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In Vivo Characterization Of The Drosophila Mrna 3’-End Processing Core Cleavage Complex: Poly(A) Mrna & Histone Mrna 3’-End Processing, Daniel Michalski
In Vivo Characterization Of The Drosophila Mrna 3’-End Processing Core Cleavage Complex: Poly(A) Mrna & Histone Mrna 3’-End Processing, Daniel Michalski
Dissertations
A core cleavage complex (CCC) consisting of CPSF73, CPSF100 and Symplekin is required for co-transcriptional 3’ end processing of all metazoan pre-mRNAs, yet little is known about the in vivo molecular interactions within this complex. The CCC is a component of two distinct complexes, the cleavage/polyadenylation complex and the complex that processes non-polyadenylated histone pre-mRNAs. RNAi-depletion of CCC factors in Drosophila culture cells causes reduction of CCC processing activity on histone mRNAs, resulting in read through transcription. In contrast, RNAi-depletion of factors only required for histone mRNA processing allows use of downstream cryptic polyadenylation signals to produce polyadenylated histone mRNAs. …