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Purification And Characterization Of An Antibacterial Substance Produced By Pest-Originated Serratia Marcescens Mm3, Serpi̇l Uğraş, Kazim Sezen, Hati̇ce Kati, Zi̇hni̇ Demi̇rbağ
Purification And Characterization Of An Antibacterial Substance Produced By Pest-Originated Serratia Marcescens Mm3, Serpi̇l Uğraş, Kazim Sezen, Hati̇ce Kati, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The aim of this investigation was to determine, purify, and characterize an antibacterial substance produced by Serratia marcescens Mm3 (Sm-Mm3) isolated from a common pest of hazelnut in Turkey, Melolontha melolontha, which is also a significant pest all over the world. A highly active antibacterial substance produced by Sm-Mm3 was purified by ammonium sulfate precipitation and high-performance liquid chromatography, respectively, and identified by spectrometric analysis (TOF-MS). Finally, we showed that this molecule is heat-stable, stable in the pH range of 5-9, and insensitive to organic solvents and proteinase K. The molecular mass was determined as 479 Da. Plasmid elimination demonstrated …
Large-Scale Purification Of A Bacteriocin Produced By Leuconostoc Mesenteroides Subsp. Cremoris Using Diatomite Calcium Silicate, Hali̇l Dündar, Ömür Çeli̇kbiçak, Beki̇r Sali̇h, Tahsi̇n Faruk Bozoğlu
Large-Scale Purification Of A Bacteriocin Produced By Leuconostoc Mesenteroides Subsp. Cremoris Using Diatomite Calcium Silicate, Hali̇l Dündar, Ömür Çeli̇kbiçak, Beki̇r Sali̇h, Tahsi̇n Faruk Bozoğlu
Turkish Journal of Biology
Leuconostoc mesenteroides subsp. cremoris strain W3, isolated from wine, was previously found to produce a bacteriocin with inhibitory activity towards malolactic bacteria and hence inhibit malolactic fermentation in model wine medium. The bacteriocin, which we termed mesentericin W3, was purified by multistep chromatography and found in a previous study to be similar to mesentericin Y105, albeit with a low recovery (3.2%). In this study, we aimed to achieve large-scale isolation of mesentericin W3 by adsorption of the bacteriocin from culture supernatant onto Micro-Cel (diatomite calcium silicate) and then by subsequent desorption. Water-soluble surfactants including Tween 80, Triton X-100, sodium deoxycholate, …
Inhibitory Effect Of Enterocin Kp In Combination With Sublethal Factors On Escherichia Coli O157:H7 Or Salmonella Typhimurium In Bhi Broth And Uht Milk, Zeli̇ha Yildirim, Yaseli̇n İlk, Meti̇n Yildirim, Kader Tokatli, Ni̇lgün Öncül
Inhibitory Effect Of Enterocin Kp In Combination With Sublethal Factors On Escherichia Coli O157:H7 Or Salmonella Typhimurium In Bhi Broth And Uht Milk, Zeli̇ha Yildirim, Yaseli̇n İlk, Meti̇n Yildirim, Kader Tokatli, Ni̇lgün Öncül
Turkish Journal of Biology
The effects of physical and chemical sublethal treatments on the antibacterial activity of enterocin KP produced by Enterococcus faecalis KP against Escherichia coli O157:H7 and Salmonella Typhimurium were investigated. Enterocin KP was not active against intact cells of E. coli O157:H7 or S. Typhimurium. However, the use of enterocin KP together with ethylenediaminetetraacetic acid (50 mM), sodium tripolyphosphate (50 mM), sublethal heating (60 °C for 10 min), cold shock (-20 °C for 2 h), or acid stress (mixture of 40% lactic acid, 16% propionic acid, 16% acetic acid) in BHI medium decreased the cell number of E. coli O157:H7 by …