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Genetics And Biochemistry Of Protein Glycosylation In Campylobacter Jejuni, Alexandra Merkx-Jacques, S. Vijayakumar, M. Demendi, D. Ratnayake, C. Dozois, C. Creuzenet
Genetics And Biochemistry Of Protein Glycosylation In Campylobacter Jejuni, Alexandra Merkx-Jacques, S. Vijayakumar, M. Demendi, D. Ratnayake, C. Dozois, C. Creuzenet
Alexandra Merkx-Jacques
BACKGROUND: C. jejuni produces numerous glycoproteins, including flagellins, which are important for virulence. The flagellins harbour pseudaminic acid (PA) whereas other glycoproteins harbour diacetamidobacillosamine (DAB). We are investigating the genetics and biochemistry of protein glycosylation in C. jejuni to identify the enzymes involved, and determine their activity and roles in virulence. We focused on two homologous pathways comprising each a putative dehydratase, aminotransferase and acetyltransferase, namely {Cj1293, Cj1294 and Cj1298} and {Cj1120c, Cj1121c and Cj1123c}.
METHODS: All enzymes were overexpressed and purified before monitoring their activity by capillary electrophoresis. The cj1121c and cj1294 genes were disrupted by a chloramphenicol resistance …