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Articles 1 - 30 of 106
Full-Text Articles in Life Sciences
Cobalt Induces Alterations In Serum Parameters Associated With Bone Metabolism In Male Adult Rat, Ali Asghar Moshtaghie, Pedram Malekpouri, Elham Moshtaghie, Farhang Rahnamaie
Cobalt Induces Alterations In Serum Parameters Associated With Bone Metabolism In Male Adult Rat, Ali Asghar Moshtaghie, Pedram Malekpouri, Elham Moshtaghie, Farhang Rahnamaie
Turkish Journal of Biology
The intention of this study was to investigate the toxic effect of cobalt on the bone metabolism of rats, including calcium, inorganic phosphorus, and bone-specific alkaline phosphatase of serum during short- and long-term experiments. Rats were injected intraperitoneally (IP) with either 16 mg kg^{-1} BW of cobalt for 5 and 10 days or 6 mg kg^{-1} BW for 20 and 40 days. It was found that IP administrations of cobalt could increase all serum parameters associated with bone metabolic processes. The observed effect was greatest for 16 mg kg^{-1} BW when used for 10 continuous days. By using gel filtration …
Noncoding Rnas And Cancer, Hesna Begüm Akman Tuncer, Ayşe Eli̇f Erson Bensan
Noncoding Rnas And Cancer, Hesna Begüm Akman Tuncer, Ayşe Eli̇f Erson Bensan
Turkish Journal of Biology
New and diverse groups of noncoding RNAs are beginning to be discovered. These noncoding RNAs are grouped based on their sizes, genomic positions, or distinctive functions ranging from the regulation of chromatin structure and gene expression to genome stability maintenance. Among noncoding RNAs, microRNAs are the best studied and understood small RNAs with direct and indirect roles in normal and in cancer cells. Given the high rate of transcription from noncoding parts of the genome, other noncoding elements are also of great interest. It is also of interest to understand that there may be interesting cases of crosstalk and/or coregulation …
Heterotic Grouping And Patterning Of Quality Protein Maize Inbreds Based On Genetic And Molecular Marker Studies, Ambika Rajendran, Arunachalam Muthiah, John Joel, Ponnusamy Shanmugasundaram, Dhandapani Raju
Heterotic Grouping And Patterning Of Quality Protein Maize Inbreds Based On Genetic And Molecular Marker Studies, Ambika Rajendran, Arunachalam Muthiah, John Joel, Ponnusamy Shanmugasundaram, Dhandapani Raju
Turkish Journal of Biology
An investigation was done to study the heterotic grouping and patterning in quality protein maize inbreds. Biochemical screening resulted in the choice of 3 inbreds each with high (UQPM 2, UQPM 4, and UQPM 21) and low (UQPM 18, UQPM 19, and UQPM 20) lysine and tryptophan contents respectively for genetic studies using diallel analysis. UQPM 20 × UQPM 18 was notable as it possessed high standard heterosis and specific combining (sca) effect for grain yield, protein, tryptophan, and lysine. Based on yield sca, the 6 parental inbreds were classified into 3 heterotic groups. Intergroup cross UQPM 20 × UQPM …
Isolation And Amplification Of Genomic Dna From Barks Of Cinnamomum Spp., Valya Parambil Swetha, Viswanath Alambath Parvathy, Thotten Elampillay Sheeja, Bhaskaran Sasikumar
Isolation And Amplification Of Genomic Dna From Barks Of Cinnamomum Spp., Valya Parambil Swetha, Viswanath Alambath Parvathy, Thotten Elampillay Sheeja, Bhaskaran Sasikumar
Turkish Journal of Biology
Cinnamomum verum Presl (syn. C. zeylanicum Blume), the cinnamon of commerce, is an important aromatic tree spice having wide applications in perfumery, flavoring, beverages, and medicine. Adulteration of cinnamon with the cheaper and inferior barks of C. aromaticum and C. malabatrum is a problem. Morphological distinction of the barks is difficult; in the case of powdered barks, the situation is even worse. DNA-based molecular tools are preferred under these circumstances. Isolation of high quality DNA is a prerequisite for molecular studies, but barks contain polysaccharides, polyphenols, and secondary metabolites that hamper DNA isolation. Since attempts at isolating DNA using existing …
The Modeled Structures Of Deg5 And Deg8 Proteases In Arabidopsis Thaliana, Liangbing Chen, Qingzhi Li, Lili Li
The Modeled Structures Of Deg5 And Deg8 Proteases In Arabidopsis Thaliana, Liangbing Chen, Qingzhi Li, Lili Li
Turkish Journal of Biology
Deg5 and Deg8 are chloroplastic proteases found in Arabidopsis thaliana. These proteases participate in the repair of photosystem II. However, little information exists on the structures of Deg5 and Deg8. Here, the Deg5 and Deg8 structures were modeled using the SWISS-MODEL tool with Deg1 as the structural template. Deg5 was composed of only 1 domain, a protease domain. The catalytic triad of Deg5, which comprises His147, Asp188, and Ser266, was located in the cleft between 2 \beta-barrels. Deg8 was composed of a protease domain at the N-terminal and a PDZ domain at the C-terminal. The catalytic triad of Deg8, which …
Improvement In Tolerance To Salt Stress During Tomato Cultivation, Mustafa Demi̇rkaya
Improvement In Tolerance To Salt Stress During Tomato Cultivation, Mustafa Demi̇rkaya
Turkish Journal of Biology
This study was conducted to evaluate the physiological impact of seed priming on improving the salt tolerance of tomato cultivars H-2274 and Rio Grande. Tomato seeds were primed with 5 M NaCl solution at 20 °C for 3 days in a dark environment. The seeds of the control treatments were handled in a similar way, using tap water instead of NaCl solution. After the priming procedure, seeds were washed and sown in standard germination trays. From each treatment group, 21 homogeneous seedlings were transplanted into 10-L pots full of turf for subsequent salinity experiments. In order to avoid osmotic shock, …
Transgenic Nicotiana Tabacum Cultivar Samsun Plants Carrying The Wild Sugar Beet Hs1pro1 Gene Have Resistance To Root-Knot Nematodes, Çağla Sönmez, İbrahi̇m Hali̇l Elekci̇oğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Transgenic Nicotiana Tabacum Cultivar Samsun Plants Carrying The Wild Sugar Beet Hs1pro1 Gene Have Resistance To Root-Knot Nematodes, Çağla Sönmez, İbrahi̇m Hali̇l Elekci̇oğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Nematodes are the principal animal parasites of plants, causing annual crop losses of more than US$100 billion worldwide. Conventional control measures against nematode infection include toxic nematicide application to soil, crop rotation practices, and classical breeding approaches. However, due to the limitations of each technique, biotechnology presents itself as an effective alternative in nematode control. To date, several resistance genes against nematodes have been cloned. One such gene, Hs1^{pro1}, cloned from wild sugar beet, Beta procumbens, shows resistance to the cyst nematode Heterodera schachtii. Recent studies showed that Hs1^{pro1} has a broad target pathogen range. In this study, we transformed …
Soil And Foliar Application Of Potassium Enhances Fruit Yield And Quality Of Tomato Under Salinity, Muhammad Amjad, Javaid Akhtar, Muhammad Anwar Ul Haq, Shakeel Imran, Sven-Erik Jacobsen
Soil And Foliar Application Of Potassium Enhances Fruit Yield And Quality Of Tomato Under Salinity, Muhammad Amjad, Javaid Akhtar, Muhammad Anwar Ul Haq, Shakeel Imran, Sven-Erik Jacobsen
Turkish Journal of Biology
Potassium plays a key role in the survival of plants under saline conditions in mitigating the adverse effects of sodium. The effect of application of potassium to soil (0, 3.3, and 6.6 mmol/kg) and leaves (4.5 and 9 mM) on tomato yield and quality under 3 salinity treatments (0, 7.5, and 15 dS m^{-1}), using 2 salt-tolerant (Indent-1 and Nagina) and 2 salt-sensitive (Peto-86 and Red Ball) genotypes, was studied in a pot experiment. Salinity decreased growth and yield of all genotypes; however, salt-tolerant genotypes maintained better growth and produced higher yield than the salt-sensitive genotypes across all salinity levels. …
Does Cyp1a1 Gene Polymorphism Affect Cell Damage Biomarkers And Ageing?, Sima Ataollahi Eshkoor, Patimah Ismail, Sabariah Abd Rahman
Does Cyp1a1 Gene Polymorphism Affect Cell Damage Biomarkers And Ageing?, Sima Ataollahi Eshkoor, Patimah Ismail, Sabariah Abd Rahman
Turkish Journal of Biology
This study aimed to investigate the effects of cytochrome P450 1A1 (CYP1A1) gene polymorphism on early ageing due to occupational exposure to mutagens. The project was conducted on buccal epithelial cells collected from mechanical-workshop workers and controls. Epithelial cells were obtained from the inner part of the cheeks. Techniques including comet assay, micronuclei test, real-time polymerase chain reaction, and restriction fragment length polymorphism-polymerase chain reaction were used to assess DNA damage biomarkers. The results showed that there was a significant difference in relative telomere length (P = 0.039) between wild and mutated genotypes among all individuals. Furthermore, occupational exposure showed …
Identification And Expression Profiling Of Clcuv-Responsive Transcripts In Upland Cotton (Gossypium Hirsutum L.), Beenish Aftab, Muhammad Naveed Shahid, Sania Riaz, Adil Jamal, Bahaeldeen Babiker Mohamed, Muzna Zahur, Mahwish Aftab, Bushra Rashid, Tayyab Husnain
Identification And Expression Profiling Of Clcuv-Responsive Transcripts In Upland Cotton (Gossypium Hirsutum L.), Beenish Aftab, Muhammad Naveed Shahid, Sania Riaz, Adil Jamal, Bahaeldeen Babiker Mohamed, Muzna Zahur, Mahwish Aftab, Bushra Rashid, Tayyab Husnain
Turkish Journal of Biology
CLCuV is one of the most serious causes of cotton damage for which no adequate remedy is available. The possible solution to this problem may be the transfer of resistant genes into the background of agronomical CLCuV-tolerant varieties. In response to viral infection, several plants may show qualitative and quantitative variations in gene expression. To study gene expression between diseased and tolerant plants, DDRT-PCR was performed. Upregulated gene expression was observed among cDNA transcripts screened through random primers. Seven transcripts have significant homology with known proteins (ribosomal protein, photosystem protein unit, membrane protein, and a predicted hypothetical protein). Quantitative real-time …
Effect Of Water Deficiency On Seed Quality And Physiological Traits Of Different Safflower Genotypes, Hajar Amini, Ahmad Arzani, Mostafa Karami
Effect Of Water Deficiency On Seed Quality And Physiological Traits Of Different Safflower Genotypes, Hajar Amini, Ahmad Arzani, Mostafa Karami
Turkish Journal of Biology
Safflower is one of the most adapted oilseed crops to the drought-prone arid lands. This experiment was carried out to evaluate the effects of water deficiency on some physiological traits such as proline content, soluble carbohydrate content, relative water content (RWC), and seed quality using 64 safflower genotypes grown under normal and water-deficient field conditions. Plants were grown under normal irrigation until the branching growth stage, when water deficiency was applied to the plants. Results of combined analysis of variance showed the significant effects of genotype, water deficiency, and their interactions on the tested traits. RWC, seed oil content, and …
Genome-Wide Identification And Analysis Of Growth Regulating Factor Genes In Brachypodium Distachyon: In Silico Approaches, Ertuğrul Fi̇li̇z, İbrahi̇m Koç, Hüseyi̇n Tombuloğlu
Genome-Wide Identification And Analysis Of Growth Regulating Factor Genes In Brachypodium Distachyon: In Silico Approaches, Ertuğrul Fi̇li̇z, İbrahi̇m Koç, Hüseyi̇n Tombuloğlu
Turkish Journal of Biology
Growth-regulating factor (GRF) genes may play important roles for regulating growth and development in different plant tissues and organs. Here we report the first genome-wide analysis of the GRF gene family in Brachypodium. We performed in silico comparative analysis of GRF genes, including their structure, duplication in the genome, conserved motifs, and phylogenetic relationship. At the end of the study, 10 BdGRF genes were identified. The highest number of GRF genes was identified on chromosome 1 with 5 members, whereas the least number of genes (only 1 member) was found on chromosomes 2, 4, and 5. Of those, a single …
Activity Of Antioxidant Enzymes During Induction Of Morphogenesis Of Fritillaria Meleagris In Bulb Scale Culture, Marija Petric, Sladana Jevremovic, Milana Trifunovic, Vojin Tadic, Snezana Milosevic, Angelina Subotic
Activity Of Antioxidant Enzymes During Induction Of Morphogenesis Of Fritillaria Meleagris In Bulb Scale Culture, Marija Petric, Sladana Jevremovic, Milana Trifunovic, Vojin Tadic, Snezana Milosevic, Angelina Subotic
Turkish Journal of Biology
We investigated the activity of 3 antioxidant enzymes: superoxide dismutase, catalase, and peroxidase during morphogenesis of Fritillaria meleagris L. in vitro in bulb scale culture. Bulb cultures of F. meleagris were established on nutrition medium supplemented with 1.0 mg L^{-1} thidiazuron. Bulbs were grown at standard (24 °C) and low temperatures (4 °C) or at an increased sucrose concentration (4.5%) during the first 6 weeks (pretreatments). All bulbs were then grown for 4 weeks at standard temperature on 2, 4-dichlorophenoxyacetic acid and kinetin or thidiazuron (at concentrations of 1 mg L^{-1} each) in order to examine the influence of the …
Y Chromosome Analysis Of Native Turkish Cattle Breeds By Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Ni̇zamlioğlu
Y Chromosome Analysis Of Native Turkish Cattle Breeds By Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Ni̇zamlioğlu
Turkish Journal of Biology
The aim of this study was to determine phylogenetic relationships of 6 native cattle breeds of Turkey using 7 Y chromosome-specific microsatellite DNA markers. DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red, and Zavot cattle using a standard phenol/chloroform method. PCR products were separated by capillary electrophoresis and marker genotypes were determined. A total of 41 different alleles were observed. The mean allele number was 5.86 and mean F_{IS} value was 0.427 for all populations. The INRA189 locus was monomorphic for all populations except for South Anatolian Red. UMN0307 …
Hepatoprotective Effects Of Malva Sylvestris L. Against Paracetamol-Induced Hepatotoxicity, Liaqat Hussain, Javaria Ikram, Kanwal Rehman, Muhammad Tariq, Muhammad Ibrahim, Muhammad Sajid Hamid Akash
Hepatoprotective Effects Of Malva Sylvestris L. Against Paracetamol-Induced Hepatotoxicity, Liaqat Hussain, Javaria Ikram, Kanwal Rehman, Muhammad Tariq, Muhammad Ibrahim, Muhammad Sajid Hamid Akash
Turkish Journal of Biology
Malva sylvestris is traditionally used for the treatment of liver diseases, but sufficient pharmacological-based scientific literature is not available online to authenticate its use in liver ailments. We aimed to assess the hepatoprotective effects of Malva sylvestris against paracetamol-induced hepatotoxicity in mice. The extract was concentrated using a rotary evaporator and then desired concentrations of extracts were made by dissolving in normal saline. The standard drug silymarin (100 mg/kg) was used as a reference drug to compare the therapeutic effects of Malva sylvestris. Two different doses of Malva sylvestris (300 and 600 mg/kg) were administered intraperitoneally for 7 consecutive days …
Herbicide-Tolerant Sugarcane (Saccharum Officinarum L.) Plants: An Unconventional Method Of Weed Removal, Idrees Ahmad Nasir, Bushra Tabassum, Zahida Qamar, Muhammad Aslam Javed, Muhammad Tariq, Abdul Munim Farooq, Shahid Javed Butt, Abdul Qayyum, Tayyab Husnain
Herbicide-Tolerant Sugarcane (Saccharum Officinarum L.) Plants: An Unconventional Method Of Weed Removal, Idrees Ahmad Nasir, Bushra Tabassum, Zahida Qamar, Muhammad Aslam Javed, Muhammad Tariq, Abdul Munim Farooq, Shahid Javed Butt, Abdul Qayyum, Tayyab Husnain
Turkish Journal of Biology
Unnecessary weed growth in sugarcane fields forces plants to compete for nutrients and sunlight for survival, which most often leads to significant yield losses. As chemical herbicides cannot differentiate between crop plants and weeds, the development of herbicide-tolerant crops is anticipated. Four sugarcane varieties, CPF-234, CPF-213, HSF-240, and CPF-246, were used to develop glyphosate herbicide tolerance. A glyphosate-tolerant gene of 1368 bp cloned directionally under the 35S promoter with the \Beta-glucuronidase (GUS) reporter gene was used as the transgene. Through the biolistic transformation of sugarcane, calli of all cultivars were transformed with glyphosate-tolerant gene constructs. Efficient regeneration conditions were optimized …
Peripheral Administration Of Kisspeptin Antagonist Does Not Alter Basal Plasma Testosterone But Decreases Plasma Adiponectin Levels In Adult Male Rhesus Macaques, Tanzeel Huma, Farhad Ulla, Farnaz Hanif, Joshua D. Rizak, Muhammad Shahab
Peripheral Administration Of Kisspeptin Antagonist Does Not Alter Basal Plasma Testosterone But Decreases Plasma Adiponectin Levels In Adult Male Rhesus Macaques, Tanzeel Huma, Farhad Ulla, Farnaz Hanif, Joshua D. Rizak, Muhammad Shahab
Turkish Journal of Biology
Kisspeptin signaling is a potent regulator of the reproductive axis and an elicitor of gonadotropin-releasing hormone secretion. The present study assessed the effect of the peripheral administration of a kisspeptin antagonist (P234) on testosterone secretion in the adult male rhesus monkey. Five monkeys were administered with either P234 (38.8 µg/kg body weight) or a vehicle (0.9% saline). Plasma testosterone and adiponectin levels, as a positive control, were then measured using specific ELISA assays from blood samples collected sequentially at 15-min intervals from 60 min prior to until 360 min after the P234/vehicle injection. In 3 monkeys, the experiment was repeated …
Melatonin Is Effective In Reducing Stress-Induced Organ Damage In Wistar Albino Rats, Mukaddes Eşrefoğlu, Ayşi̇n Akinci Bak, Hülya Elbe, Eli̇f Taşlidere, Asli Çeti̇n, Burhan Ateş
Melatonin Is Effective In Reducing Stress-Induced Organ Damage In Wistar Albino Rats, Mukaddes Eşrefoğlu, Ayşi̇n Akinci Bak, Hülya Elbe, Eli̇f Taşlidere, Asli Çeti̇n, Burhan Ateş
Turkish Journal of Biology
In the present study, we tried to investigate the effects of melatonin, a novel antioxidant and a potent free radical scavenger, in stress-induced cerebral, cerebellar, cardiac, and hepatic oxidative damage using microscopic and biochemical analysis. A total of 32 male Wistar albino rats were divided into control, stress, stress + saline, and stress + melatonin groups. The rats from the stress groups were exposed to high stress conditions of starvation, immobilization, and cold exposure. The rats from the stress + melatonin group received melatonin daily at 20 mg/kg body weight intraperitoneally for 7 days. At the end of the experiment, …
Polyploidy And Apomixis In Accessions Of Senna Rugosa (G.Don) H.S.Irwin & Barneby, Katia Resende, Clara Prado, Lisete Davide, Giovana Torres
Polyploidy And Apomixis In Accessions Of Senna Rugosa (G.Don) H.S.Irwin & Barneby, Katia Resende, Clara Prado, Lisete Davide, Giovana Torres
Turkish Journal of Biology
Senna rugosa (G.Don) H.S.Irwin & Barneby (Leguminosae) is a species from the Brazilian Cerrado commonly used for medicinal purposes. Chromosome variability (n = 14 and 28) described for this species indicates the occurrence of chromosome races and recent polyploidization in its evolution history. The aim of this study was to verify the relationship between ploidy level and occurrence of polyembryony in 5 accessions of S. rugosa. Cytogenetic analysis revealed the type of interphasic nuclei, chromosome number and morphology, and nuclear DNA content. Frequency of polyembryony was estimated by germination test. Pollen grain viability was estimated by different techniques. There was …
Styrian Oil Pumpkin Pollen Germinability At Higher Irradiation Doses: Optimization Of The In Vitro Germination Protocol And Irradiation Procedure, Kristina Kosmrlj, Damijana Kastelec, Borut Bohanec
Styrian Oil Pumpkin Pollen Germinability At Higher Irradiation Doses: Optimization Of The In Vitro Germination Protocol And Irradiation Procedure, Kristina Kosmrlj, Damijana Kastelec, Borut Bohanec
Turkish Journal of Biology
Protocols for haploid induction in cucurbits are based on pollination with irradiated pollen, but the induction frequency is low and the majority of obtained embryos are zygotic. The longevity of Cucurbita pepo L. pollen is short even under natural conditions; following irradiation, germinability is decreased even further. This study was initiated to develop an optimal in vitro germination medium for styrian oil pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) pollen, which would enable accurate germination testing. Different pH values and the addition of sucrose, mannitol, and polyethylene glycol to the Brewbaker and Kwack germination medium were tested. The …
Optimization Of E. Coli Culture Conditions For Efficient Dna Uptake By Electroporation, Irfan Ahmad, Tehseen Rubbab, Farah Deeba, Syed Muhammad Saqlan Naqvi
Optimization Of E. Coli Culture Conditions For Efficient Dna Uptake By Electroporation, Irfan Ahmad, Tehseen Rubbab, Farah Deeba, Syed Muhammad Saqlan Naqvi
Turkish Journal of Biology
Bacterial transformation is a significant breakthrough in the field of molecular biology for cloning purposes. A variety of chemical and physical methods are utilized for enhancing bacterial competence. The present study was designed, via the consolidation of chemical and physical methods, to improve competence of cells for high transformation efficiency up to 10^9 transformants/µg of DNA. LB and SOC media and their supplemented combinations with osmotic agents (sucrose and glycine) were used to prepare electrocompetent cells of E. coli DH5\alpha at temperatures of 25 and 37 °C, which were then subjected to electroporation. The results showed that cells grown in …
Reproductive Biology Study Of Dynamics Of Female Sexual Hormones: A 12-Month Exposure To Lead Acetate Rat Model, Eugenia Dumitrescu, Romeo Teodor Cristina, Florin Muselin
Reproductive Biology Study Of Dynamics Of Female Sexual Hormones: A 12-Month Exposure To Lead Acetate Rat Model, Eugenia Dumitrescu, Romeo Teodor Cristina, Florin Muselin
Turkish Journal of Biology
In human and animal organisms, lead can cause reproductive problems beginning with pregnant females. The reproductive axis is particularly sensitive to lead, its influence resulting in a delayed sexual maturity due to biosynthesis suppression of the sexual steroids. An animal model study was carried out on 28 white Wistar adult female rats, divided into 3 experimental (E) groups that were exposed for 12 months to lead acetate in drinking water as follows: 50 ppb Pb (E_1), 100 ppb Pb (E_2), and 150 ppb Pb (E_3), with a control group (M) that received unleaded tap water. Levels of FSH, LH, estradiol, …
Characterization Of A Novel Xylose Isomerase From Anoxybacillus Gonensis G2^T, Derya Yanmiş, Hakan Karaoğlu, Di̇lşat Ni̇gar Çolak, Fulya Ay Şal, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz
Characterization Of A Novel Xylose Isomerase From Anoxybacillus Gonensis G2^T, Derya Yanmiş, Hakan Karaoğlu, Di̇lşat Ni̇gar Çolak, Fulya Ay Şal, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz
Turkish Journal of Biology
The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2^T has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography techniques to homogeneity, and the biochemical properties of the enzyme were characterized. The optimum temperature of the enzyme was 85 °C and maximum activity was observed at a pH of 6.5. Its Km and Vmax values were calculated as 25 ± 2 mM and 0.12958 ± 0.002 mumol/min/mg protein, respectively. The effects of various metal ions on the xylose isomerase were examined. Divalent cations Co^{2+}, Mg^{2+}, and Mn^{2+} were essential …
Large-Scale Purification Of A Bacteriocin Produced By Leuconostoc Mesenteroides Subsp. Cremoris Using Diatomite Calcium Silicate, Hali̇l Dündar, Ömür Çeli̇kbiçak, Beki̇r Sali̇h, Tahsi̇n Faruk Bozoğlu
Large-Scale Purification Of A Bacteriocin Produced By Leuconostoc Mesenteroides Subsp. Cremoris Using Diatomite Calcium Silicate, Hali̇l Dündar, Ömür Çeli̇kbiçak, Beki̇r Sali̇h, Tahsi̇n Faruk Bozoğlu
Turkish Journal of Biology
Leuconostoc mesenteroides subsp. cremoris strain W3, isolated from wine, was previously found to produce a bacteriocin with inhibitory activity towards malolactic bacteria and hence inhibit malolactic fermentation in model wine medium. The bacteriocin, which we termed mesentericin W3, was purified by multistep chromatography and found in a previous study to be similar to mesentericin Y105, albeit with a low recovery (3.2%). In this study, we aimed to achieve large-scale isolation of mesentericin W3 by adsorption of the bacteriocin from culture supernatant onto Micro-Cel (diatomite calcium silicate) and then by subsequent desorption. Water-soluble surfactants including Tween 80, Triton X-100, sodium deoxycholate, …
Cloning, Purification, And Characterization Of A Thermophilic Ribulokinase From Anoxybacillus Kestanbolensis Ac26sari, Müslüm Tokgöz, Kadri̇ye İnan, Ali̇ Osman Beldüz, Öznur Gedi̇kli̇, Sabri̇ye Çanakçi
Cloning, Purification, And Characterization Of A Thermophilic Ribulokinase From Anoxybacillus Kestanbolensis Ac26sari, Müslüm Tokgöz, Kadri̇ye İnan, Ali̇ Osman Beldüz, Öznur Gedi̇kli̇, Sabri̇ye Çanakçi
Turkish Journal of Biology
The gene encoding ribulokinase araB from Anoxybacillus kestanbolensis AC26Sari was cloned and sequenced. The recombinant protein was expressed in Escherichia coli BL21 under the control of isopropyl-\beta-D-thiogalactopyranoside-inducible T7 promoter. The enzyme, designated as AC26RK, was purified with the MagneHis Protein Purification System. The molecular mass of the native protein, as determined by SDS-PAGE, was about 61 kDa. AC26RK was active throughout a broad pH (pH 5.0-10.0) and temperature (50-75 °C) range, and it had an optimum pH of 9.0 and optimum temperature of 60 °C. The enzyme displayed about 90%-100% of its original activities after a 30-min incubation at a …
Bacterial Toxin Colicin N-T Domain Structure Changes To Ordered State Upon Binding C-Terminal Domain Of Tola, Yakup Ulusu, Sema Bi̇lgi̇n Şentürk, Fatma Gedi̇kli̇, Jeremy H. Lakey, İsa Gökçe
Bacterial Toxin Colicin N-T Domain Structure Changes To Ordered State Upon Binding C-Terminal Domain Of Tola, Yakup Ulusu, Sema Bi̇lgi̇n Şentürk, Fatma Gedi̇kli̇, Jeremy H. Lakey, İsa Gökçe
Turkish Journal of Biology
Colicin N is a bacterial toxin that kills Escherichia coli and related cells. Its mode of action is of interest in protein import and toxicology. Colicin N translocates across the E. coli outer membrane and periplasm by interacting with several receptors. The translocation process involves the interaction of the colicin N with the outer membrane porin OmpF and subsequently with the integral membrane protein TolA. The N-terminal domain of colicin N is involved in the import process. TolA consists of 3 domains. The N-terminal domain of colicin N interacts with the C-terminal domain of TolA at later stages of the …
Biodegradation Of The Synthetic Pyrethroid Insecticide Α-Cypermethrin By Stenotrophomonas Maltophilia Og2, Özlem Gür, Murat Özdal, Ömer Faruk Algur
Biodegradation Of The Synthetic Pyrethroid Insecticide Α-Cypermethrin By Stenotrophomonas Maltophilia Og2, Özlem Gür, Murat Özdal, Ömer Faruk Algur
Turkish Journal of Biology
A novel gram-negative bacterium, OG2, was isolated from the body microflora of cockroaches (Blatta orientalis). Based on morphological, biochemical, and 16S ribosomal DNA sequence analysis, the isolated strain OG2 was identified as Stenotrophomonas maltophilia. This bacterial strain was screened for its alpha-cypermethrin-degrading potential with minimal salt medium (MSM). The alpha-cypermethrin degradation and utilization ability of the isolated organism was verified. Bacterial growth was measured by optical density in the presence of the pesticide at different concentrations (50-200 mg/L). S. maltophilia OG2 utilized alpha-cypermethrin as the sole carbon source for growth, and alpha-cypermethrin degradation increased when MSM was supplemented with glucose. …
Nitrate And Iron Nutrition Effects On Some Nitrate Assimilation Enzymes And Metabolites In Spirulina Platensis, Merve Esen, Razi̇ye Öztürk Ürek
Nitrate And Iron Nutrition Effects On Some Nitrate Assimilation Enzymes And Metabolites In Spirulina Platensis, Merve Esen, Razi̇ye Öztürk Ürek
Turkish Journal of Biology
Effects of different sodium nitrate and iron concentrations on the production of some metabolites and pigments and the activities of some nitrate assimilation enzymes [nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), and glutamate synthase (GOGAT)] were investigated in the growth medium of Spirulina platensis (Gamont) Geitler. For this purpose, S. platensis was cultivated on Zarrouk's medium containing different sodium nitrate concentrations (10-180 mM). A 100 mM sodium nitrate concentration stimulated growth of this organism as well as the production of pigments and other metabolites and enzyme activities. In the presence of 100 mM sodium nitrate, different concentrations of …
Invadopodia: Proteolytic Feet Of Cancer Cells, Gi̇zem Bati, Devri̇m Pesen Okvur
Invadopodia: Proteolytic Feet Of Cancer Cells, Gi̇zem Bati, Devri̇m Pesen Okvur
Turkish Journal of Biology
The leading cause of death in cancer patients is metastasis. Invasion is an integral part of metastasis and is carried out by proteolytic structures called invadopodia at the cellular level. In this introductory review, we start by evaluating the definition of invadopodia. While presenting the upstream signaling events involved, we integrate current models on invadopodia. In addition, we discuss the significance of invadopodia in 2D and 3D and in vivo. We finally point out technical challenges and conclude with open questions in the field.
Multidrug Resistance In Chronic Myeloid Leukemia, Mi̇ray Ünlü, Yağmur Ki̇raz, Fatma Necmi̇ye Kaci, Mehmet Ali̇ Özcan, Yusuf Baran
Multidrug Resistance In Chronic Myeloid Leukemia, Mi̇ray Ünlü, Yağmur Ki̇raz, Fatma Necmi̇ye Kaci, Mehmet Ali̇ Özcan, Yusuf Baran
Turkish Journal of Biology
Chronic myeloid leukemia (CML) is characterized by the accumulation of Philadelphia chromosome-positive (Ph+) myeloid cells. Ph+ cells occur via a reciprocal translocation between the long arms of chromosomes 9 and 22 resulting in constitutively active Bcr-abl fusion protein. Tyrosine kinase inhibitors (TKIs) are used against the kinase activity of Bcr-abl fusion protein for the effective treatment of CML. However, the development of drug resistance, directed by different genetic mechanisms, is the major problem of clinical applications of TKIs. These mechanisms include mutations in the TKI binding site of Bcr-abl, overexpression of Bcr-abl, overexpression of ATP binding cassette transporters, aberrant ceramide …