Life Sciences Commons^™

Elevated Co2 Induced Changes In The Chemistry Of Quaking Aspen (Populus Tremuloides Michaux) Leaf Litter: Subsequent Mass Loss And Microbial Response In A Stream Ecosystem, Steven Rier, Nancy Tuchman, Robert Wetzel, James Teeri

Nancy Tuchman

No abstract provided.

Engineering, Nutrient Removal, And Feedstock Conversion Evaluations Of Four Corn Stover Harvest Scenarios, Reed L. Hoskinson, Douglas L. Karlen, Stuart J. Birrell, Corey W. Radtke, W. W. Wilhelm

Douglas L Karlen

Crop residue has been identified as a near-term source of biomass for renewable fuel, heat, power, chemicals and other bio-materials. A prototype one-pass harvest system was used to collect residue samples from a corn (Zea mays L.) field near Ames, IA. Four harvest scenarios (low cut, high-cut top, high-cut bottom, and normal cut) were evaluated and are expressed as collected stover harvest indices (CSHI). High-cut top and high-cut bottom samples were obtained from the same plot in separate operations. Chemical composition, dilute acid pretreatment response, ethanol conversion yield and efficiency, and thermochemical conversion for each scenario were determined. Mean grain …

Connectivity Of Coastal And Oceanic Ecosystems: Pelagic Habitat Use By Juvenile Reef Fishes In The Gulf Of Mexico, Katie Bowen

HCNSO Student Theses and Dissertations

The assemblage structure, abundance, biomass, and vertical distribution of juvenile reef fishes in the offshore pelagic habitat of the northern Gulf of Mexico are described as part of the NOAA-supported Offshore Nekton Sampling and Analysis Program. The results presented here are from a 3-month, continuous sampling series in 2011 in which discrete depth strata from 0 to 1500 m were sampled using a 10-m² MOCNESS midwater trawl. This is the first study to examine pelagic juvenile reef fish distributions across the entire oceanic northern Gulf of Mexico seaward of the continental shelf break after the Deepwater Horizon Oil Spill. …

Estimating Herbaceous Biomass Of Grassland Vegetation Using The Reference Unit Method, Eric D. Boyda, Jack L. Bulter, Lan Xu

The Prairie Naturalist

Aboveground net primary production provides valuable information on wildlife habitat, fire fuel loads, and forage availability. Aboveground net primary production in herbaceous plant communities is typically measured by clipping aboveground biomass. However, the high costs associated with physically harvesting plant biomass may prevent collecting sufficient data to account for natural spatial and temporal variability of vegetation at a landscape scale. Various double-sampling techniques have been developed to increase sample size while reducing cost. We applied a biomass estimation technique previously developed for estimating shrub biomass using representative samples or “reference units” to estimate herbaceous grassland biomass. Our reference units consisted …

Production And Harvest Of Microalgae In Wastewater Raceways With Resource Recycling, Alexander Colin Roberts

Master's Theses

Microalgae can be grown on municipal wastewater media to both treat the wastewater and produce feedstock for algae biofuel production. However the reliability of treatment must be demonstrated, as well as high areal algae productivity on recycled wastewater media and efficient sedimentation harvesting. This processes was studied at pilot scale in the present research.

A pilot facility was operated with nine CO₂-supplemented raceway ponds, each with a 33-m² surface area and a 0.3-m depth, continuously from March 6, 2013 through September 24, 2014. The ponds were operated as three sets of triplicates with two sets continuously fed …

Alternative Strategies In Response To Saline Stress In Two Varieties Of Portulaca Oleracea (Purslane), Dana A. Dudle, Kristina R. Mulry, Bryan A. Hanson

Biology Faculty publications

Purslane (Portulaca oleracea) is a globally-distributed plant with a long history of use in folk medicine and cooking. We have developed purslane as a model system for exploring plant responses to stress. We exposed two varieties of purslane to saline stress with the objective of identifying differences between the varieties in the plasticity of morphological and physiological traits. The varieties responded to saline stress with significantly different changes in the measured traits, which included inter alia biomass, flower counts, proline concentrations and betalain pigment concentrations. The alternative responses of the two varieties consisted of complex, simultaneous changes in …

Can The Results Of Biodiversity-Ecosystem Productivity Studies Be Translated To Bioenergy Production?, Timothy L. Dickson, Katherine L. Gross

Biology Faculty Publications

Biodiversity experiments show that increases in plant diversity can lead to greater biomass production, and some researchers suggest that high diversity plantings should be used for bioenergy production. However, many methods used in past biodiversity experiments are impractical for bioenergy plantings. For example, biodiversity experiments often use intensive management such as hand weeding to maintain low diversity plantings and exclude unplanted species, but this would not be done for bioenergy plantings. Also, biodiversity experiments generally use high seeding densities that would be too expensive for bioenergy plantings. Here we report the effects of biodiversity on biomass production from two studies …

Prospecting For Energy-Rich Renewable Raw Materials: Agave Leaf Case Study, Kendall R. Corbin, Caitlin S. Byrt, Stefan Bauer, Seth Debolt, Don Chambers, Joseph A. M. Holtum, Ghazwan Karem, Marilyn Henderson, Jelle Lahnstein, Cherie T. Beahan, Antony Bacic, Geoffrey B. Fincher, Natalie S. Betts, Rachel A. Burton

Horticulture Faculty Publications

Plant biomass from different species is heterogeneous, and this diversity in composition can be mined to identify materials of value to fuel and chemical industries. Agave produces high yields of energy-rich biomass, and the sugar-rich stem tissue has traditionally been used to make alcoholic beverages. Here, the compositions of Agave americana and Agave tequilana leaves are determined, particularly in the context of bioethanol production. Agave leaf cell wall polysaccharide content was characterized by linkage analysis, non-cellulosic polysaccharides such as pectins were observed by immuno-microscopy, and leaf juice composition was determined by liquid chromatography. Agave leaves are fruit-like--rich in moisture, …

Cover Crop Biomass Production And Water Use In The Central Great Plains, David C. Nielsen, Drew J. Lyon, Gary W. Hergert, Robert K. Higgins, Johnathon D. Holman

David C. Nielsen

The water-limited environment of the semiarid Central Great Plains may not produce enough cover crop biomass to generate benefits associated with cover crop use in more humid regions. There have been reports that cover crops grown in mixtures produce more biomass with greater water use efficiency than single-species plantings. This study was conducted to determine differences in cover crop biomass production, water use efficiency, and residue cover between a mixture and single-species plantings. The study was conducted at Akron, CO, and Sidney, NE, during the 2012 and 2013 growing seasons under both rainfed and irrigated conditions. Water use, biomass, and …

Evaluation Of Two Non-Destructive Methods For Estimating Biomass In A Prairie, Arnoldas Pivorius, Laura Ploughe, Jeff Dukes

The Summer Undergraduate Research Fellowship (SURF) Symposium

The estimation of above ground biomass is important in managing natural resources, like assessing habitat conditions. Direct, destructive measurements by clipping, drying, and weighing plant matter is time consuming and labor intensive. It is of interest to develop non-invasive methods to complement or replace direct vegetation harvest. Two non-invasive methods were evaluated, light penetration and visual obstruction, in the Purdue Wildlife Area prairie. Light penetration in the grassland plots were measured using a Decagon light stick (LP-80), 5 cm from the ground and above the plants. Estimation of visual obstruction was done using a Robel pole, which focuses on the …

Competence Evaluation Of Mycodiesel Production By Oleaginous Fungal Strains: Mucor Circinelloides And Gliocladium Roseum, Sandip S. Magdum, Gauri P. Minde, Upendra S. Adhyapak, V. Kalyanraman

Sandip S. Magdum

Comparing with lesser algal growth rate for biofuel production along with many constraints, fungal route should be analyzed for its capability of biodiesel or mycodiesel production. The two fungal strains namely, Mucor circinelloides (MTCC1297) and Gliocladium roseum (MTCC6474) were analyzed for laboratory scale mycodiesel production. The M. circinelloides and G. roseum were able to produce biomass of 0.404 mg VSS/mg sucrose and 0.642 mg VSS/ mg sucrose with the mycodiesel content of 20.69% (w/w) and 11.37% (w/w) respectively. Furthermore, qualitative analysis of the oil contents by GC-MS were identified the presence of Tetradecanoic and Octadecanoic acids.

Lidar And Machine Learning Estimation Of Hardwood Forest Biomass In Mountainous And Bottomland Environments, Bowei Xue

Graduate Theses and Dissertations

Light detection and ranging (lidar) has been applied in various forest applications, such as to retrieve forest structural information, to build statistical models for identification of tree species, and to monitor forest growth. However, despite significant progress in these areas, the choice of regression approach and parameter tuning remains an ongoing critical question. This study focused on choosing the right spatial generalization level to transform lidar point clouds to 2D images which can be further processed by mature image processing and pattern recognition approaches. It also compared the prediction ability of popular machine learning algorithms applied to aboveground forest biomass …

Assessing Site Availability Of Aspen And Northern Hardwoods For Potential Feedstock Development In Michigan: A Case Study, Sara Alian, Ann Maclean

Michigan Tech Publications

The importance of wood and wood byproducts as biomass feedstocks is of increasing interest as a source of ethanol and electricity. Second generation woody feedstock sources in Michigan, e.g., hybrid poplar and hybrid willow (Populus spp.), and native forests, particularly aspen and northern hardwoods, are a potential source of woody biomass for these uses. This study provides a geographic information system (GIS) framework for assessing the current spatial extent of aspen and northern hardwoods) and their proximity to roads. Additionally, the potential for expanding the area of these feedstock sources based on pre-European settlement vegetation cover is assessed. Utilizing GIS …

Anaerobic Detoxification Of Acetic Acid In A Thermophilic Ethanologen, A Joe Shaw, Bethany B. Miller, Stephen R. Rogers, William Robert Kenealy, Alex Meola, Ashwini Bhandiwad, W Ryan Sillers, Indraneel Shikhare, David Hogsett, Christopher Herring

Dartmouth Scholarship

The liberation of acetate from hemicellulose negatively impacts fermentations of cellulosic biomass, limiting the concentrations of substrate that can be effectively processed. Solvent-producing bacteria have the capacity to convert acetate to the less toxic product acetone, but to the best of our knowledge, this trait has not been transferred to an organism that produces ethanol at high yield. We have engineered a five-step metabolic pathway to convert acetic acid to acetone in the thermophilic anaerobe Thermoanaerobacterium saccharolyticum.

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Characterization And Fluorescence Of Yellow Biofilms In Karst Caves, Southwest Slovenia, Janez Mulec, Andreea Oarga-Mulec, Rok Tomazin, Tadeja Matos

International Journal of Speleology

Biofilms of different colours that colonize surfaces within karst caves represent a source of nutrients. They occur commonly and abundantly at sites with sediments, and close to seepages or underground rivers. Golden-yellow subaerial biofilms are particularly well observed because of their contrast with their surroundings, the characteristics of the pigment and recently, even more, due to the characteristics of light-emitting diode (LED) illumination. Yellow microbial biofilms were sampled from three caves in southwestern Slovenia, Dimnice, Križna jama and Sveta jama. The highest concentration of cultivable microbes (2.33×10⁸ CFU/g) and the biggest number of identified bacteria (66.0%) were retrieved from …

Enhancing The Sustainability Of Integrated Biofuel Feedstock Production Systems, Amanda Joy Ashworth

Doctoral Dissertations

As use of second-generation biofuel crops increases, so do questions about sustainability, particularly their potential to affect fossil energy consumption and greenhouse gas emissions. Nitrogen (N)-fixing legumes interseeded into switchgrass (Panicum virgatum L.) may be an alternative to inorganic fertilizer in forage-feedstock systems. Research herein is divided into four general experiments: I). N replacement and feedstock impacts from legume intercrops and biochar in switchgrass; II). N-fixation rates in intercrop systems; III). impacts of biofuel systems under enhanced climate change; and, IV). projected sustainability of regional switchgrass production. Approaches included: characterization of feedstock/forage quality traits based on legume, biochar and …

Diallel Analysis Of Biomass And Ethanol Yield In Leaves Versus Stems Of Lowland Switchgrass, Alexandria Christina Desantis

Masters Theses

Switchgrass (Panicum virgatum L.) is a warm season perennial grass used widely as a forage crop. This research seeks to address improving biomass yield and predicted ethanol yield through certain traits by the following objectives: (1) differences in average biomass and predicted ethanol yields from leaves versus stems, (2) genetic variance and heritability estimates of biomass and ethanol yield traits (3) general (GCA) and specific combining ability (SCA) among the five parents for biomass and ethanol yield, and (4) correlations between agronomic and morphological traits. Five parents with varying morphological traits were crossed in a diallel design, excluding selfs. …

Phytoextraction Of Lead From Contaminated Urban Soils By Panicum Virgatum Enhanced With Phytohormone, Edta, Benomyl, And Citric Acid Application, Matthew Aderholt

Master of Science in Integrative Biology Theses

Lead (Pb) contamination in soil represents a threat to human health and cleanup efforts often requires expensive or environmentally disruptive techniques. Phytoextraction has gained attention as a possible alternative to traditional remediation methods because of lower cost and minimal soil disruption. Panicum virgatum L. (switchgrass) was chemically enhanced with applications of the soil-fungicide benomyl, chelates (EDTA and citric acid), and phytohormones for phytoextraction of Pb from contaminated urban soils in Atlanta, GA. EDTA is often regarded as the most effective chelate, but has potential problems associated with its use due to long persistence time in soil and possible Pb mobilization …

Winter Rye As A Bioenergy Feedstock: Impact Of Crop Maturity On Composition, Biological Solubilization And Potential Revenue, Xiongjun Shao, Kay Dimarco, Tom L. Richard, Lee R. Lynd

Dartmouth Scholarship

Background:

Winter annual crops such as winter rye (Secale cereale L) can produce biomass feedstock on seasonally fallow land that continues to provide high-value food and feed from summer annuals such as corn and soybeans. As energy double crops, winter grasses are likely to be harvested while still immature and thus structurally different from the fully senesced plant material typically used for biofuels. This study investigates the dynamic trends in biomass yield, composition, and biological solubilization over the course of a spring harvest season.

Results:

The water soluble fraction decreased with increasing maturity while total carbohydrate content stayed …

Utilizing National Agriculture Imagery Program Data To Estimate Tree Cover And Biomass Of Piñon And Juniper Woodlands, April Hulet, Bruce A. Roundy, Steven L. Petersen, Stephen C. Bunting, Ryan R. Jensen, Darrell B. Roundy

Articles

With the encroachment of piñon (Pinus ssp.) and juniper (Juniperus ssp.) woodlands onto sagebrush steppe rangelands, there is an increasing interest in rapid, accurate, and inexpensive quantification methods to estimate tree canopy cover and aboveground biomass. The objectives of this study were 1) to evaluate the relationship and agreement of piñon and juniper (P-J) canopy cover estimates, using object-based image analysis (OBIA) techniques and National Agriculture Imagery Program (NAIP, 1-m pixel resolution) imagery with ground measurements, and 2) to investigate the relationship between remotely-sensed P-J canopy cover and ground-measured aboveground biomass. For the OBIA, we used eCognition® Developer …

The Bifunctional Alcohol And Aldehyde Dehydrogenase Gene, Adhe, Is Necessary For Ethanol Production In Clostridium Thermocellum And Thermoanaerobacterium Saccharolyticum, Jonathan Lo, Tianyong Zheng, Shuen Hon, Daniel G. Olson, Lee Lynd

Dartmouth Scholarship

Thermoanaerobacterium saccharolyticum and Clostridium thermocellum are anaerobic thermophilic bacteria being investigated for their ability to produce biofuels from plant biomass. The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is present in these bacteria and has been known to be important for ethanol formation in other anaerobic alcohol producers. This study explores the inactivation of the adhE gene in C. thermocellum and T. saccharolyticum. Deletion of adhE reduced ethanol production by >95% in both T. saccharolyticum and C. thermocellum, confirming that adhE is necessary for ethanol formation in both organisms. In both adhE deletion strains, fermentation products shifted from ethanol …

Effects Of Aging On Organic Aerosol From Open Biomass Burning Smoke In Aircraft And Laboratory Studies, M J. Cubison, A M. Ortega, P L. Hayes, D K. Farmer, D Day, M J. Lechner, W H. Brune, E Apel, G S. Diskin, J A. Fisher, H E. Fuelberg, A Hecobian, D J. Knapp, T Mikoviny, D Riemer, G W. Sachse, W Sessions, R Weber, A J. Weinheimer, A Wisthaler, J L. Jimenez

Jenny A Fisher

Biomass burning (BB) is a large source of primary and secondary organic aerosols (POA and SOA). This study addresses the physical and chemical evolution of BB organic aerosols. Firstly, the evolution and lifetime of BB POA and SOA signatures observed with the Aerodyne Aerosol Mass Spectrometer are investigated, focusing on measurements at high-latitudes acquired during the 2008 NASA ARCTAS mission, in comparison to data from other field studies and from laboratory aging experiments. The parameter f60 , the ratio of the integrated signal at m/z 60 to the total signal in the organic component mass spectrum, is used as a …

Characterization Of Two Biochars Derived From Horse Muck And Their Ability To Reduce Pathogen Transport In Soil, David Griffith

Theses and Dissertations--Biosystems and Agricultural Engineering

Biochars have been created and characterized from a variety livestock manure biomass including poultry, dairy, and swine. However, no research has been conducted on the physical and chemical makeup of biochar pyrolyzed from horse muck. Two horse muck derived biochars containing either straw (HS) or woodchip (HW) bedding were pyrolyzed at 700°C and characterized for their physical and chemical properties. Tests revealed both biochars had high alkalinity, moderate specific conductivity, and low surface area as compared to other biochars in the literature. HS contained more mineral structures than HW. Scanning electron microscopy presented differences in particle shape, size, and presence …

Zooplankton Abundance, Biomass, And Size Structure In The Coastal Waters Of The Northeastern Mediterranean Sea, Tuba Kurt, Sevi̇m Polat

Turkish Journal of Zoology

Zooplankton was studied seasonally between 2009 and 2011 in İskenderun Bay in order to determine temporal variations in abundance, biomass, and size structure and their relationships with environmental factors. Zooplankton sampling was performed vertically using a WP-2 net (200-µm). A total of 30 zooplankton taxonomic groups were identified. Cladocera was the dominant group, and together with Copepoda and Appendicularia constituted approximately 90% of zooplankton. In respect to abundance and biomass values, 2 peaks were observed in 2009, the first in spring and the second in autumn. However, a second peak was observed in summer in both 2010 and 2011, due …

Effects Of Gradual Soil Drought Stress On The Growth, Biomass Partitioning, And Chlorophyll Fluorescence Of Prunus Mongolica Seedlings, Youyan Guo, Hongyuan Yu, Dongsheng Kong, Fang Yan, Donghua Liu, Yajuan Zhang

Turkish Journal of Biology

The present studyaimed to determine the effects of gradual drought stress on Prunus mongolica seedlings. The growth and chlorophyll fluorescence of the seedlings were investigated under drought and control conditions. The short-term (21 day) decline in soil water content from 30.46% to 15.55% did not significantly influence the growth of P. mongolica seedlings but increased the height, basal diameter, crown, leaf number, stem mass, leaf mass, and root mass of the seedlings. The growth of the drought group reached the maximum, but remained lower than that of the control group, within 21 days. Compared with the control group, the drought …

Influence Of Culture Media And Carbon Sources On Biomass Productivity And Oil Content Of The Algae Sirogonium Sticticum, Temnogyra Reflexa, Uronema Elongatum, And Chroococcus Turgidus, Aftab Alam, Saleem Ullah, Sahib Alam, Hamid Ullah Shah, Saadia Aftab, Muhammad Siddiq, Nazish Manzoor

Turkish Journal of Botany

The aim of the present study was to investigate the effect of nutritional formulations, viz. Bristol's medium (BM), water extract of dairy waste (DW), commercial fertilizers mix (FM), and carbon sources such as NaHCO3, CaCO3, and wheat flour hydrolysate on the biomass productivity and oil content of the algal species Sirogonium sticticum, Temnogyra reflexa, Uronema elongatum, and Chroococcus turgidus. Among the nutritional formulations tested, BM showed the highest average effect, followed by DW, on the biomass and oil contents of the species. As for carbon sources, CaCO3 was more efficient in enhancing algal biomass, while NaHCO3 was proficient in increasing …