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Full-Text Articles in Life Sciences
Diversity Of Environmental Single-Stranded Dna Phages Revealed By Pcr Amplification Of The Partial Major Capsid Protein, Max S. Hopkins, Shweta Kailasan, Allison Cohen, Simon Roux, Amelia E. Shevenell, Mavis Agbandje-Mckenna, Mya Breitbart
Diversity Of Environmental Single-Stranded Dna Phages Revealed By Pcr Amplification Of The Partial Major Capsid Protein, Max S. Hopkins, Shweta Kailasan, Allison Cohen, Simon Roux, Amelia E. Shevenell, Mavis Agbandje-Mckenna, Mya Breitbart
Marine Science Faculty Publications
The small single-stranded DNA (ssDNA) bacteriophages of the subfamily Gokushovirinae were traditionally perceived as narrowly targeted, niche-specific viruses infecting obligate parasitic bacteria, such as Chlamydia. The advent of metagenomics revealed gokushoviruses to be widespread in global environmental samples. This study expands knowledge of gokushovirus diversity in the environment by developing a degenerate PCR assay to amplify a portion of the major capsid protein (MCP) gene of gokushoviruses. Over 500 amplicons were sequenced from 10 environmental samples (sediments, sewage, seawater and freshwater), revealing the ubiquity and high diversity of this understudied phage group. Residue-level conservation data generated from multiple alignments …
Densovirus Associated With Sea-Star Wasting Disease And Mass Mortality, Ian Hewson, Jason Button, Brent Gudenkauf, Benjamin Miner, Alisa Newton, Mya Breitbart, Elizabeth Fahsbender, Kevin Lafferty
Densovirus Associated With Sea-Star Wasting Disease And Mass Mortality, Ian Hewson, Jason Button, Brent Gudenkauf, Benjamin Miner, Alisa Newton, Mya Breitbart, Elizabeth Fahsbender, Kevin Lafferty
Marine Science Faculty Publications
Sea stars inhabiting the Northeast Pacific Coast have recently experienced an extensive outbreak of wasting disease, leading to their degradation and disappearance from many coastal areas. In this paper, we present evidence that the cause of the disease is transmissible from disease-affected animals to apparently healthy individuals, that the disease-causing agent is a virus-sized microorganism, and that the best candidate viral taxon, the sea star-associated densovirus (SSaDV), is in greater abundance in diseased than in healthy sea stars.