Life Sciences Commons^™

Plant Sentience: Getting To The Roots Of The Problem, Krzysztof Dolega, Savannah Siekierski, Axel Cleeremans

Animal Sentience

Segundo-Ortin’s (2023) target article invites us to consider the possibility that plants can experience subjectively felt states. We discuss this speculation on the basis of the functional neurobiology of consciousness. We suggest that demonstrating plant sentience would require that we identify not only behaviors analogous to those exhibited by sentient creatures, but also the functional analogues of the mechanisms causing such behaviors. The lack of clear evidence for any kind of integration between self-states, self-movement, environmental states, memory, or affective communication within plants suggests that plant sentience remains an admittedly fascinating, but ultimately merely provocative speculation.

Anti-Inflammatory Activity Of Cili Extracts And Its Therapeutic Effect On Ulcerative Colitis Model Of Mice, Wang Li, Li Li-Lang, Li Qi-Ji, Wang Yu, Yang Xiao-Sheng

Food and Machinery

Objective: This study aimed to investigate the effect of Cili extracts in the treatment of enteritis and diarrhea. Methods: The RAW 264.7 cells were treated with different concentrations (1.25, 2.50, 5.00, 10.00, 20.00 μg/mL) of Cili extracts, and then the cell viability was determined by MTT method. RAW264.7 cells were treated with different concentrations of Cili extracts, and then lipopolysaccharide (LPS) was used to induce the cell inflammation model. The content of NO in cell culture supernatant was detected by Griess method. The UC mice model was induced by 3% DSS. The changes of signs during the modeling and drug …

Research Progress On The Mechanism Of Dietary Fiber In Regulating Blood Sugar Of Type 2 Diabetes, Yan-Feng Wang, Xi-Hong Yang, Jun-Han Cao, Chang-Feng Qu, Li-Ping Zhang, Jin-Lai Miao, Wan-Cui Xie

Food and Machinery

This article mainly reviews the hypoglycemic effect of dietary fiber on type 2 diabetes-including improving insulin resistance, regulating glucose and lipid metabolism disorders, improving oxidative stress, regulating intestinal flora and stabilizing postprandial blood glucose-delaying glucose absorption and inhibition The digestive enzyme activity was systematically analyzed and summarized from the level of action mechanism, with a view to providing more theoretical basis and reference for the application of dietary fiber in reducing and stabilizing blood glucose of type 2 diabetes.

Establishment And Improvement Of Safety Supervision Mechanism Of Online Take-Out Food, Yong-Gang Wang

Food and Machinery

This paper analyzes the current situation and types of supervision mechanism of online takeout food safety, analyzes the reasons of the problems existing in the mechanism, and perfects the feasibility of China's online takeout food safety supervision mechanism.。

Biochemistry Of Trehalose Accumulation In The Spring Field Cricket, Gryllus Veletis, Alyssa R. Stephens

Electronic Thesis and Dissertation Repository

The freeze tolerant spring field cricket, Gryllus veletis, accumulates trehalose in the blood and tissues during cold acclimation. Trehalose is the main blood sugar in insects, thus its blood concentration is tightly regulated, and trehalose is readily metabolized. How do crickets modify their metabolism to accumulate trehalose in their hemolymph and tissues? I hypothesized that trehalose production, transport, and consumption were modified during the cold acclimation to facilitate trehalose accumulation. Trehalose and the trehalose-specific transporter, TRET-1, are distributed among all tissues, and trehalose accumulates in the hemolymph, fat body, Malpighian tubules, and gut. Trehalose production increases during cold acclimation …

Mechanisms Of Voltage-Gated Sodium Channel (Nav1.5) Regulation By Intracellular Fgfs, Paweorn Angsutararux

McKelvey School of Engineering Theses & Dissertations

Voltage-gated sodium channels (NaV) conduct the inward current responsible for the initiation and propagation of the electrical signals in myocytes and neurons, known as action potentials (AP). Precise regulation of cardiac NaV1.5 opening and closing is essential for maintaining a normal heart beat. A disruption of NaV1.5 function, especially of its inactivation after opening, results in inherited cardiac arrhythmias such as Long QT Type 3 (LQT3) syndrome. This pathology is caused by an increase in the late INa that enters myocytes later in the AP and prolongs its duration. Late INa is also enhanced in acquired diseases such as heart …

Mechanisms Of Chloroperoxidases-Catalyzed Enantioselective Transformations From Spectroscopic And X-Ray Crystallographic Studies Of Enzyme-Substrate Complexes, Xiaoqing Tang

FIU Electronic Theses and Dissertations

The chloroperoxidase secreted from Caldariomyces fumago catalyzes broad spectrum of reactions. The crystallography combined with X-ray diffraction analysis was conducted to reveal recombinant CPO expressed in a modified Aspergillus niger system. Our results indicated that despite functional similarities with wild type CPO, recombinant CPO is over glycosylated with more mannose. Besides, ten iodide ion binding sites were identified in rCPO and six of them were found to be well superimposed on previously reported structure of the wild type CPO. Therefore, recombinant CPO shares almost the same structure with wild type CPO, and the Aspergillus niger is a potential system for …

Dual Control Of One Component Signaling: Mechanistic And Structural Insights Into El222 Active States, Uthama Phani R. Edupuganti

Dissertations, Theses, and Capstone Projects

Photoreceptors play a crucial role in signal transduction as specialized proteins which sense light as environmental stimuli and transduce the signal to control of downstream functions. Here we focus our attention on one class of these proteins, the Light-Oxygen-Voltage (LOV) domain, which is sensitive to blue light via an internally-bound flavin chromophore. Since their initial discovery in plant phototropins, many details of their photochemistry, chromophore interactions, and use with a diverse set of functional effectors have been described. However, several key details, especially a comprehensive understanding of signaling mechanism and its regulation, still remain elusive due in part to the …

Study On Wind‐Sand Defending Mechanism Of Single Shrub, Tingting Yang, Xinhong Wu, Guozheng Yao, Peng Li

IGC Proceedings (1997-2023)

No abstract provided.

Protonic Capacitor: Elucidating The Biological Significance Of Mitochondrial Cristae Formation, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

For decades, it was not entirely clear why mitochondria develop cristae? The work employing the transmembrane-electrostatic proton localization theory reported here has now provided a clear answer to this fundamental question. Surprisingly, the transmembrane-electrostatically localized proton concentration at a curved mitochondrial crista tip can be significantly higher than that at the relatively flat membrane plane regions where the proton-pumping respiratory supercomplexes are situated. The biological significance for mitochondrial cristae has now, for the first time, been elucidated at a protonic bioenergetics level: 1) The formation of cristae creates more mitochondrial inner membrane surface area and thus more protonic capacitance for …

Experimental Psychology Meets Behavioral Ecology: What Laboratory Studies Of Learning Polymorphisms Mean For Learning Under Natural Conditions, And Vice Versa, Brian H. Smith, Chelsea N. Cook

Biological Sciences Faculty Research and Publications

Behavior genetics, and specifically the study of learning and memory, has benefitted immensely from the development of powerful forward- and reverse-genetic methods for investigating the relationships between genes and behavior. Application of these methods in controlled laboratory settings has led to insights into gene–behavior relationships. In this perspective article, we argue that the field is now poised to make significant inroads into understanding the adaptive value of heritable variation in behavior in natural populations. Studies of natural variation with several species, in particular, are now in a position to complement laboratory studies of mechanisms, and sometimes this work can lead …

Biological Activities Of Theaflavin-3,3’-Digallate And Its Mechanism Of Action, Lei Shi-Cheng, Sun Da-Li, Wang Ya-Jie, Zhou Yan, Zhang Qing-Hai

Food and Machinery

This review summarized the recent researches on the bioactivity of theaflavin-3,3’-digallate (TFDG) and its action mechanisms, including anti-oxidation, anti-cancer, anti-inflammatory, anti-viral as well as anti-metabolic syndrome. Meanwhile, the perspective of such research was discussed, so as to promote the further studies of red tea resources and the exploitation of health products.

Synergistic Anti-Inflammatory Effects And Mechanisms Of Combined Phytochemicals, Lijuan Zhang, Carlos Virgous, Hongwei Si

Human Sciences Faculty Research

The anti-inflammatory effects of phytochemicals, bioactive components from plants having health benefits, have been heavily investigated in the last several decades. However, the gap between the high dosage demands (μM) of phytochemicals in vitro studies and the low bioavailability (nM) of most phytochemicals after consuming relevant foods/supplements in humans undermines the application of these phytochemicals in the prevention of chronic inflammation and its related chronic diseases in humans. One of the approaches to bridging this gap is to combine two or more phytochemicals/foods to synergistically prevent chronic inflammation. While increasing combinations of phytochemicals on anti-inflammation studies have been reported, there …

Electrostatically Localized Proton Bioenergetics: Better Understanding Membrane Potential, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

In Mitchell's chemiosmotic theory, membrane potential Δψ was given as the electric potential difference across the membrane. However, its physical origin for membrane potential Δψ was not well explained. Using the Lee proton electrostatic localization model with a newly formulated equation for protonic motive force (pmf) that takes electrostatically localized protons into account, membrane potential has now been better understood as the voltage difference contributed by the localized surface charge density ([H-⁺_L] + ⁿΣ _i=1 [M⁽ⁱ⁺⁾_L]) at the liquid-membrane interface as in an electrostatically localized protons/cations-membrane-anions capacitor. That is, the origin of membrane …

Catalytic Mechanism For The Conversion Of Salicylate Into Catechol By The Flavin-Dependent Monooxygenase Salicylate Hydroxylase, Débora M. A. Costa, Stefanya V. Gómez, Simara S. De Araújo, Mozart S. Pereira, Rosemeire B. Alves, Denize C. Favaro, Alvan C. Hengge, Ronaldo A. P. Nagem, Tiago A. S. Brandão

Chemistry and Biochemistry Faculty Publications

Salicylate hydroxylase (NahG) is a flavin-dependent monooxygenase that catalyzes the decarboxylative hydroxylation of salicylate into catechol in the naphthalene degradation pathway in Pseudomonas putida G7. We explored the mechanism of action of this enzyme in detail using a combination of structural and biophysical methods. NahG shares many structural and mechanistic features with other versatile flavin-dependent monooxygenases, with potential biocatalytic applications. The crystal structure at 2.0 Å resolution for the apo form of NahG adds a new snapshot preceding the FAD binding in flavin-dependent monooxygenases. The k_cat/K_m for the salicylate reaction catalyzed by the holo form is …

Freezing Of Gait: Mechanisms, Mechanics, And Management, Peter S. Myers

Arts & Sciences Electronic Theses and Dissertations

Parkinson disease (PD) is a neurodegenerative disease with multiple motor and non-motor symptoms, including postural instability, gait impairments, and cognitive deficits. More than 50% of individuals with PD experience a symptom called freezing of gait (FOG), described as a transient inability to take another step forward. Individuals with PD who experience FOG (freezers) have further postural, gait, and cognitive impairments compared to individuals with PD without FOG (non-freezers). While degeneration of the dopaminergic neurons in the substantia nigra is accepted as the primary etiology of the disease, research shows that the disease has a global impact on the brain, accounting …

Multiscale Investigation Of The Behavior Of Heart Valve Interstitial Cells In Response To Pathological Shape And Mechanical Stimulation, Ngoc Thien Lam

Graduate Theses and Dissertations

Our works aim to provide an insight into how aortic valve interstitial cells (VICs) respond to pathological shape and mechanical stimulation as well as the potential signaling pathway that mediates these responses, using a multiscale approach. A single cell model was developed to investigate the effect of altered shape on valve cell function as valve cells were reported to significantly deform during the cardiac cycle. Single VICs were controlled to take on features with different width-to-length aspect ratios that corresponded to the steady-state shapes adopted by VICs when stretched to 0%, 10% and 20%, respectively. It appeared that single VIC …

Comparison Between The Structure-Function Relationship In The Wild Type Gαi1 Protein And Its Oncogenic Mutant, Jesse Lee Goossens

Dissertations

Many signal transduction pathways are regulated by guanine nucleotide-binding (G?) proteins, which function as molecular switches fluctuating between active and inactive conformations. Proper function depends on three flexible switch regions that are involved in the relatively slow hydrolysis of GTP. Deep sequencing studies have found mutations in the GNAS and GNAI1 genes involved in tumorigenesis, among which include a mutation corresponding to a highly conserved arginine residue in the switch II region. A mutation in GNAI1 encoding an R208Q change in G?i1 has been linked to intestinal cancers. We investigated the molecular basis of oncogenesis of this mutant by studying …

The Mechanism Of Rna Interference In Arthropods, June-Sun Yoon

Theses and Dissertations--Entomology

RNA interference (RNAi) is a useful reverse genetics tool for investigation of gene function as well as for practical applications in many fields including medicine and agriculture. Due to the variability in RNAi efficiency, RNAi-based methods are currently being developed for controlling only coleopteran insects which are known to be amenable to RNAi. The first chapter of my thesis includes findings from research to investigate what are the factors that make coleopteran insects relatively more efficient in RNAi. I used Colorado potato beetle (CPB), Leptinotarsa decemlineata and its cell line (Lepd-SL1) as study models to identify genes that play key …

Investigation Into The Mechanism Of Ion Transport In Rio Negro Characiformes, Vineza D. Reduta

Undergraduate Honors Theses

Four fish species within the Characiform Order [(Rosy tetras (Hyphessobrycon rosaceus), Serpae tetras (Hyphessobrycon eques), Emperor tetras (Nematobrycon palmeri) and Penguin tetras (Thayeria obliqua)] are native to the ion-poor, acidic waters of the Rio Negro. In this paper, we focus on Wood’s revised Na⁺/NH₄⁺ exchange model to gain better insight into the mechanisms of ion transport within the Characids. We exposed each species to two experimental treatments: (i) 1mM ammonia (high external ammonia [HEA]), to test coupling of Na⁺ uptake and ammonia excretion, and (ii) 100μM Acetazolamide …

Mechanism Of Substrate Reduction By Nitrogenase, Nimesh Khadka

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nitrogen (N) is a vital element of life and the main component of chemical fertilizer. The industrial Haber-Bosch process fulfills the demands of today’s nitrogen need and is therefore considered as one of the major scientific breakthroughs of the last century. However, the Haber-Bosch process operates at very high temperature and pressure, and requires fossils fuels to drive the reaction, making it an energy expensive process. The energy demand for this process accounts for almost 3% of the total global energy consumption. In addition, the rapid population growth, economic development and depletion of limited non-renewable fossil fuels have already created …

Carisbamate Blockade Of T-Type Voltage-Gated Calcium Channels, Do Young Kim, Fang-Xiong Zhang, Stan T. Nakanishi, Timothy Mettler, Ik-Hyun Cho, Younghee Ahn, Florian Hiess, Lina Chen, Patrick G. Sullivan, S. R. Wayne Chen, Gerald W. Zamponi, Jong M. Rho

Spinal Cord and Brain Injury Research Center Faculty Publications

Objectives

Carisbamate (CRS) is a novel monocarbamate compound that possesses antiseizure and neuroprotective properties. However, the mechanisms underlying these actions remain unclear. Here, we tested both direct and indirect effects of CRS on several cellular systems that regulate intracellular calcium concentration [Ca²⁺]_i.

Methods

We used a combination of cellular electrophysiologic techniques, as well as cell viability, Store Overload‐Induced Calcium Release (SOICR), and mitochondrial functional assays to determine whether CRS might affect [Ca²⁺]_i levels through actions on the endoplasmic reticulum (ER), mitochondria, and/or T‐type voltage‐gated Ca²⁺ channels.

Results

In CA3 pyramidal neurons, kainic …

Mechanistic Insights Into The Radical S-Adenosyl L-Methionine Enzyme Mftc, Bulat Khaliullin

Electronic Theses and Dissertations

Mycofactocin is a putative peptide-derived redox cofactor in Mycobacterium family. Its putative biosynthetic pathway is encoded by the operon mftABCDEF. The initial step of this pathway is a posttranslational modification of a peptide precursor MftA, which is catalyzed by MftC enzyme. This modification only occurs in the presence of chaperone MftB. Here, we demonstrate that MftC is a radical S-adenosyl L-methionine (SAM) enzyme and we examine its catalytic mechanism. We show that the modification of MftA requires two equivalents of SAM and is implemented in two steps: (i) the decarboxylation of a C-terminal tyrosine, resulting in formation of an …

Increased Productivity In Wet Years Drives A Decline In Ecosystem Stability With Nitrogen Additions In Arid Grasslands, Junfeng Wang, Johannes M.H. Knops, Chad E. Brassil, Chunsheng Mu

School of Biological Sciences: Faculty Publications

Adding nutrients to nutrient-limited ecosystems typically lowers plant diversity and decreases species asynchrony. Both, in turn, decrease the stability of productivity in the response to negative climate fluctuations such as droughts. However, most classic studies examining stability have been done in relatively wet grasslands dominated by perennial grasses. We examined how nutrient additions influence the stability of productivity to rainfall variability in an arid grassland with a mix of perennial and annual species. Of the nutrients, only nitrogen increased productivity, and only in wet years. In addition, only nitrogen decreased the stability of productivity. Thus, nutrient addition makes ecosystem productivity …

Characterization Of Inhibitory Molecules Produced By Lactobacillus Crispatus, Giuseppe Anthony Pistone

Master's Theses

Lactobacillus species are widely accepted as beneficial bacteria of the human microbiota1-8. Lactobacilli spp. are well documented to inhibit pathogens by production and secretion of hydrogen peroxide (H2O2), organic acids, and/or proteinaceous bacteriocins into their environment. Most research attributes bactericidal activity of cell free supernatant (CFS) to H2O2 and/or lactic acid. Here, I demonstrate that CFS from a clinical isolate of Lactobacillus crispatus contains a molecule(s) that inhibits uropathogenic Escherichia coli (UPEC) colony formation independently of H2O2 and organic acids. Physiologic concentrations of H2O2 and organic acids produced by L. crispatus do not inhibit UPEC colony formation. Incubation of UPEC …

Cycloaddition Reactions Of (Di)Tetrelenes, Nada Yahya Tashkandi

Electronic Thesis and Dissertation Repository

This thesis examines a variety of cycloaddition reactions of multiply-bonded heavy analogues of carbon. With the aid of a mechanistic probe, the mechanism of the addition of alkynes to a germene and a digermyne was examined. In both cases, the addition does not involve the itermediacy of an vinyl radicals or cations.

The addition of a variety of nitro compounds to tetramesityldisilene and tetramesityldigermene was examined. The facile formation of the novel 1,3,2,4,5-dioxazadisil- and digermolidine ring systems, respectively, was observed. In general, 1,3,2,4,5-dioxazadisilolidines and -digermolidines isomerize under thermal conditions to the 1,4,2,3,5-dioxazadisilolidines and -digermolidines ring system. The 1,3,2,4,5-dioxazadisilolidine and digermolidine …

Psychological Flexibility As A Mechanism Of Change In Acceptance And Commitment Therapy, Joseph Ciarrochi, Claire Godsell, Linda Bilich

joseph Ciarrochi

No abstract provided.

Embracing Multiple Definitions Of Learning, Andrew B. Barron, Eileen A. Hebets, Thomas A. Cleland, Courtney L. Fitzpatrick, Mark E. Hauber, Jeffrey R. Stevens

Eileen Hebets Publications

Definitions of learning vary widely across disciplines, driven largely by different approaches used to assess its occurrence. These definitions can be better reconciled with each other if each is recognized as coherent with a common conceptualization of learning, while appreciating the practical utility of different learning definitions in different contexts.

Learning is a major focus of research in psychology, neuro- science, behavioral ecology, evolutionary theory, and computer science, as well as in many other disciplines. Despite its conceptual prevalence, definitions of learning differ enormously both within and between these disciplines, and new definitions continue to be proposed [1]. Ongoing disputes …

Mutations In The Plasmodium Falciparum Chloroquine Resistance Transporter, Pfcrt, Enlarge The Parasite's Food Vacuole And Alter Drug Sensitivities, Serena Pulcini, Henry M. Staines, Andrew H. Lee, Sarah H. Shafik, Guillaume Bouyer, Catherine M. Moore, Daniel A. Daley, Matthew J. Hoke, Lindsey M. Altenhofen, Heather J. Painter, Jainbing Mu, David J.P. Ferguson, Manuel Llinás, Rowena E. Martin, David A. Fidock, Roland A. Cooper, Sanjeev Krishna

Biological Sciences Faculty Publications

Mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, are the major determinant of chloroquine resistance in this lethal human malaria parasite. Here, we describe P. falciparum lines subjected to selection by amantadine or blasticidin that carry PfCRT mutations (C101F or L272F), causing the development of enlarged food vacuoles. These parasites also have increased sensitivity to chloroquine and some other quinoline antimalarials, but exhibit no or minimal change in sensitivity to artemisinins, when compared with parental strains. A transgenic parasite line expressing the L272F variant of PfCRT confirmed this increased chloroquine sensitivity and enlarged food vacuole phenotype. Furthermore, the introduction …

An Activity Aimed At Improving Student Explanations Of Biological Mechanisms, Caleb M. Trujillo, Trevor R. Anderson, Nancy J. Pelaez

PIBERG Instructional Innovation Materials

This document is intended for use by instructors and their students. The activity contains steps to introduce students to the MACH model involving analyzing and discussing explanations about biological mechanisms. Initially, students read modified articles about biological mechanisms during class, although instructors may prefer to assign readings outside of class before the activity. During the activity, students are required to analyze the readings for evidence of research methods, analogies, context, and mechanisms. In so doing, students learn how to integrate the information pertaining to each of the MACH model components into a coherent explanation about their biological mechanism. After performing …