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The Occurrence And Characterization Of Hemoglobin From Different Strains Of Genetically Diverse, Free-Living Frankia, Jason Beckwith

Electronic Theses and Dissertations

Hemoglobins have been identified in root nodules of many actinorhizal plants. When cultured in vitro, the actinomycete Frankia strain CcI3 produces hemoglobin when grown with or without supplied nitrogen. The cyanobacterium, Nostoc commune, also produces hemoglobin in vitro, although only under nitrogen-fixing, microaerobic conditions, and in less than one fifth of the explored strainslspecies. The objectives of this study were to determine if Frankia strains EANlpec, ArI3, EUNlf, CcI.17, and Cc13, members of diverse genogroups, are capable of producing hemoglobin in vitro, to characterize the oxygen kinetics of the hemoglobin, and to determine the effect of nitrogen, oxygen, and carbon …