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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Attempted Cloning Of A Wnt Gene From Botrylloides Violaceus, Manasa Chandra, James Tumulak
Attempted Cloning Of A Wnt Gene From Botrylloides Violaceus, Manasa Chandra, James Tumulak
Biological Sciences
Botrylloides violaceus is a colonial ascidian with the ability to undergo sexual and asexual reproduction as well as regeneration. The canonical pathway starts with the extracellular protein Wnt and ends with β-catenin, a transcription factor, which also functions in cell adhesion. The Wnt signaling pathway is involved in embryogenesis and regeneration in a variety of other species. In our studies we attempt to isolate and sequence both a Wnt gene and from Botrylloides via degenerate primer design and PCR. Using bioinformatic methods we aligned sequences from other organisms, as the Botrylloides genome has not yet been sequenced. Using mouse, Ciona, …
Lyophilization Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Larvae Yields High-Quality Dna For Use In Aflp Genetic Studies, Pete L. Clark, David J. Isenhour, Steven R. Skoda, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster
Lyophilization Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Larvae Yields High-Quality Dna For Use In Aflp Genetic Studies, Pete L. Clark, David J. Isenhour, Steven R. Skoda, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster
Department of Entomology: Faculty Publications
Agricultural research in the 21st century has become a collaborative effort. Research on crop pests like Spodoptera frugiperda (J.E. Smith), commonly known as the fall armyworm (FAW), can involve international collaboration because it is a pest not only in the southern United States, but also in Latin and South America. Our interest to study the genetic variation of 24 subpopulations of FAW from the southern United States, Mexico, Puerto Rico, Brazil and Argentina required insect collection procedures that preserve the integrity of DNA for molecular genetic analysis. The samples were collected primarily from maize (Zea mays L.), but also …
Detection Of Bordetella Avium By Polymerase Chain Reaction In The Lungs And Tracheas Of Turkeys With Pneumonia, Süheyla Türkyilmaz, Sevi̇n Kirdar, Fulya Ocak, Şebnur Hazimoğlu
Detection Of Bordetella Avium By Polymerase Chain Reaction In The Lungs And Tracheas Of Turkeys With Pneumonia, Süheyla Türkyilmaz, Sevi̇n Kirdar, Fulya Ocak, Şebnur Hazimoğlu
Turkish Journal of Veterinary & Animal Sciences
Lung, trachea, and serum samples obtained from turkeys showing clinical signs of respiratory disease were examined for the presence of Bordetella avium infection (bordetellosis) using culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). The material of this study consisted of 2 live turkeys at the age of 24 weeks with respiratory distress, nasal discharge, and cough that had been brought to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Adnan Menderes University. The results of the study show that B. avium, the causative agent of bordetellosis, was not isolated from either the lung or trachea, while both …
Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n
Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to identify the presence and prevalence of bovine tuberculosis in the eastern Anatolian border province of Van and its villages and to collect the preliminary data required for programs for the prevention and control of this zoonotic disease. The tuberculin test was performed on 210 cattle. Nasal swab and milk samples were obtained from the animals tested. Three cattle that had a positive tuberculin test also tested positive for the DNA target, a 580-bp fragment of IS6110 specific for members of the Mycobacterium tuberculosis complex. This fragment was also recovered from milk taken from …
Molecular Characterization And Genetic Diversity Analysis Of Citrus Cultivars By Rapd Markers, M. N. R. Baig, Sapna Grewal, Santosh Dhillon
Molecular Characterization And Genetic Diversity Analysis Of Citrus Cultivars By Rapd Markers, M. N. R. Baig, Sapna Grewal, Santosh Dhillon
Turkish Journal of Agriculture and Forestry
Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers; of which 231 were polymorphic and some species or cultivar specific RAPD markers. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated Jatti-Khatti from all major clusters at a similarity coefficient of 0.61. The average genetic similarity value observed across all the genotypes was 0.63, with the 2 sweet orange cultivars, Jaffa …
Putative Agents Of Fig Mosaic Disease In Turkey, Kadri̇ye Çağlayan, Vicente Medina, Mona Gazel, Çi̇ğdem U. Serçe, Luis Serrano, Angeles Achon, Soner Soylu, Oğuzhan Çalişkan, Mustafa Gümüş
Putative Agents Of Fig Mosaic Disease In Turkey, Kadri̇ye Çağlayan, Vicente Medina, Mona Gazel, Çi̇ğdem U. Serçe, Luis Serrano, Angeles Achon, Soner Soylu, Oğuzhan Çalişkan, Mustafa Gümüş
Turkish Journal of Agriculture and Forestry
Leaves from 58 fig tree accessions showing different severity of mosaic and necrotic symptoms were collected in 2 different regions of Turkey and analysed by transmission electron microscope (TEM) and RT-PCR to determine putative causal agents of fig mosaic disease (FMD). Ultrastructural studies showed that double membrane-bound bodies (DMBs) were mainly observed in the parenchyma and mesophyll cells of infected leaves of the cultivars Göklop and Morgüz and some seedlings showing only mosaic symptoms. These bodies were surrounded by a fibrillar matrix in most of the infected cells. Long, flexuous rod-shaped virus-like particles (LFPs) were also observed in vascular tissues …