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Full-Text Articles in Life Sciences
The Regulation Of The Icsp Promoter Of Shigella Flexneri By The Virulence Factor Virb, Maria Castellanos, Dustin Harrison, Helen Wing
The Regulation Of The Icsp Promoter Of Shigella Flexneri By The Virulence Factor Virb, Maria Castellanos, Dustin Harrison, Helen Wing
Undergraduate Research Opportunities Program (UROP)
Shigella flexneri is a pathogenic bacterium that causes severe dysentery in humans commonly known as shigellosis. Shigella encodes an outer membrane protease called IcsP. The regulation of icsP expression is under direct control of a transcriptional factor called VirB, which controls the expression of many virulence genes in Shigella. Previous work has shown through deletion analysis of the icsP promoter region that sequences as far as 1368 base pairs upstream of the transcription starting site are important for the regulation of the icsP gene by VirB. However, it is still unclear whether VirB activation requires sequences within the icsP promoter …
The Effects Of Host Physiological Conditions On The Expression Of Icsp In Shigella Flexneri, Karen Levy, Helen Wing
The Effects Of Host Physiological Conditions On The Expression Of Icsp In Shigella Flexneri, Karen Levy, Helen Wing
Undergraduate Research Opportunities Program (UROP)
Shigella flexneri is a gram-negative bacterium capable of causing diarrhea and dysentery known as shigellosis. It is estimated there are 167.4 million shigellosis episodes throughout the world each year causing 1.1 million deaths. Shigella invades cells in the lower intestine through an induced phagocytosis. Once in the cytoplasm, bacteria move from one cell to another using actin-based motility. The Shigella outer membrane protease IcsP regulates actin-based motility and cell-to-cell spread by cleaving the actin assembly protein IcsA from the bacterial cell surface. We hypothesize that IcsP may serve additional functions during infection. By examining which environmental signals trigger icsP expression, …
Exploring The Role Of Iscsua And Suf Operon In Shigella Flexneri Invasion And Resistance To Oxidative Stress, Benjamin R. Lloyd
Exploring The Role Of Iscsua And Suf Operon In Shigella Flexneri Invasion And Resistance To Oxidative Stress, Benjamin R. Lloyd
Honors Theses
Shigella flexneri is a facultative intracellular pathogen capable of causing dysentery, a condition that afflicts many around the world, especially in developing countries. There are several aspects of Shigella pathogenesis that are not well understood, including a number of genetic and cellular changes that allow Shigella to adapt to stresses encountered while invading and replicating within the eukaryotic cytosol. It was the goal of this research to examine the roles of iscSUA and suf, gene systems predicted to encode for iron-sulfur cluster biosynthesis proteins, in Shigella surviving exposure to oxidative stress agents and during Shigella invasion and plaque formation …