Life Sciences Commons^™

The Detectability Of Atlantic Salmon (Salmo Salar) Microsatellite And Mitochondrial Environmental Dna, Simone N. Miklosi

Electronic Thesis and Dissertation Repository

Little is known about what information can be gathered from microsatellite eDNA. It is important to gain a deeper understanding of the detectability and analysis of microsatellite eDNA because it could provide information about population size that mitochondrial eDNA cannot. Water samples were collected from tank and river experiments, and rivers known to contain Atlantic salmon (Salmo salar) and analyzed for Atlantic salmon mitochondrial and microsatellite eDNA. Mitochondrial eDNA was detected from all the tank experiments and 10 out of 15 rivers known to contain Atlantic salmon. Microsatellite eDNA was detected from all the tank experiments and none …

Biophysical Factors Impacting Sea Lice Settlement And Survival, Eleanor R. Glahn

Electronic Theses and Dissertations

Atlantic salmon (Salmo salar L.) aquaculture production in Maine is a valuable contributor to the economy, the expansion of which has been challenged by the parasitic salmon louse Lepeophtheirus salmonis. As planktonic organisms, the life of the salmon louse is primarily dictated by the physical conditions of the environment: the temperature for development time, salinity for survival, and current velocity for transport. Salmon lice are obligate parasites for whom the successful infection of a suitable host is critical to completion of their life cycle. However, little is understood about the effects of current velocity on infection success. Hydrodynamic …

Distribution And Relative Abundance Of Reintroduced Atlantic Salmon, Salmo Salar, In Lake Ontario Tributaries Using Environmental Dna, Nabeelah Lulat

Electronic Theses and Dissertations

Plans to reintroduce Atlantic salmon in Lake Ontario tributaries consists of stocking hatchery-reared fish yearly which will help to achieve a self-sustaining population. The issue with reintroduction remains in understanding the distribution of fishes after stocking. Environmental DNA (eDNA) provides a sensitive approach for monitoring that can offer inferences into fish distribution. I determined the distribution of stocked Atlantic salmon downstream from stocking sites using qRT-PCR and metabarcoding. I found that Atlantic salmon eDNA detection was more sensitive using qRT-PCR (51%) versus metabarcoding HTS (18.3%). However, metabarcoding provided data on fish community assemblages, which can help to monitor ecological interactions. …

The Effect Of Freezing Rates And Storage On Atlantic Salmon And Peaches, Wesam Aljeddawi

All Dissertations

The effect of long-term frozen storage of Atlantic salmon and sliced peaches was studied to determine methods to optimize quality. The first experiment was conducted to determine the quality changes in Atlantic salmon stored at different freezer temperatures over 12 months. Fresh and pre-frozen salmon were placed in five different freezers set at –7°C, –12°C, –18°C, –29°C and –77°C and evaluated for quality at 30, 90, 180, 270 and 360 days of storage. In general, higher quality was retained to a greater extent in salmon held at –29°C and –77°C for 360 days compared to the other storage temperatures. No …

The Long-Term Impact Of Dam Removals On Penobscot River Migratory Fishes, Erin Peterson

Electronic Theses and Dissertations

Dams interrupt river connectivity and disrupt fish migrations. We used telemetry to study the migratory movement patterns of adult American shad, sea lamprey, and Atlantic salmon on the Penobscot River, Maine after dam removals and other passage improvements had occurred. We also studied scale formation of marine-stage Atlantic salmon raised in marine net pens, the findings from which could be relevant to captive rearing efforts.

American shad now have access to the majority of their historic spawning habitat, contingent on passage the first main-stem dam (Milford Dam). We found that habitat upstream of dams was infrequently accessed, and first …

The Effect Of Large Wood Restoration On Algal Biomass And Spatial Distribution In The Narraguagus River, Kiera M. Luu

Honors College

The Atlantic Salmon (Salmo salar) is an anadromous fish native to the eastern US and Canada. Though they used to inhabit much of the eastern coasts of New England and Canada, the last of the United States’ wild Atlantic Salmon are now mostly limited to Maine. Due to habitat destruction from dams and historic logging activity, the quality of food sources, spawning grounds, and essential juvenile salmon habitat have been severely impacted. This habitat is the rearing area for many young salmon, and its quality influences their growth and recruitment into the overall Atlantic salmon population. Restoration of Atlantic salmon …

How To Avoid The Use Of Chemicals To Control Parasites In Salmon Farming?, Cintya Gajardo Vejar

English Language Institute

The development of salmon farming in the world has been accompanied by a number of conditions like parasitism by Sea Lice (Caligus rogercresseyi), this problem occurs in the fattening stage of Atlantic Salmon (Salmo salar) in the sea, increasing mortality and generating economic losses. The salmon industry efforts have focused on resolving the Sea Lice parasitism (C. rogercresseyi) through the use of antibiotics to control the infestation and the use of vaccines to prevent it, but it presents biological implications for salmon, the marine environment and human health.

An Evaluation Of Antiparasitics Used To Treat The Sea Louse, Caligus Rogercresseyi, In Chilean Salmonid Farming, Dayla Woller

Scripps Senior Theses

Sea lice pose a global threat to open-net salmonid farming. As Chile is the second largest Atlantic salmon producer, they have a vested interest in mitigating infestations and increasing production. However, this will not be possible without 1) increased research on the underlying mechanisms behind sea lice infestations that will inform 2) governmental regulation of salmon farming. This increase in research and governmental regulation will only be possible through transparency on pesticide practices and sea louse monitoring data from companies operating in Chile. Taking these steps would bring Chile closer to being the largest producer of Atlantic salmon and an …

Effects Of Dietary Thiaminase On Reproductive Traits In Three Populations Of Atlantic Salmon Targeted For Reintroduction Into Lake Ontario, Kimberly T. Mitchell

Electronic Thesis and Dissertation Repository

The fitness of reintroduced salmonids in Lake Ontario can be reduced by high levels of thiaminase in exotic prey consumed at the adult stage. If sensitivity to dietary thiaminase differs among the three Atlantic salmon populations targeted for reintroduction into Lake Ontario, this could significantly influence their performance. I quantified the effects of experimental diets that contained high or low (control) levels of thiaminase on thiamine concentrations, survival, growth rate, and reproductive traits (sperm and egg quality) in Atlantic salmon from the three candidate source populations. Fish that consumed the high-thiaminase diet had comparable growth rates, but lower survival and …

Getting Over The Dam: Overcoming Institutional Barriers To The Recovery Of Atlantic Salmon By Navigating The Social-Science/Policy Interface, Melissa E. Flye

Electronic Theses and Dissertations

The term governance has undergone somewhat of an evolution since its inception, originally describing the act of governing, it has come to represent a more collaborative form of governing which is distinct from hierarchical control models (Marin and Mayntz, 1991). Collaborative governance refers to the systems associated with public policy decision making and resource management which span the jurisdictional boundaries of public agencies, levels of government, and/or public and private spheres in order to pursue a public policy goal or outcome (Emerson et al., 2012). Environmental management is often considered an inherently collaborative effort, as ecological systems and species rarely …

Risk Assessment Of Catch And Release, Rolf Erik Olsen, Tor Fredrik Næsje, Trygve Poppe, Lynne Sneddon, John Webb

Lynne Sneddon, PhD

The report was produced during most of 2009, and gives a state of art overview of current knowledge on the effects of catch and release practices on these fish species’ welfare, using accessible and peer reviewed published literature as basis for the assessment. Anecdotic and non-published reports have been used to a limited extent as they are regarded as untested or containing unverified statements. The Panel on Animal Health and Welfare discussed the full report in a meeting on the 9th of December, and gave its support to the conclusions drawn by the ad hoc-group. The report has concentrated on …

Multi-Instrument Evaluation Of A Real-Time Pcr Assay For Identification Of Atlantic Salmon: A Case Study On The Use Of A Pre-Packaged Kit For Rapid Seafood Species Identification, Amanda A. Naaum, Rosalee S. Hellberg, Tara A. Okuma, Robert H. Hanner

Food Science Faculty Articles and Research

Protecting the seafood supply chain from species substitution is critical for economic, health, and conservation reasons. DNA-based methods represent an effective means to detect species substitution, but current methods can be time consuming or costly, and require specialized instruments and operators. Real-time PCR provides an alternative that can be performed quickly, and in some cases even on-site. The use of commercial kits reduces the expertise required by the operator and therefore increases accessibility to testing. This potentially increases the likelihood of adoption into the supply chain, but only if the kits are robust across multiple operators, instruments, and samples. In …

Comparing The Effects Of Swimming Exercise And Dissolved Oxygen On Important Performance Parameters Of Early-Rearing Atlantic Salmon Salmo Salar And Rainbow Trout Oncorhynchus Mykiss., Thomas B. Waldrop

Graduate Theses, Dissertations, and Problem Reports

Swimming exercise (bodylenghts/sec), and dissolved oxygen (DO), are important environmental variables in aquaculture. While there is an obvious physiological association between these two parameters, their interaction has not been adequately studied in Atlantic salmon, Salmo salar and Rainbow trout, Oncorhynchus mykiss. Since both variables can be easily manipulated in modern aquaculture systems, we sought to assess the impact of these parameters, alone and in combination, on the performance, health, and welfare parameters of the two species. Atlantic salmon and Rainbow trout were stocked into twelve circular 0.5 m³ tanks and exposed to either high (1.5-2 BL/s) or low …

Antioxidants In Atlantic Salmon On A Diurnal Basis, Alexa Grissinger

Honors College

Atlantic salmon is a common aquaculture species that is now greatly impacted by sea lice and amoebic gill disease. Currently, one of the treatments uses hydrogen peroxide (H2O2) because it breaks down safely in water, leaving no toxic residues like some of the previous treatments. Hydrogen peroxide was an effective treatment previously, but now resistance seems to be developing amongst the disease organisms and, if too high a dose is used, it can harm the salmon. Antioxidants, specifically superoxide dismutase (SOD), catalase (CAT), and both independent and dependent glutathione peroxidase (GPx), are present in organisms to break down reactive oxygen …

Maine Infectious Salmon Anemia Virus Control Program Standards, Christopher Bartlett

Maine Sea Grant Publications

The United States Department of Agriculture, (USDA), Animal and Plant Health Inspection Services, (APHIS), Veterinary Services, (VS) Infectious Salmon Anemia Virus Control Program Standards, hereafter referred to as the Standards, establish recommended procedures for the prevention and containment of Infectious Salmon Anemia virus (ISAV) in farm-raised Atlantic salmon (Salmo salar). The Standards provide guidelines for producers, APHIS-accredited veterinarians, other fish health personnel, laboratory personnel, and regulators. The eligibility of Atlantic salmon producers affected by ISA in Maine to receive indemnity is based, in part, on compliance with these Standards, and upon availability of any indemnity funds …

Penobscot River Habitat Focus Area 2016 Annual Report, Matthew Bernier

Maine Sea Grant Publications

1 Penobscot River Habitat Focus Area 2016 Annual Report The Penobscot River is New England’s second largest river, draining nearly one-third of the state of Maine with a watershed area of 8,570 square miles not including tributaries to Penobscot Bay. The watershed is home to 11 migratory fish species, including three listed under the Endangered Species Act, and represents the largest run of Atlantic salmon in the United States. It has a rich cultural history of commercial, recreational, and sustenance fishing. It is also home to the Penobscot Indian Nation and includes the Katahdin Woods and Waters National Monument. The …

Evaluation Of Antibody Response To An Adjuvanted Hapten-Protein Vaccine As A Potential Inhibitor Of Sexual Maturation For Farmed Atlantic Salmon, Darcy S.O. Mora, Mo D. Salman, Christopher A. Myrick, Jack C. Rhyan, Lowell A. Miller, Ellen Marie Sætre, Douglas C. Eckery

USDA Wildlife Services: Staff Publications

An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated with one of four immunostimulants in a water-in-oil emulsion. Specific antibody responses to the peptide and each carrier protein were evaluated. While the antibody response to KLH was stronger than the response to BSA, both carrier proteins stimulated comparable antibody responses to the LH peptide. The immunostimulant proved to be more important for enhancing the LH peptide antibody response …

Innate And Learned Predator Recognition In Four Strains Of Captive-Bred Atlantic Salmon, Salmo Salar, Malcolm J. Lau

Electronic Thesis and Dissertation Repository

Innate and learned anti-predator responses can be important determinants of survival in natural environments. However, few studies have examined population differences in these anti-predator responses. My study measured innate and learned anti-predator responses in four strains of Atlantic salmon, Salmo salar, which had varying captive breeding histories. All four strains of salmon tested had an innate anti-predator response to alarm cue and no response to a visual predator cue. Following training in which the alarm cue and predator cue were paired, I found that one of the four strains (Sebago), developed a learned anti-predator responses as indicated by reduced …

Combining Modified Atmosphere Packaging And Nisin To Extend The Shelf Life Of Atlantic Salmon (Salmo Salar), Dong Han

All Theses

Atlantic salmon is often used in laboratory experiments to represent marine fishes. Many researchers try to optimize the levels and dosage of preservatives to maintain the freshness and extend the shelf-life of Atlantic salmon. Little information has been provided about the effectiveness of combined method between MAP and nisin, especially on the preservation of Atlantic salmon. Strong evidences suggest that MAP associated with nisin may have a potential to improve Atlantic salmon storage quality. Preservation effectiveness of combining modified atmosphere package (MAP) and nisin on fresh Atlantic salmon was determined using various methods. Six groups of farmed Atlantic salmon were …

Salmon Aquaculture / Salmoniculture, Downeast Fisheries Trail, Roosevelt-Campobello International Park

Maine Sea Grant Publications

This artist’s rendition of a single salmon pen shows how the fish are contained. Groups of pens are tied together by an underwater grid system of anchors, chains, and ropes.

How The Presidents Ate Their Salmon, Catherine Schmitt

Catherine Schmitt

No abstract provided.

Growth Of Atlantic Salmon (Salmo Salar) In Freshwater, Douglas Bradlee Sigourney

Open Access Dissertations

Growth plays a key role in regulating ecological and population dynamics. Life history characteristics such as age at maturity, fecundity and age and size at migration are tightly linked to growth rate. In addition, size can often determine survival and individual breeding success. To fully understand the process of growth it is important to understand the mechanisms that drive growth rates. In Atlantic salmon, growth is critical in determining life history pathways. Models to estimate growth could be useful in the broader context of predicting population dynamics. In this dissertation I investigate the growth process in juvenile Atlantic salmon (Salmo …

Risk Assessment Of Catch And Release, Rolf Erik Olsen, Tor Fredrik Næsje, Trygve Poppe, Lynne Sneddon, John Webb

Aquaculture and Fisheries Collection

The report was produced during most of 2009, and gives a state of art overview of current knowledge on the effects of catch and release practices on these fish species’ welfare, using accessible and peer reviewed published literature as basis for the assessment. Anecdotic and non-published reports have been used to a limited extent as they are regarded as untested or containing unverified statements. The Panel on Animal Health and Welfare discussed the full report in a meeting on the 9th of December, and gave its support to the conclusions drawn by the ad hoc-group.

The report has concentrated on …

Engaging Recreational Fishers In Management And Conservation: Global Case Studies, Elise F. Granek, Elizabeth M.P. Madin, M. A. Brown, Will F. Figueira, Darren S. Cameron, Zeb Hogan, Gerry Kristianson, Pierre De Villiers, Jack E. Williams, John R. Post, S. Zahn, R. Arlinghaus

Environmental Science and Management Faculty Publications and Presentations

Globally, the number of recreational fishers is sizeable and increasing in many countries. Associated with this trend is the potential for negative impacts on fish stocks through exploitation or management measures such as stocking and introduction of non-native fishes. Nevertheless, recreational fishers can be instrumental in successful fisheries conservation through active involvement in, or initiation of, conservation projects to reduce both direct and external stressors contributing to fishery declines. Understanding fishers’ concerns for sustained access to the resource and developing methods for their meaningful participation can have positive impacts on conservation efforts. We examined a suite of case studies that …

Endocrine Disruption In Atlantic Salmon (Salmo Salar) Exposed To Pesticides, Benjamin W. Spaulding

Electronic Theses and Dissertations

Since the early 19801s, the numbers of adult Atlantic salmon (Salmo salar) returning to Maine's rivers have been in a general decline. In addition, estimates of parr, freshwater smolt and emigrating smolt populations indicate low overwinter survival rates. Overall, these low numbers, along with several other factors, resulted in the Atlantic salmon in eight rivers in Maine being classified as a distinct population segment under the Endangered Species Act. Because many of the listed rivers are found near lowbush blueberry barrens, I investigated the endocrine disrupting potential and effects of selected blueberry pesticides on Atlantic salmon. An E-SCREEN assay was …

A Biochemical Study Of Mucus Lysomzyme, Proteins And Plasma Thyroxine Of Atlanctic Salmon (Salmo Salar) During Smoltification, Maresa Fagan

Masters

Atlantic salmon prepare to migrate from fresh water to sea water during a phase of great physiological change known as smoltification. In the context of stock enhancement programmes and the conservation of Atlantic salmon stocks, the release of smolts from freshwater to sea is of major significance. Accordingly, this study set out to monitor some of the physiological and biochemical changes, which allow the Atlantic salmon to migrate from freshwater to seawater. Our research focused on skin mucus as a possible candidate for the development of a non invasive test and sought to elucidate biochemical or significant protein changes in …

A Biochemical Study Of Atlantic Salmon (Salmo Salar) Skin Mucus Proteins During Smoltification (1999 And 2001), Paul Kennedy

Masters

Smoltification is a complex process which involves morphological, physiological and behavioural changes which allow Atlantic salmon to migrate from fresh water to seawater. The biological interface between the salmon and their environment is the mucus coat, which is composed of a wide variety of biochemical secretions primarily from goblet cells on the surface of the epithelial layers, but also other cells such as sacciform cells and acidophilic granular cells. Skin mucus has been implicated in a diversity of roles including respiration, osmoregulation, reproduction, excretion, disease resistance, communication, feeding and protection ( Shepard, 1994). Further investigation is warranted with regard to …

Environmental Enrichment And Prior Experience Of Live Prey Improve Foraging Behaviour In Hatchery-Reared Atlantic Salmon, C. Brown, T. Davidson, K. Laland

Aquaculture Collection

Atlantic salmon salmo salar L. parr were reared for 3 months under standard hatchery conditions or in a structurally enriched tank (containing plants, rocks and novel objects). Half of each of these fish had prior exposure to live prey in the form of live bloodworm while the other half were fed hatchery-pellets. After 12 days all fish were tested on a novel live prey item (brine shrimp). A significant interaction between the two factors (prior exposure to live prey and rearing condition) revealed that foraging performance was only enhanced in fish that had been reared in a complex environment and …

Social Learning Of Prey Location In Hatchery-Reared Atlantic Salmon, C. Brown, A. Markula, K. Laland

Aquaculture Collection

Naïve, hatchery-reared Atlantic salmon Salmo salar parr were paired with demonstrators that had been pre-trained to accept live prey from the surface or from the benthos. After 6 days of observing demonstrators through a clear perspex partition the naïve fish’s benthic foraging skills were tested. The results revealed that hatchery-reared Atlantic salmon can be taught to target benthic prey items by observation alone and social learning protocols can be utilized to dramatically increase benthic foraging success. The results are discussed with reference to refining hatchery-rearing practices with a view to improving the post-release survival of hatchery fishes. The role of …

Best Practices In Endangered Species Recovery Planning: Lessons For The Conservation Of Maine’S Atlantic Salmon, Raymond J. O’Connor, Ray B. Owen, Judith Rhymer

Maine Policy Review

The call for federal listing of Atlantic salmon as endangered implies that such action will result in a recovery plan for the species that is superior to Maine ’s Atlantic Salmon Conservation Plan. In this article the authors compare the Maine plan against the findings of a recent review of Endangered Species Act recovery plans. The review, conducted by the Society for Conservation Biology in collaboration with the U.S. Fish and Wildlife Service, assessed the quality of a national sample of Endangered Species Act recovery plans with the intention of identifying “best practice.” By comparing the Maine plan to the …