively. Results showed that at the canopy level, Chl and N contents can be accurately retrieved using green and red-edge Chl …

High Yield Soybean Management: Planting Practices, Nutrient Supply, And Growth Modification, Evan Sonderegger

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Growers are constantly seeking ways to improve yield in soybean [Glycine max (L.) Merr.]. There has been much interest in the use of selected alternative practices to maximize soybean yield. These practices include planting soybean at higher than recommended seeding rates, planting soybean in narrow rows, breaking apical dominance to induce branching, application of strobilurin fungicides prophylactically to minimize disease and extend the seed filling period, the use of N fertilizer both in furrow and foliar applied, and the use of seed treatments to promote early stand establishment and health. Field studies were conducted at the University of Nebraska …

Decomposition And Nutrient Release Of Different Cover Crops In Organic Farm Systems, Jianru Shi

School of Natural Resources: Dissertations, Theses, and Student Research

Cover crops act as green manure adding organic matter to agricultural-soils. For legume green manures to be an effective nitrogen (N) source for organic farming systems, their N release must be in synchrony with crop N demand. The objectives of this study were 1) determine the decomposition rates of three common cover crops (white clover, (Trifolium repens,L ) red clover (Trifolium pratense L.) and soybean (Glycine max L) in order to determine when most N was released and its synchrony with subsequent corn crop uptake; 2) we focused on the effect of cover crops on soil …

A Review Of Cover Crops For Eastern Nebraska, Tyler Williams

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The focus of this project is two fold, first to provide a short literature review of cover crops (CC) and then discuss the results from a field experiment that was designed to evaluate the nitrogen contribution from cover crop mixes to the subsequent corn crop. The literature review will focus on the aspects of CC that are related to the field of study. Cover crops, as defined by the United States Department of Agriculture (USDA) are crops that are agronomically sound and grown for the purpose of erosion control or other objectives related to conservation or soil improvement. Cover crops …

Trait Convergence And Plasticity Among Native And Invasive Species In Resource-Poor Environments, Rebecca E. Drenovsky, Albina Khasanova, Jeremy J. James

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Premise of study: Functional trait comparisons provide a framework with which to assess invasion and invasion resistance. However, recent studies have found evidence for both trait convergence and divergence among coexisting dominant native and invasive species. Few studies have assessed how multiple stresses constrain trait values and plasticity, and no study has included direct measurements of nutrient conservation traits, which are critical to plants growing in low-resource environments.

Methods: We evaluated how nutrient and water stresses affect growth and allocation, water potential and gas exchange, and nitrogen (N) allocation and use traits among a suite of six codominant species from …