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Mechanistic Studies On Malate Dehydrogenase From Escherichia Coli, S. Kirk Wright
Mechanistic Studies On Malate Dehydrogenase From Escherichia Coli, S. Kirk Wright
Masters Theses
Kinetic studies and chemical modification studies were performed on malate dehydrogenase from Escherichia coli (eMDH). Chemical modification studies using diethylpyrocarbonate and iodoacetic acid, along with log V/K profiles indicate that one ionizing group with a pKa between 7.9 and 8.5 acts as a general acid/base in the catalytic mechanism. Log V profiles suggest that malate binds to the correctly protonated form of the enzyme. These results imply that a histidine residue is required for catalysis.
A primary deuterium kinetic isotope effect of 1.43 (±0.14) was observed on V/K using malate-2-d, while no isotope effect was measured on Vmax …
Small Mammal Community Structure In Restored Prairies, Kathleen A. Falout
Small Mammal Community Structure In Restored Prairies, Kathleen A. Falout
Masters Theses
The Illinois Department of Natural Resources currently manages approximately 4,000 hectares of natural prairie. High human impacts coupled with small patch size result in challenging management decisions for prairie conservation. The ideal restored prairie would consist of a mosaic of habitats varying in vegetation from forb-grass dominated areas to oak savannas, with concurrent stages of vegetative succession occurring across the habitats. A study was conducted at the Middle Fork State Fish and Wildlife Area located in Vermillion County, IL, to analyze small mammal diversity and community composition among three different stages of prairie restoration.
The three treatment categories were: fallow …