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Full-Text Articles in Life Sciences
Effects Of Macromolecular Crowding On Ferredoxin And Ferredoxin-Nadp+ Reductase Kinetics, Danielle Bautista, David W. Seybert Ph.D.
Effects Of Macromolecular Crowding On Ferredoxin And Ferredoxin-Nadp+ Reductase Kinetics, Danielle Bautista, David W. Seybert Ph.D.
Undergraduate Research and Scholarship Symposium
Macromolecular crowding refers to the intrinsic occurrence of high concentrations of macromolecules found within cells, which may alter biomolecular properties of other macromolecules. Simulating these environments in vitro should enable a more accurate understanding of these modifications. We have employed in vitro studies to specifically examine the effects of macromolecular crowding on biological redox reactions. The iron-sulfur protein ferredoxin (Fdx) and the FAD-containing flavoprotein ferredoxin-NADP+ reductase (FNR), from Spinacea oleracea, were utilized as a model electron transfer system. Utilizing NADPH as a reductant, the Fdx-FNR complex reduces mammalian cytochrome c as a surrogate electron acceptor. Reduction of the heme moiety …
Modeling Chronic Bladder Pain In Male And Female Mice: Exploring The Chronicity Of Repeated Cyclophosphamide Injections., Abigail Cox, Katelyn Sadler, Benedict Kolber
Modeling Chronic Bladder Pain In Male And Female Mice: Exploring The Chronicity Of Repeated Cyclophosphamide Injections., Abigail Cox, Katelyn Sadler, Benedict Kolber
Undergraduate Research and Scholarship Symposium
Millions of people in the United States are afflicted with chronic bladder pain syndromes including interstitial cystitis/bladder pain syndrome (IC/BPS) and chronic prostatitis and chronic pelvic pain syndrome (CP/CPPS). IC/BPS and CP/CPPS patients suffer from generalized pelvic pain, frequent urination with a decrease in urine volume, a constant urge to urinate, and psychological comorbidities in the absence of infection. The etiology is undetermined and treatments are limited. To better understand the mechanisms of IC/BPS and CP/CPPS, many animal models have been developed. In one such model, rodents are injected with cyclophosphamide (CYP), a chemotherapeutic drug that causes cystitis. We are …