Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
A Method To Detect Oxidative Stress By Monitoring Changes In The Extracellular Antioxidant Capacity In Plant Suspension Cells, C. Jacyn Baker, Norton M. Mock
A Method To Detect Oxidative Stress By Monitoring Changes In The Extracellular Antioxidant Capacity In Plant Suspension Cells, C. Jacyn Baker, Norton M. Mock
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Detection of H2O2 in the supernatant of plant suspension cells is often used to indicate the time and extent of the oxidative burst during interactions with either bacteria or pathogen-related elicitors. We have found that suspensions of plant cells, depending on conditions, may produce considerable levels of extracellular phenolics that can function as antioxidants and prevent or suppress the detection of H2O2. These compounds can be used as substrates by extracellular peroxidases to scavenge stoichiometric amounts of H2O2. When this occurs during plant/pathogen interactions it can mask both the …