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Dual Role Of The Metalloprotease Ftsh In Biogenesis Of The Drrab Drug Transporter, Wen Li, Divya Kishore Rao, Parjit Kaur

Biology Faculty Publications

This study provides the first direct evidence for the dual role of the metalloprotease FtsH in membrane protein biogenesis. Using the physiological substrate DrrAB, it is shown that FtsH is not only responsible for proteolysis of unassembled DrrB protein but it also plays a much broader role in biogenesis of the DrrAB complex. Previous studies showed that the stable expression of DrrB in the membrane depends on simultaneous expression of DrrA. Here we show that DrrB is proteolyzed by FtsH when it is expressed alone. Moreover, DrrA and DrrB proteins expressed together in a temp-sensitive ftsH mutant strain of E. …