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High-Throughput Imaging Of Neuronal Activity In Caenorhabditis Elegans, Dirk Albrecht, Johannes Larsch, Donovan Ventimiglia, Cornelia Bargmann
High-Throughput Imaging Of Neuronal Activity In Caenorhabditis Elegans, Dirk Albrecht, Johannes Larsch, Donovan Ventimiglia, Cornelia Bargmann
Dirk R. Albrecht
Neuronal responses to sensory inputs can vary based on genotype, development, experience, or stochastic factors. Existing neuronal recording techniques examine a single animal at a time, limiting understanding of the variability and range of potential responses. To scale up neuronal recordings, we here describe a system for simultaneous wide-field imaging of neuronal calcium activity from at least 20 Caenorhabditis elegans animals under precise microfluidic chemical stimulation. This increased experimental throughput was used to perform a systematic characterization of chemosensory neuron responses to multiple odors, odor concentrations, and temporal patterns, as well as responses to pharmacological manipulation. The system allowed recordings …