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Full-Text Articles in Life Sciences

Dna Protective Activity Of Peels Of Some Vegetables Wastes, Aisha Ishaq, Kainaat William Dec 2015

Dna Protective Activity Of Peels Of Some Vegetables Wastes, Aisha Ishaq, Kainaat William

Journal of Bioresource Management

The present study was conducted by using kitchen wastes like peels of different vegetables e.g. cucumber, potato, carrot, chickpea, cauliflower and pea, to evaluate the phytochemical constituents, DNA protection ability and free radicals scavenging activity. Phytochemicals tests showed the presence of carbohydrates, amino acids, glycosides, alkaloids, tannins and steroids in all samples while phlobatannins, anthraquonines and saponins were absent. The DNA protection of samples was checked by DNA protection assay using plasmid pBR322. Cauliflower and cucumber showed best protection at each dilution. DPPH free radicals scavenging assay was used to evaluate the antioxidant activity of peel extract of vegetables. Carrot …


Non-Canonical Activation Of Nrf2 By Dimercaptopropanol As A Treatment For Huntington's Disease, Margaret Lauren Tindale Oct 2015

Non-Canonical Activation Of Nrf2 By Dimercaptopropanol As A Treatment For Huntington's Disease, Margaret Lauren Tindale

Electronic Thesis and Dissertation Repository

Mitochondrial dysfunction and elevated reactive oxygen species (ROS) levels are strongly implicated in various neurodegenerative disorders, including Huntington’s disease (HD). Expression of the mutant Huntingtin protein (mHTT) containing an expanded polyglutamine repeat is associated with oxidative stress and toxicity in striatal neurons. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a redox-sensitive transcription factor responsible for regulating expression of a diverse array of antioxidant and cytoprotective genes. Most known Nrf2-activating compounds act through the canonical pathway by mimicking a transient oxidative insult, and treatment effects are short-lived. This study reveals an increase in striatal cell viability, and a reduction in …


Acute Exercise And Oxidative Stress: Crossfit™ Vs. Treadmill Bout, Brian Kliszczewicz, Quindry C. John, Blessing L. Daniel, Oliver D. Gretchen Oct 2015

Acute Exercise And Oxidative Stress: Crossfit™ Vs. Treadmill Bout, Brian Kliszczewicz, Quindry C. John, Blessing L. Daniel, Oliver D. Gretchen

Faculty and Research Publications

CrossFit™, a popular high-intensity training modality, has been the subject of scrutiny, with concerns of elevated risk of injury and health. Despite these concerns empirical evidence regarding physiologic stresses including acute oxidative stress is lacking. Therefore, the purpose of this investigation was to examine the acute redox response to a CrossFit™ bout. Furthermore, these findings were compared to a high-intensity treadmill bout as a point of reference. Ten males 26.4 ± 2.7 yrs having three or more months of CrossFit™ experience participated in the present study. Blood plasma was collected at four time points: Pre-exercise (PRE), immediately-post-exercise (IPE), 1 hr-post …


The Antioxidant And Dna Repair Capacities Of Resveratrol, Piceatannol, And Pterostilbene, Justin Ryan Livingston Jun 2015

The Antioxidant And Dna Repair Capacities Of Resveratrol, Piceatannol, And Pterostilbene, Justin Ryan Livingston

Theses and Dissertations

Lifestyle diseases represent a large burden on developed societies and account for much morbidity worldwide. Research has shown that eating a diet rich in fruit and vegetables helps to ameliorate and prevent some of these diseases. Antioxidants found in fruits and vegetables may provide a substantial benefit in reducing disease incidence. This thesis examines the antioxidant properties of resveratrol, piceatannol, and pterostilbene, and the ability of Burkitt's Lymphoma (Raji) cells to uptake these three antioxidants. It also studies the effect of the antioxidants in protecting against DNA damage and their role in DNA repair following oxygen radical exposure in Raji …


Effects Of Degree Of Roast And Application Form Of Incorporated Coffee On Inhibition Of Oxidation In Raw Refrigerated Minced Pork And Sensory Analysis Of Cooked Pork Patties With Added Coffee, Tiffany Hashimoto, Lilian Were, Criselda S. Toto, Fred Caporaso May 2015

Effects Of Degree Of Roast And Application Form Of Incorporated Coffee On Inhibition Of Oxidation In Raw Refrigerated Minced Pork And Sensory Analysis Of Cooked Pork Patties With Added Coffee, Tiffany Hashimoto, Lilian Were, Criselda S. Toto, Fred Caporaso

Student Scholar Symposium Abstracts and Posters

Roasted coffee is a source of antioxidants, but antioxidant Maillard reaction products and phenolic compound levels vary depending on degree of roast and form of coffee. The research objective was to evaluate the antioxidant effect of light and dark roasted coffee added to refrigerated minced pork (0.1 g/kg) as spent, ground, and lyophilized brewed coffee. After three weeks, all treated pork samples had TBARS values that were significantly lower than that of the negative control and comparable to rosemary. Metmyoglobin levels of meat treated with dark brew were higher than all other treatments after 3 weeks, while light brew had …


Evaluating Antioxidant Activity Of Selected Plant Species Native To Cedarville, Ohio, Daniel A. Benson, Alexander P. Treide, David Woodfield, Joshua A. Sitler, Denise S. Simpson, Robert L. Paris Apr 2015

Evaluating Antioxidant Activity Of Selected Plant Species Native To Cedarville, Ohio, Daniel A. Benson, Alexander P. Treide, David Woodfield, Joshua A. Sitler, Denise S. Simpson, Robert L. Paris

The Research and Scholarship Symposium (2013-2019)

Over the past several decades, there has been an increase in the number of synthetic drug molecules developed and utilized to treat various conditions. Although these synthetic drugs have proven useful, there has been growing public concern regarding the potentially negative long-term effects of synthetic agents on the body. As a result, there has been an increased interest in identifying and utilizing plant extracts and purified compounds since they are perceived to be a more natural alternative to synthetic drugs. The goal of this study was to evaluate the specific antioxidant properties of alsike clover Trifolum hybridum when produced under …


Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck Mar 2015

Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck

Feng Chen

Turmeric (Curcuma longa L.) plants that were grown in vitro for 17 or 22 weeks as a fed-batch culture in 2.5 L vessels yielded 39 to 43 g and 62 to 70 g of fresh rhizomes per vessel, respectively (95 % confidence interval). The MS liquid medium was maintained at 6 % sucrose through media addition twice during the experiment. Various methods were employed in attempts to increase secondary metabolism. Antioxidant and total phenolics assays were employed to characterize phytochemical activity. A first experiment exposed four clones to phenylalanine and/or methyl jasmonate (MeJa) from week 12 to 17 in culture. …


Exploring The Antibacterial, Antioxidant, And Anticancerproperties Of Lichen Metabolites, Gajendra Shrestha Mar 2015

Exploring The Antibacterial, Antioxidant, And Anticancerproperties Of Lichen Metabolites, Gajendra Shrestha

Theses and Dissertations

Natural products have been a significant source of new drugs, especially in treating cancer, infectious diseases, hypertension, and neurological disorders. Although many natural metabolites have been screened and yielded pharmaceutically important drugs, many potential sources of natural product drug therapies still need to be investigated, including lichens. Lichens are symbiotic systems consisting of a filamentous fungus and a photosynthetic partner (an eukaryotic alga and/or cyanobacterium). Lichens produce an impressive variety of unique secondary compounds and have been used as ingredients in folk medicines for centuries. Demonstrated biological roles based on lichen chemistry include: antibiotics, anti-proliferative, antioxidants, anti-HIV, anti-cancer, immunomodulation, and …


High-Density Lipoprotein Antioxidant Responses To High-Intensity Interval And Steady-State Moderate-Intensity Exercise, Zacharias Papadakis, Jeffrey S. Forsse, Annie A. Bane, Taylor Kyle, Li Qian, Flor Elisa Morales Marroquín, Peter W. Grandjean Feb 2015

High-Density Lipoprotein Antioxidant Responses To High-Intensity Interval And Steady-State Moderate-Intensity Exercise, Zacharias Papadakis, Jeffrey S. Forsse, Annie A. Bane, Taylor Kyle, Li Qian, Flor Elisa Morales Marroquín, Peter W. Grandjean

International Journal of Exercise Science: Conference Proceedings

High-intensity interval exercise (HIIE) may impart health benefits beyond what is acquired through moderate-intensity steady state exercise (SSE). Paraoxonase 1 (PON1), an antioxidant associated with high-density lipoprotein (HDL), may be altered with exercise; however, it is unclear whether HIIE provides a greater stimulus for increasing PON1 antioxidant activity than a comparable or greater amount of SSE. PURPOSE: Determine the influence of HIIE on PON1 concentration and activity relative to a comparable amount of moderate-intensity SSE and a dose that is half that of SSE. METHODS: Seventeen male participants (age 27.8 + 6.4 yr; weight 80.6 + 9.0 kg; BMI 25.1 …


Phytochemical Analysis And Biological Activities Of Cola Nitida Bark, Durand Dah-Nouvlessounon, Mamoudou H. Dicko Prof., Lamine Baba-Moussa Jan 2015

Phytochemical Analysis And Biological Activities Of Cola Nitida Bark, Durand Dah-Nouvlessounon, Mamoudou H. Dicko Prof., Lamine Baba-Moussa

Pr. Mamoudou H. DICKO, PhD

The aim of this study is to investigate some biological effects of Cola nitida’s bark after phytochemical screening. The bark was collected, dried, and then powdered for the phytochemical screening and extractions. Ethanol and ethyl acetate extracts of C. nitida were used in this study. The antibacterial activity was tested on ten reference strains and 28 meat isolated Staphylococcus strains by disc diffusionmethod. The antifungal activity of three fungal strains was determined on the Potato-Dextrose Agar medium mixed with the appropriate extract. The antioxidant activity was determined by DPPH and ABTS methods. Our data revealed the presence of various potent …


Impact Of Algae Supplemented Diets Combined With Antioxidants On The Nutritional Profile, Quality Attributes, And Storage Stability Of Chicken Breast Meat, Rebecca G. Norcross Jan 2015

Impact Of Algae Supplemented Diets Combined With Antioxidants On The Nutritional Profile, Quality Attributes, And Storage Stability Of Chicken Breast Meat, Rebecca G. Norcross

Theses and Dissertations--Animal and Food Sciences

Consumers’ demands for ω-3 polyunsaturated fatty acids (PUFAs) are at all-time high. Algae, a common source of PUFAs, and antioxidants are both used as supplements in livestock feeds, are known to affect the overall quality of meat. To implement PUFA deposits into broiler meat, this study evaluated combining antioxidants and algae in broiler feed to enhance the breast meat quality. Broilers were fed diets supplemented with 50 IU Vitamin E or 200 g/ton EconomasE (EcoE, an antioxidant pack) plus 10 IU Vitamin E, with or without 0.5% algae extract (SP-1). The feed oil was partially oxidized soybean oil (POV: 86 …


The Bioactive Compounds In Agricultural Products And Their Roles In Health Promoting Functions, Yixiao Shen Jan 2015

The Bioactive Compounds In Agricultural Products And Their Roles In Health Promoting Functions, Yixiao Shen

LSU Doctoral Dissertations

Bioactive compounds from different agricultural food products have attracted great interest from food industries and researchers for their health promoting functions such as antioxidant, antiaging, anti-inflammatory and anticancer performance. In this study, hydrophilic and lipophilic fraction of two economical agricultural products sweet sorghum millet and sweet potato, as well as two herbs, butterfly pea and basil were extracted. The profiles and contents of phenolics, fatty acids, tocopherols, carotenoids and phytosterols in these selected agricultural products were determined by chromatography and mass spectrum methods. Additionally, the anti-lipid-oxidation capability of sweet sorghum millet and basil, and anti-cancer potential of butterfly pea seed …


Antioxidant Activity Of Heme Oxygenase 1 In Brassica Juncea (L.) Czern.(Indian Mustard) Under Salt Stress, Khushbu Verma, Shubham Dixit, Gyan Singh Shekhawat, Afroz Alam Jan 2015

Antioxidant Activity Of Heme Oxygenase 1 In Brassica Juncea (L.) Czern.(Indian Mustard) Under Salt Stress, Khushbu Verma, Shubham Dixit, Gyan Singh Shekhawat, Afroz Alam

Turkish Journal of Biology

Antioxidant enzymes play a protective role in plants under oxidative stress. The present study explores the role of antioxidant system heme oxygenase1 (HO1) in Brassica juncea (L.) Czern. in salt stress conditions. B. juncea seedlings were treated with different NaCl concentrations (0-200 mM) for 5 days. Salt stress elicited the highest response at 150 mM NaCl concentration; at this level, gene expression of HO1 was also upregulated. Apart from HO1, ascorbate peroxidase (APX), peroxidase (POD), and glutathione reductase (GR) also showed their highest activity at 150 mM NaCl concentration. The study shows that HO1 is indispensable in B. juncea for …


The Modified Qualities Of Basil Plants By Selenium And/Or Ascorbic Acid, Zahra Oraghi Ardebili, Narges Oraghi Ardebili, Sara Jalili, Somayeh Safiallah Jan 2015

The Modified Qualities Of Basil Plants By Selenium And/Or Ascorbic Acid, Zahra Oraghi Ardebili, Narges Oraghi Ardebili, Sara Jalili, Somayeh Safiallah

Turkish Journal of Botany

The current research aimed to evaluate the possible impacts of foliar supplementations of selenium (Se) and/or ascorbic acid (AsA) on basil. Seedlings were foliarly treated with 4 concentrations of Se (0, 30, 60, and 120 mg L-1) and/or 2 levels of AsA (0 and 200 mg L-1), 2 times with 2-week intervals. In contrast to Se30, chla contents were significantly decreased by Se120 compared with the control. Se applications had a reducing effect on chlb contents. Se and/or AsA treated plants exhibited higher contents of carotenoid. The antioxidative system was affected by the applied treatments as it was indicated by …