Life Sciences Commons^™

Take Pride In America Phase Iii: Quarterly Progress Report, Period Covering April 25, 2012-July 24, 2012, Margaret N. Rees

Anti-littering Programs

• The GIS database continued to be populated with data.
• Hector’s Helpers added an additional youth group.
• The new website launched and social media efforts began.
• The pilot testing of the litter monitoring program continued.

The University of Nevada Las Vegas Public Lands Institute (PLI) designed the Take Pride in America (TPIA) Phase III work plan based on the strategic plan developed by the Southern Nevada Agency Partnership (SNAP) anti-litter and desert dumping team during Phase II of the program.

Walking Box Ranch Planning And Design Quarterly Progress Report: Period Ending July 10, 2012, Margaret N. Rees

Walking Box Ranch

• BLM informed UNLV that after investigating a concession contract as the appropriate BLM document for the future operating partner, they have concluded that an operating agreement is the preferred document and they are proceeding to complete such an agreement as soon as possible.
• Following a visit to the ranch with BLM, UNLV has completed temporary repairs to ranch house windows, which should mitigate deterioration of the ranch house from water
• UNLV participated in a one-half day meeting with BLM to review Condit’s Extended Content Package #1. As a result of the lack of progress by Condit, UNLV was requested to …

Assessment Of A Microbicide Candidate Among A Diverse Cohort Of Urban Southern Us Women And Their Male Sexual Partners, Paula M. Frew, Kimberly Parker, Takeia Horton, Brooke Hixson, Lisa Flowers, Frances Priddy, Lisa Grohskopf, Christine Mauck, Kimberly Workowski

Environmental & Occupational Health Faculty Publications

Background: This mixed methods study reports on product acceptance from a Phase I clinical trial of a candidate non-nucleoside reverse transcriptase inhibitor (NNRTI) vaginal microbicide product (UC781). The product was evaluated in the context of a Phase I clinical trial in an area characterized by high HIV prevalence among minority women. The findings will inform the development of an acceptable microbicide that will address the needs of diverse women and their partners. Methods: This is a mixed methods study of 34 racially and ethnically diverse female participants and 10 male partners in Atlanta, Georgia. Chi-square tests for marginal homogeneity and …

The Human Phosphotyrosine Signaling Network: Evolution And Hotspots Of Hijacking In Cancer., Lei Li, Chabane Tibiche, Cong Fu, Tomonori Kaneko, Michael F. Moran, Martin Schiller, Shawn Shun-Cheng Li, Edwin Wang

Life Sciences Faculty Research

Phosphotyrosine (pTyr) signaling, which plays a central role in cell-cell and cell-environment interactions, has been considered to be an evolutionary innovation in multicellular metazoans. However, neither the emergence nor the evolution of the human pTyr signaling system is currently understood. Tyrosine kinase (TK) circuits, each of which consists of a TK writer, a kinase substrate, and a related reader, such as Src homology (SH) 2 domains and pTyr-binding (PTB) domains, comprise the core machinery of the pTyr signaling network. In this study, we analyzed the evolutionary trajectories of 583 literature-derived and 50,000 computationally predicted human TK circuits in 19 representative …

Association Of Vitamin D Deficiency With Hypertension In Uninsured Women, Sreenivasa R. Chandana, Lakshmi P. Kocharla, Susan S. Harris, Radhika R. Kakarala

Journal of Health Disparities Research and Practice

Vitamin D deficiency is an epidemic in the United States. Uninsured women are at high risk due to a lower intake of vitamin D and limited sun exposure. We examined the association between vitamin D deficiency and hypertension in 96 uninsured women at a County Free Medical Clinic in urban Michigan. Questionnaires were used to obtain information about demographics, medical history including hypertension, and dietary habits. Measurements including blood pressure and serum 25(OH)D level were also collected. Prevalence of hypertension was higher in subjects with 25(OH)D less than 50nmol/l compared with others (85% vs. 27.3%, p = 0.014). For every …

Immediate Effects Of High Intensity Training In Children With Cerebral Palsy Gmfcs Levels I-Iii: A Pilot Study, Andrea Blahovec, Andrea Kuiken, Jillian Mears, Heather Riggins

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: Cerebral Palsy is one of the most common causes of motor disability in the U.S., but there is still a lack of consensus for best intervention strategies to improve function and gait efficiency.

Objective: Determine if ambulatory children with CP, exposed to a brief, high intensity training session, will: 1) experience changes in temporal-spatial gait characteristics 2) demonstrate increased gait speed and 3) demonstrate improved gait kinematics.

Design: Five participants walked at preferred and fast speeds over an instrumented walkway followed by a 15-minute intervention. After a short rest, post-intervention walking was completed.

Results: Ten dependent variables were extracted …

Active Recovery And Electro-Muscular Stimulation On Delayed Onset Muscle Soreness After Endurance Running: A Randomized Clinical Trial, Amanda J. Gramly, Kitrick C. Rhodes, Andrea F. Smith

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background/Purpose: Current research on strategies to decrease delayed onset muscle soreness (DOMS) has focused on the effects of active and passive recovery on athletic performance across various sports, but there is little evidence regarding these recoveries at reducing DOMS induced by aerobic activities, such as running. In addition, there is limited research regarding the efficacy of recovery using electro-muscular stimulation (EMS). Therefore, the purpose of this study was to investigate the effects of active and EMS recovery in decreasing DOMS. Subjects: Forty-eight healthy subjects (25 males and 23 females) between the ages of 20 and 40 (25.1 ± 2.9) participated. …

Exploring The Seed Bank Dynamics Of Red Brome: Longevity, Density, And Relationship To Fire, Benjamin S. Jurand

UNLV Theses, Dissertations, Professional Papers, and Capstones

This research explores several untested aspects of the seed bank characteristics of red brome (Bromus rubens), an invasive annual grass in southwestern United States arid lands. Red brome is a formidable competitor to native plant species, both annual and perennial alike, and produces many seeds that germinate easily. The stalks of red brome contribute continuous-cover fuel loads that facilitate wildfires destructive to mature native Mojave Desert plant communities. This makes it a priority species for land managers, particularly when dealing with recovery after fire.

This project addressed questions related to the longevity of red brome seeds in soil seed banks …

Investigating Three Decades Of Vegetation Change In A Mojave Desert Mountain Range, Chris Lee Roberts

UNLV Theses, Dissertations, Professional Papers, and Capstones

This project is a vegetation change study spanning 29 years in the Newberry Mountains of Southern Nevada. Long term monitoring data are crucial for understanding the effects of climate change on vegetation dynamics. Successful management intervention in vegetation change will require identification of early indicator plant species and their responses to climatic cues. This project is one of the oldest comparisons of resurveyed Mojave vegetation community plots with repeatable methodology and the longest survey interval reported for the southeastern Mojave Desert. 103 plots were relocated and resurveyed based on data methods in Jim Holland's thesis titled "A Vegetative Analysis of …

Exercise-Induced Neuroprotection In A Hemiparkinsonian 6-Hydroxydopamine Rat Model, Merrill Russen Landers

UNLV Theses, Dissertations, Professional Papers, and Capstones

Outside of finding a cure, one of the preeminent goals of research in Parkinson's disease (PD) is finding a neuroprotective treatment that when applied prior to the onset of the disease will decrease the risk and severity of the subsequent disease. One such treatment that has potential as a neuroprotective agent in PD is exercise. Several studies have found forced exercise to be protective of Parkinson's disease in adult rodent models; however, few of these studies have used a design wherein voluntary exercise was evaluated. Moreover, no study has used a true neuroprotective design in which exercise was applied prior …

Nutritional Management Of The Extremely Low Birth Weight Infant: An Evidence Based Clinical Practice Guideline, Dana J. Lunde

UNLV Theses, Dissertations, Professional Papers, and Capstones

Premature birth has risen steadily over the last decade resulting in an increased number of infants needing neonatal intensive care. The demand for neonatal intensive care has lead to many improvements in the delivery of such care with an increase in the survival of the smallest and most premature infants. However, most of these infants are being discharged from neonatal intensive care units (NICU) with severe extrauterine growth restriction (EUGR).

Extremely low birth weight (ELBW) infants born at 23-27 weeks gestation and who have a weight of less than 1,000 grams are at the highest risk for EUGR because of …

Prehistoric Foraging Strategies In The Piute Valley Of Southern Nevada, Christopher Alan Brosman

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Piute Valley of Southern Nevada is an incredibly diverse but arid zone in the eastern portion of the Mojave Desert. Most of this diversity can be attributed to the elevation shifts ranging from the Colorado River basin to the peaks of the surrounding mountain ranges. These peaks and valleys provide a multitude of resource zones in which prehistoric hunter-gatherers could provision themselves throughout the year. For this thesis I have used archaeological survey, paleo-climate models, life-sciences data and ethnographic research to perform an in-depth land use analysis of prehistoric forager adaptations to this challenging but life-sustaining environment.

Recent investigations …

You Should Know Jack: A Qualitative Study Of The Jack Lalanne Show (1951-~1965), Robert Cochrane

UNLV Theses, Dissertations, Professional Papers, and Capstones

Jack LaLanne hosted the first and longest running fitness program in United States broadcast history from 1951 through 1985. Since LaLanne's rise as a broadcasting celebrity, the health and fitness industry has grown from a small, somewhat-maligned field into a multi-billion dollar per year economy of its own. As LaLanne reached iconic status through his show, his name became synonymous with good health and nutrition, but the messages of his show went far beyond simple exercises. He used religious, patriotic, and biomedical messages to get his points across. In addition, he was a showman who sang to his audience, used …

The Relationship Between Self-Efficacy And Fluid,And Dietary Compliance In Hemodialysis Patients, Ansy Paul John

UNLV Theses, Dissertations, Professional Papers, and Capstones

Patients with end-stage renal disease (ESRD) who receive hemodialysis often fail to follow a prescribed diet and fluid regimen, which undermines the effectiveness of care and leads to unpredictable disease progression and greater likelihood of complications. Non-adherence to dietary and fluid limitations compromises the outcomes of patients receiving hemodialysis. Noncompliance can lead to detrimental long-term outcomes including deterioration of the cardiovascular system, heart failure, hypertension, and pulmonary edema as well as short-term problems such as edema, itching, bone pain, and breathlessness.

Many studies have demonstrated that self-efficacy strategies are associated with improved dietary and fluid compliance among individuals receiving hemodialysis. …

Exercise And Nutrition Survey Of Nevada Air National Guard Members, Matthew Antonio Demattei

UNLV Theses, Dissertations, Professional Papers, and Capstones

One hundred seventy-three Nevada Air National Guard members voluntarily participated in this exercise science and nutrition survey-based study. The survey consisted of ten closed-ended questions; the intent of the study was to determine whether the participants were willing to attend exercise science or nutrition related classes, or if the participants were willing to instruct exercise science or nutrition classes if they possessed a degree in either field. Forty-six females, and one hundred twenty-seven males participated in this study; one hundred thirty-six of the respondents were enlisted, and thirty-seven of the respondents were officers. Participants were demographically separated by age groups …

Natural And Constructed Wetlands For Ecosystem And Engineering Services In The Arid And Semi-Arid Regions, Achyut Raj Adhikari

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Las Vegas Wash (LVW) has undergone significant wetlands degradation and soil erosion over the past thirty years due to increasing flow resulting from urbanization and large rainfall events in the Las Vegas Valley Watershed. The increased flow and associated pollution load in the LVW and its adverse impact in Lake Mead have alerted stakeholders to pay a greater attention to explore alternative measures for rehabilitation of wetland ecosystems. This dissertation, using the case of changes in LVW, analyzes and describes ecological and engineering services provided by wetlands in arid and semi-arid regions and provides a knowledge base that can …

The Acute Effect Of Endurance Exercise On Lipoproteins Measured By Nuclear Magnetic Resonance (Nmr) In Healthy Men, Hanaa Adnan Shaheen

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: Cardiovascular disease (CVD) represents a major cause of death in the United States, with abnormal levels of blood lipids and physical inactivity considered as major modifiable risk factors. The conventional lipid profile has been used to assess for CVD risk by directly measuring the concentrations of blood lipids. However, lipoprotein particle size and number obtained from a novel method, Nuclear Magnetic Resonance (NMR) may also asses for CVD risk with greater sensitivity. Exercise and increased physical activity has been shown to produce favorable effects on blood lipids and consequently reduce CVD risk. To understand this effect, it is important …

Reliability And Validity Of The Body Caliper To Evaluate Body Composition, Gregory L. Stalker

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Body Caliper is a relatively new model of skin–fold caliper (approximately 13 years old) whose reliability and validity has yet to be investigated on an adult population. Therefore, the purpose of this study was to investigate the reliability and validity of the Body Caliper as an instrument and method for evaluating body composition in adults. One hundred adults (62 men, 38 women) aged 18 to 60 years participated in this cross–sectional study. Skin–fold measurements were taken with the Body Caliper using the YMCA protocol, and body density was calculated by the Jackson & Pollock sum–of–3 equations for men and …

Community Gardening: Benefits Focused Strategies, Joseph Zaanan Tellschow

UNLV Theses, Dissertations, Professional Papers, and Capstones

Throughout history humans have had varying degrees of interaction with nature and natural environments. This interaction has shaped our species in almost every way possible from our physical traits to our behaviors and places we have chosen to occupy. Currently, humans have developed such overwhelming mastery of the planet that it is easy for many humans to feel disconnected from nature. This separation could have detrimental effects on various aspects of human well-being including physical and mental health. To understand these effects, it is important to evaluate the benefits that humans receive from exposure to nature and interaction with natural …

Nevada Interagency Volunteer Program: Helping Hands Across Public Lands – Phase Ii: Quarterly Progress Report, Period Covering January 1, 2012 To March 31, 2012, Margaret N. Rees

Get Outdoors Nevada

• The number of records in the volunteer database increased by 4.96% over the last quarter. The database now contains 7,423 records.
• Results show an average of 1,259 visits per month, and with an average of 3,015 pages viewed per month.
• Get Outdoors Nevada supported 14 volunteer events in a variety of ways. These events utilized 364 volunteers whom contributed approximately 2,414 hours of service.
• Three community outreach events were attended, resulting in 293 direct contacts with 50 names added to the database.
• The automated volunteer orientation training project has been completed and disks have been delivered to the 4 agencies. …

Reducing False-Positive Prediction Of Minimotifs With A Genetic Interaction Filter, Jerlin Camilus Merlin, Sanguthevar Rajasekaran, Tian Mi, Martin Schiller

Life Sciences Faculty Research

Background

Minimotifs are short contiguous peptide sequences in proteins that have known functions. At its simplest level, the minimotif sequence is present in a source protein and has an activity relationship with a target, most of which are proteins. While many scientists routinely investigate new minimotif functions in proteins, the major web-based discovery tools have a high rate of false-positive prediction. Any new approach that reduces false-positives will be of great help to biologists.

Methods and Findings

We have built three filters that use genetic interactions to reduce false-positive minimotif predictions. The basic filter identifies those minimotifs where the source/target …

2011 Research Highlights, Yusheng Zhao

NSTec UNLV Symposium

Overview

•Center Mission and Cluster Tasks
•Recent Scientific Achievements
•Current & Future Developments

School Program Planning To Increase Active Transport To School, Brenda Aguilar

McNair Poster Presentations

Studies have shown that active transport is known to increase physical activity of children, decrease traffic congestion and the production of greenhouse gasses. This study examines active transport to school (ATS) [walking, biking, or other self-powered wheels to school] among elementary students. Through the Nevada Moves Day program an increase proportion of elementary students who use ATS was expected.

This study was done at two elementary schools, one being the intervention school that participated in the Nevada Moves Day, and a control school, which did not participate. Data was collected over a three week period. Students using active transport and …

Survey Of Glycerol Dialkyl Glycerol Tetraethers (Gdgts) In Nevada And California Hot Springs And Selected T Thermophiles, Julienne J. Paraiso, Amanda Jean Williams, Brian P. Hedlund, Chuanlun L. Zhang

McNair Poster Presentations

Glycerol dialkyl glycerol tetraethers (GDGTs) are core membrane lipids of many Archaea and some Bacteria found ubiquitously in soils and in many aqueous environments. Here, we examined the GDGT concentration in forty sediment samples from geothermal hot springs in the Great Basin (USA). Sediment samples were collected in tandem with extensive geochemical and site characterization. Hot spring temperatures ranged from 31 to 95°C and pH values from 6.8 to 10.7. Parametric Pearson's correlation coefficients and nonparametric Spearman's rho values were calculated to identify significant correlations between GDGT profiles and geochemical analytes. Isoprenoidal GDGTs (iGDGTs) negatively correlated with pH and positively …

Transcriptome Analysis Of Glue Secretion In Drosophila, William Mccurdy, Pawel Parafianowicz, Andrew Andres

McNair Poster Presentations

Steroid hormones control important developmental and physiological responses in animals, including humans. It is known that when a cell is exposed to a steroid hormone, there is an immediate change in the genes that are expressed into proteins. Of notable importance is steroid regulation in the salivary glands of larval Drosophila melanogaster and the corresponding physiological responses that are governed by treatment with the conserved insect steroid, 20-hydroxyecdysone (20E). Exposure to the steroid hormone 20E causes a change in gene expression that facilitates the secretion of glue glycoproteins from inside the cells into the lumen of the tissue. Altered gene …

Minimotif Miner 3.0: Database Expansion And Significantly Improved Reduction Of False-Positive Predictions From Consensus Sequences., Tian Mi, Jerlin Camilus Merlin, Sandeep Deverasetty, Michael R. Gryk, Travis J. Bill, Andrew W. Brooks, Logan Lee, Viraj Rathnayake, Christian A. Ross, David P. Sargeant, Christy L. Strong, Paula Watts, Sanguthevar Rajasekaran, Martin Schiller

Life Sciences Faculty Research

Minimotif Miner (MnM available at http://minimotifminer.org or http://mnm.engr.uconn.edu) is an online database for identifying new minimotifs in protein queries. Minimotifs are short contiguous peptide sequences that have a known function in at least one protein. Here we report the third release of the MnM database which has now grown 60-fold to approximately 300,000 minimotifs. Since short minimotifs are by their nature not very complex we also summarize a new set of false-positive filters and linear regression scoring that vastly enhance minimotif prediction accuracy on a test data set. This online database can be used to predict new functions in proteins …

Dna Secondary Structures And Their Contribution To Mutagenesis In B. Subtilis Stationary Phase Cells, Carmen Vallin, Holly Anne Martin, Christian Ross, Ronald Yasbin, Eduardo Robleto

McNair Poster Presentations

It is widely known and accepted that the cause of many mutations in cells are generated during the replication process of actively dividing cells, however more recent research has shown that mutations also arise in non growing conditions, a phenomenon known as stationary phase mutagenesis. Much of what is known come from studies in eukaryotic and bacterial models. It has been proposed that in non-growing cells, the process of transcription plays an important role in mutagenesis. We test the hypothesis that DNA secondary structures, formed during transcription, promote mutagenesis. The transcription-generated structures are speculated to be prone to by blocking …