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Impact Of Ros Presence On Oncogenic Ras Activity, Chris Andersen
Impact Of Ros Presence On Oncogenic Ras Activity, Chris Andersen
Summer Research
Previous research has suggested a connection between oncogenic Ras and the cell’s levels of Reactive Oxygen Species (ROS). The underlying cellular mechanism is not well understood. To investigate this connection, we applied the UAS-GAL4 system in Drosophila melanogaster flies to control the expression of Ras and Keap1, a key redox regulator.2 We expected the activity of Ras to vary with its redox environment and thus impact protein activity downstream of Ras signaling cascades. In monitoring three proteins downstream of Ras—Dcp-1, Akt, and MAPK—we aimed to determine which pathways were impacted by ROS modulation.
Using Crispr To Induce A Knock-Out Of Dprl-1 In Drosophila Melanogaster, Alicia Walker
Using Crispr To Induce A Knock-Out Of Dprl-1 In Drosophila Melanogaster, Alicia Walker
Summer Research
Phosphatase of regenerating liver (PRL) is a protein that controls cell processes such as growth and division which has unknown targets. PRL has been found to have both oncogenic and tumor suppressive properties. This study aimed to create a knock out of PRL in Drosohpila melanogaster in order to assess its role in development and in order to illuminate its activity when it is expressed in cancers. We hypothesize that dPRL-1 plays an important role in embryogenesis and that the progeny which lack this gene will be unviable. The CRISPR/Cas9 system was selected as the method in which to create …