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Articles 1 - 30 of 604
Full-Text Articles in Life Sciences
Splenic Stromal Niches Support Hematopoiesis Of Dendritic-Like Cells From Precursors In Bone Marrow And Spleen., Pravin Periasamy, Jonathan Tan, Kristin Griffiths, Helen O'Neill
Splenic Stromal Niches Support Hematopoiesis Of Dendritic-Like Cells From Precursors In Bone Marrow And Spleen., Pravin Periasamy, Jonathan Tan, Kristin Griffiths, Helen O'Neill
Jonathan Tan
Objective The aims of this study are to test the ability of stromal cells from murine spleen to support hematopoiesis, to define the tissue source of precursors that seed these hematopoietic niches, and to determine the type of cells produced. Materials and Methods Cloned isolates of murine spleen stroma have been developed that support hematopoiesis. Analysis has been investigated in terms of tissue source of progenitors. Type and number of cells produced were analyzed by flow cytometry. Results Hematopoietic precursors that seed cocultures exist in spleen and bone marrow (BM), but not thymus. Cell production is highest if overlay cells …
A Laser Pointer Driven Microheater For Precise Local Heating And Conditional Gene Regulation In Vivo. Microheater Driven Gene Regulation In Zebrafish, Mike Placinta, Meng-Chieh Shen, Marc Achermann, Rolf O. Karlstrom
A Laser Pointer Driven Microheater For Precise Local Heating And Conditional Gene Regulation In Vivo. Microheater Driven Gene Regulation In Zebrafish, Mike Placinta, Meng-Chieh Shen, Marc Achermann, Rolf O. Karlstrom
Rolf O Karlstrom
Background - Tissue heating has been employed to study a variety of biological processes, including the study of genes that control embryonic development. Conditional regulation of gene expression is a particularly powerful approach for understanding gene function. One popular method for mis-expressing a gene of interest employs heat-inducible heat shock protein (hsp) promoters. Global heat shock of hsp-promoter-containing transgenic animals induces gene expression throughout all tissues, but does not allow for spatial control. Local heating allows for spatial control of hsp-promoter-driven transgenes, but methods for local heating are cumbersome and variably effective. Results - We describe a simple, highly controllable, …
Dust Emissions From Undisturbed And Disturbed, Crusted Playa Surfaces: Cattle Trampling Effect, Ed Frederickson, Teddy Zobeck, Matthew Baddock, Robert Van Pelt
Dust Emissions From Undisturbed And Disturbed, Crusted Playa Surfaces: Cattle Trampling Effect, Ed Frederickson, Teddy Zobeck, Matthew Baddock, Robert Van Pelt
Ed L. Frederickson
Dry playa lake beds can be a significant source of fine dust emissions during high wind events in arid and semiarid landscapes. The physical and chemical properties of the playa surface control the amount and properties of the dust emitted. In this study, we use a field wind tunnel to quantify the dust emissions from a bare, fine-textured playa surface located in the Chihuahua Desert at the Jornada Experimental Range, near Las Cruces, New Mexico, USA. We tested natural, undisturbed crusted surfaces and surfaces that had been subjected to two levels of domestic animal disturbance. The animal disturbance was provided …
Rudra Interrupts Receptor Signaling Complexes To Negatively Regulate The Imd Pathway, Kamna Aggarwal, Florentina Rus, Christie Vriesema-Magnuson, Deniz Erturk Hasdemir, Nicholas Paquette, Neal S. Silverman
Rudra Interrupts Receptor Signaling Complexes To Negatively Regulate The Imd Pathway, Kamna Aggarwal, Florentina Rus, Christie Vriesema-Magnuson, Deniz Erturk Hasdemir, Nicholas Paquette, Neal S. Silverman
Neal Silverman
Insects rely primarily on innate immune responses to fight pathogens. In Drosophila, antimicrobial peptides are key contributors to host defense. Antimicrobial peptide gene expression is regulated by the IMD and Toll pathways. Bacterial peptidoglycans trigger these pathways, through recognition by peptidoglycan recognition proteins (PGRPs). DAP-type peptidoglycan triggers the IMD pathway via PGRP-LC and PGRP-LE, while lysine-type peptidoglycan is an agonist for the Toll pathway through PGRP-SA and PGRP-SD. Recent work has shown that the intensity and duration of the immune responses initiating with these receptors is tightly regulated at multiple levels, by a series of negative regulators. Through two-hybrid screening …
Fighting Infection Fly-Style, Louisa Wu, Neal S. Silverman
Fighting Infection Fly-Style, Louisa Wu, Neal S. Silverman
Neal Silverman
No abstract provided.
Nf-Kappab Signaling Pathways In Mammalian And Insect Innate Immunity, Neal S. Silverman, Tom Maniatis
Nf-Kappab Signaling Pathways In Mammalian And Insect Innate Immunity, Neal S. Silverman, Tom Maniatis
Neal Silverman
In this review, we discuss recent advances in understanding the signaling pathways in mammalian and Drosophila innate immunity, with emphasis on the mechanisms by which NF-kappaB/Rel family proteins are activated.
Hormonal Regulation Of The Humoral Innate Immune Response In Drosophila Melanogaster, Thomas Flatt, Andreas Heyland, Florentina Rus, Ermelinda Porpiglia, Chris Sherlock, Rochele Yamamoto, Alina Garbuzov, Subba R. Palli, Marc Tatar, Neal S. Silverman
Hormonal Regulation Of The Humoral Innate Immune Response In Drosophila Melanogaster, Thomas Flatt, Andreas Heyland, Florentina Rus, Ermelinda Porpiglia, Chris Sherlock, Rochele Yamamoto, Alina Garbuzov, Subba R. Palli, Marc Tatar, Neal S. Silverman
Neal Silverman
Juvenile hormone (JH) and 20-hydroxy-ecdysone (20E) are highly versatile hormones, coordinating development, growth, reproduction and aging in insects. Pulses of 20E provide key signals for initiating developmental and physiological transitions, while JH promotes or inhibits these signals in a stage-specific manner. Previous evidence suggests that JH and 20E might modulate innate immunity, but whether and how these hormones interact to regulate the immune response remains unclear. Here we show that JH and 20E have antagonistic effects on the induction of antimicrobial peptide (AMP) genes in Drosophila melanogaster. 20E pretreatment of Schneider S2 cells promoted the robust induction of AMP genes, …
Consent To The Use Of Stored Dna For Genetics Research: A Survey Of Attitudes In The Jewish Population, Marc D. Schwartz, Karen H. Rothenberg, Linda Joseph, Judith Benkendorf, Caryn Lerman
Consent To The Use Of Stored Dna For Genetics Research: A Survey Of Attitudes In The Jewish Population, Marc D. Schwartz, Karen H. Rothenberg, Linda Joseph, Judith Benkendorf, Caryn Lerman
Karen H. Rothenberg
No abstract provided.
Judging Genes: Implications Of The Second Generation Of Genetic Tests In The Courtroom, Diane E. Hoffmann, Karen H. Rothenberg
Judging Genes: Implications Of The Second Generation Of Genetic Tests In The Courtroom, Diane E. Hoffmann, Karen H. Rothenberg
Karen H. Rothenberg
The use of DNA tests for identification has revolutionized court proceedings in criminal and paternity cases. Now, requests by litigants to admit or compel a second generation of genetic tests – tests to confirm or predict genetic diseases and conditions – threaten to affect judicial decision-making in many more contexts. Unlike DNA tests for identification, these second generation tests may provide highly personal health and behavioral information about individuals and their relatives and will pose new challenges for trial court judges. This article reports on an original empirical study of how judges analyze these requests and uses the study results …
Social Implications Of Genetic Testing, Karen H. Rothenberg
Social Implications Of Genetic Testing, Karen H. Rothenberg
Karen H. Rothenberg
This paper examines the social implications of predictive genetic testing and its impact on the insurance industry. Although the Human Genome Project has the potential to improve the health of our nation, it also may serve as a means of highlighting genetic differences among individuals and ethnic groups. Thus, if we are to reach the full promise of the Project, society must address the public's fears of genetic discrimination in insurance and employment context. Following an analysis of state and federal legislation on genetic privacy and discrimination, the paper concludes with a challenge to the insurance industry to work with …
Genetic Information And The Workplace: Legislative Approaches And Policy Challenges, Karen H. Rothenberg, Barbara Fuller, Mark Rothstein, Troy Duster, Mary Jo Ellis Kahn, Rita Cunningham, Beth Fine, Kathy Hudson, Mary-Claire King, Patricia Murphy, Gary Swergold, Francis Collins
Genetic Information And The Workplace: Legislative Approaches And Policy Challenges, Karen H. Rothenberg, Barbara Fuller, Mark Rothstein, Troy Duster, Mary Jo Ellis Kahn, Rita Cunningham, Beth Fine, Kathy Hudson, Mary-Claire King, Patricia Murphy, Gary Swergold, Francis Collins
Karen H. Rothenberg
No abstract provided.
The Scarlet Gene: Behavioral Genetics, Criminal Law, And Racial And Ethnic Stigma, Karen H. Rothenberg, Alice Wang
The Scarlet Gene: Behavioral Genetics, Criminal Law, And Racial And Ethnic Stigma, Karen H. Rothenberg, Alice Wang
Karen H. Rothenberg
Imagine that a scientist from the state university asks you and your family to participate in a study on a particular gene variant associated with alcoholism. The project focuses on your ethnic group, the Tracy Islanders, who have a higher incidence of alcoholism, as well as a higher incidence of the gene variant, than the general population. You will not be informed whether you have the gene variant, but your participation in the study might help scientists develop drugs to help individuals control their addiction to alcohol. You have a family history of alcoholism, and you are concerned that your …
When Should Judges Admit Or Compel Genetic Tests?, Diane E. Hoffmann, Karen H. Rothenberg
When Should Judges Admit Or Compel Genetic Tests?, Diane E. Hoffmann, Karen H. Rothenberg
Karen H. Rothenberg
No abstract provided.
Cancer Genetic Susceptibility Testing: Ethical And Policy Implications For Future Research And Clinical Practice, Benjamin S. Wilfond, Karen H. Rothenberg, Elizabeth J. Thomson, Caryn Lerman
Cancer Genetic Susceptibility Testing: Ethical And Policy Implications For Future Research And Clinical Practice, Benjamin S. Wilfond, Karen H. Rothenberg, Elizabeth J. Thomson, Caryn Lerman
Karen H. Rothenberg
No abstract provided.
Toward A Framework Of Mutualism: The Jewish Community In Genetics Research, Karen H. Rothenberg, Amy B. Rutkin
Toward A Framework Of Mutualism: The Jewish Community In Genetics Research, Karen H. Rothenberg, Amy B. Rutkin
Karen H. Rothenberg
No abstract provided.
Manifest Greatness Version5 By Marc Guerrero With Tato Malay, Emmanuel Mario B. Santos Aka Marc Guerrero
Manifest Greatness Version5 By Marc Guerrero With Tato Malay, Emmanuel Mario B. Santos Aka Marc Guerrero
Emmanuel Mario B Santos aka Marc Guerrero
MANIFEST GREATNESS version5 by Marc Guerrero with Tato Malay
Manifest Greatness Version3 By Marc Guerrero With Jay Fajardo, Emmanuel Mario B. Santos Aka Marc Guerrero
Manifest Greatness Version3 By Marc Guerrero With Jay Fajardo, Emmanuel Mario B. Santos Aka Marc Guerrero
Emmanuel Mario B Santos aka Marc Guerrero
MANIFEST GREATNESS version3 by Marc Guerrero with Jay Fajardo
Manifest Greatness Version2 With Danielle Van Asch-Prevot, Emmanuel Mario B. Santos Aka Marc Guerrero
Manifest Greatness Version2 With Danielle Van Asch-Prevot, Emmanuel Mario B. Santos Aka Marc Guerrero
Emmanuel Mario B Santos aka Marc Guerrero
MANIFEST GREATNESS version2 by Marc Guerrero with Danielle van Asch-Prevot
Nucleotide And Phospholipid-Dependent Control Of Ppxd And C-Domain Association For Seca Atpase, Don Oliver
Nucleotide And Phospholipid-Dependent Control Of Ppxd And C-Domain Association For Seca Atpase, Don Oliver
Don Oliver
The SecA ATPase motor is a central component of the eubacterial protein translocation machinery. It is comprised of N- and C-domain substructures, where the N-domain is comprised of two nucleotide-binding domains that flank a preprotein-binding domain (PPXD), while the C-domain binds phospholipids as well as SecB chaperone. Our recent crystal structure of Bacillus subtilis SecA protomer [Hunt, J. F., Weinkauf, S., Henry, L., Fak, J. J., McNicholas, P., Oliver, D. B., and Deisenhofer, J. (2002) Science 297, 2018-2026] along with experimental support for the correct dimer structure [Ding, H., Hunt, J. F., Mukerji, I., and Oliver, D. (2003) Biochemistry 42, …
Role Of A Conserved Glutamate Residue In The Escherichia Coli Seca Atpase Mechanism, Don Oliver
Role Of A Conserved Glutamate Residue In The Escherichia Coli Seca Atpase Mechanism, Don Oliver
Don Oliver
Escherichia coli SecA uses ATP to drive the transport of proteins across cell membranes. Glutamate 210 in the "DEVD" Walker B motif of the SecA ATP-binding site has been proposed as the catalytic base for ATP hydrolysis (Hunt, J. F., Weinkauf, S., Henry, L., Fak, J. J., McNicholas, P., Oliver, D. B., and Deisenhofer, J. (2002) Science 297, 2018-2026). Consistent with this hypothesis, we find that mutation of glutamate 210 to aspartate results in a 90-fold reduction of the ATP hydrolysis rate compared with wild type SecA, 0.3 s(-1) versus 27 s(-1), respectively. SecA-E210D also releases ADP at a slower …
Dimeric Seca Is Essential For Protein Translocation, Don Oliver
Dimeric Seca Is Essential For Protein Translocation, Don Oliver
Don Oliver
SecA facilitates bacterial protein translocation by its association with presecretory or membrane proteins and the SecYEG translocon channel. Once assembled, SecA ATPase undergoes cycles of membrane insertion and retraction at SecYEG that drive protein translocation in a stepwise fashion. SecA exists in equilibrium between a monomer and dimer, and association with its translocation ligands shifts this equilibrium dramatically. Here, we examined the proposal that protein translocation can occur by means of a SecA monomer. We produced a mutant SecA protein lacking residues 2-11, which was found to exist mostly as a monomer, and it was unable to complement a conditional-lethal …
Varriation Of Growth Rates In Yellow-Bellied Marmots, Carmen M. Salsbury, K. B. Armitage
Varriation Of Growth Rates In Yellow-Bellied Marmots, Carmen M. Salsbury, K. B. Armitage
Carmen M. Salsbury
Growth rates of yellow-bellied marmot (Marmota flaviventris) populations over a 32-year period (1965 -1996) varied Significantly with sex, age, location, and year. Overall, males had higher growth rates than females and young and yearlings generally had higher growth rates compared to adults at all locations. The locations varied with respect to elevation and the relationship between elevation and growth rate was complex and likely weather dependent. Low rainfall in late summer was often associated with low growth rates at high elevations where the active season is constrained and delayed by late spring snowmelt compared to low elevations. Growth rates and …
Varriation Of Growth Rates In Yellow-Bellied Marmots, Carmen M. Salsbury, K. B. Armitage
Varriation Of Growth Rates In Yellow-Bellied Marmots, Carmen M. Salsbury, K. B. Armitage
Carmen M. Salsbury
Growth rates of yellow-bellied marmot (Marmota flaviventris) populations over a 32-year period (1965 -1996) varied Significantly with sex, age, location, and year. Overall, males had higher growth rates than females and young and yearlings generally had higher growth rates compared to adults at all locations. The locations varied with respect to elevation and the relationship between elevation and growth rate was complex and likely weather dependent. Low rainfall in late summer was often associated with low growth rates at high elevations where the active season is constrained and delayed by late spring snowmelt compared to low elevations. Growth rates and …
Manifest Greatness... Panahon Ng Mga Filipino Ang 21st Century: Ang Asian Century (Ang Pagpapanumbalik Sa Likas Na Karangalan Ng Lahat Ng Filipino Sa Buong Mundo), Emmanuel Mario B. Santos Aka Marc Guerrero
Manifest Greatness... Panahon Ng Mga Filipino Ang 21st Century: Ang Asian Century (Ang Pagpapanumbalik Sa Likas Na Karangalan Ng Lahat Ng Filipino Sa Buong Mundo), Emmanuel Mario B. Santos Aka Marc Guerrero
Emmanuel Mario B Santos aka Marc Guerrero
MANIFEST GREATNESS Panahon ng mga Filipino ang 21st century: Ang Asian Century (Ang pagpapanumbalik sa likas na Karangalan ng lahat ng Filipino sa buong mundo) Manifest Greatness is a work-in-progress Manifesto of, for and by Filipino citizens of the world in synergy with foreign national friends of the Filipino people worldwide in pursuit of genuine entrepreneurial wisdom
Muc4/Muc4 Functions And Regulation In Cancer., Goldi Kozloski
Muc4/Muc4 Functions And Regulation In Cancer., Goldi Kozloski
Goldi A Kozloski
Emerging Role Of Micrornas In Liver Diseases, Shashi Bala, Miguel Marcos, Gyongyi Szabo
Emerging Role Of Micrornas In Liver Diseases, Shashi Bala, Miguel Marcos, Gyongyi Szabo
Gyongyi Szabo
MicroRNAs are a class of small non-coding RNAs that are found in plants, animals, and some viruses. They modulate the gene function at the post-transcriptional level and act as a fine tuner of various processes, such as development, proliferation, cell signaling, and apoptosis. They are associated with different types and stages of cancer. Recent studies have shown the involvement of microRNAs in liver diseases caused by various factors, such as Hepatitis C, Hepatitis B, metabolic disorders, and by drug abuse. This review highlights the role of microRNAs in liver diseases and their potential use as therapeutic molecules.
Reexamination Of The Role Of The Amino Terminus Of Seca In Promoting Its Dimerization And Functional State, Don Oliver
Reexamination Of The Role Of The Amino Terminus Of Seca In Promoting Its Dimerization And Functional State, Don Oliver
Don Oliver
No abstract provided.
Seca Dimer Cross-Linked At Its Subunit Interface Is Functional For Protein Translocation, Don Oliver
Seca Dimer Cross-Linked At Its Subunit Interface Is Functional For Protein Translocation, Don Oliver
Don Oliver
SecA facilitates protein transport across the eubacterial plasma membrane by its association with cargo proteins and the SecYEG translocon, followed by ATP-driven conformational changes that promote protein translocation in a stepwise manner. Whether SecA functions as a monomer or a dimer during this process has been the subject of considerable controversy. Here we utilize cysteine-directed mutagenesis along with the crystal structure of the SecA dimer to create a cross-linked dimer at its subunit interface, which was normally active for in vitro protein translocation.
In Vivo Membrane Topology Of Escherichia Coli Seca Atpase Reveals Extensive Periplasmic Exposure Of Multiple Functionally Important Domains Clustering On One Face Of Seca, Don Oliver
Don Oliver
No abstract provided.
Effect Of Superconsciousness External Energy On The Atomic, Crystalline And Powder Characterisitics Of “Carbon Allotrope Powders”, Mahendra Kumar Trivedi
Effect Of Superconsciousness External Energy On The Atomic, Crystalline And Powder Characterisitics Of “Carbon Allotrope Powders”, Mahendra Kumar Trivedi
Mahendra Kumar Trivedi
Scientists are searching for eluding link between spirituality and science. Some believe fundamental essences of universe to be energy and information. As per current understanding energy and matter always co- existed and is considered one and the same. Energy is consideredas ‘matter-inperpetual-motion’and matter as ‘stationary-energy’.Inter conversion between matter and energy has been defined by Einstein’s famous energy-mass equation (E=mc2) which has been provedby nuclear physicists using complex nuclear reactions involving high energy particles. However, many spiritual masters have claimed to realize this energy-matter inter conversion using their spiritual powers/energy but scientifically unknown and unverified. It is the first time that …