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Articles 1 - 30 of 49
Full-Text Articles in Life Sciences
The Subject Librarian Newsletter, Biology, Fall 2016, Sandy Avila
The Subject Librarian Newsletter, Biology, Fall 2016, Sandy Avila
Libraries' Newsletters
No abstract provided.
Making Scholarly Activity Available To The Masses: The Scaffolding Of Scholarship Throughout The Undergraduate Curriculum, Michael Savarese, Trent R. Brown, Carolyn Culbertson, Anna Carlin
Making Scholarly Activity Available To The Masses: The Scaffolding Of Scholarship Throughout The Undergraduate Curriculum, Michael Savarese, Trent R. Brown, Carolyn Culbertson, Anna Carlin
Florida Statewide Symposium: Best Practices in Undergraduate Research
Florida Gulf Coast University’s Quality Enhancement Plan (QEP) focuses on improving student critical thinking, information literacy, and written communication. Rather than developing these skills through traditional methods (e.g., through senior-level, independent research), these learning outcomes are practiced through scholarly experiences. Traditional undergraduate scholarship manifests itself through terminal, senior capstone or research experiences. These, because of the economy of scale, typically reach a minority of students, often just honors students or those approached by faculty mentors. At FGCU, however, scholarly experiences are a part of the curriculum throughout the program of study, and scaffolded to build greater depth and sophistication. Presented …
Strengthening Students’ Information Literacy Skills As They Develop Original Research Proposals In A Scientific Process Course, Kimberly A. Reycraft, Nora E. Demers
Strengthening Students’ Information Literacy Skills As They Develop Original Research Proposals In A Scientific Process Course, Kimberly A. Reycraft, Nora E. Demers
Florida Statewide Symposium: Best Practices in Undergraduate Research
Scientific Process is a required course for all undergraduate science majors at FGCU. In this course, students develop original research proposals on topics of their interest. Information literacy skills are critical as students must be able to use multiple sources of information to develop their proposals. Biology and library faculty have collaborated to add instruction and assignments addressing research question development, search strategy, citation management, and more. Our goal is to improve students’ information literacy skills as well as the quality and quantity of citations in their final proposals. We will present on this initiative and our preliminary assessment results.
The Subject Librarian Newsletter, Interdisciplinary Studies, Fall 2016, Corinne Bishop
The Subject Librarian Newsletter, Interdisciplinary Studies, Fall 2016, Corinne Bishop
Libraries' Newsletters
No abstract provided.
We All Should Strive To Be The Palm In The Storm, Carolyn Massiah
We All Should Strive To Be The Palm In The Storm, Carolyn Massiah
UCF Forum
Hurricane season is beginning to hit its peak activity. Multiple storms have been brewing in the Atlantic the past couple weeks.
How Did We Wind Up In Such An Unlikely Universe?, Michael Bass
How Did We Wind Up In Such An Unlikely Universe?, Michael Bass
UCF Forum
Not long ago the Public Broadcasting Service program NOVA presented an episode called “The Great Math Mystery.” It dealt with the many ways mathematical relationships and special numbers crop up when investigating the physical and natural world.
The Subject Librarian Newsletter, Biology, Spring 2016, Patti Mccall
The Subject Librarian Newsletter, Biology, Spring 2016, Patti Mccall
Libraries' Newsletters
No abstract provided.
Resistance Exercise Elicits Selective Mobilization And Adhesion Characteristics Of Granulocytes And Monocyte Subsets, Adam Jajtner
Resistance Exercise Elicits Selective Mobilization And Adhesion Characteristics Of Granulocytes And Monocyte Subsets, Adam Jajtner
Electronic Theses and Dissertations
Resistance exercise eliciting muscle damage results in an immune response, leading to increases in circulating cytokines, and immune cell mobilization. Classical monocytes respond to muscle damage, however, little is known about the intermediate or nonclassical monocyte response to resistance exercise. Moreover, the impact of polyphenol supplementation in conjunction with resistance exercise on the innate immune response is unknown. The purpose of this study was to examine the immune response following resistance exercise with (PPB) and without (PL) polyphenol supplementation. Thirty-nine untrained men were randomized into three groups: PPB (n=13, 21.8±2.5yrs, 171.2±5.5cm, 71.2±8.2kg), PL (n=15, 21.6±2.5yrs, 176.5±4.9cm, 84.0±15.7kg) or a control …
Habitat Distribution And Abundance Of Crayfishes In Two Florida Spring-Fed Rivers, Tiffani Manteuffel
Habitat Distribution And Abundance Of Crayfishes In Two Florida Spring-Fed Rivers, Tiffani Manteuffel
Electronic Theses and Dissertations
Crayfish are an economically and ecologically important invertebrate, however, research on crayfish in native habitats is patchy at best, including in Florida, even though the Southeastern U.S. is one of the most speciose areas globally. This study investigated patterns of abundance and habitat distribution of two crayfishes (Procambarus paeninsulanus and P. fallax) in two Florida spring-fed rivers (Wakulla River and Silver River, respectively). Study sites were surveyed once each season from April 2015 to March 2016 with baited minnow traps checked every other day, four times each survey. Habitat and environmental parameters evaluated included dominant vegetation or bottom type, percent …
The Role Of Type-I Interferon In Limiting Spread And Killing Of An Oncolytic Rna Virus In Prostate Cells, Kritika Kedarinath
The Role Of Type-I Interferon In Limiting Spread And Killing Of An Oncolytic Rna Virus In Prostate Cells, Kritika Kedarinath
Electronic Theses and Dissertations
Prostate cancer is the second most prevalent cancer amongst men and there is an urgent need to address viable therapeutic options for its treatment. Development of viruses which target and kill cancer cells has gained momentum due to the first FDA approved oncolytic virus for treating human cancer patients. Our previous work with the RNA virus, Parainfluenza Virus 5 (PIV5), has led to the generation of mutants that are potential candidates for oncolytic viruses: 1) the hyperfusogenic (P/V/F) mutant has a mutated P/V and fusion gene which activates anti-viral responses and causes massive cell-cell fusion respectively, and 2) the Leader …
Bmp-7 Inhibits P38 And Jnk Pathways And Increases M2 Macrophage Differentiation To Reduce Atherosclerosis In Apolipoprotein E-/- Mice, Heidi Shoulders
Bmp-7 Inhibits P38 And Jnk Pathways And Increases M2 Macrophage Differentiation To Reduce Atherosclerosis In Apolipoprotein E-/- Mice, Heidi Shoulders
Electronic Theses and Dissertations
We have previously shown that treating atherosclerosis with bone morphogenetic protein-7 (BMP-7) affects the presence of macrophage subtypes in vitro, however it remains unknown whether BMP-7 treatment affects development and progression of atherosclerosis in vivo at an early and mid-stage of the disease. We therefore performed a Day 5 (D5) and Day 28 (D28) study to examine BMP-7's potential to affect monocyte differentiation. Atherosclerotic plaque formation was developed using our standard method and ApoE-/- mice were sacrificed at D5 and D28 post-surgery. Treatment animals received intravenous injections of BMP-7 at 200µg/kg of bodyweight. Hematoxylin and Eosin morphological stain shows that …
The Development Of Motuporamine Derivatives And An Investigation Into Their Biological Properties, Kristen Skruber
The Development Of Motuporamine Derivatives And An Investigation Into Their Biological Properties, Kristen Skruber
Electronic Theses and Dissertations
This project investigates the synthesis of a class of compounds derived from a marine-based natural product and probes how iterative changes to its structure affect its derivatives' biological efficacy. The compound class of interest are the motuporamines which were isolated from the sea sponge Xestospongia exigua collected off the coast of Motupore island in Papua, New Guinea. The compounds for this project are predicated upon dihydromotuporamine C (Motu33), the compound that has been shown to be both cytotoxic to MDA-MB231 breast carcinoma cells and has antimetastatic efficacy. The motuporamine scaffold contains a large fifteen-membered saturated macrocycle and an appended polyamine …
Pyroglutamylated Amyloid Beta Peptides Enhance Non-Fibrillogenic Aggregation Of The Unmodified Peptide, Gregory Goldblatt
Pyroglutamylated Amyloid Beta Peptides Enhance Non-Fibrillogenic Aggregation Of The Unmodified Peptide, Gregory Goldblatt
Electronic Theses and Dissertations
Alzheimer's disease (AD) is accompanied by abnormal extracellular deposition of amyloid b (Ab) peptide. This has led to the amyloid cascade hypothesis, causatively relating Ab with AD. While Ab deposits assume a fibrillar cross-b structure, prefibrillar oligomers of Ab have been identified as the main cytotoxic agents in AD. Pyroglutamylated amyloid beta (AbpE) peptides are N-terminally truncated and pyroglutamylated (at Glu3 or Glu11) Ab molecules that display enhanced cytotoxicity and represent up to 50% of total Ab in AD brains. AbpE significantly enhances the toxicity of unmodified Ab by an unknown mechanism. Although in situ Ab populations are heterogeneous, the …
Chlamydia Trachomatis Transformants Show A Significant Reduction In Rates Of Invasion Upon Removal Of Key Tarp Domains, Christopher Parrett
Chlamydia Trachomatis Transformants Show A Significant Reduction In Rates Of Invasion Upon Removal Of Key Tarp Domains, Christopher Parrett
Electronic Theses and Dissertations
Chlamydia trachomatis is an obligate, intracellular bacterium which is known to cause multiple human infections including nongonococcal urethritis (serovars D-K), lymphogranuloma venereum (serovars L1, L2, L3) and trachoma (serovars A-C). The infectious form of the bacterium, called the elementary body (EB), harbors a type III secreted effector known as Tarp (translocated actin recruiting phosphoprotein) which is a candidate virulence factor and is hypothesized to play a role in C. trachomatis' ability to invade and grow within epithelial cells in a human host. C. trachomatis L2 Tarp harbors five unique protein domains which include the Phosphorylation Domain, the Proline Rich Domain, …
Mathematical Modeling Of Heart Rate Deflection Point In Relation To Respiratory Compensation And Treadmill Running Performance, Kayla Baker
Electronic Theses and Dissertations
Heart rate deflection point (HRDP), identified as the second breakpoint in the "intensity / heart rate" relationship, is indicative of the anaerobic threshold (AT). This point can be determined via bi-segmental linear regression (2SEG) or through use of the maximal distance model (D-max); however, the relationship between these methods has yet to be investigated 2. Purpose: To compare the use of 2SEG and D-max methods to determine HRDP and to examine the relationship between these values with a metabolic threshold, respiratory compensation point (RCP), as well as running performance [5,000 meter treadmill time trial (5Ktime)]. Methods: Nineteen recreationally active men …
The Reproducibility And Validity Of Using The Dmax Method To Predict Physical Working Capacity At Fatigue Threshold, Joshua Riffe
The Reproducibility And Validity Of Using The Dmax Method To Predict Physical Working Capacity At Fatigue Threshold, Joshua Riffe
Electronic Theses and Dissertations
Although the original (ORG) physical working capacity at fatigue threshold (PWCFT) assessment has demonstrated strong reliability and sensitivity to training and/or nutritional interventions, limitations may exist regarding the method of PWCFT estimation. However, a relatively new mathematical method, called Dmax, has been shown to be objective and reliable when estimating metabolic and neuromuscular fatigue thresholds. To date, however, no study has examined whether the Dmax method for estimating the work rate at PWCFT is similar to the ORG method. PURPOSE: The purpose of this study is to evaluate the reliability and validity of the Dmax-EMG method for estimating and tracking …
Evaluation Of Echogenicity Within And Between Ultrasonographic Images Of The Vastus Lateralis, Alyssa Varanoske
Evaluation Of Echogenicity Within And Between Ultrasonographic Images Of The Vastus Lateralis, Alyssa Varanoske
Electronic Theses and Dissertations
PURPOSE: The purpose of this study was to examine the echo intensity (EI) values of different ultrasound (US) images of the vastus lateralis (VL) using panoramic imaging in the transverse plane (PTI) and still imaging in the longitudinal plane (SLI). Secondary purposes of this study were to examine VL homogeneity and to determine relationships between subcutaneous adipose tissue thickness (SubQ) adjacent to the muscle and EI. METHODS: Twenty-four recreationally trained collegiate males (20.2 ± 1.6 years; height: 178.1 ± 6.6 cm; weight: 82.2 ± 13.4 kg) participated in this investigation. EI, cross-sectional area (CSA), muscle thickness (MT), and SubQ of …
Intramuscular Tnf-Alpha Signaling In Response To Resistance Exercise And Recovery In Untrained Males., Jeremy Townsend
Intramuscular Tnf-Alpha Signaling In Response To Resistance Exercise And Recovery In Untrained Males., Jeremy Townsend
Electronic Theses and Dissertations
INTRODUCTION: The nuclear factor kappa B (NF-κβ) signaling cascade is responsible for mediating stress-activated catabolic effects of Tumor Necrosis Factor Alpha (TNF-α) downstream of the TNF receptor (TNFR1). NF-κβ transcription factor remains inhibited in the cytosol of the muscle and can be stimulated for translocation and transcription by a variety of external stimuli, most notably by pro-inflammatory cytokines and oxidative stress. Nevertheless, currently there is a gap in the literature with regard to the time course effect of NF-κβ signaling following acute resistance exercise in humans. PURPOSE: To observe the effects of an acute lower-body resistance exercise protocol and subsequent …
Assessment Of Staphylococcus Aureus Genetics: Clinical Versus Community Epidemiology, Matthew Lawrance
Assessment Of Staphylococcus Aureus Genetics: Clinical Versus Community Epidemiology, Matthew Lawrance
Electronic Theses and Dissertations
Staphylococcus aureus has an historical relationship with anthropogenic environments, particularly hospitals, where infection characteristics differ from community-acquired disease. This has promoted a designation of strains as healthcare or community associated. Despite this affiliation, genetic approaches have failed to support these groupings. In order to establish the genetic relationship between S. aureus from differing anthropogenic environments, I have analyzed the relatedness between three cohorts of S. aureus: nasal carriage isolates from community participants, infectious isolates from hospitals, and a cohort from an uninvestigated environment, an ambulatory clinic. Multilocus Sequence Typing (MLST) and Staphylococcus aureus protein a (spa) repeat regions were analyzed …
Neurological Profile Of Older Apoe-Pon1 Double Knockout Mice, Connie Mitra
Neurological Profile Of Older Apoe-Pon1 Double Knockout Mice, Connie Mitra
Electronic Theses and Dissertations
Atherosclerosis is a cardiovascular disease where plaques made up of lipids in the form of cholesterol ester build up in the carotid and innominate arteries that supply blood to the brain. Accumulation of the plaques limit the flow of blood and nutrients to the brain, leading to diminished oxygen supply, increased oxidative stress and cell death. All these have been implicated in Alzheimer's disease (AD). Alzheimer's disease, a chronic, progressive, age related neurodegenerative disorder is the most common form of dementia in the elderly accounting for 60-80% of the cases. Clinically, Alzheimer's disease is characterized by loss of memory, damage …
Transforming The Aquatic Urban Landscape: Nutrient Status And Management Of Stormwater Basins, Lindsay Skovira
Transforming The Aquatic Urban Landscape: Nutrient Status And Management Of Stormwater Basins, Lindsay Skovira
Electronic Theses and Dissertations
Urbanization is a largely irreversible anthropogenic change that degrades environmental quality, including aquatic ecosystems. Stormwater ponds are a popular best management practice (BMP) to mitigate the effects of urban land use on downstream water bodies and contribute significantly to the total area of aquatic ecosystems in some urban watersheds. My research investigated the distribution of stormwater ponds and examined how different urban land uses influenced biophysicochemical conditions and management of those ponds in a rapidly developing suburban watershed in the Econlockhatchee River basin in Florida, USA. I evaluated limnological and ecological parameters in randomly-selected ponds distributed among three urban land-use …
Apolipoprotein-Ai Regulates Hepatic Vldl Secretion By Controlling Intracellular Vldl-Trafficking, Bhavesh Gurwani
Apolipoprotein-Ai Regulates Hepatic Vldl Secretion By Controlling Intracellular Vldl-Trafficking, Bhavesh Gurwani
Electronic Theses and Dissertations
Cardiovascular diseases cause 17 million deaths annually, which is estimated to increase to 23 million deaths by the year 2030. One of the major risk factors for the pathogenesis of cardiovascular diseases is increased secretion of very-low density lipoproteins (VLDL) by the liver; however, reduced VLDL-secretion causes fatty liver disease. Synthesis and secretion of VLDL by the liver plays an important role in maintaining overall lipoprotein homeostasis. Assembly of VLDL occurs along with the expression of apolipoproteinB-100 (apoB100) and its lipidation at the endoplasmic reticulum (ER) level. Once formed in the ER lumen, the nascent VLDL is transported to the …
Role Of Kruppel-Like Factor 8 (Klf8) In Cancer And Cardiomyopathy, Satadru Lahiri
Role Of Kruppel-Like Factor 8 (Klf8) In Cancer And Cardiomyopathy, Satadru Lahiri
Electronic Theses and Dissertations
Cancer and cardiovascular diseases are two most fatal diseases causing innumerable death each year. Understanding the mechanisms underlying these diseases is critical for developing proper therapeutic approach. Kruppel-like factor 8 (KLF8) is a member of Kruppel-like family transcription factors that is overexpressed in many types of cancers. There is no report on role of KLF8 in cardiovascular diseases to date. KLF8 transcriptionally activates or represses a host of target genes to promote cancer cell proliferation, migration, invasion and epithelial to mesenchymal transition during tumor progression. Studies proposed in this thesis identified a novel posttranslational modification of KLF8 essential for its …
Vo-Ohpic Treatment Reduces Cardiac Remodeling In Doxorubicin-Induced Cardiomyopathy, Taylor Johnson
Vo-Ohpic Treatment Reduces Cardiac Remodeling In Doxorubicin-Induced Cardiomyopathy, Taylor Johnson
Electronic Theses and Dissertations
Doxorubicin (Doxo) is one of multiple anthracycline drugs used to effectively treat various forms of cancer. Unfortunately, Doxo treatment, as a side effect, induces cardiomyopathy and subsequent heart failure. We have previously demonstrated that transplanted embryonic stem (ES) cells and their conditioned medium (CM) modulate the PTEN pathway and reduce apoptosis, fibrosis and hypertrophy in a Doxo induced cardiomyopathy (DIC) model. However, mechanisms of inhibited apoptosis mediated through PTEN pathway are completely unknown. Therefore, we used VO-OHpic (VO), a potent PTEN inhibitor to understand the mechanism of apoptosis as well as its effect on cardiac remodeling in DIC. Animals were …
Malondialdehyde (Mda) And Glutathione Peroxidase (Gpx) Are Elevated In Crohns Disease-Associated With Mycobacterium Avium Subspecies Paratuberculosis (Map), Ahmad Qasem
Electronic Theses and Dissertations
Inflamed tissue in Crohn’s disease (CD) are continuously producing toxic oxygen metabolites leading to cellular injury and apoptosis. Here, we are evaluating the role of Mycobacterium avium subspecies paratuberculosis (MAP) in oxidative stress in CD by evaluation of lipid peroxidation and antioxidant defense activity. Specifically, we measured malondialdehyde (MDA) level and selenium-dependent glutathione peroxidase (GPx) activity in the plasma from patients and cattle infected with MAP. The level of MAP antibodies in bovine sera was determined by IDEXX kit whereas detection of MAP DNA was performed by IS900-based nPCR. A total of 42 cattle (21 infected with MAP and 21 …
Involvement Of Mirnas In The Development Of Androgen Independent Prostate Cancer, Richard Ottman
Involvement Of Mirnas In The Development Of Androgen Independent Prostate Cancer, Richard Ottman
Electronic Theses and Dissertations
Development of resistance to androgen deprivation therapy (ADT) is a major obstacle for the management of advanced prostate cancer. Therapies with androgen receptor (AR) antagonists and androgen withdrawal initially result in tumor regression but development of compensatory mechanisms including AR bypass signaling leads to tumor re-growth, independent of circulating androgens. The result is the emergence of castration resistant prostate cancer (CRPC), a highly morbid disease exhibiting aberrant expression of many protein-coding and non-coding genes. Under the umbrella of non-coding RNAs is a class of small regulatory RNAs referred to as microRNAs (miRNAs). MicroRNAs are believed to function in the maintenance …
Flying Under The Lidar: Relating Forest Structure To Bat Community Diversity, Anna Schneider Swanson
Flying Under The Lidar: Relating Forest Structure To Bat Community Diversity, Anna Schneider Swanson
Electronic Theses and Dissertations
Bats are important to many ecological processes such as pollination, insect (and by proxy, disease) control, and seed dispersal and can be used to monitor ecosystem health. However, they are facing unprecedented extinction risks from habitat degradation as well as pressures from pathogens (e.g., white-nose syndrome) and wind turbines. LiDAR allows ecologists to measure structural variables of forested landscapes with increased precision and accuracy at broader spatial scales than previously possible. This study used airborne LiDAR to classify forest habitat/canopy structure at the Ordway-Swisher Biological Station (OSBS) in north central Florida. LiDAR data were acquired by the National Ecological Observatory …
Pasture Management, The Red Imported Fire Ant (Solenopsis Invicta) And Dung Beetle Mediated Ecosystem Services, Christen Steele
Pasture Management, The Red Imported Fire Ant (Solenopsis Invicta) And Dung Beetle Mediated Ecosystem Services, Christen Steele
Electronic Theses and Dissertations
Grazing animals in Florida deposit over 20 million metric tons of dung per year, making dung a significant non-point source of pollution (extrapolated from Fincher, 1981). Degradation of this dung occurs naturally, primarily due to a diverse group of beetles (Order Coleoptera) primarily in the families Scarabaeidae and Geotrupidae(hereafter dung beetles). Dung that is not degraded becomes a non-point source of pollutants and may be leached into water bodies. Additionally, dung provides an incubation site for the pests and parasites of both humans and livestock. Thus, as dung beetles consume and degrade dung, they provide a multitude of ecosystem services …
From Pet To Pest? The Potential Global Range And Food Web Effects Of A Generalist Carnivore, Hannah Bevan
From Pet To Pest? The Potential Global Range And Food Web Effects Of A Generalist Carnivore, Hannah Bevan
Electronic Theses and Dissertations
The Nile monitor lizard [Varanus niloticus (Linnaeus, 1766)] is a generalist carnivore, native to Sub-Saharan Africa and the Nile River but now established in North America as a result of the pet trade. Once introduced, they are a potential invasive threat to native wildlife. Here, I create ensemble species distribution models (SDMs) to predict the global distribution of this generalist carnivore given current and future climate conditions. I then quantify the monitor's potential effects on 85 food webs representing >900 different species within the projected regions based on stomach content data. Climate, vegetation, and elevation data are used for 507 …
Genetic Differentiation Among Florida Populations Of Diadema Antillarum, Luke M. Chandler
Genetic Differentiation Among Florida Populations Of Diadema Antillarum, Luke M. Chandler
Honors Undergraduate Theses
This project used molecular genetic markers (microsatellites) to determine the amount of genetic diversity within populations and whether significant differentiation exists among Florida populations of the long-spined sea urchin, Diadema antillarum. Specifically, this project aimed to (1) compare genetic diversity of D. antillarum from six populations in south Florida ranging from Biscayne Bay, the Florida Keys, and Dry Tortugas, and (2) determine whether two broodstock populations of D. antillarum contain variation indicative of native Florida populations. Together, these questions can address whether broodstock populations contain the genetic variation necessary to meet the Florida Fish and Wildlife Conservation Commission’s (FWC’s) genetic …