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Full-Text Articles in Life Sciences
High-Throughput Imaging Of Neuronal Activity In Caenorhabditis Elegans, Dirk Albrecht, Johannes Larsch, Donovan Ventimiglia, Cornelia Bargmann
High-Throughput Imaging Of Neuronal Activity In Caenorhabditis Elegans, Dirk Albrecht, Johannes Larsch, Donovan Ventimiglia, Cornelia Bargmann
Dirk R. Albrecht
Neuronal responses to sensory inputs can vary based on genotype, development, experience, or stochastic factors. Existing neuronal recording techniques examine a single animal at a time, limiting understanding of the variability and range of potential responses. To scale up neuronal recordings, we here describe a system for simultaneous wide-field imaging of neuronal calcium activity from at least 20 Caenorhabditis elegans animals under precise microfluidic chemical stimulation. This increased experimental throughput was used to perform a systematic characterization of chemosensory neuron responses to multiple odors, odor concentrations, and temporal patterns, as well as responses to pharmacological manipulation. The system allowed recordings …
Microfluidics-Integrated Time-Lapse Imaging For Analysis Of Cellular Dynamics, Dirk Albrecht, Gregory Underhill, Joshua Resnikoff, Avital Mendelson, Sangeeta Bhatia, Jagesh Shah
Microfluidics-Integrated Time-Lapse Imaging For Analysis Of Cellular Dynamics, Dirk Albrecht, Gregory Underhill, Joshua Resnikoff, Avital Mendelson, Sangeeta Bhatia, Jagesh Shah
Dirk R. Albrecht
An understanding of the mechanisms regulating cellular responses has recently been augmented by innovations enabling the observation of phenotypes at high spatio-temporal resolution. Technologies such as microfluidics have sought to expand the throughput of these methods, although assimilation with advanced imaging strategies has been limited. Here, we describe the pairing of high resolution time-lapse imaging with microfluidic multiplexing for the analysis of cellular dynamics, utilizing a design selected for facile fabrication and operation, and integration with microscopy instrumentation. This modular, medium-throughput platform enables the long-term imaging of living cells at high numerical aperture (via oil immersion) by using a conserved …
Photo- And Electropatterning Of Hydrogel-Encapsulated Living Cell Arrays, Dirk Albrecht, Valerie Tsang, Robert Sah, Sangeeta Bhatia
Photo- And Electropatterning Of Hydrogel-Encapsulated Living Cell Arrays, Dirk Albrecht, Valerie Tsang, Robert Sah, Sangeeta Bhatia
Dirk R. Albrecht
Living cells have the potential to serve as sensors, naturally integrating the response to stimuli to generate predictions about cell fate (e.g., differentiation, migration, proliferation, apoptosis). Miniaturized arrays of living cells further offer the capability to interrogate many cells in parallel and thereby enable high-throughput and/or combinatorial assays. However, the interface between living cells and synthetic chip platforms is a critical one wherein the cellular phenotype must be preserved to generate useful signals. While some cell types retain tissue-specific features on a flat (2-D) surface, it has become increasingly apparent that a 3-D physical environment will be required for others. …