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Articles 1 - 30 of 91
Full-Text Articles in Life Sciences
L-Arginine Protects Against Ethylene Glycol-Induced Gastric Mucosal Damage In Rats: Immunohistochemical And Electron Microscopic Study, Samah Kandeel, Thoryaa El_Deeb, Ehsan Salah, Nafisa El-Bakary, Naglaa Sarhan, Mohamed Balaha, Hiroki Sakai, Tokuma Yanai
L-Arginine Protects Against Ethylene Glycol-Induced Gastric Mucosal Damage In Rats: Immunohistochemical And Electron Microscopic Study, Samah Kandeel, Thoryaa El_Deeb, Ehsan Salah, Nafisa El-Bakary, Naglaa Sarhan, Mohamed Balaha, Hiroki Sakai, Tokuma Yanai
Turkish Journal of Biology
Ethylene glycol is an alcohol widely used in industry as an antifreeze. It affects the normal gastric mucosal defense mechanisms, inducing surface ulcerations. L-arginine is a precursor of nitric oxide that exhibits multiple biological properties; among them is the acceleration of wound healing, especially in the stomach. Caspase-3 immunohistochemistry, and semithin and ultrathin section examinations were used to investigate the possible protective effect of L-arginine on ethylene glycol-induced gastric mucosal damage in rats. Three animal groups were used: group A (control group), group B (received 3.3 mL/kg ethylene glycol for 2 weeks), and group C (received 200 mg/kg of L-arginine …
Effect Of Asiatic And Ursolic Acids On Growth And Virulence Factors Of Uropathogenic Escherichia Coli Strains, Dorota Wojnicz, Dorota Tichaczek-Goska, Marta Kicia
Effect Of Asiatic And Ursolic Acids On Growth And Virulence Factors Of Uropathogenic Escherichia Coli Strains, Dorota Wojnicz, Dorota Tichaczek-Goska, Marta Kicia
Turkish Journal of Biology
This work was aimed at determining the possible antimicrobial potential of asiatic acid (AA) and ursolic acid (UA) against clinical uropathogenic Escherichia coli strains (UPECs). Phylogenetic affiliations of UPECs and virulence-associated gene carriage have been identified. PCR analysis revealed that the studied E. coli strains belong to phylogenetic group B2. All bacterial isolates were grouped into 4 pathotypes. The following were determined: the minimum inhibitory concentrations (MICs) according to CLSI; P-fimbriae synthesis, based on erythrocyte agglutination; curli fibers, by using YESCA agar with congo red; swimming motility, in motility agar; and alpha-hemolysin, on sheeps-blood agar plates. The differences in antigrowth …
Differential Expression Analysis Of Defense-Related Genes Responsive To Tilletia Indica Infection In Wheat, Aradhika Tripathi, Rashmi Aggarwal, Anita Yadav
Differential Expression Analysis Of Defense-Related Genes Responsive To Tilletia Indica Infection In Wheat, Aradhika Tripathi, Rashmi Aggarwal, Anita Yadav
Turkish Journal of Biology
Karnal bunt caused by Tilletia indica is a quarantine disease responsible for qualitative and quantitative losses in wheat. The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method (DDRT-PCR) in susceptible (WL 711) and resistant (HD 29) cultivars of wheat. The DDRT-PCR banding profiles generated by the random primers in combination with oligo-dT primers of resistant and susceptible wheat plants were evaluated at 0 h, 12 h, 24 h, and 48 h after inoculation. Out of 9 sets of primers, …
Activity Of Brassica Napus And Perilla Frutescens Microsomal \Omega-3 Desaturases Expressed In Yeast (Saccharomyces Cerevisiae), Mohammed Abdelreheem, David Hildebrand
Activity Of Brassica Napus And Perilla Frutescens Microsomal \Omega-3 Desaturases Expressed In Yeast (Saccharomyces Cerevisiae), Mohammed Abdelreheem, David Hildebrand
Turkish Journal of Biology
Yeast transformations with Brassica napus and Perilla frutescens \omega-3 desaturases were carried out. Feeding experiments on exogenous 18:2 at 10 mg/L and 100 mg/L for both genes under this investigation showed the same activity levels over the 3 days of the incubation, producing almost the same amount of 18:3. To further investigate the characteristics of B. napus and P. frutescens \omega -3 desaturases, a time-course in vivo activity study was conducted. Both enzymes showed the same behavior over the time course, desaturating 18:2 into 18:3. Moreover, the use of 2 different temperatures for incubation (30 °C and 20 °C) did …
The Effect Of Sooty Mold On Fluorescence And Gas Exchange Properties Of Olive Tree, Sonia A. P. Santos, Conceicao Santos, Sonia Silva, Glória Pinto, Laura M. Torres, Antonio J. A. Nogueira
The Effect Of Sooty Mold On Fluorescence And Gas Exchange Properties Of Olive Tree, Sonia A. P. Santos, Conceicao Santos, Sonia Silva, Glória Pinto, Laura M. Torres, Antonio J. A. Nogueira
Turkish Journal of Biology
Sooty mold is a complex of dark-pigmented fungi that covers leaves and branches of the olive tree (Olea europaea L.). This coverage can bring several consequences for the plant, such as decrease of the photosynthetic rate. Therefore, this study aims to understand the effect of sooty mold on fluorescence and gas exchange of olive leaves. Olive leaves were field-collected and histological, physiological (water content, fluorescence parameters, and carbon dioxide fluxes), and biochemical (osmolality; chlorophyll a, b, and a/b contents; and lipid peroxidation) analyses were performed in healthy leaves and in leaves covered with sooty mold. Histological analysis revealed a complex …
Activation Of The Jak/Stat Pathway In Human Neutrophils By Ndma, Wioletta Ratajczak Wrona, Ewa Jablonska, Marzena Garley, Jakub Jablonski, Piotr Radziwon, Agnieszka Iwaniuk
Activation Of The Jak/Stat Pathway In Human Neutrophils By Ndma, Wioletta Ratajczak Wrona, Ewa Jablonska, Marzena Garley, Jakub Jablonski, Piotr Radziwon, Agnieszka Iwaniuk
Turkish Journal of Biology
The study objective was to assess the role of the JAK/STAT pathway in the induction of nitric oxide (NO) synthesis with the involvement of inducible nitric oxide synthase (iNOS) and in the generation of superoxide anion radical by human neutrophils (PMNs) exposed to N-nitrosodimethylamine (NDMA). Isolated PMNs were incubated in the presence of NDMA. The expression of the study proteins in the cytoplasmic and nuclear fraction was determined by western blotting. Total NO was assayed in cell cultures by Griess reaction. The ability of PMNs to generate superoxide anion radical was assessed using the cytochrome-c reduction test. The current results …
Biopotential Of Verbesina Encelioides (Stem And Leaf Powders) In Silver Nanoparticle Fabrication, Himakshi Bhati-Kushwaha, Chander Parkash Malik
Biopotential Of Verbesina Encelioides (Stem And Leaf Powders) In Silver Nanoparticle Fabrication, Himakshi Bhati-Kushwaha, Chander Parkash Malik
Turkish Journal of Biology
Nanotechnology has opened new opportunities in the field of medical sciences and pharmaceuticals. Commercially, nanoparticles are in great demand in electronics, catalysis, chemistry, energy, and medicine. Today in nanoparticle production green synthesis, using biological material including medicinal plants as a starting material, is in vogue. In the present investigation the powder extract (stems and leaves) of an important medicinal plant, Verbesina encelioides, was used for the biosynthesis of bionanoparticles. The synthesized nanoparticles were screened and characterized by UV-visible spectrophotometer, scanning electron microscopy, X-ray diffraction, and FTIR analyses. The particles were then subjected to antimicrobial assay. Comparative analyses of the antimicrobial …
Genotoxic And Safety Assessment Of 2 Parabens In Somatic Cells Of In Vivo Drosophila Melanogaster, Ari̇f Ayar, Handan Uysal
Genotoxic And Safety Assessment Of 2 Parabens In Somatic Cells Of In Vivo Drosophila Melanogaster, Ari̇f Ayar, Handan Uysal
Turkish Journal of Biology
The objective of this study was to determine the possible genotoxic effects of the para-hydroxybenzoic acid esters (parabens) of methylparaben, propylparaben, and the mixed paraben groups formed by a combination of these, which are used as preservative substances in the food, cosmetic, and drug industries, on Drosophila melanogaster using the wing somatic mutation and recombination test (SMART). According to the data obtained from microscopic analysis, no increase was determined in the single type clone number at various concentrations (100, 150, 200, and 250 mM) of paraben groups in comparison with the control group. In addition, increases corresponding to the concentration …
Genetic Diversity Of Golden Root (Rhodiola Rosea L.) In Northern Norway Based On Recently Developed Ssr Markers, Zsuzsanna György, Erling Fjelldal, Anna Szabó, Paul Eric Aspholm, Andrzej Pedryc
Genetic Diversity Of Golden Root (Rhodiola Rosea L.) In Northern Norway Based On Recently Developed Ssr Markers, Zsuzsanna György, Erling Fjelldal, Anna Szabó, Paul Eric Aspholm, Andrzej Pedryc
Turkish Journal of Biology
Roseroot (Rhodiola rosea L.), an adaptogenic herb, has received increased attention in recent years. The genetic diversity of roseroot was studied with simple sequence repeat (SSR) markers, which have not been widely used so far. Plants were collected in Finnmark County, Norway, from 10 habitats. Eight recently developed microsatellite (SSR) markers were used to assess genetic diversity. However, only 4 SSR markers were informative during this study. The primer pairs for these 4 SSR markers produced 20 fragments with an average of 5 putative alleles per locus. Observed heterozygosity was 1.0 at each locus, whereas expected heterozygosity ranged from 0.60 …
Purification And Characterization Of Trehalase From Seeds Of Chickpea (Cicer Arietinum L.), Maimona Kord, Elhusseiny Youssef, Hanaa Ahmed, Ebtesam Qaid
Purification And Characterization Of Trehalase From Seeds Of Chickpea (Cicer Arietinum L.), Maimona Kord, Elhusseiny Youssef, Hanaa Ahmed, Ebtesam Qaid
Turkish Journal of Biology
In the present study, trehalase was purified and characterized from the seeds of Cicer arietinum L. Giza 1. Crude extract was prepared and purified for electrophoretic homogeneity using ammonium sulfate, chromatography on DEAE-cellulose, CM Sepharose, and Sephadex G-200. The final specific activity was 7 U/mg protein, with 232-fold purification. The purified enzyme exhibited its pH optimum at 5.5. The optimum temperature was 60 °C. The determined K_m value was 3.64 mM trehalose. The enzyme activity was stimulated by 20 mM Mn^{2+}, Ni^{2+}, or Co^{2+}, while it was inhibited by 20 mM Na^+, K^+, Li^+, Ca^{2+}, Zn^{2+}, Cu^{2+}, or Fe^{3+}. Zn^{2+} …
Computational Design Of A Pentapeptide Inhibitor For Fibroblast Growth Factor Receptor 3b (Fgfr3b), Mehmet Ali̇ Öztürk
Computational Design Of A Pentapeptide Inhibitor For Fibroblast Growth Factor Receptor 3b (Fgfr3b), Mehmet Ali̇ Öztürk
Turkish Journal of Biology
Fibroblast growth factor receptor (FGFR) is a cell membrane protein and a member of the tyrosine kinase family. It has extracellular domains that can be activated by ligand binding, followed by receptor dimerization. FGFR3 has 2 isoforms, 3b and 3c. The R248C mutation in FGFR3b leads to ligand independent receptor dimerization and results in different kinds of dermatological diseases such as seborrheic keratoses, acanthosis nigricans, and epidermal nevi. In order to prevent the increased cellular signaling caused by the R248C mutation, a pentapeptide ligand was designed that recognizes the mutation and binds to the receptor dimerization site. Molecular docking and …
The S-Genotyping Of Wild-Grown Apricots Reveals Only Self-Incompatible Accessions In The Erzincan Region Of Turkey, Julia Halasz, Attila Hegedus, Bernadett Szikriszt, Sezai̇ Erci̇şli̇, Emi̇ne Orhan, Hakan Murat Ünlü
The S-Genotyping Of Wild-Grown Apricots Reveals Only Self-Incompatible Accessions In The Erzincan Region Of Turkey, Julia Halasz, Attila Hegedus, Bernadett Szikriszt, Sezai̇ Erci̇şli̇, Emi̇ne Orhan, Hakan Murat Ünlü
Turkish Journal of Biology
The S-genotypes of 63 wild-growing Turkish apricots (Prunus armeniaca L.) were determined by PCR amplification of the S-RNase intron regions and SFB gene in order to characterise their sexual (in)compatibility phenotype. We determined the complete S-genotype of 63 wild-grown apricot accessions that originated in the Erzincan region. Ten previously described and 2 new S-alleles (provisionally labelled S_X and S_Y) were identified in the genotypes. S_2 was the most frequent S-allele in the tested germplasm (occurred in 19 accessions), followed by S_8 (17), S_{19} (16), S_3 (13), S_{12} (11), S_6 (10), and S_7 (10); while S_{9}-, S_{11}-, and S_{13}-alleles were found …
Establishment And Optimization Of Cell Growth In Suspension Culture Of Papaver Bracteatum: A Biotechnology Approach For Thebaine Production, Reza Farjaminezhad, Nasser Zare, Rasool Asghari Zakaria, Manoochehr Farjaminezhad
Establishment And Optimization Of Cell Growth In Suspension Culture Of Papaver Bracteatum: A Biotechnology Approach For Thebaine Production, Reza Farjaminezhad, Nasser Zare, Rasool Asghari Zakaria, Manoochehr Farjaminezhad
Turkish Journal of Biology
Iranian poppy (Papaver bracteatum) is an important medicinal plant that is the main source of the opium alkaloids codeine, morphine, and thebaine. To establish an efficient protocol for cell suspension culture and growth, the effects of different plant growth regulators (2,4-D, NAA, BAP, and kinetin) on callus induction and cell suspension culture of Iranian poppy were evaluated. The maximum percentage of callus induction (86.67%) and fresh weight of callus were obtained in MS medium supplemented with 1 or 2 mg/L 2,4-D, 0.1 or 0.2 mg/L kinetin and 15 mg/L ascorbic acid. The addition of 15 mg/L ascorbic acid to the …
Evaluation Of Phytotoxic And Mutagenic Effects Of Some Cinnamic Acid Derivatives Using The Triticum Test, Alexandra Jitareanu, Silvica Padureanu, Gabriela Tataringa, Cristina Tuchilus, Ursula Stanescu
Evaluation Of Phytotoxic And Mutagenic Effects Of Some Cinnamic Acid Derivatives Using The Triticum Test, Alexandra Jitareanu, Silvica Padureanu, Gabriela Tataringa, Cristina Tuchilus, Ursula Stanescu
Turkish Journal of Biology
Five cinnamic acid derivatives [cinnamic acid, 2,3-dibromo-3-phenyl-propanoic acid, 2,3-dibromo-3-(3-bromophenyl)-propanoic acid, 2,3-dibromo-3-(4-hydroxy-3-methoxyphenyl)-propanoic acid, and 2,3-dibromo-3-(3-bromo-4-hydroxy-5-methoxyphenyl)-propanoic acid] were found to be active against Staphylococcus aureus ATCC 25923, and their minimal bactericidal concentrations were determined (100 µg/mL). The first step in assessing their toxicological potential was the phytotoxicity and genotoxicity evaluation on Triticum aestivum. Wheat seeds were exposed to solutions of the tested compounds (100 µg/mL) for 24 and 48 h. The development of roots and seedlings, germination percentage, mitotic index, chromosomal aberrations, and total polyphenol content were analyzed. The substances caused in most experimental cases a slight inhibition in the growth of …
Micropropagation And Antimicrobial Activity Of Curcuma Aromatica Salisb., A Threatened Aromatic Medicinal Plant, Shamima Akhtar Sharmin, Mohammad Jahangir Alam, Md. Mominul Islam Sheikh, Rashed Zaman, Muhammad Khalekuzzaman, Sanjoy Chandra Mondal, Mohammad Anwarul Haque, Mohammad Firoz Alam, Iftekhar Alam
Micropropagation And Antimicrobial Activity Of Curcuma Aromatica Salisb., A Threatened Aromatic Medicinal Plant, Shamima Akhtar Sharmin, Mohammad Jahangir Alam, Md. Mominul Islam Sheikh, Rashed Zaman, Muhammad Khalekuzzaman, Sanjoy Chandra Mondal, Mohammad Anwarul Haque, Mohammad Firoz Alam, Iftekhar Alam
Turkish Journal of Biology
A rapid and improved micropropagation protocol was developed for Curcuma aromatica, a threatened aromatic medicinal plant, using rhizome sprout as the explant. Stepwise optimization of different plant growth regulators, carbon sources, and basal media was adopted to establish an efficient micropropagation protocol. When cytokinins, such as benzyl amino purine (BAP) or 6-(\alpha,\alpha-dimethylallylamino)-purine (2iP), were used either singly or in combination with naphthalene acetic acid (NAA) for shoot induction and multiplication, a single use of BAP was the most effective. As a carbon source, 3% (w/v) sucrose exhibits the greatest promotive effect on shoot initiation and proliferation compared with other carbon …
Assessment Of Genetic Diversity In Naturally Growing 29 Trifolium L. Taxa From Bolu Province Using Rapd And Ssr Markers, Emel Uslu, Gözde Damla Ertuğrul, Mehmet Teki̇n Babaç
Assessment Of Genetic Diversity In Naturally Growing 29 Trifolium L. Taxa From Bolu Province Using Rapd And Ssr Markers, Emel Uslu, Gözde Damla Ertuğrul, Mehmet Teki̇n Babaç
Turkish Journal of Biology
Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300-3500 bp with RAPD and 125-3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccards coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR …
Antimicrobial Susceptibility Patterns Of Environmental And Hospital Isolations Of Enterococci In Aydın, Erman Oryaşin, Haci Hali̇l Biyik, Gamze Başbülbül, Bülent Bozdoğan
Antimicrobial Susceptibility Patterns Of Environmental And Hospital Isolations Of Enterococci In Aydın, Erman Oryaşin, Haci Hali̇l Biyik, Gamze Başbülbül, Bülent Bozdoğan
Turkish Journal of Biology
The aim of the present study was to evaluate antimicrobial susceptibilities of enterococci isolated from water, soil, waste, and food samples to determine resistance mechanisms and their transferability, and to test clonality of these environmental enterococci. The results were compared with the susceptibility patterns of hospital enterococci reported in a previous study. Samples from 9 environmental sources were cultured, and species were identified using 16S rRNA sequencing. Antimicrobial susceptibilities were determined by the agar dilution method. The minimum inhibitory concentrations were tested for vancomycin, tetracycline, teicoplanin, ampicillin, gentamicin, erythromycin, rifampicin, clindamycin, chloramphenicol, and ciprofloxacin. Resistance genes and transposons were determined …
Production And Characterization Of Uricase From Streptomyces Exfoliatus Ur10 Isolated From Farm Wastes, Magda Aly, Sanaa Tork, Saleh Al-Garni, Reda Allam
Production And Characterization Of Uricase From Streptomyces Exfoliatus Ur10 Isolated From Farm Wastes, Magda Aly, Sanaa Tork, Saleh Al-Garni, Reda Allam
Turkish Journal of Biology
Uricase plays an important role in nitrogen metabolism and can be used medically as a diagnostic reagent. From soil, wastewater, and poultry waste samples collected in Jeddah, 49 bacterial isolates were obtained on either nutrient agar or starch nitrate agar. All the obtained isolates were screened on minimal medium containing 0.5% uric acid for uricase production. The most active bacterium (isolate UR10) produced about 0.5 U/mL of intracellular uricase and was identified as a species belonging to the genus Streptomyces using morphological, physiological, and biochemical characters. By 16S rDNA, it was identified as Streptomyces exfoliatus UR10. Maximum uricase production was …
Silver Nanoparticles: Cytotoxic, Apoptotic, And Necrotic Effects On Mcf-7 Cells, Hakan Çi̇ftçi̇, Mustafa Türk, Uğur Tamer, Si̇yami̇ Karahan, Yusuf Menemen
Silver Nanoparticles: Cytotoxic, Apoptotic, And Necrotic Effects On Mcf-7 Cells, Hakan Çi̇ftçi̇, Mustafa Türk, Uğur Tamer, Si̇yami̇ Karahan, Yusuf Menemen
Turkish Journal of Biology
The present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. The size of AgNPs was measured by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering. Zeta potential of AgNPs was determined by laser Doppler microelectrophoresis. After exposing MCF-7 cells to AgNPs for 24 h, cytotoxicity was measured by WST-1 assay. Apoptosis and necrosis in MCF-7 cells were detected by Annexin-V-FLUOS immunostaining and double staining of Hoechst dye with propidium iodide. …
In Vitro And In Silico Characterization Of Solanum Lycopersicum Wound-Inducible Proteinase Inhibitor-Ii Gene, Faiza Munir, Syed Muhammad Saqlan Naqvi, Tariq Mahmood
In Vitro And In Silico Characterization Of Solanum Lycopersicum Wound-Inducible Proteinase Inhibitor-Ii Gene, Faiza Munir, Syed Muhammad Saqlan Naqvi, Tariq Mahmood
Turkish Journal of Biology
Plant proteinase inhibitors (PIs) are antimetabolic defensive proteins conferring resistance in plants against a variety of competing organisms such as bacteria, viruses, fungi, attacking nematodes, and insects. In the fields of plant biochemistry and molecular genetics research, tremendous success has been achieved in generating transgenic crops that have defensive approaches against biotic challenges. In this study, in vitro and in silico analysis was carried out for a wound-inducible PI-II gene isolated from 4 selected varieties (Roma, Nagina, Moneymaker, and Rio Grande) of Solanum lycopersicum L. Around 684 bp of PI-II gene was amplified, sequenced, translated, modeled to protein structure, and …
The Effect Of 3-Methylcholanthrene And Butylated Hydroxytoluene On Glycogen Levels Of Liver, Muscle, Testis, And Tumor Tissues Of Rats, Fi̇kri̇ye Polat, Egemen Dere, Eylem Gül, İzzet Yelkuvan, Öztürk Özdemi̇r, Günsel Bi̇ngöl
The Effect Of 3-Methylcholanthrene And Butylated Hydroxytoluene On Glycogen Levels Of Liver, Muscle, Testis, And Tumor Tissues Of Rats, Fi̇kri̇ye Polat, Egemen Dere, Eylem Gül, İzzet Yelkuvan, Öztürk Özdemi̇r, Günsel Bi̇ngöl
Turkish Journal of Biology
This study examined the effects of separate and combined applications of 3-methylcholanthrene, a polycyclic aromatic hydrocarbon and potent carcinogenic agent, and butylated hydroxytoluene, the antioxidant food additive, on the glycogen levels of liver, muscle, testis, and tumor tissues in rats. Adult male Wistar albino rats weighing 100-110 g at 8 weeks of age were used in this study. This study consisted of a control group (n = 9) and 3 different experiment groups in which rats were chronically treated with 3-methylcholanthrene (n = 9) or butylated hydroxytoluene (n = 11) or a combination of these agents (n = 14). Rats …
Salicylic Acid Delays Leaf Rolling By Inducing Antioxidant Enzymes And Modulating Osmoprotectant Content In Ctenanthe Setosa Under Osmotic Stress, Mehmet Demi̇ralay, Aykut Sağlam, Asi̇m Kadioğlu
Salicylic Acid Delays Leaf Rolling By Inducing Antioxidant Enzymes And Modulating Osmoprotectant Content In Ctenanthe Setosa Under Osmotic Stress, Mehmet Demi̇ralay, Aykut Sağlam, Asi̇m Kadioğlu
Turkish Journal of Biology
The effects of salicylic acid (SA) on leaf rolling under osmotic stress in Ctenanthe setosa (Roscoe) Eichler were evaluated. The leaves were pretreated with SA, paclobutrazol (PAC), and antioxidant enzyme inhibitors for 15 h and then kept in polyethylene glycol (PEG) for 4 h to promote osmotic stress. The degree of leaf rolling was measured every hour during the 4-h PEG treatment. Antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase) and NADPH oxidase (NOX) activities, endogenous SA contents, hydrogen peroxide, proline, and soluble sugar were determined immediately after PEG treatment. SA treatment retarded leaf rolling and induced antioxidant …
Using Seedlessness-Related Molecular Markers In Grapevine Breeding For Seedlessness Via Marker-Assisted Selection Into Muscat Of Hamburg × Sultani Progeny, Murat Akkurt, Ati̇lla Çakir, Mi̇na Shi̇dfar, Fi̇li̇z Mutaf, Gökhan Söylemezoğlu
Using Seedlessness-Related Molecular Markers In Grapevine Breeding For Seedlessness Via Marker-Assisted Selection Into Muscat Of Hamburg × Sultani Progeny, Murat Akkurt, Ati̇lla Çakir, Mi̇na Shi̇dfar, Fi̇li̇z Mutaf, Gökhan Söylemezoğlu
Turkish Journal of Biology
The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) × Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as …
Cytotoxic Activities Of Some Pseudomonas Aeruginosa Isolates: Possible Mechanisms And Approaches For Inhibition, Ghadir Saeed El-Housseiny, Mohammad Mabrouk Aboulwafa, Nadia Abdelhaleim Hassouna
Cytotoxic Activities Of Some Pseudomonas Aeruginosa Isolates: Possible Mechanisms And Approaches For Inhibition, Ghadir Saeed El-Housseiny, Mohammad Mabrouk Aboulwafa, Nadia Abdelhaleim Hassouna
Turkish Journal of Biology
The cytotoxicity of 6 highly cytotoxic Pseudomonas aeruginosa isolates was tested against Vero cells. Cytotoxicity was found to be cell-associated, high cytotoxicity was observed at a multiplicity of infection of 200:1, and this activity gradually decreased as the bacterial count decreased. This cytotoxicity caused rounding and detachment of the Vero monolayer as early as 1 h after infection. Moreover, viable metabolically active bacteria were necessary for Vero cell killing. These results imply that one or more of the type III secretion system effector proteins (ExoU, ExoS, ExoT, or ExoY) were responsible for the observed cytotoxic effects. This cytotoxicity was affected …
Fatty Acid Composition Of Root And Shoot Samples Of Some Astragalus L. (Fabaceae) Taxa Growing In The East And Southeast Of Turkey, Cumali̇ Keski̇n, Semra Kaçar
Fatty Acid Composition Of Root And Shoot Samples Of Some Astragalus L. (Fabaceae) Taxa Growing In The East And Southeast Of Turkey, Cumali̇ Keski̇n, Semra Kaçar
Turkish Journal of Biology
The fatty acid compositions of root and shoot samples of some Astragalus L. (Fabaceae) taxa [A. gossypinus Fisch., A. amblolepis Fisch., A. edmondi (Kuntze) Sheld., A. tigridis Boiss., A. aleppicus Boiss., A. suberosus Banks & Sol. subsp. suberosus Banks & Sol., A. gummifer Labill., A. diphtherites Fenzl var. diphtherites Fenzl, and A. gymnalopecias Rech.f.] growing in the east and southeast of Turkey were determined by gas chromatography. Astragalus species contained palmitic (C 16:0) (20.13%-53.8%), linoleic (C 18:2\omega-6) (13.25%-41.06%), oleic (C 18:1\omega-9) (5.78%-25.7%), linolenic (C 18:3\omega-3) (6.1%-22.89%), and stearic acid (C 18:0) (5.16%-13.1%) in the roots and linolenic (8.9%-51.42%), palmitic (20.16%-44.88%), …
Antimicrobial, Antioxidant, And Synergistic Properties Of Two Nutraceutical Plants: Terminalia Catappa L. And Colocasia Esculenta L., Sumitra Chanda, Kalpna Rakholiya, Komal Dholakia, Yogesh Baravalia
Antimicrobial, Antioxidant, And Synergistic Properties Of Two Nutraceutical Plants: Terminalia Catappa L. And Colocasia Esculenta L., Sumitra Chanda, Kalpna Rakholiya, Komal Dholakia, Yogesh Baravalia
Turkish Journal of Biology
Antibiotics have been effective in treating infectious diseases, but resistance to these drugs has led to the emergence of new and reemergence of old infectious diseases. Using a combination of plant extracts and antibiotics is one way of combating these multidrug-resistant microorganisms. The aim of this investigation was to evaluate the antimicrobial and antioxidant properties of 2 nutraceutical plants: Terminalia catappa and Colocasia esculenta. The antimicrobial activity of the plants was evaluated against 25 microorganisms by agar well diffusion method. C. esculenta did not show any antimicrobial activity against bacteria and fungi. Therefore, only methanol extract of T. catappa was …
Antioxidant Activity Of In Vitro Propagated Stevia Rebaudiana Bertoni Plants Of Different Origins, Ely Zayova, Ira Stancheva, Maria Geneva, Maria Petrova, Lyudmila Dimitrova
Antioxidant Activity Of In Vitro Propagated Stevia Rebaudiana Bertoni Plants Of Different Origins, Ely Zayova, Ira Stancheva, Maria Geneva, Maria Petrova, Lyudmila Dimitrova
Turkish Journal of Biology
An efficient in vitro protocol for propagation of Stevia rebaudiana Bertoni is described. Multiple shoots were induced in vitro from shoot tip and nodal segments on Murashige and Skoog medium containing 6-benzylaminopurine, zeatin, or thidiazuron alone and in combination with naphthalene acetic acid or indole-3-acetic acid. A high frequency of shoot induction as well as maximum number of shoots per shoot tip explant was observed on Murashige and Skoog medium supplemented with 6-benzylaminopurine (1.0 mg L^{-1}) alone and combined with indole-3-acetic acid (0.1 mg L^{-1}). For root induction, in vitro shoots were transferred to rooting media containing naphthalene acetic acid, …
Investigation Of The Genotoxic And Antigenotoxic Properties Of Essential Oils Obtained From Two Origanum Species By Drosophila Wing Smart Assay, Eşref Demi̇r, Bülent Kaya, Ricard Marcos, Serap Kocaoğlu Cenkci̇, Hüseyi̇n Çeti̇n
Investigation Of The Genotoxic And Antigenotoxic Properties Of Essential Oils Obtained From Two Origanum Species By Drosophila Wing Smart Assay, Eşref Demi̇r, Bülent Kaya, Ricard Marcos, Serap Kocaoğlu Cenkci̇, Hüseyi̇n Çeti̇n
Turkish Journal of Biology
In this study, essential oils extracted from 2 species of oregano, Origanum onites L. and Origanum minutiflorum O.Schwarz & P.H.Davis, were assayed for genotoxicity using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. Results indicated that there is no significant genotoxic effect of the 2 oils tested. In addition, the antigenotoxic activities of these oils against potassium dichromate (K_{2}Cr_{2}O_{7}) and cobalt chloride (CoCl_{2}) were tested. Results indicated that essential oil cotreatments were able to abolish the genotoxic effects induced by the 2 metal compounds. It is suggested that the observed effects can be linked to the antioxidant properties …
The Effects Of Zinc Chloride During Early Embryonic Development In Zebrafish (Brachydanio Rerio), Melek Küçükoğlu, Uğur Seçi̇l Bi̇nokay, Ayper Boğa Pekmezekmek
The Effects Of Zinc Chloride During Early Embryonic Development In Zebrafish (Brachydanio Rerio), Melek Küçükoğlu, Uğur Seçi̇l Bi̇nokay, Ayper Boğa Pekmezekmek
Turkish Journal of Biology
This study investigated the developmental toxicity of zinc chloride (ZnCl_{2}) in zebrafish embryos (Brachydanio rerio, Cyprinidae, Cypriniformes). Zebrafish embryos were exposed to 5 different concentrations of ZnCl_{2}, from the blastula stage, for 15 days under static renewal test conditions. As a result, the corresponding median lethal concentration (LC_{50}) value determined for ZnCl_2 exposure was 1.36 mg/L (0.65 mg/L as a lone Zn^{2+} ion). At 1.0 mg/L ZnCl_{2}, the exposed group's hatching began at 7 days instead of at 4 days, and most of the embryos died in the chorion without hatching at 11 and 12 days. Developmental deformities such as …
Evaluation Of Anti-Plasmodium Berghei Activity Of Crude And Column Fractions Of Extracts From Withania Somnifera, Zerihun Teklemariam Dame, Beyene Petros, Yalemtsehay Mekonnen
Evaluation Of Anti-Plasmodium Berghei Activity Of Crude And Column Fractions Of Extracts From Withania Somnifera, Zerihun Teklemariam Dame, Beyene Petros, Yalemtsehay Mekonnen
Turkish Journal of Biology
Different crude methanolic and chloroform extracts of Withania somnifera leaves, and column fractions of the methanolic extract, were tested in vivo for antimalarial activity on Swiss albino male mice. Each mouse in the study was infected intraperitoneally with 0.2 mL of blood containing 10^6-10^7 infected erythrocytes taken from mice previously infected with chloroquine-sensitive Plasmodium berghei. The extracts were given to the infected mice intraperitoneally starting from 3 h following inoculation. Antimalarial activity was evaluated by taking blood smears on day 4. The results revealed that column fractions of methanolic extract obtained from leaves of Withania somnifera showed significantly improved suppression …