Life Sciences Commons^™

Functional Transition In The Floral Receptacle Of The Sacred Lotus (Nelumbo Nucifera): From Thermogenesis To Photosynthesis, R. E. Miller, J. R. Watling, Sharon A. Robinson

Sharon Robinson

The receptacle of the sacred lotus is the main source of heat during the thermogenic stage of floral development. Following anthesis, it enlarges, greens and becomes a fully functional photosynthetic organ. We investigated development of photosynthetic traits during this unusual functional transition. There were two distinct phases of pigment accumulation in receptacles. Lutein and photoprotective xanthophyll cycle pigments accumulated first with 64% and 95% of the maximum, respectively, present prior to anthesis. Lutein epoxide comprised 32% of total carotenoids in yellow receptacles, but declined with development. By contrast, more than 85% of maximum total chlorophyll, β-carotene and Rubisco were produced …

Responses Of Plants In Polar Regions To Uvb Exposure : A Meta-Analysis, K. K. Newsham, Sharon A. Robinson

Sharon Robinson

We report a meta-analysis of data from 34 field studies into the effects of UV-B radiation on Arctic and Antarctic bryophytes and angiosperms. The studies measured plant responses to decreases in UV-B radiation under screens, natural fluctuations in UV-B irradiance, or increases in UV-B radiation applied from fluorescent UV lamps. Exposure to UV-B radiation was found to increase the concentrations of UV-B absorbing compounds in leaves or thalli by 7% and 25% (expressed on a mass or area basis, respectively). UV-B exposure also reduced aboveground biomass and plant height by 15% and 10%, respectively, and increased DNA damage by 90%. …

Genetic Structure Of East Antarctic Populations Of The Moss Ceratodon Purpureus, L. J. Clarke, D. J. Ayre, Sharon A. Robinson

Sharon Robinson

The capacity of the polar flora to adapt is of increasing concern given current and predicted environmental change in these regions. Previous genetic studies of Antarctic mosses have been of limited value due to a lack of variation in the markers or non-specificity of the methods used. We examined the power of five microsatellite loci developed for the cosmopolitan moss Ceratodon purpureus to detect genetically distinct clones and infer the distribution of clones within and among populations from the Windmill Islands, East Antarctica. Our microsatellite data suggest extraordinarily high levels of variation reported in RAPD studies were artificially elevated by …

Two Cys Or Not Two Cys? That Is The Question; Alternative Oxidase In The Thermogenic Plant Sacred Lotus, Nicole M. Grant, Yoshihiko Onda, Yusuke Kakizaki, Kkikukatsu Ito, Jennifer R. Watling, Sharon A. Robinson

Sharon Robinson

Sacred lotus (Nelumbo nucifera) regulates temperature in its floral chamber to 32°C to 35°C across ambient temperatures of 8°C to 40°C with heating achieved through high alternative pathway fluxes. In most alternative oxidase (AOX) isoforms, two cysteine residues, Cys1 and Cys2, are highly conserved and play a role in posttranslational regulation of AOX. Further control occurs via interaction of reduced Cys1 with α-keto acids, such as pyruvate. Here, we report on the in vitro regulation of AOX isolated from thermogenic receptacle tissues of sacred lotus. AOX protein was mostly present in the reduced form, and only a small fraction could …

Surface Reflectance Properties Of Antarctic Moss And Their Relationship To Plant Species, Pigment Composition And Photosynthetic Function, C. E. Lovelock, Sharon A. Robinson

Sharon Robinson

We investigated how surface reflectance properties and pigment concentrations of Antarctic moss varied over species, sites, microtopography, and with water content. We found that species had significantly different surface reflectance properties, particularly in the region of the red edge (approximately 700 nm), but this did not correlate strongly with pigment concentrations. Surface reflectance of moss also varied in the visible region and in the characteristics of the red edge over different sites. Reflectance parameters, such as the Photochemical Reflectance Index (PRI) and Cold Hard Band (CBH) were useful discriminators of site, microtopographic position and water content. The PRI was correlated …

Understanding The Tolerance Of Antarctic Mosses To Climate Change, Sharon A. Robinson, L J. Clarke

Sharon Robinson

Climate change is expected to affect the high latitudes first and most severely, rendering Antarctica one of the most significant baseline environments for the study of global climate change. Despite this, there have been few long-term studies of the response of Antarctic vegetation to climate change. The Windmill Islands region supports some of the most extensive and best developed vegetation on continental Antarctica, with lush, green mossbeds along many of the lakes and melt streams close to Casey station. Over the past 12 years my University of Wollongong colleagues and I have studied the mosses of this region to better …

Synchronicity Of Thermogenic Activity, Alternative Pathway Respiratory Flux, Aox Protein Content, And Carbohydrates In Receptacle Tissues Of Sacred Lotus During Floral Development, Nicole M. Grant, Rebecca E. Miller, Jennifer R. Watling, Sharon A. Robinson

Sharon Robinson

The relationships between heat production, alternative oxidase (AOX) pathway flux, AOX protein, and carbohydrates during floral development in Nelumbo nucifera (Gaertn.) were investigated. Three distinct physiological phases were identified: pre-thermogenic, thermogenic, and post-thermogenic. The shift to thermogenic activity was associated with a rapid, 10-fold increase in AOX protein. Similarly, a rapid decrease in AOX protein occurred post-thermogenesis. This synchronicity between AOX protein and thermogenic activity contrasts with other thermogenic plants where AOX protein increases some days prior to heating. AOX protein in thermogenic receptacles was significantly higher than in post-thermogenic and leaf tissues. Stable oxygen isotope measurements confirmed that the …

Uv-B Screening Potential Is Higher In Two Cosmopolitan Moss Species Than In A Co-Occurring Antarctic Endemic Moss – Implications Of Continuing Ozone Depletion, J. L. Dunn, Sharon A. Robinson

Sharon Robinson

Concentrations of UV-B absorbing pigments and anthocyanins were measured in three moss species, over a summer growing season in Antarctica. Pigment concentrations were compared with a range of climatic variables to determine if there was evidence that pigments were induced by UV-B radiation, or other environmental parameters, and secondly if there were differences between species in their pigment responses. Significant seasonal differences in the potential UV-B screening pigments were found, with the two cosmopolitan species Bryum pseudotriquetrum and Ceratodon purpureus appearing better protected from the potentially damaging effects of ozone depletion than the Antarctic endemic Schistidium antarctici. Bryum pseudotriquetrum accumulated …

Contribution Of The Alternative Pathway To Respiration During Thermogenesis In Flowers Of The Sacred Lotus, Jennifer R. Watling, Sharon A. Robinson, Roger S. Seymour

Sharon Robinson

We report results from in vivo measurements, using oxygen isotope discrimination techniques, of fluxes through the alternative and cytochrome respiratory pathways in thermogenic plant tissue, the floral receptacle of the sacred lotus (Nelumbo nucifera). Fluxes through both pathways were measured in thermoregulating flowers undergoing varying degrees of thermogenesis in response to ambient temperature. Significant increases in alternative pathway flux were found in lotus receptacles with temperatures 16°C to 20°C above ambient, but not in those with lesser amounts of heating. Alternative pathway flux in the hottest receptacles was 75% of the total respiratory flux. In contrast, fluxes through the cytochrome …

Diversity - Images Of Nature (Exhibition), S. A. Robinson, L. Tasker, J. Wright, A. Netherwood

Sharon Robinson

Exhibition of photographs by Sharon Robinson, Liz Tasker, Andrew Netherwood and Julie Wright, held at the University of Wollongong Library, February 2009. Web gallery of exhibition images available here:

http://www.ndesign.net.au/DiversityExhibition/index.html

While the exhibition is underpinned by scientific research the images also reflect the various photographers fascination with how animals and plants manage to survive and flourish in an incredible range of environments around the world.