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Articles 1 - 17 of 17
Full-Text Articles in Life Sciences
Amyloid Fibrils Of Human Fgf-1 Induced By Different Detergents, Zeina Ismael Ibrahem Alraawi
Amyloid Fibrils Of Human Fgf-1 Induced By Different Detergents, Zeina Ismael Ibrahem Alraawi
Graduate Theses and Dissertations
Nature achieves molecular self-assembly through the ordered growth of nanoscale building blocks with high efficiency to fabricate macromolecular architectures. One example of self- assembly is peptides folding onto protein is one of the most astounding biological self-assembly processes. When proteins aggregate to form amyloid fibers, the secondary structure of the protein converts from its native state to a cross-beta-sheet. Fibroblast growth factors (FGFs) possess an essential role in neuronal survival during development. In addition, they are involved in neural stem cell (NSC) proliferation. Fibroblast growth factors (FGFs) are well known to be synthesized in the central nervous system (CNS) and …
Studying The Stability Of Collagen/Heparin Coatings To Be Used In Cell Therapy Applications, Gavin Mussino
Studying The Stability Of Collagen/Heparin Coatings To Be Used In Cell Therapy Applications, Gavin Mussino
Biological Sciences Undergraduate Honors Theses
This honors thesis aims to investigate the reusability and performance of cell coatings for cell therapy applications. Cell therapy, which involves the use of human cells to repair or replace damaged tissues, holds immense potential for medical advancements. However, ensuring the survival and functionality of transplanted cells remains a significant challenge. We focused on studying the effectiveness of coatings applied to cells for improved cell growth and viability. The research project involved the preparation of the coatings using a layer-by-layer method and the subsequent seeding of cells. The coated cells were then subjected to a series of experiments to assess …
Comparative Studies On Biochemical And Pharmacological Profiles Of Unfractionated Heparins & Their Depolymerized Derivatives From Bovine, Ovine And Porcine Origins, Ahmed Kouta
Dissertations
Heparin and heparin related drugs have remained the anticoagulant of choice for medical and surgical indications. These drugs are comprised of poly-component sulfated chains of varying length and biophysical structure with direct and indirect pharmacological effects. Heparins produce their therapeutic effects by multiple mechanisms involving both the cellular and humoral targets. These drugs represent natural products which are isolated from mammalian tissue rich in mast cells. Currently used heparin and LMWHs are obtained from porcine mucosal tissue. Other animal sources including bovine and ovine tissues also provide additional sources for the manufacturing of heparins. While there may be microchemical and …
Design Of Hfgf1 Variant(S) With Increased Stability And Enhanced Bioactivity, Shilpi P. Agrawal
Design Of Hfgf1 Variant(S) With Increased Stability And Enhanced Bioactivity, Shilpi P. Agrawal
Graduate Theses and Dissertations
Fibroblast growth factors (FGFs) are involved in various cellular processes such as cell growth,proliferation, differentiation, migration, angiogenesis, wound healing and embryonic development. Human acidic fibroblast growth factor (hFGF1) binds non-selectively to all the four FGF-receptors and is therefore considered as a powerful mitogen with broadest specificity. However, pharmacological applications of hFGF1 are restricted due to the low thermal stability of the growth factor. hFGF1 has low thermodynamic stability under physiological temperatures which leads to impairment of cellular signaling process thereby preventing its potential mitogenic properties. hFGF1 has a heparin binding pocket at the C-terminus which comprises of positively charges residues. …
Cloning, Expression, Purification And Characterization Of Heparin-Binding Pocket Of Recombinant Fgf1, Quratulayn Ashraf
Cloning, Expression, Purification And Characterization Of Heparin-Binding Pocket Of Recombinant Fgf1, Quratulayn Ashraf
Graduate Theses and Dissertations
Fibroblast growth factors are polypeptide members of the FGF family, which to date comprises of at least 22 members. They belong to a group of growth factors and are involved in a variety of cellular processes including wound healing, angiogenesis, differentiation and development (organogenesis). Amongst FGF members, human acidic FGF-1 and basic FGF-2 are the most characterized. FGF-1 and FGF-2 are known to share more than 80% sequence similarity and have an identical structural fold. However, their biological roles are quite different. FGFs bind to heparin and heparan sulfate ligands through their heparin-binding pockets. The interactions are primarily electrostatic in …
The Effects Of Heparin Concentration, Storage Time, And Temperature On The Values Of Hematological Parameters In Cyprinus Carpio, Malgorzata Witeska, Justyna Biardzka, Joanna Kniaz
The Effects Of Heparin Concentration, Storage Time, And Temperature On The Values Of Hematological Parameters In Cyprinus Carpio, Malgorzata Witeska, Justyna Biardzka, Joanna Kniaz
Turkish Journal of Veterinary & Animal Sciences
The results of hematological analyses may be affected by preanalytical factors such as sampling procedure, type and concentration of anticoagulant, or conditions and time of blood storage. In the present study the effects of various concentrations of heparin (0-200 IU/cm3) and different times (2, 24, or 48 h) and temperatures (4 or 22 °C) of blood storage on the values of hematological parameters in Cyprinus carpio were evaluated. Increase in heparin concentration resulted in a decrease in frequency of erythroblasts, a reduction of leukocyte and thrombocyte counts, and a decrease in oxidative metabolic activity of phagocytes. Storage for 2 h …
Engineered Human Acidic Fibroblast Growth Factor (Fgf1) With An Enhanced Thermal And Proteolytic Stability, Duaa Abdullah Almansaf
Engineered Human Acidic Fibroblast Growth Factor (Fgf1) With An Enhanced Thermal And Proteolytic Stability, Duaa Abdullah Almansaf
Graduate Theses and Dissertations
Fibroblast growth factor receptor (FGFR) is made up of three significant domains. The most important domain is the intracellular domain where the dimerization and autophosphorylation occur. Fibroblast growth factor (FGF) interacts with specific FGFR to regulate many cellular processes during the embryonic stage. Furthermore, FGF is significant for adults because FGF plays an important role in regulating cellular differentiation as well as wound healing. The cellular regulating processes are initiated through binding FGF to heparin followed by binding FGF/heparin to FGFR to form FGF/heparin/FGFR complex. Thus, FGFR is dimerized and autophosphorylated. The phosphorylation of FGFR triggers downstream signaling pathways, which …
Heparin-Peptide Interactions, Jacqueline Anastasia Morris
Heparin-Peptide Interactions, Jacqueline Anastasia Morris
Graduate Theses and Dissertations
Heparin is a polydispersed sulfated molecule that is part of the family called glycosaminoglycans found in the extracellular matrix and cell surfaces. This molecule is extremely important for the activation of proteins and protein-receptor interactions that are responsible for downstream cell signaling pathways. Heparin has been isolated from porcine intestine and used as an anticoagulant for the prevention of embolisms, heart thrombosis, and clotting during heart surgeries. This so-called miracle drug was in use until 2008, when isolated batches were found to be contaminated with other glycosaminoglycans similar to heparin. From 2008, there has been a dire need for a …
Protein Behavior Directed By Heparin Charge And Chain Length, Burcu Baykal Minsky
Protein Behavior Directed By Heparin Charge And Chain Length, Burcu Baykal Minsky
Doctoral Dissertations
Glycosaminoglycans (GAGs), highly charged biological polyelectrolytes, are of growing importance as biomaterials and pharmaceutical drugs due to their immense range of physiological functions. They bind to many proteins; however, the degree of structural selectivity in GAG-protein interactions is largely unknown .Our studies have focused on the importance of heparin (a model GAG) charge and chain length in protein binding in order to explore its potential applications in biofunctional tissue scaffold materials, as polysaccharide drugs in anticoagulation, and as inhibitory agents in protein aggregation. We used electrospray ionization mass spectrometry, capillary electrophoresis, size exclusion chromatography, dynamic/static light scattering and electrostatic protein …
Aspirin And Low-Molecular Weight Heparin Combination Therapy Effectively Prevents Recurrent Miscarriage In Hyperhomocysteinemic Women., Pratip Chakraborty, Sayani Banerjee, Piyali Saha, Shyam Sundar Nandi, Sunita Sharma, Sourendra K. Goswami, Baidyanath Chakravarty, Syed N. Kabir
Aspirin And Low-Molecular Weight Heparin Combination Therapy Effectively Prevents Recurrent Miscarriage In Hyperhomocysteinemic Women., Pratip Chakraborty, Sayani Banerjee, Piyali Saha, Shyam Sundar Nandi, Sunita Sharma, Sourendra K. Goswami, Baidyanath Chakravarty, Syed N. Kabir
Journal Articles: Cellular & Integrative Physiology
The management of recurrent pregnancy loss (RPL) still remains a great challenge, and women with polycystic ovarian syndrome (PCOS) are at a greater risk for spontaneous abortion. Treatment with low-molecular-weight heparin (LMWH) has become an accepted treatment option for women with RPL; however, the subgroup of women, who are likely to respond to LMWH, has not been precisely identified. The present study evaluated the efficacy of LMWH with reference to PCOS and associated metabolic phenotypes including hyperhomocysteinemia (HHcy), insulin resistance (IR) and obesity. This prospective observational study was conducted at Institute of Reproductive Medicine, Kolkata, India. A total of 967 …
Disodium Edta Used As Anticoagulant Causes Hemolysis In Common Carp Blood, Malgorzata Witeska, Wioleta Wargocka
Disodium Edta Used As Anticoagulant Causes Hemolysis In Common Carp Blood, Malgorzata Witeska, Wioleta Wargocka
Turkish Journal of Veterinary & Animal Sciences
Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples. Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis). The nuclei released from destroyed cells changed shape from oval to round, and underwent gradual swelling and vacuolation, followed by karyolysis. The degree of observed changes was similar at all Na2EDTA concentrations (0.1, 0.5, and 1.0 mg/mL); on the other hand, percentage of abnormal and destroyed cells showed considerable differences among blood sampled from various carp individuals. The obtained results revealed that Na2EDTA caused gradual damage to …
The Effects Of Anticoagulant Choice And Sample Processing Time On Hematologic Values Of Juvenile Whooping Cranes, Joan Maurer, Betsy Reichenberg, Cristin Kelley, Barry K. Hartup
The Effects Of Anticoagulant Choice And Sample Processing Time On Hematologic Values Of Juvenile Whooping Cranes, Joan Maurer, Betsy Reichenberg, Cristin Kelley, Barry K. Hartup
Proceedings of the North American Crane Workshop
Blood collected from juvenile whooping cranes (Grus americana) in 2007 and 2008 was divided and placed in either the anticoagulant lithium heparin (LiHep) or tri-potassium ethylenediaminetetraacetic acid (K3EDTA) for diagnostic hematology. Thin smears were prepared from the anticoagulated blood in the field with no delay and in the laboratory after a 4-6-hour delay, and then used to determine differential and total leukocyte counts. Manual heterophil and eosinophil counts were greater in LiHep-treated samples compared to K3EDTA samples (P < 0.05), but there was no difference in the total leukocyte concentration or differential leukocyte counts between anticoagulants based on blood smears prepared with no delay (n = 15). Marked differences were noted in relative heterophil (P < 0.05) and lymphocyte (P < 0.05) counts and total leukocyte (P < 0.05) concentrations of K3EDTA-treated samples processed after the delay (n = 7), suggesting a negative effect on lymphocyte integrity from the anticoagulant. Microscopically, lymphocytes were more intact and easily differentiated from thrombocytes in LiHep-treated samples than K3EDTA, but modest thrombocyte clumping in the LiHep samples was a concern. Either anticoagulant appears adequate for diagnostic hematology in juvenile whooping cranes based on this limited analysis, but blood smears should be prepared immediately under controlled conditions for best results.
The Ligand-Binding Profile Of Hare: Hyaluronan And Chondroitin Sulfates A, C, And D Bind To Overlapping Sites Distinct From The Sites For Heparin, Acetylated Low-Density Lipoprotein, Dermatan Sulfate, And Cs-E, Ed Harris, Paul H. Weigel
The Ligand-Binding Profile Of Hare: Hyaluronan And Chondroitin Sulfates A, C, And D Bind To Overlapping Sites Distinct From The Sites For Heparin, Acetylated Low-Density Lipoprotein, Dermatan Sulfate, And Cs-E, Ed Harris, Paul H. Weigel
Department of Biochemistry: Faculty Publications
Abstract The hyaluronic acid receptor for endocytosis (HARE)/ Stabilin-2 is the primary systemic scavenger receptor for hyaluronan (HA), the chondroitin sulfates (CS), dermatan sulfate (DS), and nonglycosaminoglycan (GAG) ligands such as acetylated low-density lipoprotein (AcLDL), pro-collagen propeptides, and advanced glycation end products. We recently discovered that HARE is also a systemic scavenger receptor for heparin (Hep) (Harris EN, Weigel JA, Weigel PH. 2008. The human hyaluronan receptor for endocytosis [HARE/Stabilin-2] is a systemic clearance receptor for heparin. J Biol Chem. 283:17341–17350). Our goal was to map the binding sites of eight different ligands within HARE. We used biotinylated GAGs and …
P159 Is A Proteolytically Processed, Surface Adhesin Of Mycoplasma Hyopneumoniae: Defined Domains Of P159 Bind Heparin And Promote Adherence To Eukaryote Cells., T. A. Burnett, K. Dinkla, M. Rohde, G. S. Chhatwal, C. Uphoff, M. Srivasta, S. J. Cordwell, S. Geary, X. Liao, F. C. Minion, Mark J. Walker, S. P. Djordjevic
P159 Is A Proteolytically Processed, Surface Adhesin Of Mycoplasma Hyopneumoniae: Defined Domains Of P159 Bind Heparin And Promote Adherence To Eukaryote Cells., T. A. Burnett, K. Dinkla, M. Rohde, G. S. Chhatwal, C. Uphoff, M. Srivasta, S. J. Cordwell, S. Geary, X. Liao, F. C. Minion, Mark J. Walker, S. P. Djordjevic
Faculty of Science - Papers (Archive)
Mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, colonises the respiratory cilia of affected swine causing significant economic losses to swine production worldwide. Heparin is known to inhibit adherence of M. hyopneumoniae to porcine epithelial cilia. M. hyopneumoniae cells bind heparin but the identity of the heparin-binding proteins is limited. Proteomic analysis of M. hyopneumoniae lysates identified 27 kDa (P27), 110 kDa (P110) and 52 kDa (P52) proteins representing different regions of a 159 kDa (P159) protein derived from mhp494. These cleavage fragments were surface located and present at all growth stages. Following purification of 4 recombinant proteins spanning …
Heparin Modulates The 99-Loop Of Factor Ixa: Effects On Reactivity With Isolated Kunitz-Type Inhibitor Domains, Pierre F. Neuenschwander, Stephen R. Williamson, Armen Nalian, Kimberly J. Baker-Deadmond
Heparin Modulates The 99-Loop Of Factor Ixa: Effects On Reactivity With Isolated Kunitz-Type Inhibitor Domains, Pierre F. Neuenschwander, Stephen R. Williamson, Armen Nalian, Kimberly J. Baker-Deadmond
Faculty Publications
Reactivity of factor IXa with basic pancreatic trypsin inhibitor is enhanced by low molecular weight heparin (enoxaparin). Previous studies by us have suggested that this effect involves allosteric modulation of factor IXa. We examined the reactivity of factor IXa with several isolated Kunitz-type inhibitor domains: basic pancreatic trypsin inhibitor, the Kunitz inhibitor domain of protease Nexin-2, and the first two inhibitor domains of tissue factor pathway inhibitor. We find that enhancement of factor IXa reactivity by enoxaparin is greatest for basic pancreatic trypsin inhibitor (>10-fold), followed by the second tissue factor pathway inhibitor domain (1.7-fold) and the Kunitz inhibitor …
Two Domains Within The Mycoplasma Hyopneumoniae Cilium Adhesin Bind Heparin, C. Jenkins, J. L. Wilton, F. C. Minion, L. Falconer, Mark J. Walker, S. P. Djordjevic
Two Domains Within The Mycoplasma Hyopneumoniae Cilium Adhesin Bind Heparin, C. Jenkins, J. L. Wilton, F. C. Minion, L. Falconer, Mark J. Walker, S. P. Djordjevic
Faculty of Science - Papers (Archive)
Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia, a chronic and economically significant respiratory disease that affects swine production worldwide. M. hyopneumoniae adheres to, and adversely affects the function of ciliated epithelial cells of the respiratory tract, and the cilium adhesin (P97), is intricately but not exclusively involved in this process. Although binding of pathogenic bacteria to glycosaminoglycans is a recognised step in pathogenesis, knowledge of glycosaminoglycan-binding proteins is lacking in M. hyopneumoniae. However, heparin and other sulfated polysaccharides are known to block the binding of M. hyopneumoniae to purified swine respiratory cilia. In this study, four regions …
The Effect Of Heparin On Growth Characteristics Of Transplanted Spontaneously Occurring C3h/Hej Mouse Mammary Tumor, Muhammed Saleh Abdullah Banaja
The Effect Of Heparin On Growth Characteristics Of Transplanted Spontaneously Occurring C3h/Hej Mouse Mammary Tumor, Muhammed Saleh Abdullah Banaja
University of the Pacific Theses and Dissertations
Mouse mammary tumor is a common form of cancer used for experimental purposes. It is relatively resistant to chemotherapy. This investigation is designed to test the effect of heparin, a naturally occurring anticoagulant, on tumor growth using a genetically uniform laboratory animal.