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Open Access. Powered by Scholars. Published by Universities.®

2013

Biology

Turkish Journal of Biology

Enzyme activity

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Production And Characterization Of Uricase From Streptomyces Exfoliatus Ur10 Isolated From Farm Wastes, Magda Aly, Sanaa Tork, Saleh Al-Garni, Reda Allam Jan 2013

Production And Characterization Of Uricase From Streptomyces Exfoliatus Ur10 Isolated From Farm Wastes, Magda Aly, Sanaa Tork, Saleh Al-Garni, Reda Allam

Turkish Journal of Biology

Uricase plays an important role in nitrogen metabolism and can be used medically as a diagnostic reagent. From soil, wastewater, and poultry waste samples collected in Jeddah, 49 bacterial isolates were obtained on either nutrient agar or starch nitrate agar. All the obtained isolates were screened on minimal medium containing 0.5% uric acid for uricase production. The most active bacterium (isolate UR10) produced about 0.5 U/mL of intracellular uricase and was identified as a species belonging to the genus Streptomyces using morphological, physiological, and biochemical characters. By 16S rDNA, it was identified as Streptomyces exfoliatus UR10. Maximum uricase production was …


The Utilization Of Lignocellulosic Wastes For Laccase Production Under Semisolid-State And Submerged Fermentation Conditions, Emre Bi̇rhanli, Özfer Yeşi̇lada Jan 2013

The Utilization Of Lignocellulosic Wastes For Laccase Production Under Semisolid-State And Submerged Fermentation Conditions, Emre Bi̇rhanli, Özfer Yeşi̇lada

Turkish Journal of Biology

The aim of this study was to produce laccase enzymes by using various lignocellulosic wastes (LCWs) under semisolid-state (SsF) and submerged fermentation (SF) conditions. White rot fungi are the best laccase-producing organisms and they can be easily grown on LCW. Thus, in this work, Trametes trogii (Berk.) ATCC 200800 and Trametes versicolor (L.) ATCC 200801, well-known laccase-producing white rot fungi, were used as laccase-producing organisms. According to the literature, some of the LCWs such as sunflower receptacle, apricot seed shell, and bulrush were tested first as substrates for laccase production by these fungi. In SsF, the maximum laccase activities were …


Lignification Response For Rolled Leaves Of Ctenanthe Setosa Under Long-Term Drought Stress, Rabi̇ye Terzi̇, Nesli̇han Saruhan Güler, Ni̇hal Çalişkan, Asi̇m Kadioğlu Jan 2013

Lignification Response For Rolled Leaves Of Ctenanthe Setosa Under Long-Term Drought Stress, Rabi̇ye Terzi̇, Nesli̇han Saruhan Güler, Ni̇hal Çalişkan, Asi̇m Kadioğlu

Turkish Journal of Biology

Leaf rolling is a dehydration avoidance mechanism for plants under drought stress. To understand how it affects the lignification process in response to long-term drought stress in Ctenanthe setosa plants that have the leaf-rolling mechanism, the enzymes in lignification were studied in unrolled leaves as a control and at 2 different leaf rolling indices at days 35 and 47 of the drought period. The results indicated that the activities of phenylalanine ammonia lyase, indole-3-acetic acid oxidase, soluble peroxidase, ionically wall-bound peroxidase, covalently wall-bound peroxidase, and polyphenol oxidase were increased during long-term drought stress. However, nitrate reductase activity was decreased while …