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Full-Text Articles in Life Sciences
Population And Demographic Structure Of Ixodes Scapularis Say In The Eastern United States., Joyce M. Sakamoto, Jerome Goddard, Jason L. Rasgon
Population And Demographic Structure Of Ixodes Scapularis Say In The Eastern United States., Joyce M. Sakamoto, Jerome Goddard, Jason L. Rasgon
College of Agriculture & Life Sciences Publications and Scholarship
INTRODUCTION: The most significant vector of tick-borne pathogens in the United States is Ixodes scapularis Say (the blacklegged tick). Previous studies have identified significant genetic, behavioral and morphological differences between northern vs. southern populations of this tick. Because tick-borne pathogens are dependent on their vectors for transmission, a baseline understanding of the vector population structure is crucial to determining the risks and epidemiology of pathogen transmission. METHODS: We investigated population genetic variation of I. scapularis populations in the eastern United States using a multilocus approach. We sequenced and analyzed the mitochondrial COI and 16S genes and three nuclear genes (serpin2, …
Quantitative Field Testing Heterodera Glycines From Metagenomic Dna Samples Isolated Directly From Soil Under Agronomic Production., Yan Li, Gary W. Lawrence, Shien Lu, Clarissa Balbalian, Vincent P. Klink
Quantitative Field Testing Heterodera Glycines From Metagenomic Dna Samples Isolated Directly From Soil Under Agronomic Production., Yan Li, Gary W. Lawrence, Shien Lu, Clarissa Balbalian, Vincent P. Klink
College of Agriculture & Life Sciences Publications and Scholarship
A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.
Magnitude Of The Freshwater Turtle Exports From The Us: Long Term Trends And Early Effects Of Newly Implemented Harvest Management Regimes., Ivana Mali, Michael W. Vandewege, Scott K. Davis, Michael R.J. Forstner
Magnitude Of The Freshwater Turtle Exports From The Us: Long Term Trends And Early Effects Of Newly Implemented Harvest Management Regimes., Ivana Mali, Michael W. Vandewege, Scott K. Davis, Michael R.J. Forstner
College of Agriculture & Life Sciences Publications and Scholarship
Unregulated commercial harvest remains a major threat for turtles across the globe. Due to continuing demand from Asian markets, a significant number of turtles are exported from the United States of America (US). Beginning in 2007, several southeastern states in the US implemented restrictions on the commercial harvest of turtles, in order to address the unsustainable take. We have summarized freshwater turtle exports from the US between 2002 and 2012 and demonstrated that the magnitude of turtle exports from the US remained high although the exports decreased throughout the decade. Louisiana and California were the major exporters. The majority of …
Fecal Near Infrared Spectroscopy To Discriminate Physiological Status In Giant Pandas., Erin E. Wiedower, Andrew J. Kouba, Carrie K. Vance, Rachel L. Hansen, Jerry W. Stuth, Douglas R. Tolleson
Fecal Near Infrared Spectroscopy To Discriminate Physiological Status In Giant Pandas., Erin E. Wiedower, Andrew J. Kouba, Carrie K. Vance, Rachel L. Hansen, Jerry W. Stuth, Douglas R. Tolleson
College of Agriculture & Life Sciences Publications and Scholarship
Giant panda (Ailuropoda melanoleuca) monitoring and research often require accurate estimates of population size and density. However, obtaining these estimates has been challenging. Innovative technologies, such as fecal near infrared reflectance spectroscopy (FNIRS), may be used to differentiate between sex, age class, and reproductive status as has been shown for several other species. The objective of this study was to determine if FNIRS could be similarly used for giant panda physiological discriminations. Based on samples from captive animals in four U.S. zoos, FNIRS calibrations correctly identified 78% of samples from adult males, 81% from adult females, 85% from adults, 89% …