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Full-Text Articles in Life Sciences
Microscale Patchiness Of Bacterioplankton Assemblage Richness In Seawater, Richard A. Long, Farooq Azam
Microscale Patchiness Of Bacterioplankton Assemblage Richness In Seawater, Richard A. Long, Farooq Azam
Faculty Publications
We sought to test the hypothesis that bacterial species richness and composition vary at the millimeter scale in the marine pelagic environment, in response to the heterogeneous distribution of organic matter. To test this hypothesis, it was necessary to design and test a protocol to sample, lyse and analyze (by polymerase chain reaction/denaturing gradient gel electrophoresis [PCR-DGGE]) bacterioplankton species richness in a single microliter of seawater. Significant variation in bacterial species richness was found amongst 1 μl samples. Greater species richness was seen when seawater was enriched with diatom detritus. Examination of species richness at 2 mm intervals over a …
Variations In Bacterial Community Structure During A Dinoflagellate Bloom Analyzed By Dgge And 16s Rdna Sequencing, Laura B. Fandino, Lasse Riemann, Grieg F. Steward, Richard A. Long, Farooq Azam
Variations In Bacterial Community Structure During A Dinoflagellate Bloom Analyzed By Dgge And 16s Rdna Sequencing, Laura B. Fandino, Lasse Riemann, Grieg F. Steward, Richard A. Long, Farooq Azam
Faculty Publications
The relationship between bacterial 16S rRNA gene composition and carbon metabolism was analyzed during an intense dinoflagellate bloom off the Southern California coast during the spring of 1997. Bacterial numbers and rate processes, chlorophyll a, and the dissolved and particulate organic matter pools were measured during the bloom to provide a framework within which to assess bacterial community composition. Free bacteria were numerically dominant, generally comprising >90% of the total, and were responsible for >70% of bacterial production. Attached bacteria had higher cell-specific growth rates than free bacteria (range = 0.5 to 15.1 and 0.7 to 2.5 d-1 …
Nitric Oxide And Cnidarian-Dinoflagellate Symbioses: Pieces Of A Puzzle, Henry G. Trapido-Rosenthal, Katherine H. Sharp
Nitric Oxide And Cnidarian-Dinoflagellate Symbioses: Pieces Of A Puzzle, Henry G. Trapido-Rosenthal, Katherine H. Sharp
Arts & Sciences Faculty Publications
The presence of nitric oxide synthase (NOS) activity is demonstrated in the tropical marine cnidarian Aiptasia pallida and in its symbiotic dinoflagellate algae, Symbiodinium bermudense. Enzyme activity was assayed by measuring the conversion of arginine to citrulline. Biochemical characterization of NOS from Aiptasia was characterized with respect to cellular localization, substrate and cofactor requirements, inhibitors, and kinetics. In response to acute temperature shock, anemones retracted their tentacles. Animals subjected to such stress had lower NOS activities than did controls. Treatment with NOS inhibitors caused tentacular retraction, while treatment with the NOS substrate L-arginine inhibited this response to stress, as did …
Taxonomy And Biology Of A New Oecophoridae (Lepidoptera) From Central Chile, T. Heath Ogden
Taxonomy And Biology Of A New Oecophoridae (Lepidoptera) From Central Chile, T. Heath Ogden
T. Heath Ogden
The adult, larva, and pupa of Afdera jimenae Ogden & Parra sp. nov. are described and illustrated. Larvae live in leaf litter throughout all instars and are generalists feeding upon the fallen leaves of a number of different plant species of sclerophyllous forests. Comments on morphological details and bionomics of this species are given. This is the second species of Afdera know for Chile.