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Diurnal Rhythms Of Behavior And Brain Mrna Expression For Arginine Vasotocin, Isotocin, And Their Receptors In Wild Amargosa Pupfish (Cyprinodon Nevadensis Amargosae), Sean C. Lema, Lauren J. Wagstaff, Nina M. Gardner
Diurnal Rhythms Of Behavior And Brain Mrna Expression For Arginine Vasotocin, Isotocin, And Their Receptors In Wild Amargosa Pupfish (Cyprinodon Nevadensis Amargosae), Sean C. Lema, Lauren J. Wagstaff, Nina M. Gardner
Biological Sciences
Amargosa pupfish (Cyprinodon nevadensis amargosae) occupy remote desert habitats that vary widely in environmental conditions from day to night. In this study, die! patterns of behavior were documented for pupfish in their natural habitat, and examined relative to changes in the abundance of mRNAs encoding prepro-arginine vasotocin (pro-VT), prepro-isotocin (pro-IT), three distinct vasotocin receptors (V1a1, V1a2, and V2), and an isotocin receptor (ITR) in the brain. The behavior of wild pupfish varied diurnally, with frequent aggression from 12:00 to 15:00 h and courtship and spawning most common between 15:00 and 19:00h. Transcript abundance for pro-VT in the brain …
Identification Of Multiple Vasotocin Receptor Cdnas In Teleost Fish: Sequences, Phylogenetic Analysis, Sites Of Expression, And Regulation In The Hypothalamus And Gill In Response To Hyperosmotic Challenge, Sean C. Lema
Biological Sciences
Vasopressin and its homolog vasotocin regulate hydromineral balance, stress responses, and social behaviors in vertebrates. In mammals, the functions of vasopressin are mediated via three classes of membrane-bound receptors: V1a-type, V1b-type and V2-type. To date, however, only a single class of vasotocin receptor has been identified in teleost fish. Here, cDNAs encoding three putative vasotocin receptors – two distinct V1a-type receptor paralogs (V1a1 and V1a2) and a previously undescribed V2- type receptor (V2) – and a single isotocin receptor were isolated and sequenced from the Amargosa pupfish (Cyprinodon nevadensis amargosae). RT-PCR revealed that mRNAs …