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Full-Text Articles in Life Sciences
Integrated Affinity Column Capillary Electrophoresis Microdevices For Biomarker Analysis, Weichun Yang
Integrated Affinity Column Capillary Electrophoresis Microdevices For Biomarker Analysis, Weichun Yang
Theses and Dissertations
In this dissertation, microfluidic systems that integrate antibody-based sample preparation methods with electrophoretic separation are developed to analyze multiple biomarkers in a point-of-care setting. To form an affinity column, both monolith materials and wall-coated channels were explored. I successfully demonstrated that monolith columns can be prepared in microfluidic devices via photopolymerization. The selectivity of monolith columns was improved by immobilizing antibodies on the surface. These affinity columns can selectively enrich target analytes and reduce the signal of contaminant proteins up to 25,000 fold after immunoaffinity extraction. These results clearly demonstrate that microchip affinity monoliths can selectively concentrate and purify target …
Validation Of Fingertip Whole Blood Against Common Blood Biomarkers Of Omega-3 Status In A Dose-Response Intervention, C E. Sparkes, A J. Sinclair, R A. Gibson, P L. Else, B J. Meyer
Validation Of Fingertip Whole Blood Against Common Blood Biomarkers Of Omega-3 Status In A Dose-Response Intervention, C E. Sparkes, A J. Sinclair, R A. Gibson, P L. Else, B J. Meyer
Faculty of Health and Behavioural Sciences - Papers (Archive)
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