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Articles 1 - 18 of 18
Full-Text Articles in Life Sciences
A Description Of Tolerance To Apical Meristem Damage In Wild Populations Of Arabidopsis Thaliana And Its Genetic Basis, Justin Dalrymple
A Description Of Tolerance To Apical Meristem Damage In Wild Populations Of Arabidopsis Thaliana And Its Genetic Basis, Justin Dalrymple
Biology Theses
Since plants cannot actively avoid herbivory, they rely on two primary strategies to maintain their fitness in the face of herbivore pressure: resistance—the deterrence of herbivory via physical and chemical means—and tolerance—recuperation of aboveground mass after herbivory. Although diverse groups have been shown to be tolerant to herbivory, mechanisms of tolerance are not yet well known. There is also very little known about the ecological relevance of tolerance strategies in wild populations. In Arabidopsis thaliana, the apical meristem suppresses growth of axillary meristems to a high degree under normal conditions, but a number of axillary meristemswill grow after apical meristem …
Arabidopsis Chromatin Remodeler Brahma: Its Functional Interplay With Polycomb Proteins And The Ref6 Histone Demethylase, Chenlong Li
Electronic Thesis and Dissertation Repository
BRAHMA (BRM) is a SWI/SNF-type chromatin remodeling ATPase that plays an important role in regulation of gene expression. Tri-methylation of lysine 27 on histone H3 (H3K27me3) is a histone modification that is associated with transcriptionally repressed genes and catalyzed by Polycomb Group (PcG) proteins. BRM has been proposed to antagonize the function of PcG proteins but the underlying molecular mechanism is unclear. To understand how BRM regulates the function of PcG proteins during plant development, a genome-wide analysis of H3K27me3 in brm mutant was performed using chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Loss of BRM leads to increased …
Changing The Substrate Specificity Of Arogenate Dehydratases (Adts) From Arabidopsis Thaliana., Megan Es Smith-Uffen
Changing The Substrate Specificity Of Arogenate Dehydratases (Adts) From Arabidopsis Thaliana., Megan Es Smith-Uffen
Electronic Thesis and Dissertation Repository
Phenylalanine (Phe), an essential aromatic amino acid, serves as a precursor for protein synthesis and a variety of secondary metabolites in plants. Two pathways are known for Phe biosynthesis. In the first, prephenate dehydratases (PDTs) convert prephenate to phenylpyruvate, which is transaminated to Phe. In the second, prephenate is transaminated to arogenate, which is converted to Phe by arogenate dehydratases (ADTs). ADTs and PDTs are structurally very similar, as are their substrates. Six ADTs (ADT1-ADT6) have been identified in Arabidopsis thaliana. ADT1 and ADT2 can recognize both prephenate and arogenate as substrates whereas ADT3-ADT6 are solely arogenate-accepting. Twenty ADT domain-swapping …
Overexpression Of Patatin-Related Phospholipase Aiiiβ Altered The Content And Composition Of Sphingolipids In Arabidopsis, Maoyin Li, Jennifer E. Markham, Xuemin Wang
Overexpression Of Patatin-Related Phospholipase Aiiiβ Altered The Content And Composition Of Sphingolipids In Arabidopsis, Maoyin Li, Jennifer E. Markham, Xuemin Wang
Department of Biochemistry: Faculty Publications
In plants, fatty acids are primarily synthesized in plastids and then transported to the endoplasmic reticulum (ER) for synthesis of most of the complex membrane lipids, including glycerolipids and sphingolipids. The first step of sphingolipid synthesis, which uses a fatty acid and a serine as substrates, is critical for sphingolipid homeostasis; its disruption leads to an altered plant growth. Phospholipase As have been implicated in the trafficking of fatty acids from plastids to the ER. Previously, we found that overexpression of a patatin-related phospholipase, pPLAIIIβ, resulted in a smaller plant size and altered anisotropic cell expansion. Here, we determined the …
Two Spotted Spider Mite (Tetranychus Urticae) Selection To Arabidopsis Thaliana, Huzefa Ratlamwala
Two Spotted Spider Mite (Tetranychus Urticae) Selection To Arabidopsis Thaliana, Huzefa Ratlamwala
Electronic Thesis and Dissertation Repository
Spider mite feeding on A. thaliana induces the production of indole glucosinolates (IGs), plant secondary metabolites that negatively affect mite performance. In this study I conducted selection experiments on A. thaliana with varying levels of IGs, to determine if mites could adapt to IGs and other defense compounds. After 12 months, mites reared on host with IGs performed significantly better on A. thaliana than mites maintained on beans. However, an adaptation cost was detected between selected mite lines and their ancestral host. The qRT-PCR data on different mite lines revealed that the detoxification genes previously identified may only be involved …
Regulation Of Mor By Different Abiotic Stresses In Arabidopsis Thaliana, Luke Stepan, Rucha Karve, Anjali Iyer-Pascuzzi
Regulation Of Mor By Different Abiotic Stresses In Arabidopsis Thaliana, Luke Stepan, Rucha Karve, Anjali Iyer-Pascuzzi
The Summer Undergraduate Research Fellowship (SURF) Symposium
The climate is changing and as a consequence the environment is becoming hotter and drier. How different plants will react to these changes is unknown. Identification of genes involved in stress tolerance can help predict plant-environment interactions and lead to stress tolerant plants. The MOR gene (Modulator Of Root ROS, ROS = Reactive Oxygen Species) in the model plant Arabidopsis thaliana encodes a transcription factor that may regulate stress responses, as mor mutants are drought tolerant. We hypothesized that MOR expression changes in response to different abiotic stress stimuli. We tested MOR expression in response to salt (NaCl), abscisic acid …
Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino
Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino
Theses and Dissertations - UTB/UTPA
In this study the functional characterization and overexpression of Metal Tolerance Protein (MTP) cDNA were evaluated using a transgenic yeast (Saccharomyces cerevisiae) model system as a preliminary means for the characterization of metal tolerance conferred by putative Group III MTP genes from Arabidopsis thaliana. Metal disc assays for nickel, zinc, cobalt and cadmium treatments were performed to measure the tolerance imparted to yeast by the MTP cDNAs. Statistical analysis from zinc and cadmium assays showed AtMTP1 imparted the highest zinc tolerance in yeast, while AtMTPB1 imparted the highest cadmium and cobalt tolerance. However, AtMTPA2 and AtMTPB produced a decrease in …
Analyses Of Catharanthus Roseus And Arabidopsis Thaliana Wrky Transcription Factors Reveal Involvement In Jasmonate Signaling, Craig Schluttenhofer, Sitakanta Pattanaik, Barunava Patra, Ling Yuan
Analyses Of Catharanthus Roseus And Arabidopsis Thaliana Wrky Transcription Factors Reveal Involvement In Jasmonate Signaling, Craig Schluttenhofer, Sitakanta Pattanaik, Barunava Patra, Ling Yuan
Plant and Soil Sciences Faculty Publications
BACKGROUND: To combat infection to biotic stress plants elicit the biosynthesis of numerous natural products, many of which are valuable pharmaceutical compounds. Jasmonate is a central regulator of defense response to pathogens and accumulation of specialized metabolites. Catharanthus roseus produces a large number of terpenoid indole alkaloids (TIAs) and is an excellent model for understanding the regulation of this class of valuable compounds. Recent work illustrates a possible role for the Catharanthus WRKY transcription factors (TFs) in regulating TIA biosynthesis. In Arabidopsis and other plants, the WRKY TF family is also shown to play important role in controlling tolerance to …
Characterization Of Udp-Arabinopyranose Mutase Genes In The Arabidopsis Cell Wall Mutant Mur5, Christopher A. Hart
Characterization Of Udp-Arabinopyranose Mutase Genes In The Arabidopsis Cell Wall Mutant Mur5, Christopher A. Hart
Honors Scholar Theses
The genome of Arabidopsis thaliana contains several coding regions for UDP-arabinopyranose mutases (UAMs) that are also known as reversibly glycosylated polypeptides (RGPs). The mur5 cell wall mutant of Arabidopsis shows a 30% decrease in cell wall arabinose content, and a missense mutation in the Reversibly Glycosylated Polypeptide 2 gene was recently proposed to cause this mutant phenotype. Through a traditional complementation analysis, mur5 and a T-DNA insertion mutant in the RGP2 gene were shown not to complement each other, indicating that the two genes are mutant alleles of the same locus. The mur5 SNP located in RGP2 caused a more …
Arabidopsis Thaliana-Spider Mite Interaction: Plant Perception, Signalling, And Response, Kristie A. Bruinsma
Arabidopsis Thaliana-Spider Mite Interaction: Plant Perception, Signalling, And Response, Kristie A. Bruinsma
Electronic Thesis and Dissertation Repository
The two-spotted spider mite, Tetranychus urticae, is a cell-content feeding chelicerate herbivore, feeding on over 1000 plant species, one of which is Arabidopsis thaliana. This research uses microarray data from two A. thaliana accessions that differ in susceptibility to spider mite feeding to identify how the plant defends itself against this herbivore. Mutant analysis of induced plant defense pathways and physiological assays of mite performance indicate that A. thaliana utilizes: a) damage associated molecular pattern receptors, PEPR1 and PEPR2, to aid in perception of attack; b) jasmonic acid as the key phytohormone involved in resistance signalling; and c) …
Comparison Of Five Major Trichome Regulatory Genes In Brassica Villosa With Orthologues Within The Brassicaceae, Naghabushana K. Nayidu, Sateesh Kagale, Ali Taheri, Thushan S. Withana-Gamage, Isobel A. P. Parkin, Andrew G. Sharpe, Margaret Y. Gruber
Comparison Of Five Major Trichome Regulatory Genes In Brassica Villosa With Orthologues Within The Brassicaceae, Naghabushana K. Nayidu, Sateesh Kagale, Ali Taheri, Thushan S. Withana-Gamage, Isobel A. P. Parkin, Andrew G. Sharpe, Margaret Y. Gruber
Agricultural and Environmental Sciences Faculty Research
Coding sequences for major trichome regulatory genes, including the positive regulators GLABRA 1(GL1), GLABRA 2 (GL2), ENHANCER OF GLABRA 3 (EGL3), and TRANSPARENT TESTA GLABRA 1 (TTG1) and the negative regulator TRIPTYCHON (TRY), were cloned from wild Brassica villosa, which is characterized by dense trichome coverage over most of the plant. Transcript (FPKM) levels from RNA sequencing indicated much higher expression of the GL2 and TTG1 regulatory genes in B. villosa leaves compared with expression levels of GL1 and EGL3 genes in either B. villosa or the reference genome species, glabrous B. oleracea; however, cotyledon TTG1 expression was high in …
The Role Of Mn-Sod And Fe-Sod Genes In The Response To Low Temperature In Chs Mutants Of Arabidopsis, Zahra Gharari, Ramazanali Khavari Nejad, Reza Shekaste Band, Farzane Najafi, Mohammad Nabiuni, Saeed Irian
The Role Of Mn-Sod And Fe-Sod Genes In The Response To Low Temperature In Chs Mutants Of Arabidopsis, Zahra Gharari, Ramazanali Khavari Nejad, Reza Shekaste Band, Farzane Najafi, Mohammad Nabiuni, Saeed Irian
Turkish Journal of Botany
To determine whether the expression of iron superoxide dismutase (Fe-SOD) and manganese superoxide dismutase (Mn-SOD) increase superoxide-scavenging capacity, and thereby improve the survival rate of chilling sensitive (chs) mutants of Arabidopsis, 4 chs mutant (chs1-1, chs1-2, chs2-1, and chs2-2) and wild-type plants were grown under low (chilling, 13 °C; cold, 4 °C) and normal growth (23 °C) temperatures. Photosynthetic parameters were investigated following treatment with chilling, cold, and normal growth conditions. Chlorophyll content and maximum quantum efficiency of PSII primary photochemistry (Fv/Fm) were reduced in plants grown at chilling stress. The degree of chilling sensitivity of chs1 mutant plants was …
Aba Signaling Is Necessary But Not Sufficient For Rd29b Transcriptional Memory During Successive Dehydration Stresses In Arabidopsis Thaliana, Laetitia Virlouvet, Yong Ding, Hiroaki Fujii, Zoya Avramova, Michael Fromm
Aba Signaling Is Necessary But Not Sufficient For Rd29b Transcriptional Memory During Successive Dehydration Stresses In Arabidopsis Thaliana, Laetitia Virlouvet, Yong Ding, Hiroaki Fujii, Zoya Avramova, Michael Fromm
School of Biological Sciences: Faculty Publications
Plants subjected to a prior dehydration stress were seen to have altered transcriptional responses during a subsequent dehydration stress for up to 5 days after the initial stress. The abscisic acid (ABA) inducible RD29B gene of Arabidopsis thaliana was strongly induced after the first stress and displayed transcriptional memory with transcript levels nine-fold higher during the second dehydration stress. These increased transcript levels were due to an increased rate of transcription and are associated with an altered chromatin template during the recovery interval between the dehydration stresses. Here we use a combination of promoter deletion/substitutions, mutants in the trans-acting …
Physiological And Transcriptional Memory In Guard Cells During Repetitive Dehydration Stress, Laetitia Virlouvet, Michael E. Fromm
Physiological And Transcriptional Memory In Guard Cells During Repetitive Dehydration Stress, Laetitia Virlouvet, Michael E. Fromm
Nebraska Center for Biotechnology: Faculty and Staff Publications
Arabidopsis plants subjected to a daily dehydration stress and watered recovery cycle display physiological and transcriptional stress memory. Previously stressed plants have stomatal apertures that remain partially closed during a watered recovery period, facilitating reduced transpiration during a subsequent dehydration stress. Guard cells (GCs) display transcriptional memory that is similar to that in leaf tissues for some genes, but display GC-specific transcriptional memory for other genes. The rate-limiting abscisic acid (ABA) biosynthetic genes NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3) and ALDEHYDE OXIDASE 3 (AAO3) are expressed at much higher levels in GCs, particularly during the watered recovery interval, relative to their low …
Development Of An Improved Rna Interference Vector System For Agrobacterium-Mediated Plant Transformation, Umut Toprak, Cathy Coutu, Doug Baldwin, Martin Erlandson, Dwayne Hegedus
Development Of An Improved Rna Interference Vector System For Agrobacterium-Mediated Plant Transformation, Umut Toprak, Cathy Coutu, Doug Baldwin, Martin Erlandson, Dwayne Hegedus
Turkish Journal of Biology
Plant-mediated RNA interference (RNAi) has shown a great potential in pest control and requires i) subcloning of sense/antisense regions in compatible vectors, ii) transfer of the silencing cassette into a binary vector, iii) transformation of Agrobacterium tumefaciens with desired binary plasmids, and iv) transformation of plants with Agrobacterium. The procedure is long and should ensure plasmid backbone stability; however, plasmid recombination due to antibiotic selection is a common problem. pGSA1252 is an RNAi silencing binary vector allowing direct cloning of hairpin structure; however, it possesses a chloramphenicol selection marker leading to plasmid recombination in various Agrobacterium strains. To solve this …
Comprehensive Analysis Of Beta-Galactosidase Protein In Plants Based On Arabidopsis Thaliana, Samin Seddigh, Maryam Darabi
Comprehensive Analysis Of Beta-Galactosidase Protein In Plants Based On Arabidopsis Thaliana, Samin Seddigh, Maryam Darabi
Turkish Journal of Biology
Beta-galactosidases (\BETAgals) (EC 3.2.1.23) have been detected in a wide range of plant organs and tissues and are described by their ability to hydrolyze terminal nonreducing \BETA-D-galactosyl residues from \BETA-D-galactosides. In this study, 92 \BETAgal protein sequences from different plants, 7 animal samples including human and mouse, 3 samples from bacteria including Escherichia coli, and 4 samples from insects including Drosophila melanogaster were aligned. Sequences were analyzed by computational tools to predict the protein properties, such as molecular mass, isoelectric point, signal peptide, motifs, transmembrane domain, and secondary and spatial structure. Protein structure analysis revealed there is a high identity …
H3k27me3 And H3k4me3 Chromatin Environment At Super-Induced Dehydration Stress Memory Genes Of Arabidopsis Thaliana, Ning Liu, Zoya Avramova, Michael Fromm
H3k27me3 And H3k4me3 Chromatin Environment At Super-Induced Dehydration Stress Memory Genes Of Arabidopsis Thaliana, Ning Liu, Zoya Avramova, Michael Fromm
School of Biological Sciences: Faculty Publications
Pre-exposure to a stress may alter the plant’s cellular, biochemical, and/or transcriptional responses during future encounters as a “memory” from the previous stress. Genes increasing transcription in response to a first dehydration stress, but producing much higher transcript levels in a subsequent stress, represent the super-induced “transcription memory” genes in Arabidopsis thaliana. The chromatin environment (histone H3 tri-methylations of Lys 4 and Lys 27, H3K4me3, and H3K27me3) studied at five dehydration stress memory genes revealed existence of distinct memory-response subclasses that responded differently to CLF deficiency and displayed different transcriptional activities during the watered recovery periods. Among the most …
Evolution Of A Plant-Specific Copper Chaperone Family For Chloroplast Copper Homeostasis., José Argüello, Crysten Blaby-Haas, Teresita Padilla-Benavides, Roland Stube, Sabeeha Merchant
Evolution Of A Plant-Specific Copper Chaperone Family For Chloroplast Copper Homeostasis., José Argüello, Crysten Blaby-Haas, Teresita Padilla-Benavides, Roland Stube, Sabeeha Merchant
José M. Argüello
Metallochaperones traffic copper (Cu(+)) from its point of entry at the plasma membrane to its destination. In plants, one destination is the chloroplast, which houses plastocyanin, a Cu-dependent electron transfer protein involved in photosynthesis. We present a previously unidentified Cu(+) chaperone that evolved early in the plant lineage by an alternative-splicing event of the pre-mRNA encoding the chloroplast P-type ATPase in Arabidopsis 1 (PAA1). In several land plants, recent duplication events created a separate chaperone-encoding gene coincident with loss of alternative splicing. The plant-specific Cu(+) chaperone delivers Cu(+) with specificity for PAA1, which is flipped in the envelope relative to …