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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Pharmacologic Responses Of The Mouse Urinary Bladder, A. Erdem, Christopher Chapple, Russ Chess-Williams
Pharmacologic Responses Of The Mouse Urinary Bladder, A. Erdem, Christopher Chapple, Russ Chess-Williams
Russ Chess-Williams
The aim of the study was to determine pathways involved in contraction and relaxation of the mouse urinary bladder. Mouse bladder strips were set up in gassed Krebs-bicarbonate solution and responses to various drugs and electrical field stimulation were obtained. Isoprenaline (b-receptor agonist) caused a 63% inhibition of carbachol precontracted detrusor (EC50=2nM). Carbachol caused contraction (EC50=0.3µM), responses were antagonised more potently by 4-DAMP (M3-antagonist) than methoctramine (M2-antagonist). Electrical field stimulation caused contraction, which was inhibited by atropine (60%) and less by guanethidine and α,β-methylene-ATP. The neurogenic responses were not potentiated by inhibition of nitric oxide synthase. Presence of an intact …
Pharmacologic Responses Of The Mouse Urinary Bladder, A. Erdem, Christopher Chapple, Russ Chess-Williams
Pharmacologic Responses Of The Mouse Urinary Bladder, A. Erdem, Christopher Chapple, Russ Chess-Williams
Russ Chess-Williams
The aim of the study was to determine pathways involved in contraction and relaxation of the mouse urinary bladder. Mouse bladder strips were set up in gassed Krebs-bicarbonate solution and responses to various drugs and electrical field stimulation were obtained. Isoprenaline (b-receptor agonist) caused a 63% inhibition of carbachol precontracted detrusor (EC50=2nM). Carbachol caused contraction (EC50=0.3µM), responses were antagonised more potently by 4-DAMP (M3-antagonist) than methoctramine (M2-antagonist). Electrical field stimulation caused contraction, which was inhibited by atropine (60%) and less by guanethidine and α,β-methylene-ATP. The neurogenic responses were not potentiated by inhibition of nitric oxide synthase. Presence of an intact …
Effect Of Different Resistance Exercise Repetition Velocities On Excess Post-Exercise Oxygen Consumption And Energetic Expenditure, Ana Critine Barreto, Alex Souto Maior, Pedro Menzes, Jeffrey Willardson, Antonio Jose Silva, Victor Machado Reis, Roberto Simão, Jefferson Novaes
Effect Of Different Resistance Exercise Repetition Velocities On Excess Post-Exercise Oxygen Consumption And Energetic Expenditure, Ana Critine Barreto, Alex Souto Maior, Pedro Menzes, Jeffrey Willardson, Antonio Jose Silva, Victor Machado Reis, Roberto Simão, Jefferson Novaes
Jeffrey Willardson
The excess post-exercise oxygen consumption (EPOC) consists of the excess oxygen consumed above a resting state following exercise. Performance of resistance exercise can significantly disrupt the body’s homeostasis, with the EPOC being dependent on the specific combination of prescriptive variables. Presently, the effects of different repetition velocities on VO2 and caloric expenditure during and following resistance exercise bouts have not been completely elucidated. Objective: To examine the effect of different repetition velocities on EPOC and total energetic expenditure during and following resistance exercise bouts. Methods: Twenty women (34.6 ± 5.5 years; 159 ± 4.1 cm; 55.1 ± 3.4 kg; 24±2.5 …
Differences In The Mechanism Of Collagen Lattice Contraction By Myofibroblasts And Smooth Muscle Cells, J. C. Dallon, H P. Ehrlich
Differences In The Mechanism Of Collagen Lattice Contraction By Myofibroblasts And Smooth Muscle Cells, J. C. Dallon, H P. Ehrlich
Faculty Publications
Both rat derived vascular smooth muscle cells (SMC) and human myofibroblasts contain $\alpha$ smooth muscle actin (SMA), but they utilize different mechanisms to contract populated collagen lattices (PCLs). The difference is in how the cells generate the force that contracts the lattices. Human dermal fibroblasts transform into myofibroblasts, expressing $\alpha$-SMA within stress fibers, when cultured in lattices that remain attached to the surface of a tissue culture dish. When attached lattices are populated with rat derived vascular SMC, the cells retain their vascular SMC phenotype. Comparing the contraction of attached PCLs when they are released from the culture dish on …
Effect Of Different Resistance Exercise Repetition Velocities On Excess Post-Exercise Oxygen Consumption And Energetic Expenditure, Ana Critine Barreto, Alex Souto Maior, Pedro Menzes, Jeffrey Willardson, Antonio Jose Silva, Victor Machado Reis, Roberto Simão, Jefferson Novaes
Effect Of Different Resistance Exercise Repetition Velocities On Excess Post-Exercise Oxygen Consumption And Energetic Expenditure, Ana Critine Barreto, Alex Souto Maior, Pedro Menzes, Jeffrey Willardson, Antonio Jose Silva, Victor Machado Reis, Roberto Simão, Jefferson Novaes
Faculty Research and Creative Activity
The excess post-exercise oxygen consumption (EPOC) consists of the excess oxygen consumed above a resting state following exercise. Performance of resistance exercise can significantly disrupt the body’s homeostasis, with the EPOC being dependent on the specific combination of prescriptive variables. Presently, the effects of different repetition velocities on VO2 and caloric expenditure during and following resistance exercise bouts have not been completely elucidated. Objective: To examine the effect of different repetition velocities on EPOC and total energetic expenditure during and following resistance exercise bouts. Methods: Twenty women (34.6 ± 5.5 years; 159 ± 4.1 cm; 55.1 ± 3.4 kg; 24±2.5 …
Impaired M3 And Enhanced M2 Muscarinic Receptor Contractile Function In A Streptozotocin Model Of Mouse Diabetic Urinary Bladder, K. J. Pak, Rennolds S. Ostrom, M. Matsui, F. J. Ehlert
Impaired M3 And Enhanced M2 Muscarinic Receptor Contractile Function In A Streptozotocin Model Of Mouse Diabetic Urinary Bladder, K. J. Pak, Rennolds S. Ostrom, M. Matsui, F. J. Ehlert
Pharmacy Faculty Articles and Research
We investigated the contractile roles of M2 and M3 muscarinic receptors in urinary bladder from streptozotocin-treated mice. Wild-type and M2 muscarinic receptor knockout (M2 KO) mice were given a single injection of vehicle or streptozotocin (125 mg kg−1) 2–24 weeks prior to bladder assays. The effect of forskolin on contractions elicited to the muscarinic agonist, oxotremorine-M, was measured in isolated urinary bladder (intact or denuded of urothelium). Denuded urinary bladder from vehicle-treated wild-type and M2 KO mice exhibited similar contractile responses to oxotremorine-M, when contraction was normalized relative to that elicited by KCl (50 mM). Eight to 9 weeks after …