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Articles 1 - 30 of 95
Full-Text Articles in Life Sciences
Alteration In The Subcellular Location Of The Inhibitor Of Growth Proteinp33(Ing1b) In Estrogen Receptor Alpha Positive Breast Carcinoma Cells, İmge Kunter, Emi̇ne Kandemi̇ş, Hani̇ Alotai̇bi̇, Tülay Canda, Şeri̇fe Esra Erdal Bağriyanik
Alteration In The Subcellular Location Of The Inhibitor Of Growth Proteinp33(Ing1b) In Estrogen Receptor Alpha Positive Breast Carcinoma Cells, İmge Kunter, Emi̇ne Kandemi̇ş, Hani̇ Alotai̇bi̇, Tülay Canda, Şeri̇fe Esra Erdal Bağriyanik
Turkish Journal of Biology
ING1 has regulatory roles in the expression of genes associated with proliferation, apoptosis, and senescence. p33(ING1b) is the most widely expressed isoform of the gene. Downregulation of its nuclear expression is involved in differentiation and pathogenesis in invasive breast carcinoma. Yet the mechanism(s) by which p33 nuclear targeting is regulated remains unknown. In this study, we analyzed human invasive breast carcinoma tissue samples by immunostaining with p33 and correlating p33 location with the presence of ERα. Our findings show the expression of p33 protein in ERα-positive tumor samples was in the nucleus alone, while the expression was mainly in the …
Amperometric Biosensor For Detection Of Triglyceride Tributyrinbased On Zero Point Charge Of Activated Carbon, Mohsen Rezvani, Ghasem Najafpour, Maedeh Mohammadi, Hossein Zare
Amperometric Biosensor For Detection Of Triglyceride Tributyrinbased On Zero Point Charge Of Activated Carbon, Mohsen Rezvani, Ghasem Najafpour, Maedeh Mohammadi, Hossein Zare
Turkish Journal of Biology
A simple and robust single enzyme biosensor was fabricated for triglyceride (TG) detection. Graphite rods were modified with activated carbon (AC) and used as support for lipase immobilization. Chitosan (CHIT) was eventually used to create film on the bioelectrode and retain the immobilized enzyme. To extend the linear range of TG detection, AC was functionalized with carboxyl and then amine groups (AAC) to enhance the isoelectric point of AC. The constructed graphite/AAC/lipase /CHIT bioelectrode was characterized using cyclic voltammetry and field emission scanning electron microscopy (FE-SEM). The accuracy of the developed biosensor was assessed through determination of different concentrations of …
Optimization, Purification, And Characterization Of An Extracellular Antifungal Protein From Serratia Marcescens Dt3 Isolated From Soil In Vietnam, Tuyen Thi Do, Thao Thi Nguyen, Thanh Sy Le Nguyen, Hoang Thanh Le
Optimization, Purification, And Characterization Of An Extracellular Antifungal Protein From Serratia Marcescens Dt3 Isolated From Soil In Vietnam, Tuyen Thi Do, Thao Thi Nguyen, Thanh Sy Le Nguyen, Hoang Thanh Le
Turkish Journal of Biology
Gram-negative Serratia marcescens is a potential biological control agent of fungal plant diseases in many tropical regions. Its antifungal activity is due to the production of prodigiosin, protease, chitinase, and other substances that act to weaken the fungal cell wall and cause lysis. In the following investigation, a novel extracellular protein with antifungal activity against Rhizoctonia solani and Fusarium oxysporum was optimally produced, purified, and characterized from a native Serratia marcescens DT3. Under optimal conditions (medium containing 1.25% peptone, 0.5% yeast extract; temperature of 28 °C; initial pH of 7; culture time of 28 h), the antifungal activity of the …
Induction Of Haploid Plants In Citrus Through Gamma-Irradiated Pollen And Ascertainment Of Ovule Age For Maximum Recovery Of Haploid Plantlets, Manoj Kundu, Anil Dubey, Manish Srivastav, Surendra Kumar Malik
Induction Of Haploid Plants In Citrus Through Gamma-Irradiated Pollen And Ascertainment Of Ovule Age For Maximum Recovery Of Haploid Plantlets, Manoj Kundu, Anil Dubey, Manish Srivastav, Surendra Kumar Malik
Turkish Journal of Biology
The present investigation was carried out for the induction of haploid plants in Citrus grandis through in situ parthenogenesis by pollination with gamma-irradiated pollen of C. limetta and C. sinensis, treated with 50, 100, 200, 300, and 400 Gy gamma ray doses followed by in vitro ovule culture. Ovule culture at 50 days after pollination (DAP) was found optimum for the maximum recovery of in vitro-raised plantlets as compared to 35 and 20 DAP. At 50 DAP, irrespective of the pollen parent, plantlet regeneration capacity decreased at 300 and 400 Gy irradiation treatment (0.62% and 0.60%, respectively) with maximum recovery …
Comparative Phytotoxicity Of Undoped And Er-Doped Zno Nanoparticles Onlemna Minor L.: Changes In Plant Physiological Responses, Samaneh Torbati, Alireza Khataee, Shabnam Saadi
Comparative Phytotoxicity Of Undoped And Er-Doped Zno Nanoparticles Onlemna Minor L.: Changes In Plant Physiological Responses, Samaneh Torbati, Alireza Khataee, Shabnam Saadi
Turkish Journal of Biology
The present study is the first research on the phytotoxicological effects of Er-doped zinc oxide nanoparticles (NPs) on duckweed (Lemna minor L.), as a model aquatic floating macrophyte. It concentrated on the comparison of the physiological effects of different concentrations of undoped and Er-doped zinc oxide NPs on L. minor. Pure and Er-doped zinc oxide samples were synthesized through a sonochemical method and their morphology and chemical composition were studied by scanning electron microscopy and energy dispersive X-ray analysis. Plant growth, photosynthetic pigment contents, and antioxidant enzyme activities were investigated as indices to assess the effects and toxicity of the …
Aggregation Of A Parthenogenetic Diploid Embryo And A Male Embryo Improves The Blastocyst Development And Parthenogenetic Embryonic Stem Cell Derivation, Xiaoyan Qiu, Nan Li, Xiong Xiao, Yuemin Li
Aggregation Of A Parthenogenetic Diploid Embryo And A Male Embryo Improves The Blastocyst Development And Parthenogenetic Embryonic Stem Cell Derivation, Xiaoyan Qiu, Nan Li, Xiong Xiao, Yuemin Li
Turkish Journal of Biology
Parthenogenetically derived mammalian embryos, with no paternal genome, are not viable and die, largely from defective placental growth attributed to a lack of paternal effect, resulting in the low blastulation rate and low derivation efficiency of parthenogenetic embryonic stem cells (pESCs). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo and the identified male embryo, aims to investigate a method to improve the development of parthenogenetic embryo and pESC derivation in mice. Using different chemical combinations for the optimization, we found that the heterozygous diploid type had a significantly higher …
In Vitro Evaluation Of Plla/Pbs Sponges As A Promisingbiodegradable Scaffold For Neural Tissue Engineering, İrem Ayşe Kanneci̇ Altinişik, Fatma Neşe Kök, Deni̇z Yücel, Gamze Köse
In Vitro Evaluation Of Plla/Pbs Sponges As A Promisingbiodegradable Scaffold For Neural Tissue Engineering, İrem Ayşe Kanneci̇ Altinişik, Fatma Neşe Kök, Deni̇z Yücel, Gamze Köse
Turkish Journal of Biology
In tissue engineering, the use of poly-L-lactic acid (PLLA)/polybutylene succinate (PBS) blend for the construction of scaffold is very limited. Moreover, polymeric sponges fabricated from PLLA/PBS have not been studied for neural tissue engineering. In the present study, the potential of the utility of PLLA/PBS polymeric sponges seeded with Schwann cells was investigated. PLLA and PBS were blended in order to increase the processability and tune the crystallinity, porosity, and degradation rate of the resulted polymeric sponges. These sponges were then seeded with Schwann cells. Porosity analysis showed that there were no significant differences between different compositions of PLLA/PBS blends; …
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Turkish Journal of Biology
Detailed knowledge of the three-dimensional (3D) structure of a protein is essential for the proper understanding of its function(s) that could be modified through posttranslational modifications (PTMs). Among these PTMs, alterations of serine/threonine residues of a protein through phosphorylation and O-glycosylation are extremely dynamic and could modulate the functions of a protein by affecting their 3D structure. Potential of a protein for certain PTMs could be evaluated through computer-based methods. Erythropoietin (EPO) is a multifunctional protein that primarily regulates red blood cell production and is also involved in other nonhematopoietic functions; for example, EPO also has cardioprotective and neuroprotective effects. …
Tumor Necrosis Factor-Alpha Induced Caspase-3 Activation-Related Inos Gene Expression In Adp-Activated Platelets, Özge Çevi̇k, Zelal Adigüzel, Ahmet Tarik Baykal, Azi̇ze Şener
Tumor Necrosis Factor-Alpha Induced Caspase-3 Activation-Related Inos Gene Expression In Adp-Activated Platelets, Özge Çevi̇k, Zelal Adigüzel, Ahmet Tarik Baykal, Azi̇ze Şener
Turkish Journal of Biology
Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-α in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-α and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding; cGMP, Cyt-c, and Ca2+ levels and NOS activation; and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-α and/or 50 mM iNOS …
Expression Of The Zmdef1 Gene And α-Amylase Inhibitory Activityof Recombinant Defensin Against Maize Weevils, Thi Xuan Thuy Vi, Hoang Duc Le, Vu Thanh Thanh Nguyen, Van Son Le, Hoang Mau Chu
Expression Of The Zmdef1 Gene And α-Amylase Inhibitory Activityof Recombinant Defensin Against Maize Weevils, Thi Xuan Thuy Vi, Hoang Duc Le, Vu Thanh Thanh Nguyen, Van Son Le, Hoang Mau Chu
Turkish Journal of Biology
Plant defensins are multifunctional small cysteine-rich proteins. They are active against fungi, bacteria, and many viruses, and they inhibit trypsin and α-amylase activities. In this study, we expressed the maize defensin gene (ZmDEF1) in tobacco seeds in order to establish the basis for generating transgenic maize plants resistant to weevils. The ZmDEF1 gene was isolated from Maison, a Vietnamese local maize cultivar, which is well known for having the highest resistance to weevils among other local cultivars. The ZmDEF1 gene was cloned into a binary vector, pBetaPhaso-dest, which carries phaseolin, a seed-specific promoter, to direct defensin expression in tobacco seeds. …
Population Variation In Drought Resistance And Its Relationship With Adaptiveand Physiological Seedling Traits In Turkish Red Pine (Pinus Brutia Ten.), Gaye Kandemi̇r, Sertaç Önde, Fati̇h Temel, Zeki̇ Kaya
Population Variation In Drought Resistance And Its Relationship With Adaptiveand Physiological Seedling Traits In Turkish Red Pine (Pinus Brutia Ten.), Gaye Kandemi̇r, Sertaç Önde, Fati̇h Temel, Zeki̇ Kaya
Turkish Journal of Biology
Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the relationship between drought damage and physiological traits (i.e. plant moisture stress and proline content). Three-year-old seedlings were subjected to drought treatment during the summer of 2000 …
Genetic Engineering Of An Industrial Strain Of Streptomyces Clavuligerusfor Further Enhancement Of Clavulanic Acid Production, Aslihan Kurt Kizildoğan, Güli̇z Vanli Jaccard, Alper Mutlu, İbrahi̇m Sertdemi̇r, Gülay Özcengi̇z
Genetic Engineering Of An Industrial Strain Of Streptomyces Clavuligerusfor Further Enhancement Of Clavulanic Acid Production, Aslihan Kurt Kizildoğan, Güli̇z Vanli Jaccard, Alper Mutlu, İbrahi̇m Sertdemi̇r, Gülay Özcengi̇z
Turkish Journal of Biology
An industrial clavulanic acid (CA) overproducer Streptomyces clavuligerus strain, namely DEPA, was engineered to further enhance its CA production. Single or multiple copies of ccaR, claR (pathway-specific activators), and cas2 (CA synthase) genes under the control of different promoters were introduced into this strain. CA titers of the resulting recombinants were analyzed by HPLC in a dynamic fashion and compared to the vector-only controls and a wild-type strain of S. clavuligerus while their growth was monitored throughout fermentation. The addition of an extra copy of ccaR, under control of its own promoter or constitutive ermE* promoter (PermE*), led to 7.6- …
Improvement In Androgenic Response Of Borage (Borago Officinalis L.) Cultured Anthersusing Antibrowning Agents And Picloram, Mohammad Reza Abdollahi, Zahra Chardoli Eshaghi, Mohammad Majdi
Improvement In Androgenic Response Of Borage (Borago Officinalis L.) Cultured Anthersusing Antibrowning Agents And Picloram, Mohammad Reza Abdollahi, Zahra Chardoli Eshaghi, Mohammad Majdi
Turkish Journal of Biology
Given the importance of borage (Borago officinalis L.) as a medicinal herb, androgenic studies are necessary in order to speed up the breeding programs of this plant species. In this work, we evaluated the effect of silver nitrate, activated charcoal, ascorbic acid, and polyvinylpyrrolidone (PVP), previously described as antibrowning agents and promoters of androgenesis. Adding silver nitrate at a concentration of 5 mg L-1 significantly enhanced the androgenic responses of cultured anthers up to ~2-fold for total calli production (53.33%) and up to ~2.5-fold for embryonic calli and embryo formation (30% and 0.3 embryos/anther, respectively) compared to control culture medium. …
Morphology, Development, And Transplant Potential Of Prunus Aviumand Cornus Sanguinea Seedlings Growing Under Different Led Lights, Filippos Bantis, Kalliopi Radoglou
Morphology, Development, And Transplant Potential Of Prunus Aviumand Cornus Sanguinea Seedlings Growing Under Different Led Lights, Filippos Bantis, Kalliopi Radoglou
Turkish Journal of Biology
The objective of the present study was to investigate the impact of light-emitting diodes (LEDs) on the morphological and developmental characteristics of wild cherry (Prunus avium) and common dogwood (Cornus sanguinea). The LEDs used were L20AP67 (moderate blue, red and far-red, high green), AP673L (moderate blue, high red), G2 (low blue, high red and far-red), AP67 (moderate blue, red and far-red), and NS1 (high blue and green, low red, high red:far-red, 1% ultraviolet). Fluorescent light [FL (high blue and green, low red)] tubes served as the control treatment. The growth rate and subsequently the shoot height of Cornus sanguinea were …
Role Of Snf5 In Rheumatoid Arthritis By Upregulation Ofp16 And Inactivation Of Jnk Pathway, Shupeng Wu, Jing Wang, Fang Li
Role Of Snf5 In Rheumatoid Arthritis By Upregulation Ofp16 And Inactivation Of Jnk Pathway, Shupeng Wu, Jing Wang, Fang Li
Turkish Journal of Biology
This study aims to explore the role and the possible underlying molecular mechanism of SNF5 in the pathogenesis of rheumatoid arthritis (RA). MH7A cells were respectively transfected with pc-SNF5 (pcDNA3.1 containing the Brg1 coding sequence), short hairpin RNA against SNF5 (shSNF5), and their negative controls (pcDNA3.1 and shNC). The alterations of SNF5 expression were assessed by qRT-PCR and western blot analysis. MTT assay, flow cytometry, and western blot analysis were performed to evaluate proliferation, apoptosis, and expression levels of p16 and JNK pathway associated proteins, respectively. Finally, the effect of SNF5 was verified in fibroblast-like synoviocytes (FLSs) obtained from a …
Application Of Proline To Root Medium Is More Effective For Amelioration Of Photosynthetic Damages As Compared To Foliar Spraying Or Seed Soaking In Maize Seedlings Under Short-Term Drought, Mehmet Demi̇ralay, Cansu Altuntaş, Asi̇ye Sezgi̇n, Rabi̇ye Terzi̇, Asi̇m Kadioğlu
Application Of Proline To Root Medium Is More Effective For Amelioration Of Photosynthetic Damages As Compared To Foliar Spraying Or Seed Soaking In Maize Seedlings Under Short-Term Drought, Mehmet Demi̇ralay, Cansu Altuntaş, Asi̇ye Sezgi̇n, Rabi̇ye Terzi̇, Asi̇m Kadioğlu
Turkish Journal of Biology
Exogenous proline (PRO) at low concentrations can enhance drought stress tolerance in different application modes such as application to rooting medium, foliar spray, and seed soaking. However, there is no information about which application mode is more effective for increasing the drought tolerance. Comparative effects of 1, 10, and 20 mM PRO applications through three application modes to hydroponically grown seedlings were examined under short-term drought stress in maize seedlings. Effects on leaf water potential, membrane damage, chlorophyll content, proline level, and gas exchange parameters such as net photosynthetic rate (Pn), transpiration rate (E), stomatal conductance (gs), and substomatal CO2 …
Silver Nanoparticles Induced Genotoxicity And Oxidative Stress In Tomato Plants, Fazi̇let Özlem Çeki̇ç, Sefa Eki̇nci̇, Müslüm Süleyman İnal, Di̇lek Özakça
Silver Nanoparticles Induced Genotoxicity And Oxidative Stress In Tomato Plants, Fazi̇let Özlem Çeki̇ç, Sefa Eki̇nci̇, Müslüm Süleyman İnal, Di̇lek Özakça
Turkish Journal of Biology
Among nanoparticles, silver nanoparticles (AgNPs) are intensively used in many materials owing to their antibacterial effects. In the present study different concentrations of AgNPs in Hoagland solution were applied to tomato seedlings. Total chlorophyll content, relative water content (RWC), antioxidant enzyme activities, and malondialdehyde content (MDA) as well as the genomic template stability (GTS) were analyzed. The intersimple sequence repeat polymerase chain reaction assay (ISSR-PCR) was used to determine the genotoxic effects of AgNPs on DNA. RWC did not change under AgNPs treatments; however, total chlorophyll content was significantly reduced by AgNPs applications. ISSR profiles demonstrated a consistent increase in …
Mesoporous Silica Nanoparticles Sba-15 Loaded With Emodin Upregulate The Antioxidative Defense Of Euproctis Chrysorrhoea (L.) Larvae, Milena Jankovic-Tomanic, Dajana Todorovic, Zoran Stanivukovic, Vesna Peric Mataruga, Ludger Wessjohann, Goran Kaludjerovic
Mesoporous Silica Nanoparticles Sba-15 Loaded With Emodin Upregulate The Antioxidative Defense Of Euproctis Chrysorrhoea (L.) Larvae, Milena Jankovic-Tomanic, Dajana Todorovic, Zoran Stanivukovic, Vesna Peric Mataruga, Ludger Wessjohann, Goran Kaludjerovic
Turkish Journal of Biology
The study presented here aims to elucidate the effects of emodin (EO = 1,3,8-trihydroxy-6-methylanthraquinone) in its free form and when loaded into a mesoporous silica nanocarrier SBA-15 (→ SBA-15 EO) on the activities of the main antioxidative enzymes, superoxide dismutase, catalase, glutathione S-transferase, and glutathione reductase, in larvae of a polyphagous insect pest, the browntail moth Euproctis chrysorrhoea (L.). The results show that only SBA-15 EO upregulates the activities of the tested antioxidative enzymes. These results point to significant differences in the effectiveness of the compound in the free versus the loaded form.
Stably Expressed Reference Genes During Differentiation Ofbone Marrow-Derived Mesenchymal Stromal Cells, Ilgin Cagnan, Fatima Aerts Kaya, Fahri̇ye Duygu Çeti̇nkaya, Ayşen Özcan
Stably Expressed Reference Genes During Differentiation Ofbone Marrow-Derived Mesenchymal Stromal Cells, Ilgin Cagnan, Fatima Aerts Kaya, Fahri̇ye Duygu Çeti̇nkaya, Ayşen Özcan
Turkish Journal of Biology
Human mesenchymal stromal cells (MSCs) from different parts of the body (i.e. bone marrow, BM) have distinct cellular and molecular features including global gene expression profiles. Quantitative polymerase chain reaction is a reliable method used in the quantification of gene expression. Correct assessment of target gene expression mostly depends on the reference gene (RG) of choice. Herein, expression levels of RGs (n = 19) in adipo-/osteogenic-differentiated and control (uninduced) BM-MSCs from donors were measured using the RealTime ready Human Reference Gene Panel. Characterization of BM-MSCs was assessed using in vitro differentiation by histochemical staining and immunophenotyping of cells by flow …
Cloning And Characterization Of A Serine/Threonine Protein Phosphatase2a-Encoding Gene Ibpp2a1 From Ipomoea Batatas (L.) Lam., Pan Xu, Bin Yong, Huan-Huan Shao, Jiabin Shen, Bin He, Qinqin Ma, Xianghua Yuan, Yu Wang
Cloning And Characterization Of A Serine/Threonine Protein Phosphatase2a-Encoding Gene Ibpp2a1 From Ipomoea Batatas (L.) Lam., Pan Xu, Bin Yong, Huan-Huan Shao, Jiabin Shen, Bin He, Qinqin Ma, Xianghua Yuan, Yu Wang
Turkish Journal of Biology
Sweet potato is one of the most important food crops with strong environmental adaptability. Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that plays crucial roles in hormone signal transduction and abiotic stress response. Characterization of PP2A can elucidate the mechanisms of stress resistance in this crop. In this study, a total of 15 PP2A transcripts, including nine regulatory subunit-encoding sequences and six catalytic subunit-encoding sequences, were identified from sweet potato and found to be expressed at varying levels. Only one contained a complete open reading frame and encoded a B regulatory subunit (termed IbPP2A1). Ten polymorphic sites …
Molecular Investigations Of The Somatic Embryogenesis Recalcitrancein The Cherry (Prunus Cerasus L.) Rootstock Cab 6p, Kaouther Ben Mahmoud, Emna Jedidi, Fabienne Delporte, Yordan Muhovski, Ahmed Jemmali, Philippe Druart
Molecular Investigations Of The Somatic Embryogenesis Recalcitrancein The Cherry (Prunus Cerasus L.) Rootstock Cab 6p, Kaouther Ben Mahmoud, Emna Jedidi, Fabienne Delporte, Yordan Muhovski, Ahmed Jemmali, Philippe Druart
Turkish Journal of Biology
Somatic embryogenesis is a powerful tool for clonal propagation and plant breeding in many plant species, but its application is limited by the genotype response based on the interaction between gene expression and culture conditions. In this context, the recalcitrance of the nonembryogenic cherry rootstock Prunus cerasus cultivar CAB 6P was investigated via the analysis of the candidate genes PiABP19, Picdc2, and PiSERK3 in comparison with the embryogenic genotype Prunus incisa cultivar N°131. Semiquantitative RT-PCR was employed to assess the transcript level of these genes in leaf explants and derived calli of the two genotypes cultured on picloram-containing MS medium …
Characterization And Pathogenicity Of Fusarium Proliferatum Andfusarium Verticillioides, Causal Agents Of Fusarium Ear Rot Of Corn, Nur Ain Izzati Mohd Zainudin, Farah Aqila Hamzah, Nor Azizah Kusai, Nur Syuhada Zambri, Suhaida Salleh
Characterization And Pathogenicity Of Fusarium Proliferatum Andfusarium Verticillioides, Causal Agents Of Fusarium Ear Rot Of Corn, Nur Ain Izzati Mohd Zainudin, Farah Aqila Hamzah, Nor Azizah Kusai, Nur Syuhada Zambri, Suhaida Salleh
Turkish Journal of Biology
Fusarium ear rot is a significant disease of corn caused by several toxigenic Fusarium species including Fusarium proliferatum and Fusarium verticillioides. Forty-one Fusarium isolates were recovered from corn with Fusarium ear rot disease symptoms collected from Peninsular Malaysia. Isolates were classified into three described species known as F. proliferatum, F. verticillioides, and F. solani. Based on sexual compatibility test, four isolates from F. proliferatum (MATD-1) were crossed-fertile with tester isolate F. proliferatum D024853 (MATD-2), producing perithecia in the presence of ascospores. Meanwhile, the isolates from F. verticillioides (MATA-2) were crossed with tester isolate F. verticillioides A00149 (MATA-1), but were found …
Cytotoxic And Apoptotic Effects Of Endemic Centaurea Fenzlii Reichardt On Themcf-7 Breast Cancer Cell Line, Ümi̇t Yirtici, Fati̇h Göger, Mehmet Sarimahmut, Aysun Ergene
Cytotoxic And Apoptotic Effects Of Endemic Centaurea Fenzlii Reichardt On Themcf-7 Breast Cancer Cell Line, Ümi̇t Yirtici, Fati̇h Göger, Mehmet Sarimahmut, Aysun Ergene
Turkish Journal of Biology
The main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. The goal of the analysis was to reveal the mechanism by which this effect occurs through researching the apoptotic side of these fractions and determining the amount of several proteins that are the products of the genes. Test substances were applied to breast cancer cells and the inhibitory concentration value 50 (IC50) that …
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
Turkish Journal of Biology
Tartary buckwheat is a type of coarse cereal that contains a rich supply of flavonoids. When suffering from certain stresses, tartary buckwheat can respond by promoting the biosynthesis of anthocyanins. It is well established that the jasmonate (JA) signaling pathway plays a critical role in plant resistance to disease by stimulating the production of anthocyanins; furthermore, the genes encoding the jasmonate-ZIM domain (JAZ) proteins have been shown to be key players in this process. However, the role of JAZ proteins in tartary buckwheat is poorly understood. In this study, we aimed to isolate FtJAZs from tartary buckwheat and identify FtJAZs …
A R2r3 Myb Transcription Factor From Ash Positively Regulates Salt Response In Tobacco, Tian Li, Jingkuan Sun, Yuping Bi
A R2r3 Myb Transcription Factor From Ash Positively Regulates Salt Response In Tobacco, Tian Li, Jingkuan Sun, Yuping Bi
Turkish Journal of Biology
The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). Phylogenetic analysis revealed that FvMYB2 was clustered with VvMYB60 from Vitis vinifera. PlantCARE software analysis showed that its promoter contained different cis elements involved in diverse abiotic stresses. The expression patterns of FvMYB2 were investigated under different abiotic stress conditions. FvMYB2 subcellular localization was mainly localized in the nucleus. Overexpression of FvMYB2 in tobacco showed enhanced salt resistance and upregulation of …
Accumulation Of Phenolics In Natural And Micropropagated Plantlets Of Thymus Pseudopulegioides Klokov & Des.-Shost. With Their Antioxidant Potentials, Mustafa Günaydin, Abdul Hafeez Laghari, Ersan Bektaş, Münevver Sökmen, Atalay Sökmen
Accumulation Of Phenolics In Natural And Micropropagated Plantlets Of Thymus Pseudopulegioides Klokov & Des.-Shost. With Their Antioxidant Potentials, Mustafa Günaydin, Abdul Hafeez Laghari, Ersan Bektaş, Münevver Sökmen, Atalay Sökmen
Turkish Journal of Biology
Thymus pseudopulegioides plantlets were propagated in vitro via direct organogenesis by using Murashige and Skoog (MS) media containing kinetin, thidiazuron, and 6-benzyladenine (BA) individually. Methanol extracts obtained both from plantlets and wild plants were analyzed for their total phenolics and flavonoid contents, then quantified by HPLC. The highest total phenolic (8.83 mg/g as gallic acid equivalent) and total flavonoid (0.92 mg/mL as rutin equivalent) values were from the MS media supplemented with 1.0 mg/L kinetin and 0.5 mg/L BA, respectively. The plantlets grown in those media also showed remarkable antioxidant activities with an IC50 value of 4.77 \mug/mL in DPPH …
Effect Of Mutation In Active Site Residue Trp209 To Val, Leu, Ile And Pro On The Catalytic Activity And Affinity For Some Benzenesulfonamides Of Human Carbonic Anhydrase Ii, Deryanur Kiliç, Orhan Erdoğan, Ömer İrfan Küfrevi̇oğlu
Effect Of Mutation In Active Site Residue Trp209 To Val, Leu, Ile And Pro On The Catalytic Activity And Affinity For Some Benzenesulfonamides Of Human Carbonic Anhydrase Ii, Deryanur Kiliç, Orhan Erdoğan, Ömer İrfan Küfrevi̇oğlu
Turkish Journal of Biology
Human carbonic anhydrase II (hCA II) enzyme was firstly expressed using a pET-SUMO expression vector in Escherichia coli and the recombinant enzyme was purified using nickel (Ni2+) affinity chromatography. The substitutions of Trp 209 with four amino acid (Val, Leu, Ile, and Pro) in the hydrophobic pocket of hCA II were conducted using site-directed mutagenesis. The p-nitrophenyl esterase activity of hCA II variants correlates with the hydrophobicity and size of residue, suggesting that the hydrophobic character of this residue is important for catalysis. The Trp 209 was forecast as an important residue and was exposed to computational mutagenesis. This forecast …
Production And Characterization Of Polyclonal Antibodies To Human Recombinant Domain B-Free Antihemophilic Factor Viii, Artem Tykhomyrov, Victor Nedzvetsky, Sergiy Shemet, Can Ali̇ Ağca
Production And Characterization Of Polyclonal Antibodies To Human Recombinant Domain B-Free Antihemophilic Factor Viii, Artem Tykhomyrov, Victor Nedzvetsky, Sergiy Shemet, Can Ali̇ Ağca
Turkish Journal of Biology
Moroctocog alpha is a human B-domain deleted recombinant factor VIII (BDDrFVIII), which represents a new generation of pure antihemophilic products. We describe here an optimized procedure for polyclonal anti-FVIII-antibody production with the use of BDDrFVIII as an immunogen. The main immunochemical characteristics of the produced antibodies and their potential biomedical applications are also reported. Rabbits were immunized with BDDrFVIII as an emulsion with Freund's adjuvant or with antigen immobilized in polyacrylamide gel (PAAG). Antibody titers in immune sera were assayed by enzyme-linked immunosorbent assay (ELISA). IgG purification was performed by affine chromatography on protein A-sepharose. Immune sera and IgG were …
Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural
Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural
Turkish Journal of Biology
In the era of tyrosine kinase inhibitors, resistance still constitutes a problem in chronic myeloid leukemia (CML) patients; thus, new pathway-specific inhibitors like miRNAs have become important in the treatment of refractory patients. There are no satisfying data regarding the miRNAs and anti-miRNA treatment targeting STAT5A and 5B. In this study, we first researched the effect of dasatinib on apoptosis in the CML cell line K562. The expressions of miRNAs possibly targeting both STAT5A and 5B were then determined. The down- and upregulation of the miRNAs were compared using the ΔΔCT method. At the last stage of the study, we …
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Turkish Journal of Biology
For long-term treatment of diabetes type 1, transplantation of insulin-producing beta cells may be a promising method, but the limited number of islets for transplantation requires the development of different approaches. In this study, we aimed to generate beta-like insulin-producing cells. For this purpose, MafA, Pax4, and Ngn3 genes were transferred into pancreatic islet-derived mesenchymal stem cells, and the effect of their ectopic expressions on differentiation efficiency was examined. Stemness properties of pancreatic islet stem cells were characterized. The 3 genes were transfected by electroporation and expressed constitutively. The transfected cells were further stimulated to differentiate by using chemical induction. …