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Full-Text Articles in Life Sciences
Atp Luminescence Assay As A Bioburden Estimator Of Biomass Accumulation In Caves, Janez Mulec, Andreea Oarga-Mulec
Atp Luminescence Assay As A Bioburden Estimator Of Biomass Accumulation In Caves, Janez Mulec, Andreea Oarga-Mulec
International Journal of Speleology
A commercially available adenosine triphosphate (ATP) detection system (Hygiena, USA), supported by cultivable microbial indicators, was used to estimate bioburden in different habitats in and outside show caves: air, water and solid surfaces. A strong positive correlation between ATP concentration expressed as Relative Light Units (RLU) and Colony-Forming-Units (CFU) was observed for swab samples from cave surfaces. In terms of ATP units, surfaces in a single cave system (Postojna Cave) varied considerably (240-1,258,800 RLU/ 20 cm2) and commonly exceeded the bioburden level of analogues on the surface (0-114,390 RLU/ 20 cm2). Cave sub-habitats were colonized by …
A Study On The Dissolution Of Autunite Minerals By Facultative Bacteria In Bicarbonate Media, Sandra C. Herrera Landaez
A Study On The Dissolution Of Autunite Minerals By Facultative Bacteria In Bicarbonate Media, Sandra C. Herrera Landaez
FIU Electronic Theses and Dissertations
Uranium (U) is a key contaminant at the Hanford site. The formation of uranyl-phosphate bearing minerals such as autunite as a result of tripoliphosphate injections has been used as a U immobilization strategy. Bacteria are known as key factors governing the fate and transport of soil contaminants. This research evaluated the interaction of facultative bacteria Shewanella Oneidensis MR-1 with autunite mineral in bicarbonate-amended media solutions. The concentration of several elements such as U, calcium (Ca) and phosphorous (P) released as a result of autunite mineral biodissolution were determined as a function of time; changes in cell density and protein assay …