Life Sciences Commons^™

In Vitro Propagation Of Cydonia Oblonga Cv. Esme, Melekber Sülüşoğlu Durul, Tülay Korana Aktaş

Turkish Journal of Agriculture and Forestry

Quince (Cydonia oblonga) is a pome fruit belonging to the Rosaceae family and is produced in temperate climates. The popularity of quince fruit is increasing year by year. It is likely to become a highly popular fruit in the near future. Quince fruit and its leaves are rich in bioactive compounds. In addition to providing significant health benefits, different parts of the plant are an important source of raw materials, with applications in the health and pharmaceutical sectors. Micropropagation is a methodology that shortens the time required for sapling material propagation in the field and allows for the breeding of …

The Effects Of Zinc Oxide Nanoparticles Loaded With Iaa And Iba On In Vitro Rooting Ofapple Microcuttings, Shabnam Alizadeh, Hati̇ce Dumanoğlu

Turkish Journal of Agriculture and Forestry

In the micropropagation of difficult-to-root plants, the low rates of rooting cause high economic losses. The local apple (Malus domestica Borkh.) cultivar #67, which is highly resistant to fire blight disease, has been shown to have low rooting success. Nanoparticles loaded with auxins could increase the rooting success with their physicochemical properties. To increase rooting percentage, the effects of zinc oxide nanoparticles (nZnO), nZnO loaded with indole-3-acetic acid (IAA-nZnO) and indole-3-butyric acid (IBA-nZnO) at different concentrations (1.0, 2.0, 3.0, 4.0, 5.0, and 6.0 mg L-1) on in vitro rooting of microcuttings were investigated. The concentrations of auxin carried by the …

Propagation Of Two Utah Native Plants: Ceanothus Velutinus And Cercocarpus Montanus, Asmita Paudel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Among various water conservative approaches, the use of native plants in landscape, such as Ceanothus velutinus (snowbrush ceanothus) and Cercocarpus montanus (alder-leaf mountain mahogany), is attractive. Efficient propagation methods are required to allow these native species to use in water-efficient landscaping. Sexual (seed) and asexual/vegetative (cuttings and micropropagation) propagation methods were evaluated.

Seeds of both C. velutinus and C. montanus were scarified and/or stratified and treated with gibberellic acid (GA₃) to break dormancy. The results showed hot water scarification and 2-3 months of stratification effectively broke the dormancy of C. velutinus seeds, and stratification for 2-3 months was …

Development Of An In Vitro Micropropagation Protocol For Myrobalan 29c Rootstock, Murat Güney

Turkish Journal of Agriculture and Forestry

Stone fruits are well known for their high nutritional value. Therefore, in horticulture, the micropropagation of suitable rootstocks is vital for their cultivation. The aim of the present study was to improve the micropropagation protocol of Myrobalan 29C (Prunus cerasifera Ehrh.) rootstock using shoot-tip culture. Murashige and Skoog (MS) basal medium containing 2 mg L-1 6-benzylaminopurine (BAP) and 0.05 mg L-1 gibberellic acid (GA3) resulted in the highest number (14.3) of multiple shoots. However, a greater shoot length (2.0 cm) was attained when GA3 was excluded from the MS medium and the concentration of BAP was reduced to 1 mg …

Influence Of Micropropagation On The Ontogenetic Phases Of Paulownia, Michael Pezzaniti

Theses: Doctorates and Masters

The aim of this research was to examine whether the method of micropropagation and tissue source affects the early growth and development of Paulownia in the first six months following transfer from tissue culture and establishment in soil. This tree species was chosen as it is a fast growing, short-rotation timber tree and able to adapt successfully to new environments. It is easily established in vitro and has been micropropagated using a range of different techniques. Three methods of micropropagation were chosen: callus regeneration, somatic embryogenesis and the third method was inducing root suckers in vitro. The third method …

Accumulation Of Phenolics In Natural And Micropropagated Plantlets Of Thymus Pseudopulegioides Klokov & Des.-Shost. With Their Antioxidant Potentials, Mustafa Günaydin, Abdul Hafeez Laghari, Ersan Bektaş, Münevver Sökmen, Atalay Sökmen

Turkish Journal of Biology

Thymus pseudopulegioides plantlets were propagated in vitro via direct organogenesis by using Murashige and Skoog (MS) media containing kinetin, thidiazuron, and 6-benzyladenine (BA) individually. Methanol extracts obtained both from plantlets and wild plants were analyzed for their total phenolics and flavonoid contents, then quantified by HPLC. The highest total phenolic (8.83 mg/g as gallic acid equivalent) and total flavonoid (0.92 mg/mL as rutin equivalent) values were from the MS media supplemented with 1.0 mg/L kinetin and 0.5 mg/L BA, respectively. The plantlets grown in those media also showed remarkable antioxidant activities with an IC50 value of 4.77 \mug/mL in DPPH …

In Vitro Production Protocol Of Vaccinium Uliginosum L. (Bog Bilberry)Growing In The Turkish Flora, Mustafa Cüce, Atalay Sökmen

Turkish Journal of Agriculture and Forestry

This novel approach was designed to increase the production capacity of Vaccinium uliginosum L. via direct organogenesis. Lateral buds containing one or two leaves were initially cultured in McCown woody plant medium (WPM), Anderson's rhododendron medium, and Murashige and Skoog basal media, each supplemented with zeatin/indole-3-butyric acid (IBA) and zeatin/naphthalene acetic acid (NAA) (1.0/0.1 mg L-1). WPM containing the zeatin/IBA combination was the most effective as the basal medium. Various plant growth regulators (PGRs) were then investigated for the best shoot multiplication, and zeatin was found to be most favorable PGR in all cases. The highest shoot length and shoot …

Enhancement Of Direct Shoot Regeneration And Determination Of Bioactivesecondary Metabolites In Leaves Of Galega Officinalis L., Fatma Pehli̇van Karakaş, Günce Ci̇ngöz, Arzu Türker

Turkish Journal of Biology

Galega officinalis L. is a medicinal plant being used as a galactagogue and an antidiabetic in folk medicine. A new effective micropropagation protocol was established from leaf, stem, node, and root explants of G. officinalis for in vitro plant regeneration and callus induction systems. Murashige and Skoog (MS) medium supplemented with 55 various concentrations and 4 different combinations [benzyl adenine (BA)/naphthalene acetic acid (NAA), BA/indole-3-acetic acid (IAA), thidiazuron (TDZ)/indole-3-butyric acid (IBA), and TDZ/IAA] of plant growth regulators was used for the regeneration systems. The regenerated shoots were observed on only nodal explants through direct organogenesis at the 35th day. The …

Advancement In Protocol For In Vitro Seed Germination, Regeneration, Bulblet Maturation, And Acclimatization Of Fritillaria Persica, Derya Çakmak, Cuma Karaoğlu, Muhammad Aasim, Cengi̇z Sancak, Sebahatti̇n Özcan

Turkish Journal of Biology

The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening, and acclimatization of Persian lily (Fritillaria persica). Seed germination rate was recorded as 86.7% and 96.7% after 2 and 3 months of cold treatment at 4 °C, respectively. Bulblet explants taken from a germination experiment were cultured on Murashige and Skoog (MS) medium supplemented with 0.5-2.0 mg/L thidiazuron (TDZ). Maximum bulblet regeneration frequency and bulblets per explants were achieved on a medium containing 2.0 mg/L TDZ. Leaf disc and leaf scale explants isolated from germinated seedlings were also cultured on MS medium containing 2-6 …

Rejuvenation Of Mature Lentisk By Micrografting And Evaluation Of Genetic Stability, Ahmet Onay, Engi̇n Ti̇lkat, Veysel Süzerer, Özge Karakaş Meti̇n, Yelda Özden Çi̇ftçi̇, Fati̇h Mehmet Kilinç, İbrahi̇m Koç, Muhammet Şaki̇roğlu, Hakan Yildirim, Ahu Altinkut Uncuoğlu, Nazan Çalar, Ömer Faruk Akdemi̇r

Turkish Journal of Biology

An in vitro propagation method was established for both male and female genotypes of lentisk using actively growing shoot tips derived from forcefully lignified shoots. The effects of growth regulators on in vitro morphogenesis were investigated. Since rooting of the regenerated shoots for both genotypes was not achieved, an in vitro micrografting method was developed for the production of plantlets. Moreover, genetic stability of 3-, 6-, and 24-times subcultured clones of both genotypes was assessed and compared with the mother plants using fluorescent-based amplified fragment length polymorphism (AFLP) analysis. The set of main plants and the different subcultured clones were …

Effect Of The Ethylene Inhibitors Silver Nitrate, Silver Sulfate, And Cobalt Chloride On Micropropagation And Biochemical Parameters In The Cherryrootstocks Cab-6p And Gisela 6, Virginia Sarropoulou, Kortessa Dimassi-Theriou, Ioannis Therios

Turkish Journal of Biology

In the present study, the effects of three ethylene inhibitors, silver nitrate (AgNO3), silver sulfate (Ag2SO4), and cobalt chloride (CoCl2), on the morphogenic and biochemical responses in CAB-6P and Gisela 6 cherry rootstocks were investigated. In both rootstocks, AgNO3 and Ag2SO4 promoted shoot regeneration while CoCl2 had such an effect only in Gisela 6. The rooting percentage of CAB-6P and Gisela 6 explants was diminished with AgNO3, Ag2SO4, and CoCl2. AgNO3 increased root number (40 μM) and root length (50 μM) in the CAB-6P rootstock while it had an inhibitory effect in Gisela 6. Ag2SO4 and CoCl2 enhanced root length …

In Vitro Seed Germination, Plantlet Growth, Tuberization, And Syntheticseed Production Of Serapias Vomeracea (Burm.F.) Briq., Ersan Bektaş, Atalay Sökmen

Turkish Journal of Botany

Orchids are considered recalcitrant plants in in vitro propagation. Due to the lack of appropriate micropropagation techniques for mass production and damage to their ecological distribution posed by local gatherers, these species are threatened with extinction, including Serapias vomeracea (Burm.f.) Briq. In this research, we put forward a complete micropropagation method covering in vitro micropropagation, synthetic seed formation, germination in soil, and acclimatization to ambient conditions. To the best of our knowledge this is the first report of successful synthetic seed formation and germination of S. vomeracea. Initially, seeds were germinated in different culture media and also media supplemented with …

Physicochemical And Spectroscopic Characterization Of Biofield Energy Treated Gerbera Multiplication Medium, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

Mahendra Kumar Trivedi

Effect Of A Biofield Treatment On Plant Growth And Adaptation (Benth.), Gopal Nayak, Nandini Altekar

Mahendra Kumar Trivedi

Micropropagation Of Vaccinium Myrtillus L. (Bilberry) Naturally Growingin The Turkish Flora, Mustafa Cüce, Atalay Sökmen

Turkish Journal of Biology

This study was designed to micropropagate Vaccinium myrtillus L., a naturally growing bilberry, in the Turkish flora. In order to determine the most effective basal medium, lateral buds were initially selected as explants, which were then individually cultured in Murashige and Skoog medium, Anderson's rhododendron medium, and McCown's woody plant medium (WPM) supplemented with 1.0 mg L-1 zeatin in combination with either 0.1 mg L-1 indole-3-butyric acid (IBA) or 0.1 mg L-1 \alpha-naphthalene acetic acid. WPM, supplemented with zeatin and IBA, was found to be the most effective basal medium tested. As shoot regeneration ability highly depends on the medium …

Effects Of Benzyladenine Purine And Its Interaction With Polyamines On Growth Of Spathoglottis Plicata Plbs, Zaliyatun Akhma Mat Yasin, Maziah Mahmood, Noor Azmi Shaharuddin

Turkish Journal of Botany

Spathoglottis plicata is highly sought after by florists and orchid growers due to its unique structure and colors. However, this orchid is a slow-growing type and thus the planting materials are acutely limited. Therefore, there is a need to improve its multiplication efficiency and speed up its growth in order to meet the market demand. Benzyladenine purine (BAP) has been widely used to improve plantlet multiplication in plant cultures. Polyamines have also been reported to improve the growth and development of in vitro cultures. The objective of this study was to investigate the effects of BAP and different polyamines on …

Effects Of Culture Media And Plant Growth Regulators On Micropropagation Of Willow (Salix Matsudana ‘Golden Spiral’) And Hazelnut (Corylus Colurna ‘Te Terra Red), Dongxue Shi

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The branches and leaves of Salix matsudana ‘Golden Spiral’ , willow, grow in a twisted manner, which makes it an important bonsai plant. Its mature stems have potential for the woody cut floral industry. Corylus colurna ‘ Te Terra Red’, hazelnut, distinguished by red or purple leaves in the spring, has potential ornamental value in the horticulture industry for landscape use. Micropropagation of these two plants could provide more and healthier plantlets for rapid commercial scale-up by the nursery industry. Nas and Read Medium (NRM) is a newer medium developed specifically for hazelnut species by Nas and Read based on …

Unitized, Nonselective Cutting Of In Vitro Watermelon, Jeff W. Adelberg, Y Alper, R E. Young, B B. Rhodes

Jeffrey W Adelberg

Unitized, nonselective mass cutting of Stage II Citrullus lanatus cv. Charlee (watermelon) plant tissue cultures has been achieved with various configurations and sizes of wire cutter devices. Time studies revealed that the wire cutters increased the productivity of the cutting function over conventional scalpel and forceps by a factor of 14, Total transfer productivity, including the manual functions of removing tissue from an initial culture vessel and filling (sorting and placing cut tissue segments) new vessels, was increased by a factor of 1,8, The square grid-type cutting devices yielded from 48 to 59% as many viable bud clusters per culture …

Approaching Mechanization Of Plant Micropropagation, Jeffrey Adelberg, R E. Young, A Hale, N D. Camper

Jeffrey W Adelberg

Investigations of materials and methods for growing plant tissue in a continual-flow, liquid nutrient medium as an alternative to semisolid agar medium have been made. Enhanced growth of plant tissue on microporouspolypropylene membranes floating on a liquid nutrient has been demonstrated. Moreover, in vitro plantlets on the microporous membrane are free from entanglement with the support matrix and readily available to mechanizedhandling. Trained growth of plantlets through polypropylene netting shows potential for mechanization by mass handling (separation, singulation, and transfer) of plant tissue cultures.

Mass Handling Of Watermelon Microcuttings, Jeffrey Adelberg, Y Alper, R E. Young, B B. Rhodes

Jeffrey W Adelberg

Modifications were made in the configurations of the unitizing, nonselective wire cutters used by Alper et al.(1992) for mass cuttings of Stage II Citrullus lanatus cv. Charlee (watermelon) plant tissue cultures to further enhance productivity. Mounting the cutter in an inverted position over the receiving vessel eliminated time required for filling. This cut-and-dump technique became 4.8 times more productive for the total transfer process than the conventional scalpel and forceps technique when both time and yield of cut segments with visible buds were considered. A concept for growing fewer, larger tissue clusters per vessel in mini-trays with orienting cells and …

Micropropagation Of Erodium Sibthorpianum Subsp. Sibthorpianum, An Endemic Threatened Species Of Uludağ Mountain (Bursa-Turkey), Betül Akin, İsmai̇l Kocaçalişkan, Gürcan Güleryüz

Turkish Journal of Botany

Uludağ Mountain is one of the Important Plant Areas of Turkey. Its native plant species are threatened by various human and environmental factors. In this study, 2 different micropropagation techniques, with Murashige Skoog (MS) and modified MS (ModMS) media, were used for Erodium sibthorpianum Boiss. subsp. sibthorpianum. Mineral composition was developed according to the plant requirements. Shoot tips were cultured on these media. Seedlings, obtained from in vitro germinated seeds, were used as explant sources in micropropagation studies. The highest percentage of shoot induction (11.33 shoots/explant) was determined in 1 ppm 6-benzyl amino purine and plant growth regulator-free MS medium …

In Vitro Regeneration And Conservation Of The Lentisk (Pistacia Lentiscus L.), İbrahi̇m Koç, Ahmet Onay, Yelda Özden Çi̇ftçi̇

Turkish Journal of Biology

Pistacia lentiscus L. (lentisk or mastic tree) is an economically important member of the genus Pistacia due to its valuable mastic resin. Shoot tips and nodal segments were used as explant sources from in vitro-germinated seeds of lentisk. Shoot tips were found to be suitable for multiple shoot formation. Thereafter, the influences of different growth regulators [N6-benzyladenine (BA), gibberellic acid (GA_3), naphthalene acetic acid (NAA), or jasmonic acid (JA)] together with various elicitors [silver nitrate (AgNO_3) or phloroglucinol (PG)] were assessed to develop efficient micropropagation protocol. Our results showed that the combination of all tested concentrations of GA_3 with 1.0 …

Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati

Rajesh Pati

Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for …

In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati

Rajesh Pati

Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).

Verticordia Micropropagation Through Direct Ex Vitro Rooting, Belinda Delaney

Theses: Doctorates and Masters

The objective of this study was to improve the existing shoot multiplication protocol for Verticordia grandis (McComb, Arthur & Newll, 1986; Newell, Growns & McComb, 2005) and to investigate and establish reliable root induction and acclimatisation protocols to enhance survival of micropropagated plantlets. It was envisaged that these protocols would be successful in micropropagation, growth and survival of different V. grandis clones and possibly applicable to other Verticordia species.

The elongation of in vitro Verticordia shoots on multiplication media was improved by reducing the concentration of BAP from 1μM to 0.25 μM, which resulted in a more uniform shoot length …

Shortcut To Long-Distance Developing Of A Tissue Culture Medium: Micropropagation Of Mature Almond Cultivars As A Case Study, Mehmet Nuri̇ Nas, Yüksel Bölek, Nevzat Sevgi̇n

Turkish Journal of Botany

Culture medium development methods are time-consuming, laborious, and require a large amount of experimental materials. The Nas hypothesis for the development of a micropropagation medium offers a less time-consuming and a less laborious approach. According to this hypothesis, the composition of a culture medium for a particular species should resemble the seed composition. In accordance with this hypothesis, first, an almond culture medium [Nas Almond Medium (NAM)] was developed based on almond kernel composition. Then, using mature tissues of almond cultivars, the growth of cultures on NAM was compared to that on Murashige and Skoog medium (MS), Woody Plant Medium …

Antioxidant Activity Of In Vitro Propagated Stevia Rebaudiana Bertoni Plants Of Different Origins, Ely Zayova, Ira Stancheva, Maria Geneva, Maria Petrova, Lyudmila Dimitrova

Turkish Journal of Biology

An efficient in vitro protocol for propagation of Stevia rebaudiana Bertoni is described. Multiple shoots were induced in vitro from shoot tip and nodal segments on Murashige and Skoog medium containing 6-benzylaminopurine, zeatin, or thidiazuron alone and in combination with naphthalene acetic acid or indole-3-acetic acid. A high frequency of shoot induction as well as maximum number of shoots per shoot tip explant was observed on Murashige and Skoog medium supplemented with 6-benzylaminopurine (1.0 mg L^{-1}) alone and combined with indole-3-acetic acid (0.1 mg L^{-1}). For root induction, in vitro shoots were transferred to rooting media containing naphthalene acetic acid, …

Comparative Studies On The Competence Of Axillary Shoot Regeneration On Unsliced And Longitudinally Sliced Cotyledon Nodes Of Vigna Unguiculata, Muhammad Aasim, Sancar Fati̇h Özcan, Khalid Mahmood Khawar, Sebahatti̇n Özcan

Turkish Journal of Botany

Vigna unguiculata is an important food legume crop in the semiarid tropics. It suffers from a host of agricultural constraints including damage due to diseases and pests. Judicious application of biotechnological methods can lead to considerable improvement in this important crop. Shoot regeneration from unsliced and longitudinally sliced cotyledon node explants obtained from 3-day-old to 5-day-old seedlings grown in vitro was achieved on Murashige and Skoog (MS) medium containing 1.11, 2.22, 3.33, and 4.44 \muM benzylaminopurine (BA) supplemented with 1.0 mg/L polyvinylpyrrolidone and 500 mg/L bacteriostatic Augmentin. Callus induction was recorded in all culture media on both explants. The shoot …

In Vitro Plant Regeneration Via Petiole Callus Of Viola Patrinii And Genetic Fidelity Assessment Using Rapd Markers, Gururaj Chalageri, Uddagiri Venkanna Babu

Turkish Journal of Botany

Viola patrinii DC. (Violaceae) petiole explants were used for inducing calli. Significant callus proliferation was observed on MS medium supplemented with 16.12 \muM NAA and 13.33 \muM BA. Shoot regeneration was achieved upon transferring the green friable calli to MS medium with a 2-fold dilution of potassium dihydrogen phosphate supplemented with 23.25 \muM Kn and 2.68 \muM NAA. Maximum shoot regeneration was achieved in 4 weeks. Multiple shoots were separated and further cultured in half-strength MS medium supplemented with 9.85 \muM IBA with 2% sucrose. Profuse root development was observed after 20-25 days of culturing. Regenerated plants were successfully transferred …

Magnolia : Polyploidy, Genome Size, And Refinement Of Protocols For Micropropagation, James Parris

All Theses

The genus Magnolia includes over 250 species that range in ploidy level from diploid to hexaploid. Although there is basic information on ploidy levels of various species, sampling is limited with specific cultivars and hybrids. The objective of this research was to determine relative genome sizes and relationships to ploidy levels among a diverse collection of species, hybrids, and cultivars using flow cytometry. Nuclei were extracted, stained with 4’, 6–diamidino–2–phenylindole (DAPI), and analyzed using a flow cytometer. Relative genome sizes were determined using Pisum sativum as the reference genome. Genome size was calibrated with ploidy level for species with documented …