Life Sciences Commons^™

Use Of Biomass From Reed Canary Grass (Phalaris Arundinacea) As Raw Material For Production Of Paper Pulp And Fuel, B Andersson, E Lindvall

IGC Proceedings (1997-2023)

Reed canary grass (Phalaris arundinacea) has been investigated as a potential industrial crop in Sweden and other European countries. Reed canary grass (RCG) can be used as raw-material for paper pulp or as biofuel for combustion. A new harvest method, the delayed harvesting system, was developed which makes it possible to get dry, ready to store, material. The method reduces cost and improves quality of the product. The use of RCG as an industrial crop requires a completely different quality compared to forage. The important part is the cellulose while protein and mineral nutrients are disturbing the process …

Breeding Reed Canarygrass As An Energy Or Fibre Crop By Using Local Collected Wild Populations, E Lindvall

IGC Proceedings (1997-2023)

Reed canarygrass (Phalaris arundinacea L.) has become interesting as an industrial crop in Sweden. The grass can be used as biofuel or fibre raw material for pulp production. New varieties have to be developed, with a quality different from forage varieties. High stem/ leaf-ratio, low content of ash and elements like silica, potassium and chlorine are important breeding goals. A Swedish project, aiming to evaluate local reed canarygrass populations, shows that there is some variation in these morphological and chemical characters which can be utilised in a breeding program.

Exploring A Medicinal Strain Of White-Rot Fungus For Biofuel Applications, Kortney L. Lucius

Masters Theses

The major limitation to producing biofuels from lignocellulosic biomass, such as miscanthus straw, is the difficulty in accessing fermentable carbohydrates encased in lignin. Traditional methods for separating lignin are economically and environmentally costly. Biological pretreatment using white-rot fungi, such as Trametes versicolor, has been demonstrated to potentially reduce the need for harsh chemical and physical pretreatment methods. Despite these efforts, the process of converting lignocellulose to ethanol remains financially challenging. To explore a potential co-product for the process, this study evaluated the efficacy of T. versicolor strain CM-101, known for producing the valuable medicinal macromolecule polysaccharide-K (PSK), as a …

Synthesis Of Bacterial Glycerophospholipids For Biomembrane Model Studies: A Means To Advanced Biofuels, Felix Adulley

Electronic Theses and Dissertations

To reduce reliance on fossil fuels, sustainable biofuels are being pursued, especially advanced biofuels like 1-butanol that have higher energy content and greater compatibility with existing infrastructure than ethanol. A persistent challenge is the yield-limiting toxicity of biofuels and process solvents, such as tetrahydrofuran, to the microbes that ferment biomass into biofuel. The cell membrane is a focal point of toxicity, and understanding how it interacts with fuels and solvents is key to improving yield. Phospholipid bilayers are the core of biomembranes, and model biomembranes of defined composition provide the ideal platform for biophysical studies. To this end, glycerophospholipids characteristic …

Grass Pellet Bioenergy In The Northeastern Usa, J. H. Cherney, D. J. R. Cherney

IGC Proceedings (1997-2023)

Grass pellets are a renewable energy supply that combines low technology/small-scale with local production/consumption for a cost effective energy system. There have been significant recent advances in pellet furnace technology and some pellet stove manufacturers now claim their stoves are capable of burning biomass with 5-6% ash content. Cool-season grasses have not been considered acceptable for pelleting and direct combustion in the past due to high ash content. Rain after harvest, however, has been shown to leach significant amounts of potassium and chlorine from grass (Sander, 1997). High yields are possible under lax harvest management (Cherney et al., 2003), producing …

A Review On Potential Biofuel Yields From Cover Crops, Liangcheng Yang, Lucas D. Lamont, Shan Liu, Chunchun Guo, Shelby Stoner

Faculty Publications - Agriculture

Millions of hectares of cover crops are planted in the U.S. and European Union to manage soil erosion, soil fertility, water quality, weeds, and climate change. Although only a small percentage of cover crops are harvested, the growing cover crop planting area provides a new biomass source to the biofuel industry to produce bioenergy. Oilseed crops such as rapeseed, sunflower, and soybean are commodities and have been used to produce biodiesel and sustainable aviation fuel (SAF). Other cover crops such as cereal rye, clover, and alfalfa, have been tested on small or pilot scales to produce cellulosic ethanol, biogas, syngas, …

Morphological, Physiological, And Transcriptional Responses Of Populus Trichocarpa To Water Deficit Stress, Kristin Justine Ratliff

Graduate Theses, Dissertations, and Problem Reports

Under the threat of climate change with continued fossil fuel use, investment in developing renewable energies is increasingly important. One category of renewable energy sources, biofuels, is well suited to industries that require a liquid fuel source. However, producing biofuel feedstocks is challenging from a sustainability perspective, as they require extensive land area and resource input to grow on marginal lands. The identification of strategies that feedstock species use to maintain productivity with limited to no resource inputs could aid in the selection of optimal genotypes or targets of breeding or engineering efforts to maximize productivity in feedstock plantations. Some …

Insights Into The Biotechnology Potential Of Methanosarcina, Sean Carr, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Methanogens are anaerobic archaea which conserve energy by producing methane. Found in nearly every anaerobic environment on earth, methanogens serve important roles in ecology as key organisms of the global carbon cycle, and in industry as a source of renewable biofuels. Environmentally, methanogenic archaea play an essential role in the reintroducing unavailable carbon to the carbon cycle by anaerobically converting low-energy, terminal metabolic degradation products such as one and two-carbon molecules into methane which then returns to the aerobic portion of the carbon cycle. In industry, methanogens are commonly used as an inexpensive source of renewable biofuels as well as …

Nutrient Recovery From Wastewater By A Consortium Of Algae Species For Biofuel Production, Edgardo Ayala

Boise State University Theses and Dissertations

Current energy sources are predominantly petroleum-based and their use increases greenhouse gas (GHG) emissions. As the global population grows, and along with it the demand for energy, there is a need to further develop renewable energy sources to avoid the effects of increasing atmospheric CO₂ concentrations on the climate. Biofuels, a renewable energy source, have gained significant interest as a replacement for petroleum-based fuels due to their environmental benefits and carbon neutrality. Biofuels are expected to make up 9.0% of the total fuel consumption in the U.S. by 2040, up from 7.3% in 2019 [1]. Currently, terrestrial crop-based biofuels …

Sorption Of Cellulases In Biofilm Enhances Cellulose Degradation By Bacillus Subtilis, Yijie Deng, Shiao Y. Wang

Faculty Publications

Biofilm commonly forms on the surfaces of cellulosic biomass but its roles in cellulose degradation remain largely unexplored. We used Bacillus subtilis to study possible mechanisms and the contributions of two major biofilm components, extracellular polysaccharides (EPS) and TasA protein, to submerged biofilm formation on cellulose and its degradation. We found that biofilm produced by B. subtilis is able to absorb exogenous cellulase added to the culture medium and also retain self-produced cellulase within the biofilm matrix. The bacteria that produced more biofilm degraded more cellulose compared to strains that produced less biofilm. Knockout strains that lacked both EPS and …

The Effects Of Genotype And Nutrient Conditions On Poplar Growth And Physiology, Bethanie Natalie

Theses - ALL

The United States is currently reliant on other nations for energy and has been unable tomeet goals set for domestic production of 'green' energy such as second generation biofuel. Overcoming these challenges requires examination of transgenic biofuel feedstock plants, specifically in terms of their responses to two nutrient treatments. Here, we investigated the photosynthetic and growth impacts of expressing a hyperthermophilic endoglucanase in planta in a wildtype (WT) Populus alba × grandidentata background under 100% and 15% strength nutrient treatments. This gene was inserted for improvement of the pretreatment process and to limit inputs required during biofuel production. We characterized …

The Influences Of Promising Feedstock Variability On Advanced Biofuel Production: A Review, Cherng-Yuan Lin

Journal of Marine Science and Technology

The contribution of biofuels is expected to continuously increase in the global fuel market, as they are environmentally-friendly and provide renewable energy. Four generations of biofuels are categorized and are primarily based on their feedstock sources and the production technologies that are used. The influence of promising feedstock types and the availability on the production technologies and the fuel properties of advanced-generation biofuels are not systematically examined in the literature. Hence, this research extensively reviews the potential impact of feedstock sources and their variability on the production and characteristics of biofuels. The approaches of theoretical analysis and inference referred to …

Toward Lignin Valorization: Development Of Rhodococcus Opacus Pd630 As A Chassis For Triacylglycerol (Tag) Production From Recalcitrant Aromatic Feedstocks, Rhiannon R. Carr

McKelvey School of Engineering Theses & Dissertations

The advent of the industrial era was precipitated by the discovery of fossil fuels, and ushered in unprecedented changes for humanity included but not limited to the development of rapid transit and communications, improvements to food distribution and preservation, the mass production of goods, and a radical rearrangement of communities from relatively small enclaves to metropolises. With all the benefits, however, come considerable costs, especially to the global environment. Greenhouse gas emissions, built up over centuries of unregulated combustion, have precipitated a rate of global temperature change unparalleled in the 4.5 billion-year history of this planet. In order to preserve …

A Sugarcane G-Protein-Coupled Receptor, Shgpcr1, Confers Tolerance To Multiple Abiotic Stresses, Manikandan Ramasamy, Mona B. Damaj, Carol Vargas-Bautista, Victoria Mora, Jiaxing Liu, Carmen S. Padilla, Sonia Irigoyen, Tripti Saini, Nirakar Sahoo, Jorge A. Dasilva, Kranthi K. Mandadi

Biology Faculty Publications and Presentations

Sugarcane (Saccharum spp.) is a prominent source of sugar and serves as bioenergy/biomass feedstock globally. Multiple biotic and abiotic stresses, including drought, salinity, and cold, adversely affect sugarcane yield. G-protein-coupled receptors (GPCRs) are components of G-protein-mediated signaling affecting plant growth, development, and stress responses. Here, we identified a GPCR-like protein (ShGPCR1) from sugarcane and energy cane (Saccharum spp. hybrids) and characterized its function in conferring tolerance to multiple abiotic stresses. ShGPCR1 protein sequence contained nine predicted transmembrane (TM) domains connected by four extracellular and four intracellular loops, which could interact with various ligands and heterotrimeric G …

Annotated Genome Sequence Of The High-Biomass-Producing Yellow-Green Alga Tribonema Minus, Kristina M. Mahan, Jüergen E.W. Polle, Zaid Mckie-Krisberg, Anna Lipzen, Alan Kuo, Igor V. Grigoriev, Todd W. Lane, Aubrey K. Davis

Publications and Research

Here, we report the annotated genome sequence for a heterokont alga from the class Xanthophyceae. This high-biomass-producing strain, Tribonema minus UTEX B 3156, was isolated from a wastewater treatment plant in California. It is stable in outdoor raceway ponds and is a promising industrial feedstock for biofuels and bioproducts.

Plant Diversity And Nitrogen Addition On Belowground Biodiversity And Soil Organic Carbon Storage In Biofuel Cropping Systems, Jennifer Butt

Boise State University Theses and Dissertations

Bioenergy production may reduce the emission of CO₂ which contributes to climate change, particularly when management strategies are adopted that promote soil carbon (C) sequestration in bioenergy cropping systems. Planting perennial native grasses, such as switchgrass (Panicum virgatum L.) and big bluestem (Andropogon gerardii Vitman) may be used as a strategy to enhance soil C accumulation owing to their extensive root systems. Fertilizer use may further promote soil C sequestration, because of its positive impacts on plant production and soil C input. However,, the influence of fertilizer addition on soil C accumulation is variable across bioenergy cropping …

Lipid Analysis, Fluorescence Microscopy And Flow Cytometry As Tools To Evaluate Microalgae For Potential Biofuel Production, Giovanina Aimee Hinojosa

Theses and Dissertations

Petroleum-based crude oil is nonrenewable and toxic to the environment, leading to a need for a more efficient and environmentally effective replacement. Microalgae produce lipids which can be converted to recyclable biofuels; therefore, research in lipid production by algae is becoming important for an economic and environmental sustainability. Microalgae can re-absorb atmospheric CO2 and have rapid growth, thereby lowering greenhouse gasses (GHGs) emission, renewability, and simple growth requirements.

Nannochloris sp. is a marine microalga that is amenable for lipid production because there is little research on it so this study will focus on using flow cytometry, fluorescence microscopy and other …

Novel Approaches To Developing On-Farm Biomass Production Systems, S. Ray Smith, Thomas Clarkson Keene, Lee Carol Greenwell, Krista L. Cotten

IGC Proceedings (1997-2023)

Energy security and climate change are issues facing many countries today. Finding clean, renewable fuel sources has provided a challenge to look beyond the obvious and search for new ways to use old resources. Switchgrass (Panicum virgatum L.) is a warm season grass native to many parts of the United States. In 2007, the University of Kentucky (UK) College of Agriculture started a collaborative project with the UK Cooperative Extension, farmers in northern Kentucky, Kentucky Forage and Grassland Council and East Kentucky Power Cooperative to explore and demonstrate how switchgrass can be grown, harvested and utilized in an economical …

Growing Biofuel Feedstocks In Copper-Contaminated Soils Of A Former Superfund Site, Virinder Sidhu, Dibyendu Sarkar, Rupali Datta

Michigan Tech Publications

Copper mining in the Upper Peninsula of Michigan in the mid-19th century generated millions of tons of mining waste, called stamp sand, which was deposited into various offshoots of Lake Superior. The toxic stamp sand converted the area into barren, fallow land. Without a vegetative cover, stamp sand has been eroding into the lakes, adversely affecting aquatic life. Our objective was to perform a greenhouse study, to grow cold-tolerant oilseed crops camelina (Camelina sativa) and field pennycress (Thlaspi arvense) on stamp sand, for the dual purpose of biofuel production and providing a vegetative cover, thereby decreasing erosion. Camelina and field …

The Role Of Endophytes In Conferring Salinity Tolerance In Prairie Cordgrass And The Winter Wheat Mint Variety, Jeffery Bartel

Electronic Theses and Dissertations

Prairie Cordgrass (PCG) Spartina pectinata is a native grass to North America that survives saline conditions, can be harvested multiple times in a year, and can be used as biofuel. Endophytes are bacteria that reside in plant tissues and often have growthpromoting capabilities. They can assist plants by obtaining nutrients by improving the tolerance of plants against a broad range of stresses, such as plant pathogens, viruses, flooding, drought, acids, or bases. PCG can survive ocean levels of salinity. Endophytes that naturally bond with PCG may not only be able to not only help PCG but be transferred to other …

Soil Sustainability Of Forest Bioenergy Feedstocks Across The Americas, Michelle Brill

Dissertations, Master's Theses and Master's Reports

Many sources of woody biofuels provide alternative options to fossil fuels that can help mitigate greenhouse gases. One key component of feedstock sustainability from an ecosystem service perspective is soil sustainability. We examined eucalyptus (Eucalyptus grandis) plantations in the warm-temperate entic and mollic soils of Northeastern Argentina, oil palm plantations (Elaeis guineensis Jacq.) in the tropical alfic soils of Southeastern Mexico, and aspen (Populus tremuloides) in the cool-temperate spodic soils of Northeastern United States. The following elements were measured in soil increments of 15 cm, down to a total of 60 cm: carbon (C), nitrogen …

Glycolic Acid Utilization In Two Species Of Marine Bacteria, Erik S. Timsak

STAR Program Research Presentations

Interactions between bacteria and phytoplankton are crucial for the cycling of organic matter in marine environments. Around 50% of organic carbon taken up by marine bacteria is converted into inorganic carbon. The uptake of organic carbon by marine bacteria exuded from phytoplankton is a key factor in regulating the marine carbon cycle. One such molecule that is exuded by phytoplankton and then uptaken by marine bacteria is called glycolate - the anion of glycolic acid, a two caron molecule. Glycolate is exuded by phytoplankton during photorespiration and 10-50% of dissolved organic carbon in marine environments is comprised of glycolate. Additionally, …

Rust And Viral Mosaic Diseases In Biofuel Switchgrass, Anthony A. Muhle

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Switchgrass (Panicum virgatum L.) is a perennial warm-season monocot that is indigenous to locations in North America east of the Rocky Mountains, and is considered a model grass for biofuel feedstock production. As switchgrass production increases, diseases pose a potential threat to biomass production and ethanol extraction. The two predominant switchgrass diseases in Nebraska are rust caused by Puccinia spp. and a viral mosaic disease caused by Panicum mosaic virus (PMV) and its associated Satellite panicum mosaic virus (SPMV). In this thesis, one study determined how SPMV affects PMV infection and systemic spread in two populations of switchgrass at different …

Descriptions Of Life-Stages Of Blastobasis Repartella (Lepidoptera: Gelechioidea: Coleophoridae: Blastobasinae) And Observations On Its Biology In Switchgrass, D. Adamski, P. J. Johnson, A. A. Boe, Jeffrey Bradshaw, Alan Pultyniewicz

Arvid Boe

Blastobasis repartella (Dietz) is a borer in the proaxis and basal nodes and internodes of above ground stems of Panicum virgatum L. (Poaceae). The adult and immature stages are described herein, including diagnoses of the adult and larva, as this insect may be easily confused with a closely related grass-feeding congener, Blastobasis graminea Adamski, which is also known to occur in the United States. The biology of B. repartella is described. Figures of the adult, illustrations of the male and female genitalia, wing venation, the chaetotaxy of the larva (supplemented with scanning electron micrographs), and pupa are provided. Bassus difficilis …

Symptoms, Distribution And Abundance Of The Stem-Boring Caterpillar, Blastobasis Repartella (Dietz), In Switchgrass, Jarrad R. Prasifka, Jeffrey Bradshaw, Arvid A. Boe, Dokyoung Lee, David Adamski, Michael E. Gray

Arvid Boe

A potential pest of switchgrass, Panicum virgatum L., was first detected in South Dakota in 2004, where death of partially emerged leaves was noted in a small proportion of tillers. Similar “dead heart” symptoms were observed in switchgrass in Illinois during 2008 and adults of a stem-boring caterpillar were collected and identified as Blastobasis repartella (Dietz). In 2009, a survey of the central United States was used to estimate the distribution and abundance of this insect. In eight northern states, B. repartella was consistently found in both cultivated plots and natural stands of switchgrass. In four southern states, B. repartella …

Field Evaluation Of Tobacco Engineered For High Leaf-Oil Accumulation, James Perry

Theses and Dissertations--Plant and Soil Sciences

The biofuel market is dominated by ethanol and biodiesel derived from cellulosic and lipid-based biomass crops. This is largely due to the relatively low costs and reliability of production. At present, production of non-food plant-derived oils for biofuel production in the U.S. is minimal. A research team from the Commonwealth Scientific and Industrial Research Organization (CSIRO), an independent Australian federal government research institution, has developed an efficient transgenic system to engineer oil production in tobacco leaves. This novel system is comprised of multiple transgenes that direct the endogenous metabolic flux of oil precursors towards triacylglycerol (TAG) production. Additional genes were …

Fractionation And Characterization Of Lignin Streams From Unique High-Lignin Content Endocarp Feedstocks, Wenqi Li, Kirtley Amos, Mi Li, Yunqiao Pu, Seth Debolt, Arthur J. Ragauskas, Jian Shi

Biosystems and Agricultural Engineering Faculty Publications

Background: Lignin is a promising source of building blocks for upgrading to valuable aromatic chemicals and materials. Endocarp biomass represents a non-edible crop residue in an existing agricultural setting which cannot be used as animal feed nor soil amendment. With significantly higher lignin content and bulk energy density, endo-carps have significant advantages to be converted into both biofuel and bioproducts as compared to other biomass resources. Deep eutectic solvent (DES) is highly effective in fractionating lignin from a variety of biomass feedstocks with high yield and purity while at lower cost comparing to certain ionic liquids.

Results: In the present …

Reducing Cell Wall Recalcitrance In Poplar Through Overexpression Of Cell Wall Degrading Enzymes, Yao Xiao

Dissertations - ALL

Abundant cellulose in the secondary cell walls of lignocellulosic biomass provides an alternative source of carbohydrates for ethanol production. However, low efficiency and high cost of conversion of cellulose to glucose prevents the large-scale production of economically viable cellulosic ethanol. Expression of glycosyl hydrolases in plants has been proposed as an ideal replacement for current microbial-cellulase production in order to reduce the investment cost of hydrolases for ethanol production.

In this thesis, a hyperthermophilic endoglucanase, TnCelB, was expressed in hybrid poplar. The transgenic event with highest TnCelB expression level had reduced lignin content, cellulose crystallinity, and resultant more digestible cell …

Creating Renewable Energy Through The Pairing Of Wastewater Remediation And Biofuel Production With Two Species Of Microalgae, Laura Brown

Master's Theses

In this study, two species of microalgae, Scenedesmus sp. isolated locally and Chlorella sorokiniana (UTEX 1230) were grown in municipal wastewater collected from Stennis Space Center, MS in order to assess whether or not the microalgae could remediate the wastewater into a clean and usable form while also producing the biomaterials necessary for fuel and other useful byproducts. Each species was cultured in 16 ˚C and 30 ˚C to assess functional variabilities at different times of the year. The microalgae’s ability to decrease nutrient concentrations in the wastewater and produce lipids and protein was assessed at three different growth stages: …

Nannochloris Sp. And Oocystis Sp. Algae Exposed To Osmotic Stress For Increased Lipid Production For Use In Biofuels, Laura M. Chavez

Theses and Dissertations

Algal biofuel research is important due to the potential need for replacement of non-renewable fossil fuels and a changing global climate. Under stress, algal cells tend to increase neutral lipid production, which are a suitable source for biofuels. The goal of this study was to subject Nannochloris sp.. and Oocystis sp. algal cells to osmotic stress by modifying sucrose and salinity levels in different treatment medias.

The treatment containing the lowest salinity (15 ppt) and no sucrose yielded the highest lipid fluorescence/cell in Nannochloris sp.. cells (0.00142 ± 0.00044) on day 2 while the treatment containing highest salinity (35ppt) …